primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	ACCTCCAGTTTGATCAGGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.179582	116	541	848-875	1.000000	86.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CTGCCTTAATCCACTTGGCT	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	116	541	874-901	1.000000	85.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACTGCCTTAATCCACTTGGC	ACCTCCAGTTTGATCAGGGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.205369	116	541	849-876	1.000000	85.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CTGCCTTAATCCACTTGGCT	CAGTGCTATGCATGGTTCCT	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.183233	116	541	873-900	1.000000	85.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACTGCCTTAATCCACTTGGC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	58.2	58.0	0.0	10.2	0.203262	116	541	875-902	1.000000	85.6
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	8992	9646	735-811	1.000000	68.6
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	8992	9646	738-814	1.000000	68.4
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	8992	9646	741-817	1.000000	67.9
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	8992	9646	744-820	1.000000	67.7
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	8992	9646	739-815	1.000000	66.2
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	TGGAAAAGCGTCCATTGTCT	ATTCGGGAACAGATGGAACG	20	20	45.0	50.0	57.7	58.0	3.0	0.0	0.294528	9719	10026	432-455	1.000000	84.5
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	TGGAAAAGCGTCCATTGTCT	CGGGAACAGATGGAACGTTT	20	20	45.0	50.0	57.7	58.5	3.0	0.5	0.750944	9719	10026	429-452	1.000000	80.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TTTGGAATGGAAAAGCGTCC	ATTCGGGAACAGATGGAACG	20	20	45.0	50.0	56.9	58.0	0.0	0.0	1.122833	9719	10026	439-462	1.000000	77.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TGGAAAAGCGTCCATTGTCT	GCTATTCGGGAACAGATGGA	20	20	45.0	50.0	57.7	57.1	3.0	0.0	1.181040	9719	10026	435-458	1.000000	77.4
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGAAAAGCGTCCATTGTCT	AGCTATTCGGGAACAGATGG	20	20	45.0	50.0	57.7	57.1	3.0	0.0	1.189064	9719	10026	436-459	1.000000	77.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.2	58.2	1.7	0.0	0.335274	28706	29708	1029-1051	1.000000	80.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28706	29708	1065-1087	1.000000	79.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	CTGCAAATCCTTTTTCCCCG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.520003	28706	29708	1059-1081	1.000000	78.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGTGTCGCCTGATTAACAA	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.2	57.6	1.7	0.0	0.534788	28706	29708	1090-1112	1.000000	78.7
trnC-GCA-petN_p5	trnC-GCA-petN	5	TCCTTTTTCCCCGGTTCAAA	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.607421	28706	29708	1052-1074	1.000000	78.2
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	GCGCTGTTCATTCTTGTTCC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.182245	29799	30903	1217-1260	1.000000	82.5
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	GCGCTGTTCATTCTTGTTCC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.381759	29799	30903	1156-1199	1.000000	81.1
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	58.2	58.3	0.0	25.3	0.408587	29799	30903	1226-1269	1.000000	80.7
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	58.2	57.6	0.0	25.3	0.529793	29799	30903	1227-1270	1.000000	79.7
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	ACTGCGCTGTTCATTCTTGT	20	20	50.0	45.0	58.2	58.4	0.0	14.8	0.553606	29799	30903	1220-1263	1.000000	79.5
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CCATTTCGTGGTGGATCAGT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.119831	32830	33509	1037-1076	1.000000	84.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CCATTTCGTGGTGGATCAGT	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.217397	32830	33509	1002-1041	1.000000	83.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TGCCACGTTGTAACAGAGAC	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.145295	32830	33509	1389-1428	1.000000	83.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CCATTTCGTGGTGGATCAGT	ACTTTTCTGCGGAAGAAGGG	20	20	50.0	50.0	57.9	58.1	0.0	22.8	0.216300	32830	33509	1596-1641	1.000000	82.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGCCACGTTGTAACAGAGAC	ACTTTTCTGCGGAAGAAGGG	20	20	50.0	50.0	58.1	58.1	0.0	22.8	0.241764	32830	33509	1948-1993	1.000000	81.6
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	TTCGAATCCCTCTCTTTCCG	TCCAACACAAAGGATTGGTCA	20	21	50.0	42.9	57.0	57.7	0.0	2.4	2.266437	47827	48122	399-417	1.000000	68.2
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	GGGTTCGAATCCCTCTCTTT	TCCAACACAAAGGATTGGTCA	20	21	50.0	42.9	56.9	57.7	17.2	2.4	2.387466	47827	48122	402-420	1.000000	67.2
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GGGTTCGAATCCCTCTCTTTC	TCCAACACAAAGGATTGGTCA	21	21	52.4	42.9	58.1	57.7	17.2	2.4	2.397276	47827	48122	402-420	1.000000	67.1
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	GGTTCGAATCCCTCTCTTTCC	TCCAACACAAAGGATTGGTCA	21	21	52.4	42.9	58.1	57.7	0.0	2.4	2.397276	47827	48122	401-419	1.000000	67.1
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	GTTCGAATCCCTCTCTTTCCG	TCCAACACAAAGGATTGGTCA	21	21	52.4	42.9	58.5	57.7	0.0	2.4	2.763355	47827	48122	400-418	1.000000	64.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ATGGGGGATAAAACTCCGGA	GCATGTTGGGTCTTTGAAGC	20	20	50.0	50.0	58.1	57.9	2.9	0.0	0.168614	53172	54322	1328-1367	1.000000	83.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCCGGAAATTCGAAATGCCA	GCATGTTGGGTCTTTGAAGC	20	20	45.0	50.0	58.2	57.9	10.9	0.0	0.242580	53172	54322	1314-1353	1.000000	82.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATGGGGGATAAAACTCCGGA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	2.9	0.0	0.295635	53172	54322	1264-1303	1.000000	82.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCCGGAAATTCGAAATGCCA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	58.2	57.8	10.9	0.0	0.369602	53172	54322	1250-1289	1.000000	82.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGGGGATAAAACTCCGGAA	GCATGTTGGGTCTTTGAAGC	20	20	50.0	50.0	58.3	57.9	5.2	0.0	0.398968	53172	54322	1327-1366	1.000000	81.6
atpB-rbcL_p1	atpB-rbcL	1	GCCAATGATTTGAGCGATCC	GCCTTGAATCCAACACTTGC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.482682	57411	58206	922-941	1.000000	77.5
atpB-rbcL_p2	atpB-rbcL	2	GGCCAATGATTTGAGCGATC	GCCTTGAATCCAACACTTGC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.482682	57411	58206	923-942	1.000000	77.5
atpB-rbcL_p3	atpB-rbcL	3	GCCAATGATTTGAGCGATCC	CCAGCCTTGAATCCAACACT	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.497280	57411	58206	925-944	1.000000	77.4
atpB-rbcL_p4	atpB-rbcL	4	GGCCAATGATTTGAGCGATC	CCAGCCTTGAATCCAACACT	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.497280	57411	58206	926-945	1.000000	77.4
atpB-rbcL_p5	atpB-rbcL	5	TTTTCAAACGCGGAAACTCC	GCCTTGAATCCAACACTTGC	20	20	45.0	50.0	57.5	57.9	12.5	0.0	0.548809	57411	58206	888-907	1.000000	77.0
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	ACCAATAAATAGGGCTGACGT	20	21	50.0	42.9	57.8	57.1	24.9	0.0	2.082401	68310	69801	1475-1621	1.000000	69.2
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	ACCAATAAATAGGGCTGACGT	20	21	55.0	42.9	58.2	57.1	0.9	0.0	2.123989	68310	69801	1470-1616	1.000000	68.9
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AGACCAATAAATAGGGCTGACG	20	22	50.0	45.5	57.8	57.9	24.9	0.0	2.229636	68310	69801	1477-1623	1.000000	68.0
psbE-petL_p4	psbE-petL	4	ACCTGCAATGAATAGGGACG	ACCAATAAATAGGGCTGACGT	20	21	50.0	42.9	57.7	57.1	0.0	0.0	2.258121	68310	69801	1550-1696	1.000000	67.7
psbE-petL_p5	psbE-petL	5	CTGCAATGAATAGGGACGGT	ACCAATAAATAGGGCTGACGT	20	21	50.0	42.9	57.7	57.1	0.0	0.0	2.258121	68310	69801	1548-1694	1.000000	67.7
rpl33-rps18_p1	rpl33-rps18	1	AGAATCGACACAATACGCCT	GGGGTCGCTTGGACTTAAAA	20	20	45.0	50.0	57.0	58.1	0.0	0.0	1.061281	72026	72303	407-413	1.000000	77.7
rpl33-rps18_p2	rpl33-rps18	2	CTGTCCCTATTGTTCCAAGCA	GGGGTCGCTTGGACTTAAAA	21	20	47.6	50.0	57.9	58.1	0.0	0.0	1.171628	72026	72303	364-370	1.000000	77.6
rpl33-rps18_p3	rpl33-rps18	3	CGACACAATACGCCTAATCG	GGGGTCGCTTGGACTTAAAA	20	20	50.0	50.0	56.8	58.1	0.0	0.0	1.280574	72026	72303	402-408	1.000000	76.0
rpl33-rps18_p4	rpl33-rps18	4	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.216072	72026	72303	450-456	1.000000	75.9
rpl33-rps18_p5	rpl33-rps18	5	CTGTCCCTATTGTTCCAAGCA	TCAATTCGATCCCCCGATTG	21	20	47.6	50.0	57.9	57.7	0.0	0.0	1.326420	72026	72303	407-413	1.000000	75.6
rps18_p1	rps18	1	AACGATAGCGGCTTTACAGG	CCGGGAGAATGAATTCCGAG	20	20	50.0	55.0	58.1	58.1	8.3	2.3	0.192987	72304	72609	818-825	1.000000	82.3
rps18_p2	rps18	2	AACGATAGCGGCTTTACAGG	ACGAGGATATATAGCGCGGA	20	20	50.0	50.0	58.1	58.0	8.3	8.8	0.098172	72304	72609	1204-1211	1.000000	82.2
rps18_p3	rps18	3	CGGAAAATCGAAGCGTTGAG	ACGAGGATATATAGCGCGGA	20	20	50.0	50.0	57.9	58.0	0.0	8.8	0.179157	72304	72609	1461-1468	1.000000	81.1
rps18_p4	rps18	4	AACGATAGCGGCTTTACAGG	GAAAGTGGAAGGGGTACGTC	20	20	50.0	55.0	58.1	57.9	8.3	0.0	0.154467	72304	72609	868-870	0.800000	72.8
rps18_p5	rps18	5	CGGAAAATCGAAGCGTTGAG	GAAAGTGGAAGGGGTACGTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.235453	72304	72609	1125-1127	0.800000	71.8
rpl20-rps12_p1	rpl20-rps12	1	TCCGCGCTATATATCCTCGT	GAGGAACATGTACTCGGGTG	20	20	50.0	55.0	58.0	57.7	5.6	12.9	0.329357	73254	74073	876-892	1.000000	78.7
rpl20-rps12_p2	rpl20-rps12	2	TCCGCGCTATATATCCTCGT	AGGAACATGTACTCGGGTGT	20	20	50.0	50.0	58.0	58.4	5.6	0.0	0.393188	73254	74073	875-891	1.000000	78.2
rpl20-rps12_p3	rpl20-rps12	3	TCCGCGCTATATATCCTCGT	AAATGTCACCAAATCCCCCG	20	20	50.0	50.0	58.0	58.4	5.6	0.0	0.478138	73254	74073	920-936	1.000000	77.5
rpl20-rps12_p4	rpl20-rps12	4	AGTAAGTCTTGAATGCGCCC	GAGGAACATGTACTCGGGTG	20	20	50.0	55.0	58.3	57.7	4.2	12.9	0.556186	73254	74073	938-954	1.000000	76.8
rpl20-rps12_p5	rpl20-rps12	5	AGTAAGTCTTGAATGCGCCC	AGGAACATGTACTCGGGTGT	20	20	50.0	50.0	58.3	58.4	4.2	0.0	0.620017	73254	74073	937-953	1.000000	76.3
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	113964	114593	765-772	1.000000	79.6
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	113964	114593	802-809	1.000000	79.0
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	57.8	58.1	18.9	0.0	0.311620	113964	114593	806-813	1.000000	78.8
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	57.7	58.0	9.3	0.0	0.343052	113964	114593	819-826	1.000000	78.5
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	TTTTTAGGGTACTCCGGGGA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	57.7	58.0	9.3	0.0	0.343052	113964	114593	820-827	1.000000	78.5
ndhF-rpl32_p1	ndhF-rpl32	1	CTGCAAGTGTAACGAAGGGT	TTTTTCCGATGTCAAGCCGA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.235487	118523	119647	1285-1406	1.000000	84.7
ndhF-rpl32_p2	ndhF-rpl32	2	CGATGCAATAGGTCGTGTGA	TTTTTCCGATGTCAAGCCGA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.193197	118523	119647	1749-1870	1.000000	84.6
ndhF-rpl32_p3	ndhF-rpl32	3	TCGATGCAATAGGTCGTGTG	TTTTTCCGATGTCAAGCCGA	20	20	50.0	45.0	58.1	58.1	6.6	0.0	0.193197	118523	119647	1750-1871	1.000000	84.6
ndhF-rpl32_p4	ndhF-rpl32	4	ATGGGTCAATCAAGTGTCCG	TTTTTCCGATGTCAAGCCGA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.228300	118523	119647	1507-1628	1.000000	84.6
ndhF-rpl32_p5	ndhF-rpl32	5	CGATGCAATAGGTCGTGTGA	AAAAAGCTTTCAACGCGGTC	20	20	50.0	45.0	58.1	58.2	0.0	13.1	0.243636	118523	119647	1646-1767	1.000000	84.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACCGCGTTGAAAGCTTTTTC	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.172709	119822	121186	1401-1504	1.000000	85.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GACCGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.172709	119822	121186	1402-1505	1.000000	85.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACCGCGTTGAAAGCTTTTTC	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.174080	119822	121186	1402-1505	1.000000	85.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GACCGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.174080	119822	121186	1403-1506	1.000000	85.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACCGCGTTGAAAGCTTTTTC	GCATCATTAACCACCGGGAT	20	20	45.0	50.0	58.2	58.0	14.0	6.3	0.183623	119822	121186	2007-2133	1.000000	84.5
ndhA_p1	ndhA	1	TTGTTTGAGACAAGACAGAA	TGGGAGAAGTTGATCGTTGA	20	20	35.0	45.0	52.6	56.5	0.0	0.0	6.919120	127059	129364	2359-2365	1.000000	41.5
ndhA_p2	ndhA	2	TCTCTTGTTTTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	21	20	33.3	45.0	53.5	56.5	0.0	0.0	6.986459	127059	129364	2368-2374	1.000000	41.5
ndhA_p3	ndhA	3	TCTCTTGTTTTGTTTGAGACAA	TGGGAGAAGTTGATCGTTGA	22	20	31.8	45.0	54.4	56.5	0.0	0.0	7.137902	127059	129364	2368-2374	1.000000	41.5
ndhA_p4	ndhA	4	TGTTTGAGACAAGACAGAAT	TGGGAGAAGTTGATCGTTGA	20	20	35.0	45.0	52.3	56.5	0.0	0.0	7.166422	127059	129364	2358-2364	1.000000	41.5
ndhA_p5	ndhA	5	TTCTCTTGTTTTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	22	20	31.8	45.0	54.4	56.5	0.0	0.0	7.137902	127059	129364	2369-2375	1.000000	41.4