primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGGGTATGGCTCCTTAAACG	TTGCGAAGATTAGGTTCGGG	20	20	50.0	50.0	57.3	58.0	0.0	7.3	0.721134	86	1147	1812-1814	0.888889	70.2
psbA_p2	psbA	2	TGGGTATGGCTCCTTAAACG	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	57.3	58.0	0.0	9.6	0.721335	86	1147	1151-1151	0.888889	69.9
psbA_p3	psbA	3	TGGGTATGGCTCCTTAAACG	CATAGGGAAAGTCGTGTGCA	20	20	50.0	50.0	57.3	57.9	0.0	0.0	0.795898	86	1147	1475-1475	0.888889	69.6
psbA_p4	psbA	4	TGGGTATGGCTCCTTAAACG	ACATAGGGAAAGTCGTGTGC	20	20	50.0	50.0	57.3	57.9	0.0	0.0	0.797354	86	1147	1476-1476	0.888889	69.6
psbA_p5	psbA	5	TGGGTATGGCTCCTTAAACG	GTTTGGGGAGGGGTCTTTTT	20	20	50.0	50.0	57.3	57.9	0.0	0.0	0.799702	86	1147	1437-1437	0.888889	69.5
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GTCGTGGCATTAGCACTTTT	20	20	50.0	45.0	57.9	57.0	0.0	0.0	1.107736	13898	14392	604-607	1.000000	72.6
atpF-atpH_p2	atpF-atpH	2	CAGTGGCCTAAGGAAACGAA	GTCGTGGCATTAGCACTTTT	20	20	50.0	45.0	57.8	57.0	3.3	0.0	1.168334	13898	14392	576-579	1.000000	72.2
atpF-atpH_p3	atpF-atpH	3	AGTGGCCTAAGGAAACGAAA	GTCGTGGCATTAGCACTTTT	20	20	45.0	45.0	57.1	57.0	3.3	0.0	1.932458	13898	14392	575-578	1.000000	66.1
atpF-atpH_p4	atpF-atpH	4	CCAGTGGCCTAAGGAAACG	GTCGTGGCATTAGCACTTTT	19	20	57.9	45.0	58.1	57.0	3.3	0.0	2.134900	13898	14392	577-580	1.000000	64.4
atpF-atpH_p5	atpF-atpH	5	AGTGGCCTAAGGAAACGAAAG	GTCGTGGCATTAGCACTTTT	21	20	47.6	45.0	58.2	57.0	3.3	0.0	2.209917	13898	14392	575-578	1.000000	63.8
rpoB_p1	rpoB	1	CCGCGGGGACATATAATTCA	TCCGGAATGGAAATGAGGGA	20	20	50.0	50.0	57.5	58.4	20.1	0.0	0.875270	26989	27108	249-249	1.000000	73.6
rpoB_p2	rpoB	2	CCGCGGGGACATATAATTCA	CCGGAATGGAAATGAGGGAA	20	20	50.0	50.0	57.5	57.6	20.1	0.0	0.901233	26989	27108	248-248	1.000000	73.4
rpoB_p3	rpoB	3	TTCCAAATTAATCCCGCGGG	TCCGGAATGGAAATGAGGGA	20	20	50.0	50.0	58.6	58.4	39.1	0.0	1.014791	26989	27108	262-262	1.000000	72.5
rpoB_p4	rpoB	4	TTCCAAATTAATCCCGCGGG	CCGGAATGGAAATGAGGGAA	20	20	50.0	50.0	58.6	57.6	39.1	0.0	1.040754	26989	27108	261-261	1.000000	72.3
rpoB_p5	rpoB	5	TCCCGCGGGGACATATAATT	TCCGGAATGGAAATGAGGGA	20	20	50.0	50.0	58.6	58.4	42.4	0.0	1.055309	26989	27108	251-251	1.000000	72.2
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.413468	29619	30605	1123-1145	1.000000	70.9
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	29619	30605	1072-1094	1.000000	70.1
petN-psbM_p3	petN-psbM	3	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	29619	30605	1071-1093	1.000000	70.1
petN-psbM_p4	petN-psbM	4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	57.8	57.6	0.0	0.0	1.583671	29619	30605	1070-1092	1.000000	69.6
petN-psbM_p5	petN-psbM	5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.598551	29619	30605	1129-1151	1.000000	69.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.315353	30711	31750	1151-1154	1.000000	62.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	TACACCTCACCGGGATTGTA	20	20	45.0	50.0	55.8	57.8	0.0	6.3	2.464194	30711	31750	1170-1173	1.000000	60.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	CCTCACCGGGATTGTAGTTC	20	20	45.0	55.0	55.8	57.7	0.0	6.3	2.540423	30711	31750	1166-1169	1.000000	60.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAACAGTGCAGTAGC	ACCTCACCGGGATTGTAGTT	20	20	45.0	50.0	55.8	58.3	0.0	6.3	2.576351	30711	31750	1167-1170	1.000000	59.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	ATACACCTCACCGGGATTGT	20	20	45.0	50.0	55.8	58.4	0.0	6.3	2.649239	30711	31750	1171-1174	1.000000	59.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47761	48770	1035-1090	1.000000	83.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47761	48770	1043-1098	1.000000	82.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47761	48770	1067-1122	1.000000	82.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGAAGTTCCCACTTAGCAGG	20	20	55.0	55.0	57.9	57.9	0.0	11.2	0.248083	47761	48770	1096-1151	1.000000	82.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47761	48770	1075-1130	1.000000	82.0
rbcL-accD_p1	rbcL-accD	1	CGCTGCTTGTGAAGTATGGA	CAACATCGAATTGAACCGCC	20	20	50.0	50.0	58.3	58.1	0.0	6.9	0.379308	59080	59877	883-909	1.000000	81.0
rbcL-accD_p2	rbcL-accD	2	CGCTGCTTGTGAAGTATGGA	ACATCGAATTGAACCGCCAT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.524166	59080	59877	881-907	1.000000	79.8
rbcL-accD_p3	rbcL-accD	3	AAATGGAGCCCTGAACTAGC	CAACATCGAATTGAACCGCC	20	20	50.0	50.0	57.6	58.1	0.0	6.9	0.531791	59080	59877	903-929	1.000000	79.7
rbcL-accD_p4	rbcL-accD	4	CGCTGCTTGTGAAGTATGGA	CCGGTATGTCCAAGAGCATT	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.606423	59080	59877	957-983	1.000000	78.8
rbcL-accD_p5	rbcL-accD	5	AAATGGAGCCCTGAACTAGC	ACATCGAATTGAACCGCCAT	20	20	50.0	45.0	57.6	58.2	0.0	0.0	0.676649	59080	59877	901-927	1.000000	78.5
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195812	65425	66581	1269-1291	1.000000	80.4
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.275279	65425	66581	1270-1292	1.000000	79.7
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCCTGTGATCGG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.283774	65425	66581	1321-1343	1.000000	79.6
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	58.7	58.0	17.6	0.0	0.722382	65425	66581	1276-1298	1.000000	76.1
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	58.7	58.1	17.6	0.0	0.801849	65425	66581	1277-1299	1.000000	75.5
psaJ-rpl33_p1	psaJ-rpl33	1	TTGGGTCTTTAGCGGGTCTA	CCGGCGATTCCTTATTGACA	20	20	50.0	50.0	58.1	58.1	0.0	9.3	0.110519	69459	69946	650-657	1.000000	80.5
psaJ-rpl33_p2	psaJ-rpl33	2	ATGGTTTGGGTCTTTAGCGG	CCGGCGATTCCTTATTGACA	20	20	50.0	50.0	58.2	58.1	0.0	9.3	0.217708	69459	69946	655-662	1.000000	79.6
psaJ-rpl33_p3	psaJ-rpl33	3	TGGGTCTTTAGCGGGTCTAT	CCGGCGATTCCTTATTGACA	20	20	50.0	50.0	57.8	58.1	0.0	9.3	0.221908	69459	69946	649-656	1.000000	79.6
psaJ-rpl33_p4	psaJ-rpl33	4	TTGGGTCTTTAGCGGGTCTA	ATATCTAGAAATGCCCGGCG	20	20	50.0	50.0	58.1	57.6	0.0	4.6	0.445283	69459	69946	664-671	1.000000	77.8
psaJ-rpl33_p5	psaJ-rpl33	5	ATGGTTTGGGTCTTTAGCGG	ATATCTAGAAATGCCCGGCG	20	20	50.0	50.0	58.2	57.6	0.0	4.6	0.552473	69459	69946	669-676	1.000000	76.9
rpl20-rps12_p1	rpl20-rps12	1	CTGTTGAGTAGTGGTTCGGG	AATATCACGAAATCCCCCGC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.188077	71247	71987	920-926	1.000000	79.1
rpl20-rps12_p2	rpl20-rps12	2	CTGTTGAGTAGTGGTTCGGG	TCGGGGAACATGTACTAGGG	20	20	55.0	55.0	57.9	58.2	0.0	0.0	0.295928	71247	71987	879-885	1.000000	78.3
rpl20-rps12_p3	rpl20-rps12	3	TTTGTTCGACGTCTCCTAGC	AATATCACGAAATCCCCCGC	20	20	50.0	50.0	57.7	58.1	14.7	0.0	0.450700	71247	71987	853-859	1.000000	77.0
rpl20-rps12_p4	rpl20-rps12	4	TGTTCGACGTCTCCTAGCTA	AATATCACGAAATCCCCCGC	20	20	50.0	50.0	57.6	58.1	14.7	0.0	0.499406	71247	71987	851-857	1.000000	76.7
rpl20-rps12_p5	rpl20-rps12	5	TTTGTTCGACGTCTCCTAGC	TCGGGGAACATGTACTAGGG	20	20	50.0	55.0	57.7	58.2	14.7	0.0	0.558552	71247	71987	812-818	1.000000	76.2
psbB-psbT_p1	psbB-psbT	1	GCGCTAGAACCTTGTTCAGA	CCTTAGGCGGTTCTCGAAAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.169236	76261	76450	390-394	1.000000	80.5
psbB-psbT_p2	psbB-psbT	2	GCGCTAGAACCTTGTTCAGA	TTTAGTCGGAACCTTAGGCG	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.740475	76261	76450	401-405	1.000000	75.9
psbB-psbT_p3	psbB-psbT	3	GGCGCTAGAACCTTGTTCAG	CCTTAGGCGGTTCTCGAAAA	20	20	55.0	50.0	58.9	57.9	7.6	0.0	1.009447	76261	76450	391-395	1.000000	73.8
psbB-psbT_p4	psbB-psbT	4	GCGCTAGAACCTTGTTCAGA	AACCTTAGGCGGTTCTCGAA	20	20	50.0	50.0	57.9	59.0	0.0	0.0	1.108900	76261	76450	392-396	1.000000	73.0
psbB-psbT_p5	psbB-psbT	5	GCGCTAGAACCTTGTTCAGA	ACCTTAGGCGGTTCTCGAAA	20	20	50.0	50.0	57.9	59.0	0.0	0.0	1.108900	76261	76450	391-395	1.000000	73.0
rpl2_p1	rpl2	1	CGGATGTAGCCAAGTGGATC	CGCTTCAACCGGGTTATTCT	20	20	55.0	50.0	58.1	58.3	0.2	13.0	0.394757	86756	88246	1679-1679	1.000000	77.0
rpl2_p2	rpl2	2	TGGATCAAGGCAGTGGATTG	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.9	58.3	0.0	13.0	0.399119	86756	88246	1665-1665	1.000000	77.0
rpl2_p3	rpl2	3	GGATCAAGGCAGTGGATTGT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.9	58.3	0.0	13.0	0.400626	86756	88246	1664-1664	1.000000	77.0
rpl2_p4	rpl2	4	GTGGATCAAGGCAGTGGATT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.9	58.3	0.0	13.0	0.400626	86756	88246	1666-1666	1.000000	77.0
rpl2_p5	rpl2	5	CGGATGTAGCCAAGTGGATC	ATCATTACGCTTCAACCGGG	20	20	55.0	50.0	58.1	58.3	0.2	6.3	0.465225	86756	88246	1686-1686	1.000000	76.5
ndhD_p1	ndhD	1	AGACTTTGCTTTCTGTCTCA	TGGCCAAAACGTATCTTGTCT	20	21	40.0	42.9	54.5	57.9	0.0	0.6	4.601550	118311	119816	1564-1564	1.000000	43.6
ndhD_p2	ndhD	2	AAGACTTTGCTTTCTGTCTCA	TGGCCAAAACGTATCTTGTCT	21	21	38.1	42.9	55.4	57.9	0.0	0.6	4.758903	118311	119816	1565-1565	1.000000	42.4
ndhD_p3	ndhD	3	AGACTTTGCTTTCTGTCTCAT	TGGCCAAAACGTATCTTGTCT	21	21	38.1	42.9	55.2	57.9	0.0	0.6	4.984526	118311	119816	1564-1564	1.000000	40.6
ndhD_p4	ndhD	4	AGACTTTGCTTTCTGTCTCA	TGGCCAAAACGTATCTTGTC	20	20	40.0	45.0	54.5	56.4	0.0	0.6	5.116825	118311	119816	1564-1564	1.000000	40.5
ndhD_p5	ndhD	5	AGACTTTGCTTTCTGTCTCATT	TGGCCAAAACGTATCTTGTCT	22	21	36.4	42.9	55.9	57.9	0.0	0.6	5.205037	118311	119816	1564-1564	1.000000	40.5
ycf1_p1	ycf1	1	CCACTCGGGGAATTGCAATA	AGGGGAAAGTGAACGAATAGC	20	21	50.0	47.6	57.9	58.0	13.4	0.0	1.064102	129841	129960	175-175	1.000000	75.6
ycf1_p2	ycf1	2	CAAATCCTCGTACCACTCGG	AGGGGAAAGTGAACGAATAGC	20	21	55.0	47.6	58.1	58.0	0.0	0.0	1.090198	129841	129960	187-187	1.000000	75.1
ycf1_p3	ycf1	3	CCACTCGGGGAATTGCAATA	GGGGAAAGTGAACGAATAGCA	20	21	50.0	47.6	57.9	58.3	13.4	0.0	1.344444	129841	129960	174-174	1.000000	73.4
ycf1_p4	ycf1	4	CCACTCGGGGAATTGCAATA	AAGAAGGGGAAAGTGAACGA	20	20	50.0	45.0	57.9	56.7	13.4	0.0	1.367303	129841	129960	179-179	1.000000	73.1
ycf1_p5	ycf1	5	CCACTCGGGGAATTGCAATA	AGAAGGGGAAAGTGAACGAA	20	20	50.0	45.0	57.9	56.7	13.4	0.0	1.367303	129841	129960	178-178	1.000000	73.1
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.3	58.1	13.0	0.2	0.394757	155988	157478	1679-1679	1.000000	77.0
rpl2_p2	rpl2	2	CGCTTCAACCGGGTTATTCT	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.399119	155988	157478	1665-1665	1.000000	77.0
rpl2_p3	rpl2	3	CGCTTCAACCGGGTTATTCT	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.400626	155988	157478	1664-1664	1.000000	77.0
rpl2_p4	rpl2	4	CGCTTCAACCGGGTTATTCT	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.400626	155988	157478	1666-1666	1.000000	77.0
rpl2_p5	rpl2	5	ATCATTACGCTTCAACCGGG	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.3	58.1	6.3	0.2	0.465225	155988	157478	1686-1686	1.000000	76.5