primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	114	531	512-547	1.000000	82.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	114	531	526-561	1.000000	82.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	114	531	525-560	1.000000	82.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	114	531	527-562	1.000000	82.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	114	531	513-548	1.000000	82.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4385	5200	1012-1045	1.000000	79.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4385	5200	1011-1044	1.000000	79.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4385	5200	952-985	1.000000	78.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4385	5200	951-984	1.000000	78.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4385	5200	1036-1069	1.000000	78.2
rps16_p1	rps16	1	AGAAATCACTATCCTACCCTTAGTT	TCTCTGTACCGACTGAACCA	25	20	36.0	50.0	56.8	57.7	0.0	0.0	6.519820	5201	6319	1237-1244	1.000000	41.2
rps16_p2	rps16	2	AGAAATCACTATCCTACCCTTAGT	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	56.1	57.7	0.0	0.0	6.184942	5201	6319	1237-1244	0.900000	37.1
rps16_p3	rps16	3	AGAAATCACTATCCTACCCT	TCTCTGTACCGACTGAACCA	20	20	40.0	50.0	52.0	57.7	0.0	0.0	6.245418	5201	6319	1237-1244	0.900000	37.1
rps16_p4	rps16	4	AGAAATCACTATCCTACCCTT	TCTCTGTACCGACTGAACCA	21	20	38.1	50.0	53.0	57.7	0.0	0.0	6.255365	5201	6319	1237-1244	0.900000	37.1
rps16_p5	rps16	5	AGAAATCACTATCCTACCCTTAGT	GTCTCTGTACCGACTGAACC	24	20	37.5	55.0	56.1	57.4	0.0	0.0	6.536603	5201	6319	1238-1245	0.900000	37.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6320	7290	1039-1062	1.000000	81.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6320	7290	1054-1077	1.000000	79.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6320	7290	1053-1076	1.000000	79.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6320	7290	1043-1066	1.000000	76.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6320	7290	1069-1092	1.000000	76.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9476	10325	891-906	1.000000	81.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9476	10325	909-924	1.000000	78.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TAGTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.6	58.2	0.0	0.0	0.789555	9476	10325	947-962	1.000000	76.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9476	10325	890-905	1.000000	75.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9476	10325	899-914	1.000000	75.1
psaA-ycf3_p1	psaA-ycf3	1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.487535	41562	42314	924-945	1.000000	78.8
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.488764	41562	42314	926-947	1.000000	78.8
psaA-ycf3_p3	psaA-ycf3	3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	20	20	55.0	45.0	57.8	57.5	18.5	0.0	0.716540	41562	42314	925-946	1.000000	77.0
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.745852	41562	42314	859-880	1.000000	77.0
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.747080	41562	42314	861-882	1.000000	77.0
trnF-ndhJ_p1	trnF-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	48140	48713	671-688	1.000000	71.8
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	48140	48713	670-687	1.000000	71.8
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	48140	48713	678-695	1.000000	71.2
trnF-ndhJ_p4	trnF-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	48140	48713	672-689	1.000000	69.7
trnF-ndhJ_p5	trnF-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	48140	48713	671-688	1.000000	68.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	4.6	0.0	0.246856	50386	51601	961-1366	1.000000	83.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.7	58.1	4.6	0.0	0.418656	50386	51601	952-1357	1.000000	81.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.4	58.1	0.0	0.0	0.466616	50386	51601	969-1374	1.000000	81.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.575098	50386	51601	970-1375	1.000000	80.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTCGGAGCTAAAAATCCCGA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	9.7	0.0	0.704436	50386	51601	951-1356	1.000000	79.6
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	63294	64109	950-973	1.000000	78.1
petA-psbJ_p2	petA-psbJ	2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	63294	64109	988-1011	1.000000	76.4
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	63294	64109	987-1010	1.000000	76.4
petA-psbJ_p4	petA-psbJ	4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.754934	63294	64109	956-979	1.000000	75.4
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.761562	63294	64109	955-978	1.000000	75.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.2	6.4	0.0	0.259329	64901	66177	1421-1474	1.000000	79.1
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.2	6.4	0.0	0.264322	64901	66177	1419-1472	1.000000	79.1
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.2	24.9	0.0	0.364337	64901	66177	1344-1397	1.000000	78.3
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.2	24.9	0.0	0.369331	64901	66177	1342-1395	1.000000	78.3
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.2	0.0	0.0	0.379015	64901	66177	1430-1483	1.000000	78.1
rpl20-rps12_p1	rpl20-rps12	1	TCTCCGAGCGATATATCCCC	CAGCGTCGAGGAACATGTAC	20	20	55.0	55.0	57.9	58.7	7.1	9.8	0.785066	69182	69928	776-814	1.000000	78.8
rpl20-rps12_p2	rpl20-rps12	2	TCTCCGAGCGATATATCCCC	AGCGTCGAGGAACATGTACT	20	20	55.0	50.0	57.9	58.8	7.1	9.8	0.879449	69182	69928	775-813	1.000000	78.0
rpl20-rps12_p3	rpl20-rps12	3	TCTCCGAGCGATATATCCCC	TCAGCGTCGAGGAACATGTA	20	20	55.0	50.0	57.9	58.8	7.1	9.8	0.883790	69182	69928	777-815	1.000000	78.0
rpl20-rps12_p4	rpl20-rps12	4	TCTCCGAGCGATATATCCCC	GCGTCGAGGAACATGTACTA	20	20	55.0	50.0	57.9	57.2	7.1	9.8	0.892618	69182	69928	774-812	1.000000	77.9
rpl20-rps12_p5	rpl20-rps12	5	TTCTACGTCTCCGAGCGATA	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.8	58.7	15.9	9.8	0.960509	69182	69928	783-821	1.000000	77.3
rpl16_p1	rpl16	1	AAACCTGCACCCGCTATTTT	TGAACAATTATAATTCTATAGGGTTGA	20	27	45.0	25.9	58.4	54.8	0.0	7.8	10.610371	81459	82897	1542-1576	1.000000	41.4
rpl16_p2	rpl16	2	AACCTGCACCCGCTATTTTT	TGAACAATTATAATTCTATAGGGTTGA	20	27	45.0	25.9	58.4	54.8	0.0	7.8	10.610371	81459	82897	1541-1575	1.000000	41.4
rpl16_p3	rpl16	3	AAACCTGCACCCGCTATTTT	TGAACAATTATAATTCTATAGGGTTG	20	26	45.0	26.9	58.4	53.5	0.0	7.8	10.905110	81459	82897	1542-1576	1.000000	41.4
rpl16_p4	rpl16	4	AACCTGCACCCGCTATTTTT	TGAACAATTATAATTCTATAGGGTTG	20	26	45.0	26.9	58.4	53.5	0.0	7.8	10.905110	81459	82897	1541-1575	1.000000	41.4
rpl16_p5	rpl16	5	GAAACCTGCACCCGCTATTT	TGAACAATTATAATTCTATAGGGTTGA	20	27	50.0	25.9	58.8	54.8	0.0	7.8	11.064322	81459	82897	1543-1577	1.000000	41.4
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CGCCCAATATCCCTTCCTTT	20	20	50.0	50.0	58.0	57.6	17.5	0.0	0.397386	111768	112863	1242-1296	1.000000	80.2
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CGCCCAATATCCCTTCCTTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	111768	112863	1240-1294	1.000000	80.1
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CGCCCAATATCCCTTCCTTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	111768	112863	1241-1295	1.000000	80.1
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	20	20	50.0	45.0	58.0	57.6	17.5	9.5	0.425347	111768	112863	1260-1314	1.000000	80.0
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	20	20	45.0	45.0	58.0	57.6	17.5	9.5	0.436636	111768	112863	1258-1312	1.000000	79.9
rpl32-trnL_p1	rpl32-trnL	1	AAAGGAAGGGATATTGGGCG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	113029	113844	919-1024	1.000000	81.7
rpl32-trnL_p2	rpl32-trnL	2	AAAGGAAGGGATATTGGGCG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	113029	113844	920-1025	1.000000	81.7
rpl32-trnL_p3	rpl32-trnL	3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	113029	113844	901-1006	1.000000	81.5
rpl32-trnL_p4	rpl32-trnL	4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	113029	113844	902-1007	1.000000	81.5
rpl32-trnL_p5	rpl32-trnL	5	AAAGGAAGGGATATTGGGCG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.489250	113029	113844	929-1034	1.000000	80.7
ccsA_p1	ccsA	1	GCGGCATGCCATCTTCTAAA	TGATTCACACGGTTCTCGC	20	19	50.0	52.6	59.0	58.2	34.9	0.0	2.153423	114032	115000	1171-1171	1.000000	63.3
ccsA_p2	ccsA	2	GCGGCATGCCATCTTCTAA	TGATTCACACGGTTCTCGC	19	19	52.6	52.6	58.3	58.2	34.9	0.0	2.471058	114032	115000	1171-1171	1.000000	60.8
ccsA_p3	ccsA	3	CGGCATGCCATCTTCTAAAAG	TGATTCACACGGTTCTCGC	21	19	47.6	52.6	57.7	58.2	34.9	0.0	2.522598	114032	115000	1170-1170	1.000000	60.4
ccsA_p4	ccsA	4	CGGCATGCCATCTTCTAAAA	TGATTCACACGGTTCTCGC	20	19	45.0	52.6	56.5	58.2	34.9	0.0	2.696756	114032	115000	1170-1170	1.000000	59.0
ccsA_p5	ccsA	5	GCGGCATGCCATCTTCTAAA	GATTCACACGGTTCTCGCC	20	19	50.0	57.9	59.0	58.9	34.9	0.0	2.896680	114032	115000	1170-1170	1.000000	57.4
rps15-ycf1_p1	rps15-ycf1	1	ACTTGAAATTCAACAGATCCTCT	AATAATGGGAGCCGCTTGAG	23	20	34.8	50.0	55.8	58.0	4.0	0.0	5.186281	122957	123368	543-549	1.000000	41.6
rps15-ycf1_p2	rps15-ycf1	2	CAGATCCTCTGTTTTCTTTGT	AATAATGGGAGCCGCTTGAG	21	20	38.1	50.0	53.7	58.0	0.0	0.0	5.282521	122957	123368	530-536	1.000000	41.6
rps15-ycf1_p3	rps15-ycf1	3	CAGATCCTCTGTTTTCTTTGTT	AATAATGGGAGCCGCTTGAG	22	20	36.4	50.0	54.6	58.0	0.0	0.0	5.445299	122957	123368	530-536	1.000000	41.6
rps15-ycf1_p4	rps15-ycf1	4	ACTTGAAATTCAACAGATCCTCT	CAATAATGGGAGCCGCTTGA	23	20	34.8	50.0	55.8	58.3	4.0	0.0	5.480014	122957	123368	544-550	1.000000	41.6
rps15-ycf1_p5	rps15-ycf1	5	CAGATCCTCTGTTTTCTTTGT	CAATAATGGGAGCCGCTTGA	21	20	38.1	50.0	53.7	58.3	0.0	0.0	5.576254	122957	123368	531-537	1.000000	41.6
ycf1_p1	ycf1	1	TCATTTCTTCTTCTGCCTGTGA	GACTATAAAGTCGAAATCAGAAA	22	23	40.9	30.4	57.6	52.4	0.0	0.0	10.965959	125668	125817	253-295	1.000000	42.5
ycf1_p2	ycf1	2	TCATTTCTTCTTCTGCCTGTGA	GACTATAAAGTCGAAATCAGAAAA	22	24	40.9	29.2	57.6	53.2	0.0	0.0	11.147725	125668	125817	253-295	1.000000	42.5
ycf1_p3	ycf1	3	CATTTCTTCTTCTGCCTGTGA	GACTATAAAGTCGAAATCAGAAA	21	23	42.9	30.4	56.2	52.4	0.0	0.0	11.439620	125668	125817	252-294	1.000000	42.5
ycf1_p4	ycf1	4	TCATTTCTTCTTCTGCCTGTG	GACTATAAAGTCGAAATCAGAAA	21	23	42.9	30.4	56.2	52.4	0.0	0.0	11.439620	125668	125817	253-295	1.000000	42.5
ycf1_p5	ycf1	5	TCATTTCTTCTTCTGCCTGTGA	GACTATAAAGTCGAAATCAGAAAAT	22	25	40.9	28.0	57.6	53.8	0.0	0.0	11.586086	125668	125817	253-295	1.000000	42.5