primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5713	6992	1242-1353	1.000000	83.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5713	6992	1253-1364	1.000000	82.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5713	6992	1246-1357	1.000000	78.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	5713	6992	1272-1383	1.000000	78.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	5713	6992	1254-1365	1.000000	77.9
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CTTATCTAACGATCCGGGCC	ATTGGGAGAGATGGCTGAGT	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.474855	8163	8277	250-254	1.000000	77.5
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CTTATCTAACGATCCGGGCC	GGGTTCGAATCCCTCTCTCT	20	20	55.0	55.0	57.6	58.2	0.0	17.2	0.582336	8163	8277	181-185	1.000000	77.1
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CTTATCTAACGATCCGGGCC	GTGGACTAAAGCGGCAGATT	20	20	55.0	50.0	57.6	58.3	0.0	1.4	0.626341	8163	8277	232-236	1.000000	76.4
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CTTATCTAACGATCCGGGCC	TGGACTAAAGCGGCAGATTG	20	20	55.0	50.0	57.6	58.3	0.0	1.4	0.627569	8163	8277	231-235	1.000000	76.3
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CTTATCTAACGATCCGGGCC	TTCGAATCCCTCTCTCTCCG	20	20	55.0	55.0	57.6	58.3	0.0	0.0	0.683870	8163	8277	178-182	1.000000	76.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	GATCAGAGGGAACGGAGAGA	ACGAATCGCACTTTTACCACT	20	21	55.0	42.9	57.9	58.2	0.0	1.2	1.297305	8366	9697	531-1466	1.000000	74.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AAGTGATCAGAGGGAACGGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.1	58.2	0.0	1.2	1.298670	8366	9697	535-1470	1.000000	74.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATCAGAGGGAACGGAGAGAG	ACGAATCGCACTTTTACCACT	20	21	55.0	42.9	57.9	58.2	0.0	1.2	1.303153	8366	9697	530-1465	1.000000	74.7
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GATCAGAGGGAACGGAGAGA	ACGAATCGCACTTTTACCAC	20	20	55.0	45.0	57.9	56.8	0.0	1.2	1.304330	8366	9697	530-1466	1.000000	74.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AAGTGATCAGAGGGAACGGA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.1	56.8	0.0	1.2	1.305695	8366	9697	534-1470	1.000000	74.7
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.156700	13736	14234	621-622	1.000000	79.3
atpF-atpH_p2	atpF-atpH	2	CAGTGGCCTAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.8	58.0	3.3	0.0	0.217299	13736	14234	593-594	1.000000	78.8
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	20	20	50.0	45.0	57.9	58.3	0.0	18.7	0.390245	13736	14234	608-609	1.000000	77.4
atpF-atpH_p4	atpF-atpH	4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	20	20	50.0	45.0	57.8	58.3	3.3	18.7	0.450844	13736	14234	580-581	1.000000	76.9
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	20	20	50.0	45.0	57.9	57.2	0.0	18.7	0.874150	13736	14234	607-608	1.000000	73.5
rpoC1_p1	rpoC1	1	GGCACCGGTATCGTCTAATC	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	57.9	58.0	6.9	23.5	0.101993	21184	23971	1848-2939	1.000000	79.7
rpoC1_p2	rpoC1	2	GCACCGGTATCGTCTAATCG	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	58.3	58.0	6.9	23.5	0.357271	21184	23971	1847-2938	1.000000	77.7
rpoC1_p3	rpoC1	3	GGCACCGGTATCGTCTAATC	GCTCGTTCGAGAACTACGAT	20	20	55.0	50.0	57.9	57.5	6.9	23.5	0.517035	21184	23971	1847-2937	1.000000	76.4
rpoC1_p4	rpoC1	4	TTGGATAGACCCGTCTTCCC	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	58.5	58.0	16.6	23.5	0.547489	21184	23971	1871-2962	1.000000	76.1
rpoC1_p5	rpoC1	5	GCACCGGTATCGTCTAATCG	GCTCGTTCGAGAACTACGAT	20	20	55.0	50.0	58.3	57.5	6.9	23.5	0.772312	21184	23971	1846-2936	1.000000	74.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGCCAAGCCCTGATTAATGA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.3	57.5	0.0	14.6	0.845966	27220	28473	1385-1394	1.000000	75.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	57.5	0.0	14.6	0.934514	27220	28473	1313-1322	1.000000	74.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.6	57.5	0.0	14.6	0.960477	27220	28473	1314-1323	1.000000	74.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGCCAAGCCCTGATTAATGA	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.3	58.8	0.0	0.0	1.086040	27220	28473	1422-1431	1.000000	73.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CGCCAAGCCCTGATTAATGA	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	58.3	58.8	0.0	0.7	1.155591	27220	28473	1408-1417	1.000000	72.6
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	57.5	58.0	14.7	0.0	0.531149	28545	29426	963-966	1.000000	77.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTA	AGCAATTAGTAGTGCCCAGC	20	20	50.0	50.0	57.5	58.0	14.7	4.7	0.569228	28545	29426	1040-1043	1.000000	76.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	20	20	50.0	55.0	57.5	58.1	14.7	0.0	0.644164	28545	29426	1015-1018	1.000000	76.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	20	20	50.0	55.0	57.5	58.1	14.7	0.0	0.644164	28545	29426	1014-1017	1.000000	76.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	20	20	50.0	55.0	57.5	57.8	14.7	0.0	0.701352	28545	29426	1016-1019	1.000000	75.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGTCCTTCACTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.423885	50967	52629	112-1809	1.000000	78.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGTCCTTCACTAATCGGAGC	AGCCCAATGCAACAAAATGC	20	20	55.0	45.0	57.8	58.5	0.0	0.0	0.700661	50967	52629	113-1891	1.000000	76.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGTCCTTCACTAATCGGAGC	GAAGGTCTACGGTTCGAGTC	20	20	55.0	55.0	57.8	57.5	0.0	0.0	0.767657	50967	52629	113-1808	1.000000	75.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGTCCTTCACTAATCGGAGC	GTTCGAGTCCGTATAGCCCT	20	20	55.0	55.0	57.8	58.7	0.0	0.0	0.909951	50967	52629	112-1797	1.000000	74.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGTCCTTCACTAATCGGAGC	GAAAGAGCCCAATGCAACAA	20	20	55.0	45.0	57.8	57.2	0.0	0.0	1.044732	50967	52629	113-1896	1.000000	73.6
psaI-ycf4_p1	psaI-ycf4	1	GCGATTGCAATGGCTTCTTT	ACTTGCTTTTCGAGACCCTG	20	20	45.0	50.0	58.0	58.1	31.7	0.0	0.116194	60385	60732	113-462	1.000000	80.7
psaI-ycf4_p2	psaI-ycf4	2	GCGATTGCAATGGCTTCTTT	CTTGCTTTTCGAGACCCTGT	20	20	45.0	50.0	58.0	58.1	31.7	0.0	0.116194	60385	60732	112-461	1.000000	80.7
psaI-ycf4_p3	psaI-ycf4	3	TTTTGTCCCTTTAGTGGGCC	ACTTGCTTTTCGAGACCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.119702	60385	60732	114-493	1.000000	80.6
psaI-ycf4_p4	psaI-ycf4	4	TTTTGTCCCTTTAGTGGGCC	CTTGCTTTTCGAGACCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.119702	60385	60732	113-492	1.000000	80.6
psaI-ycf4_p5	psaI-ycf4	5	GTGGGCCTAGTATTTCCTGC	CTTGCTTTTCGAGACCCTGT	20	20	55.0	50.0	58.0	58.1	4.5	0.0	0.147061	60385	60732	113-479	1.000000	80.4
petA-psbJ_p1	petA-psbJ	1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.119715	63941	64988	113-1156	1.000000	80.6
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195812	63941	64988	113-1173	1.000000	79.9
petA-psbJ_p3	petA-psbJ	3	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199182	63941	64988	112-1157	1.000000	79.9
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.275279	63941	64988	112-1174	1.000000	79.3
petA-psbJ_p5	petA-psbJ	5	TGTTCTTCTTGGCATCCGTT	CCGGATTGGGTTCATCTCTG	20	20	45.0	55.0	57.7	58.0	0.0	0.0	0.311310	63941	64988	113-1166	1.000000	79.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.5	6.4	0.0	0.541273	65758	66718	113-1107	1.000000	80.0
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.5	0.0	0.0	0.578888	65758	66718	115-1120	1.000000	79.7
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	20	20	45.0	45.0	58.2	57.5	0.0	0.0	0.660959	65758	66718	114-1116	1.000000	79.1
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.677289	65758	66718	113-1115	1.000000	78.9
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.727231	65758	66718	114-1109	1.000000	78.5
ycf2_p1	ycf2	1	TTCTATAGGAACTGGGCGGT	ATCGGTCTATTTTCGGCGTC	20	20	50.0	50.0	57.8	58.2	0.0	6.8	0.320916	92419	92538	114-251	1.000000	84.0
ycf2_p2	ycf2	2	TCTATAGGAACTGGGCGGTC	ATCGGTCTATTTTCGGCGTC	20	20	55.0	50.0	58.3	58.2	0.0	6.8	0.449948	92419	92538	113-250	1.000000	83.0
ycf2_p3	ycf2	3	TTCTATAGGAACTGGGCGGT	AATCGGTCTATTTTCGGCGT	20	20	50.0	45.0	57.8	57.7	0.0	6.8	0.474763	92419	92538	113-252	1.000000	82.7
ycf2_p4	ycf2	4	TTCTATAGGAACTGGGCGGT	CGGTCTATTTTCGGCGTCAT	20	20	50.0	50.0	57.8	58.4	0.0	6.8	0.602508	92419	92538	113-249	1.000000	81.8
ycf2_p5	ycf2	5	TCTATAGGAACTGGGCGGTC	AATCGGTCTATTTTCGGCGT	20	20	55.0	45.0	58.3	57.7	0.0	6.8	0.603796	92419	92538	112-251	1.000000	81.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCTGTCGGAATAAGCA	ACTGACTTTGCTAAAGAAAAAGCT	20	24	45.0	33.3	57.8	57.5	7.1	13.3	4.634189	113292	114282	116-1209	1.000000	44.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCTGTCGGAATAAGCA	ACTGACTTTGCTAAAGAAAAAGC	20	23	45.0	34.8	57.8	56.2	7.1	13.3	4.970056	113292	114282	116-1209	1.000000	42.0
ndhF-rpl32_p3	ndhF-rpl32	3	TGCTGTCGGAATAAGCAGAA	ACTGACTTTGCTAAAGAAAAAGCT	20	24	45.0	33.3	57.2	57.5	7.1	13.3	5.217430	113292	114282	116-1206	1.000000	41.8
ndhF-rpl32_p4	ndhF-rpl32	4	TTGCTGTCGGAATAAGCAGA	ACTGACTTTGCTAAAGAAAAAGCT	20	24	45.0	33.3	57.2	57.5	10.1	13.3	5.217430	113292	114282	117-1207	1.000000	41.8
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCTGTCGGAATAAGCA	CTGACTTTGCTAAAGAAAAAGCT	20	23	45.0	34.8	57.8	55.9	7.1	13.3	5.229486	113292	114282	115-1208	1.000000	41.8
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.019370	115544	116515	114-1482	1.000000	81.4
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.020741	115544	116515	113-1481	1.000000	81.4
ccsA_p3	ccsA	3	GAGTGGCGGCATACTGTAAA	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	17.2	0.0	0.030082	115544	116515	113-1422	1.000000	81.3
ccsA_p4	ccsA	4	GTGAAATCGGTAGACACGCT	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	115544	116515	114-1706	1.000000	81.2
ccsA_p5	ccsA	5	TGAAATCGGTAGACACGCTG	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024937	115544	116515	113-1705	1.000000	81.2
ccsA-ndhD_p1	ccsA-ndhD	1	CGAATTCGTCACTTGTAGCG	TCGCTATCAGTTGACAAGGT	20	20	50.0	45.0	57.3	56.6	19.7	0.0	2.098320	116516	116737	112-395	1.000000	66.5
ccsA-ndhD_p2	ccsA-ndhD	2	CGAATTCGTCACTTGTAGCG	TTCGCTATCAGTTGACAAGGT	20	21	50.0	42.9	57.3	57.3	19.7	0.0	2.356500	116516	116737	113-396	1.000000	64.4
ccsA-ndhD_p3	ccsA-ndhD	3	ATTCGTCACTTGTAGCGTCT	TCGCTATCAGTTGACAAGGT	20	20	45.0	45.0	57.0	56.6	0.0	0.0	2.431571	116516	116737	114-392	1.000000	63.9
ccsA-ndhD_p4	ccsA-ndhD	4	CGAATTCGTCACTTGTAGCG	TCAGTTGACAAGGTACAAGCT	20	21	50.0	42.9	57.3	57.2	19.7	0.0	2.516687	116516	116737	113-389	1.000000	63.2
ccsA-ndhD_p5	ccsA-ndhD	5	CGAATTCGTCACTTGTAGCG	TTTCGCTATCAGTTGACAAGGT	20	22	50.0	40.9	57.3	58.0	19.7	0.0	2.681349	116516	116737	114-397	1.000000	61.8
ndhG-ndhI_p1	ndhG-ndhI	1	ACTCCATCTCCAACAGTCCA	AGCAGCAAGGTACATTGGTC	20	20	50.0	50.0	58.0	58.2	0.0	20.9	0.203642	120030	120409	115-1169	1.000000	79.6
ndhG-ndhI_p2	ndhG-ndhI	2	ACTCCATCTCCAACAGTCCA	GAGCAGCAAGGTACATTGGT	20	20	50.0	50.0	58.0	58.2	0.0	18.4	0.203642	120030	120409	114-1170	1.000000	79.6
ndhG-ndhI_p3	ndhG-ndhI	3	ACTCCATCTCCAACAGTCCA	AACAATACGAGCAGCAAGGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.215031	120030	120409	114-1178	1.000000	79.5
ndhG-ndhI_p4	ndhG-ndhI	4	ACTCCATCTCCAACAGTCCA	ACCCCCTTGATTCAAGAACG	20	20	50.0	50.0	58.0	57.8	0.0	24.7	0.230400	120030	120409	115-711	0.800000	72.0
ndhG-ndhI_p5	ndhG-ndhI	5	ACTCCATCTCCAACAGTCCA	CCCCTTGATTCAAGAACGGT	20	20	50.0	50.0	58.0	57.8	0.0	24.7	0.230400	120030	120409	114-709	0.800000	72.0
ycf1_p1	ycf1	1	AGAAAAGGATAGGTGTGGTGA	CCATTTCCAACGCTGAAACA	21	20	42.9	45.0	56.3	57.2	0.0	0.0	3.524819	127054	127173	114-256	1.000000	53.9
ycf1_p2	ycf1	2	AAGAAAAGGATAGGTGTGGTGA	CCATTTCCAACGCTGAAACA	22	20	40.9	45.0	57.0	57.2	0.0	0.0	3.785088	127054	127173	115-257	1.000000	51.8
ycf1_p3	ycf1	3	AGAAAAGGATAGGTGTGGTGA	TCCATTTCCAACGCTGAAAC	21	20	42.9	45.0	56.3	56.9	0.0	0.0	3.811180	127054	127173	114-257	1.000000	51.6
ycf1_p4	ycf1	4	AGAAAAGGATAGGTGTGGTGA	CCATTTCCAACGCTGAAACAA	21	21	42.9	42.9	56.3	57.9	0.0	0.0	3.824388	127054	127173	115-256	1.000000	51.5
ycf1_p5	ycf1	5	AGAAAAGGATAGGTGTGGTGAT	CCATTTCCAACGCTGAAACA	22	20	40.9	45.0	56.8	57.2	0.0	0.0	3.998559	127054	127173	114-256	1.000000	50.1