primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	4180	5080	1018-1038	1.000000	84.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	4180	5080	1017-1037	1.000000	84.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	57.5	58.0	0.0	9.2	0.465381	4180	5080	1004-1024	1.000000	82.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGCACTTAAAAGCCGAGTAC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	57.2	58.0	0.0	9.2	0.786822	4180	5080	1026-1046	1.000000	79.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	ATTTCCTTGAAAGGGGTGCT	20	20	50.0	45.0	58.2	57.3	20.3	9.2	0.870690	4180	5080	1018-1038	1.000000	79.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTAGAAAGTTTCTCCGGCCC	TGAATCAGAGCACATGGAGC	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.059693	49990	51098	1472-1519	1.000000	87.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTAGAAAGTTTCTCCGGCCC	TTGGTAGTGCTCCTGGATCA	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.074379	49990	51098	1505-1552	1.000000	86.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTAGAAAGTTTCTCCGGCCC	GACAAAAGGTTCGGTCCAGT	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.062416	49990	51098	1777-1823	1.000000	86.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CTAGAAAGTTTCTCCGGCCC	CTGACAAAAGGTTCGGTCCA	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.063795	49990	51098	1779-1825	1.000000	86.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTAGAAAGTTTCTCCGGCCC	TGACAAAAGGTTCGGTCCAG	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.063795	49990	51098	1778-1824	1.000000	86.5
trnV-UAC_p1	trnV-UAC	1	TTTTCAACAGGCTCGTGTCA	TGAATCAGAGCACATGGAGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.065363	51099	51135	550-590	1.000000	90.4
trnV-UAC_p2	trnV-UAC	2	TTTGTTGTGCTTCGCTAGGT	TGAATCAGAGCACATGGAGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.072311	51099	51135	713-753	1.000000	88.8
trnV-UAC_p3	trnV-UAC	3	TTTTCAACAGGCTCGTGTCA	GACAAAAGGTTCGGTCCAGT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.068086	51099	51135	855-894	1.000000	88.2
trnV-UAC_p4	trnV-UAC	4	TTTTCAACAGGCTCGTGTCA	CTGACAAAAGGTTCGGTCCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.069465	51099	51135	857-896	1.000000	88.2
trnV-UAC_p5	trnV-UAC	5	TTTTCAACAGGCTCGTGTCA	TGACAAAAGGTTCGGTCCAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.069465	51099	51135	856-895	1.000000	88.2
trnV-UAC_p1	trnV-UAC	1	TTTTCAACAGGCTCGTGTCA	AAACCAAATGGGACCCATCC	20	20	45.0	50.0	58.0	58.0	0.0	26.7	0.080049	51099	51828	1134-1167	1.000000	87.3
trnV-UAC_p2	trnV-UAC	2	TTTTCAACAGGCTCGTGTCA	CCCCTATTGCTACAGCTGTG	20	20	45.0	55.0	58.0	58.0	0.0	29.6	0.072217	51099	51828	1704-1739	1.000000	86.0
trnV-UAC_p3	trnV-UAC	3	TTTTCAACAGGCTCGTGTCA	CCCTATTGCTACAGCTGTGG	20	20	45.0	55.0	58.0	58.0	0.0	29.6	0.072217	51099	51828	1703-1738	1.000000	86.0
trnV-UAC_p4	trnV-UAC	4	TTTGTTGTGCTTCGCTAGGT	CCCCTATTGCTACAGCTGTG	20	20	45.0	55.0	58.0	58.0	0.0	29.6	0.079165	51099	51828	1867-1902	1.000000	85.4
trnV-UAC_p5	trnV-UAC	5	TTTGTTGTGCTTCGCTAGGT	CCCTATTGCTACAGCTGTGG	20	20	45.0	55.0	58.0	58.0	0.0	29.6	0.079165	51099	51828	1866-1901	1.000000	85.4
trnM-CAU_p1	trnM-CAU	1	GGATGGGTCCCATTTGGTTT	CCGTTGACTCCGGTATCTGA	20	20	50.0	55.0	58.0	58.9	24.8	0.0	0.948281	51944	52015	136-138	1.000000	75.3
trnM-CAU_p2	trnM-CAU	2	GGATGGGTCCCATTTGGTTT	CGTTGACTCCGGTATCTGAT	20	20	50.0	50.0	58.0	56.8	24.8	0.0	1.207273	51944	52015	135-137	1.000000	73.3
trnM-CAU_p3	trnM-CAU	3	GGATGGGTCCCATTTGGTTT	ACGCAGTTCAAAACGGAAAT	20	20	50.0	40.0	58.0	56.9	24.8	0.0	1.196628	51944	52015	196-198	1.000000	72.5
trnM-CAU_p4	trnM-CAU	4	TGGGTCCCATTTGGTTTTCT	CCGTTGACTCCGGTATCTGA	20	20	45.0	55.0	57.2	58.9	6.5	0.0	1.684848	51944	52015	133-135	1.000000	69.5
trnM-CAU_p5	trnM-CAU	5	GGATGGGTCCCATTTGGTTT	CCGTTGACTCCGGTATCTG	20	19	50.0	57.9	58.0	57.4	24.8	0.0	1.691692	51944	52015	136-138	1.000000	69.4
ycf4-cemA_p1	ycf4-cemA	1	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	60123	60597	649-650	1.000000	80.9
ycf4-cemA_p2	ycf4-cemA	2	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	60123	60597	648-649	1.000000	80.9
ycf4-cemA_p3	ycf4-cemA	3	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	60123	60597	575-576	1.000000	80.7
ycf4-cemA_p4	ycf4-cemA	4	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.2	58.1	33.5	0.0	0.298310	60123	60597	604-605	1.000000	79.7
ycf4-cemA_p5	ycf4-cemA	5	GCTATTCCCTTGACCCGTAC	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.312489	60123	60597	652-653	1.000000	79.5
cemA_p1	cemA	1	GACGAATGAGGTGGGGTAAT	ACTATTGTATTGGTGGATCATT	20	22	50.0	31.8	57.0	52.9	0.0	0.0	8.103189	60598	61287	754-754	1.000000	41.1
cemA_p2	cemA	2	GACGAATGAGGTGGGGTAAT	ACTATTGTATTGGTGGATCATTT	20	23	50.0	30.4	57.0	53.8	0.0	0.0	8.246083	60598	61287	754-754	1.000000	41.1
cemA_p3	cemA	3	AGAGTCGACGAATGAGGTGG	ACTATTGTATTGGTGGATCATT	20	22	55.0	31.8	59.2	52.9	10.5	0.0	8.288818	60598	61287	760-760	1.000000	41.1
cemA_p4	cemA	4	GACGAATGAGGTGGGGTAATT	ACTATTGTATTGGTGGATCATT	21	22	47.6	31.8	57.7	52.9	0.0	0.0	8.377875	60598	61287	754-754	1.000000	41.1
cemA_p5	cemA	5	GACGAATGAGGTGGGGTAAT	AGATTACTATTGTATTGGTGGATCA	20	25	50.0	32.0	57.0	55.6	0.0	0.0	8.402944	60598	61287	759-759	1.000000	41.1
cemA-petA_p1	cemA-petA	1	TCTCCGTCACTTGTAGTTAT	GCACTTGAAATGGATGTCCG	20	20	40.0	50.0	53.1	57.5	0.0	0.0	5.404135	61288	61501	359-360	1.000000	45.1
cemA-petA_p2	cemA-petA	2	TCTCCGTCACTTGTAGTTATT	GCACTTGAAATGGATGTCCG	21	20	38.1	50.0	54.1	57.5	0.0	0.0	5.494345	61288	61501	359-360	1.000000	45.1
cemA-petA_p3	cemA-petA	3	TCTCCGTCACTTGTAGTTAT	CGCAAAAATGGGATAGGCAC	20	20	40.0	50.0	53.1	57.8	0.0	0.0	5.062827	61288	61501	375-376	1.000000	44.9
cemA-petA_p4	cemA-petA	4	TCTCCGTCACTTGTAGTTATT	CGCAAAAATGGGATAGGCAC	21	20	38.1	50.0	54.1	57.8	0.0	0.0	5.153038	61288	61501	375-376	1.000000	44.9
cemA-petA_p5	cemA-petA	5	TCTCCGTCACTTGTAGTTATTT	CGCAAAAATGGGATAGGCAC	22	20	36.4	50.0	54.9	57.8	0.0	0.0	5.325564	61288	61501	375-376	1.000000	44.9
rps3-rpl22_p1	rps3-rpl22	1	GATGGCTTTGGGTTGTACCA	TGAACAGCCACCTCTTTTCT	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.111766	81941	81965	108-109	1.000000	71.8
rps3-rpl22_p2	rps3-rpl22	2	TGATGGCTTTGGGTTGTACC	TGAACAGCCACCTCTTTTCT	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.111766	81941	81965	109-110	1.000000	71.8
rps3-rpl22_p3	rps3-rpl22	3	AAGGGAATGATGGCTTTGGG	TGAACAGCCACCTCTTTTCT	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.159573	81941	81965	116-117	1.000000	71.4
rps3-rpl22_p4	rps3-rpl22	4	GATGGCTTTGGGTTGTACCA	TGAACAGCCACCTCTTTTCTT	20	21	50.0	42.9	58.1	57.7	0.0	0.0	1.379262	81941	81965	108-109	1.000000	69.7
rps3-rpl22_p5	rps3-rpl22	5	TGATGGCTTTGGGTTGTACC	TGAACAGCCACCTCTTTTCTT	20	21	50.0	42.9	58.1	57.7	0.0	0.0	1.379262	81941	81965	109-110	1.000000	69.7
rps19-rpl2_p1	rps19-rpl2	1	AATGGTAGATGCACGTGACC	AAAAGTTTGGGTAGAGCCGG	20	20	50.0	50.0	58.0	58.1	22.2	0.0	0.118538	82730	82783	399-404	1.000000	80.4
rps19-rpl2_p2	rps19-rpl2	2	AGGTAGTCCCCTGGTTAACC	AAAAGTTTGGGTAGAGCCGG	20	20	55.0	50.0	58.0	58.1	18.3	0.0	0.131804	82730	82783	915-920	1.000000	80.2
rps19-rpl2_p3	rps19-rpl2	3	GGTAGTCCCCTGGTTAACCT	AAAAGTTTGGGTAGAGCCGG	20	20	55.0	50.0	58.0	58.1	24.8	0.0	0.131804	82730	82783	914-919	1.000000	80.2
rps19-rpl2_p4	rps19-rpl2	4	AATGGTAGATGCACGTGACC	GCGAACCAGAAAAGTTTGGG	20	20	50.0	50.0	58.0	57.9	22.2	0.0	0.158377	82730	82783	408-413	1.000000	80.1
rps19-rpl2_p5	rps19-rpl2	5	AATGGTAGATGCACGTGACC	GGCGAACCAGAAAAGTTTGG	20	20	50.0	50.0	58.0	57.9	22.2	0.0	0.158377	82730	82783	409-414	1.000000	80.1
ndhF-rpl32_p1	ndhF-rpl32	1	ACGAAAGGTATGATCCACGC	TGATGCAGAAGTACGTTTTT	20	20	50.0	35.0	58.1	53.5	0.0	0.0	4.600183	110618	111356	810-813	1.000000	46.1
ndhF-rpl32_p2	ndhF-rpl32	2	ACGAAAGGTATGATCCACGC	TTGATGCAGAAGTACGTTTT	20	20	50.0	35.0	58.1	53.5	0.0	0.0	4.600183	110618	111356	811-814	1.000000	46.1
ndhF-rpl32_p3	ndhF-rpl32	3	ACGAAAGGTATGATCCACGC	TTTGATGCAGAAGTACGTTT	20	20	50.0	35.0	58.1	53.5	0.0	0.0	4.600183	110618	111356	812-815	1.000000	46.1
ndhF-rpl32_p4	ndhF-rpl32	4	ACGAAAGGTATGATCCACGC	TTTTGATGCAGAAGTACGTT	20	20	50.0	35.0	58.1	53.5	0.0	0.0	4.600183	110618	111356	813-816	1.000000	46.0
ndhF-rpl32_p5	ndhF-rpl32	5	ACGAAAGGTATGATCCACGC	TTTTTGATGCAGAAGTACGT	20	20	50.0	35.0	58.1	53.5	0.0	0.0	4.600183	110618	111356	814-817	1.000000	46.0
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TGCTCTAAGGTTGAAAATATCA	20	22	50.0	31.8	57.9	53.2	0.0	0.0	6.955480	112447	112552	182-190	1.000000	46.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GGTAGACACGCTGCTCTTAG	TGCTCTAAGGTTGAAAATATCA	20	22	55.0	31.8	57.8	53.2	0.0	0.0	7.027498	112447	112552	184-192	1.000000	46.8
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTAGACACGCTGCTCTTAGG	TGCTCTAAGGTTGAAAATATCA	20	22	55.0	31.8	57.8	53.2	0.0	0.0	7.027498	112447	112552	183-191	1.000000	46.8
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AATTGGTAGACACGCTGCTC	TGCTCTAAGGTTGAAAATATCA	20	22	50.0	31.8	58.3	53.2	0.0	0.0	7.104350	112447	112552	188-196	1.000000	46.7
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AAATTGGTAGACACGCTGCT	TGCTCTAAGGTTGAAAATATCA	20	22	45.0	31.8	57.8	53.2	0.0	0.0	7.016207	112447	112552	189-197	1.000000	46.6
ccsA-ndhD_p1	ccsA-ndhD	1	TGGATATGCTATTTTGGGGTCA	TCAGTTGAAAAGGTTGAAGT	22	20	40.9	35.0	57.2	52.8	0.0	0.0	7.967258	113510	113755	363-364	1.000000	44.2
ccsA-ndhD_p2	ccsA-ndhD	2	TGGATATGCTATTTTGGGGTCA	TCAGTTGAAAAGGTTGAAGTT	22	21	40.9	33.3	57.2	53.8	0.0	0.0	8.040781	113510	113755	363-364	1.000000	44.2
ccsA-ndhD_p3	ccsA-ndhD	3	TGGATATGCTATTTTGGGGTCA	ATCAGTTGAAAAGGTTGAAGT	22	21	40.9	33.3	57.2	53.5	0.0	0.0	8.273344	113510	113755	364-365	1.000000	44.2
ccsA-ndhD_p4	ccsA-ndhD	4	TGGATATGCTATTTTGGGGTCA	TCACTATCAGTTGAAAAGGTTGA	22	23	40.9	34.8	57.2	55.8	0.0	0.0	8.004625	113510	113755	369-370	1.000000	44.1
ccsA-ndhD_p5	ccsA-ndhD	5	TGGATATGCTATTTTGGGGTCA	CACTATCAGTTGAAAAGGTTGAAGT	22	25	40.9	36.0	57.2	57.7	0.0	0.0	8.069513	113510	113755	368-369	1.000000	44.1
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	CGAGGCCGAATACACTTTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051961	116962	117301	745-752	1.000000	91.6
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	GCTGCCAGATACATTGGTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.090065	116962	117301	853-860	1.000000	90.7
ndhG-ndhI_p3	ndhG-ndhI	3	AAGACCCGATCCCAGAAAGA	ACGAGCTGCCAGATACATTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101991	116962	117301	857-864	1.000000	90.5
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCGATCCCAGAAAGA	ACAAACAATACGAGCTGCCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.156502	116962	117301	866-873	1.000000	90.0
ndhG-ndhI_p5	ndhG-ndhI	5	AAGACCCGATCCCAGAAAGA	CCTAATTGGCGATTTCCCCA	20	20	50.0	50.0	58.0	57.9	0.0	5.1	0.113277	116962	117301	461-461	0.500000	59.1
ndhA_p1	ndhA	1	TCAAAGTGTATTCGGCCTCG	TGGGAGGTTCAATGGCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.098683	117890	120075	2891-2891	1.000000	81.5
ndhA_p2	ndhA	2	TCAAAGTGTATTCGGCCTCG	TTGGGAGGTTCAATGGCAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.098683	117890	120075	2892-2892	1.000000	81.5
ndhA_p3	ndhA	3	TGACCAATGTATCTGGCAGC	TGGGAGGTTCAATGGCAAAA	20	20	50.0	45.0	58.0	57.9	10.3	0.0	0.136786	117890	120075	2783-2783	1.000000	81.3
ndhA_p4	ndhA	4	TGACCAATGTATCTGGCAGC	TTGGGAGGTTCAATGGCAAA	20	20	50.0	45.0	58.0	57.9	10.3	0.0	0.136786	117890	120075	2784-2784	1.000000	81.3
ndhA_p5	ndhA	5	CAATGTATCTGGCAGCTCGT	TGGGAGGTTCAATGGCAAAA	20	20	50.0	45.0	58.1	57.9	10.3	0.0	0.148713	117890	120075	2779-2779	1.000000	81.2
ycf1_p1	ycf1	1	CTGATTGAAGGCGAAGGGTT	GCAGAATACCGTCACCCATT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.212002	126358	126507	823-823	1.000000	86.3
ycf1_p2	ycf1	2	TGAAGGCGAAGGGTTTTTGT	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.268093	126358	126507	818-818	1.000000	85.9
ycf1_p3	ycf1	3	TGATTGAAGGCGAAGGGTTT	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.324751	126358	126507	822-822	1.000000	85.4
ycf1_p4	ycf1	4	CTGATTGAAGGCGAAGGGTT	AAGGCGGATCCGAACAAAAT	20	20	50.0	45.0	58.2	58.2	0.0	37.6	0.339427	126358	126507	683-683	1.000000	85.3
ycf1_p5	ycf1	5	CTGATTGAAGGCGAAGGGTT	TCGAGTTACAGAGACAGGCT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.375536	126358	126507	612-612	1.000000	85.0