primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCTTTCAGGATCAGTCGTGG CGACGAGAGGGAAGAGATCT 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.053942 4213 5185 1278-1313 1.000000 81.8 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 20 20 55.0 50.0 58.0 57.9 0.0 18.8 0.091715 4213 5185 1380-1416 1.000000 81.5 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 TCAGGATCAGTCGTGGTCTT CGACGAGAGGGAAGAGATCT 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.113475 4213 5185 1274-1309 1.000000 81.4 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 TTCAGGATCAGTCGTGGTCT CGACGAGAGGGAAGAGATCT 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.113475 4213 5185 1275-1310 1.000000 81.4 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 AACCGTAGTAGGGCTCTACC CGACGAGAGGGAAGAGATCT 20 20 55.0 55.0 57.9 58.0 20.4 0.0 0.102640 4213 5185 1441-1476 1.000000 81.2 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 0.0 16.9 0.280333 6316 7103 872-956 1.000000 79.1 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.6 58.1 23.3 16.9 0.491095 6316 7103 882-966 1.000000 77.4 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 57.6 58.1 0.0 16.9 0.498377 6316 7103 849-933 1.000000 77.3 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.4 58.1 23.3 16.9 0.499524 6316 7103 883-967 1.000000 77.3 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 TCATGTCCTTCAAGTCGCAC GTGTCGACTAGAAATGGGGC 20 20 50.0 55.0 58.2 58.3 0.0 17.9 0.551081 6316 7103 934-1018 1.000000 76.8 trnR-UCU-atpA_p1 trnR-UCU-atpA 1 TGGAATGAAAAGCGTCCATTG AGACATTTACCGACGAAGCG 21 20 42.9 50.0 57.4 58.4 5.3 0.0 1.948668 10022 10885 851-1062 1.000000 73.8 trnR-UCU-atpA_p2 trnR-UCU-atpA 2 GGAATGAAAAGCGTCCATTGT AGACATTTACCGACGAAGCG 21 20 42.9 50.0 57.4 58.4 5.3 0.0 1.950350 10022 10885 850-1061 1.000000 73.8 trnR-UCU-atpA_p3 trnR-UCU-atpA 3 GGTTCAAATCCTATTGGACGC AGACATTTACCGACGAAGCG 21 20 47.6 50.0 57.3 58.4 0.0 0.0 2.067175 10022 10885 790-1001 1.000000 73.2 trnR-UCU-atpA_p4 trnR-UCU-atpA 4 TGGATAGGACAGAGGTCTTCT AGACATTTACCGACGAAGCG 21 20 47.6 50.0 56.9 58.4 0.0 0.0 2.425989 10022 10885 825-1036 1.000000 70.1 trnR-UCU-atpA_p5 trnR-UCU-atpA 5 TGGAATGAAAAGCGTCCATT AGACATTTACCGACGAAGCG 20 20 40.0 50.0 55.9 58.4 0.0 0.0 2.453105 10022 10885 851-1062 1.000000 69.8 atpH-atpI_p1 atpH-atpI 1 AATAGAAGCAAGCCCGACAG GCGAATCCATGGAAGGTCAT 20 20 50.0 50.0 58.0 58.0 0.0 20.7 0.056966 14438 15603 1245-1271 1.000000 81.1 atpH-atpI_p2 atpH-atpI 2 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 20 20 45.0 50.0 58.0 58.0 1.4 20.7 0.065515 14438 15603 1211-1237 1.000000 81.0 atpH-atpI_p3 atpH-atpI 3 AATAGAAGCAAGCCCGACAG TTTTTGCAACCTTAGCTGCG 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.162578 14438 15603 1275-1301 1.000000 80.2 atpH-atpI_p4 atpH-atpI 4 ATAACGGAAGCGGCAGAAAT TTTTTGCAACCTTAGCTGCG 20 20 45.0 45.0 58.0 57.9 1.4 0.0 0.171127 14438 15603 1241-1267 1.000000 80.2 atpH-atpI_p5 atpH-atpI 5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 20 20 45.0 50.0 58.2 58.0 1.4 20.7 0.211053 14438 15603 1212-1238 1.000000 79.9 rpoC2_p1 rpoC2 1 GTTGTTGAATCCGTGCCTTG GCCCGCTCTTCCTTTATTGA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.088864 18965 19084 199-205 1.000000 82.5 rpoC2_p2 rpoC2 2 TTGAATCCGTGCCTTGGAAT GCCCGCTCTTCCTTTATTGA 20 20 45.0 50.0 57.8 58.0 0.0 0.0 0.252763 18965 19084 195-201 1.000000 81.2 rpoC2_p3 rpoC2 3 GTTGTTGAATCCGTGCCTTG TTCTTCTATCGAGGAGGCCC 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.344604 18965 19084 215-221 1.000000 80.2 rpoC2_p4 rpoC2 4 TTGAATCCGTGCCTTGGAAT TTCTTCTATCGAGGAGGCCC 20 20 45.0 55.0 57.8 58.3 0.0 0.0 0.508503 18965 19084 211-217 1.000000 79.0 rpoC2_p5 rpoC2 5 AATGGTTGTTGAATCCGTGC GCCCGCTCTTCCTTTATTGA 20 20 45.0 50.0 57.3 58.0 0.0 0.0 0.761511 18965 19084 203-209 1.000000 77.0 petN-psbM_p1 petN-psbM 1 AGTAAATCTCGCTTGGGCTG AAAACAGTCAGCCAAGGTGA 20 20 50.0 45.0 58.0 57.6 0.0 0.0 0.465211 30217 31412 1292-1305 1.000000 78.9 petN-psbM_p2 petN-psbM 2 AGTATGGGGAAGGAGTGGAC AAAACAGTCAGCCAAGGTGA 20 20 55.0 45.0 58.1 57.6 0.0 0.0 0.554432 30217 31412 1235-1248 1.000000 78.3 petN-psbM_p3 petN-psbM 3 GTATGGGGAAGGAGTGGACT AAAACAGTCAGCCAAGGTGA 20 20 55.0 45.0 58.1 57.6 0.0 0.0 0.554432 30217 31412 1234-1247 1.000000 78.3 petN-psbM_p4 petN-psbM 4 TATGGGGAAGGAGTGGACTC AAAACAGTCAGCCAAGGTGA 20 20 55.0 45.0 57.8 57.6 0.0 0.0 0.611620 30217 31412 1233-1246 1.000000 77.8 petN-psbM_p5 petN-psbM 5 GGGGAAGGAGTGGACTCTAG AAAACAGTCAGCCAAGGTGA 20 20 60.0 45.0 58.2 57.6 6.7 0.0 0.652301 30217 31412 1230-1243 1.000000 77.5 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 TCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 20 20 45.0 50.0 57.6 58.5 0.0 5.4 0.912704 31518 32758 1397-1437 1.000000 74.0 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 AATCACCTTGGCTGACTGTT TTCAATTGGTCAGAGCACCG 20 20 45.0 50.0 57.3 58.5 0.0 5.4 1.141734 31518 32758 1399-1439 1.000000 72.2 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 ATCACCTTGGCTGACTGTTT TTCAATTGGTCAGAGCACCG 20 20 45.0 50.0 57.3 58.5 0.0 5.4 1.141734 31518 32758 1398-1438 1.000000 72.2 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 TCACCTTGGCTGACTGTTTT GTTCAATTGGTCAGAGCACC 20 20 45.0 50.0 57.6 57.0 0.0 5.4 1.432858 31518 32758 1398-1438 1.000000 69.8 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 ATCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 21 20 42.9 50.0 58.1 58.5 0.0 5.4 1.527884 31518 32758 1398-1438 1.000000 69.1 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 ATTTGGATTCGTCGGGACTG TGGCAATATGTCTACGCTGG 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.263118 32833 33291 570-585 1.000000 80.8 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 ATTTGGATTCGTCGGGACTG GGCAATATGTCTACGCTGGT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.264669 32833 33291 569-584 1.000000 80.8 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 ATTTGGATTCGTCGGGACTG GGGGACGGACTGTAAATTCG 20 20 50.0 55.0 58.0 58.4 0.0 0.0 0.375672 32833 33291 591-606 1.000000 79.8 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 ATTTGGATTCGTCGGGACTG GGGACGGACTGTAAATTCGT 20 20 50.0 50.0 58.0 57.6 0.0 0.0 0.399349 32833 33291 590-605 1.000000 79.6 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 ATTTGGATTCGTCGGGACTG GATTCTTCCTGGGTCGATGC 20 20 50.0 55.0 58.0 58.4 0.0 0.0 0.436628 32833 33291 624-639 1.000000 79.2 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 20 20 55.0 50.0 58.1 58.0 7.6 0.0 0.074361 34108 35660 1585-1717 1.000000 83.9 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.096516 34108 35660 1588-1720 1.000000 83.8 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 20 20 55.0 50.0 58.1 58.0 7.6 5.0 0.097032 34108 35660 1587-1719 1.000000 83.8 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 20 20 55.0 45.0 58.1 58.0 7.6 0.0 0.106399 34108 35660 1575-1707 1.000000 83.7 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 20 20 50.0 50.0 58.1 58.0 0.0 5.0 0.119187 34108 35660 1590-1722 1.000000 83.6 psbZ-trnG-UCC_p1 psbZ-trnG-UCC 1 TTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 57.7 57.8 0.0 1.1 0.446564 38936 39381 583-650 1.000000 81.5 psbZ-trnG-UCC_p2 psbZ-trnG-UCC 2 GGGTTGGTCGGGTAACAAAA CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 58.3 57.8 0.0 1.1 0.479849 38936 39381 569-636 1.000000 81.2 psbZ-trnG-UCC_p3 psbZ-trnG-UCC 3 TTCCGGCACATCATTATGGA CGTCTTCTCCTTGGCAAAGA 20 20 45.0 50.0 57.0 57.8 0.0 1.1 1.188961 38936 39381 539-606 1.000000 75.6 psbZ-trnG-UCC_p4 psbZ-trnG-UCC 4 AGGGTTGGTCGGGTAACAAA CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 59.2 57.8 0.0 1.1 1.324496 38936 39381 570-637 1.000000 74.5 psbZ-trnG-UCC_p5 psbZ-trnG-UCC 5 ATTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 21 20 47.6 50.0 58.2 57.8 0.0 1.1 1.369583 38936 39381 584-651 1.000000 74.1 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 49477 50747 1267-1332 1.000000 83.0 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 49477 50747 1275-1340 1.000000 82.5 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 49477 50747 1281-1346 1.000000 81.1 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 CTAACCTCTGAGCTAAGCGG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.5 58.0 5.5 0.0 0.505133 49477 50747 1259-1324 1.000000 80.1 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATGCGATGCTCTAACCTCTG ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.5 58.0 0.0 0.0 0.527459 49477 50747 1269-1334 1.000000 79.9 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 20 20 50.0 50.0 57.9 58.2 5.2 0.0 0.304202 54218 54922 845-997 1.000000 83.4 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.9 57.8 5.2 0.0 0.336606 54218 54922 781-933 1.000000 83.2 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 GGGGCTAAAACTCCGGAAAT AATGCACGTTGGGTCTTTGA 20 20 50.0 45.0 57.9 58.3 5.2 0.0 0.452969 54218 54922 848-1000 1.000000 82.2 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 GGGGCTAAAACTCCGGAAAT ATGCACGTTGGGTCTTTGAA 20 20 50.0 45.0 57.9 58.3 5.2 0.0 0.452969 54218 54922 847-999 1.000000 82.2 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CTGGTCCTTTGCTAATCGGG GCACGTTGGGTCTTTGAAAC 20 20 55.0 50.0 58.3 58.2 0.0 0.0 0.496460 54218 54922 862-1014 1.000000 81.8 accD-psaI_p1 accD-psaI 1 AGGGCGTTCTGAGTGAGTTA CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.4 58.0 0.0 26.9 0.397961 62270 63082 938-959 1.000000 80.3 accD-psaI_p2 accD-psaI 2 AGGGCGTTCTGAGTGAGTTA AATGGAGGGGAGACTGTTGA 20 20 50.0 50.0 58.4 58.0 0.0 0.0 0.415750 62270 63082 895-916 1.000000 80.3 accD-psaI_p3 accD-psaI 3 AGGGCGTTCTGAGTGAGTTA TTGCCGGAAATACTAAGCCC 20 20 50.0 50.0 58.4 58.0 0.0 5.0 0.417959 62270 63082 929-950 1.000000 80.1 accD-psaI_p4 accD-psaI 4 AGGGCGTTCTGAGTGAGTTA GAGGGGAGACTGTTGAGAGT 20 20 50.0 55.0 58.4 58.1 0.0 0.0 0.439322 62270 63082 891-912 1.000000 80.1 accD-psaI_p5 accD-psaI 5 AGGGCGTTCTGAGTGAGTTA GCACAAAAATGGAGGGGAGA 20 20 50.0 50.0 58.4 58.1 0.0 0.0 0.447602 62270 63082 902-923 1.000000 80.0 petA-psbJ_p1 petA-psbJ 1 TGCTCTTCTTGGCATCTGTT AGCCGGTATTCTTGTGATCG 20 20 45.0 50.0 57.4 57.8 0.0 1.9 0.799074 66553 67562 1151-1182 1.000000 76.8 petA-psbJ_p2 petA-psbJ 2 TTGCTCTTCTTGGCATCTGT AGCCGGTATTCTTGTGATCG 20 20 45.0 50.0 57.4 57.8 0.0 1.9 0.799074 66553 67562 1152-1183 1.000000 76.8 petA-psbJ_p3 petA-psbJ 3 CGTGTCCAAGGTCTTTTGCT AGCCGGTATTCTTGTGATCG 20 20 50.0 50.0 58.7 57.8 0.0 1.9 0.916070 66553 67562 1167-1198 1.000000 75.8 petA-psbJ_p4 petA-psbJ 4 GGTCTTTTGCTCTTCTTGGC AGCCGGTATTCTTGTGATCG 20 20 50.0 50.0 57.3 57.8 0.0 1.9 0.938964 66553 67562 1158-1189 1.000000 75.6 petA-psbJ_p5 petA-psbJ 5 TCCATTACGTGTCCAAGGTC AGCCGGTATTCTTGTGATCG 20 20 50.0 50.0 57.2 57.8 0.0 1.9 0.980015 66553 67562 1174-1205 1.000000 75.3 rps11-rpl36_p1 rps11-rpl36 1 ACGTCTATTTCTACGCGAACC AAAATAAGGGCCTCCGTTCG 21 20 47.6 50.0 58.2 58.3 15.9 6.5 1.463143 83310 83477 321-335 1.000000 70.5 rps11-rpl36_p2 rps11-rpl36 2 CGTCTATTTCTACGCGAACCA AAAATAAGGGCCTCCGTTCG 21 20 47.6 50.0 58.2 58.3 15.9 6.5 1.464322 83310 83477 320-334 1.000000 70.5 rps11-rpl36_p3 rps11-rpl36 3 ACGTCTATTTCTACGCGAACC AATAAGGGCCTCCGTTCGTA 21 20 47.6 50.0 58.2 58.5 15.9 6.5 1.721223 83310 83477 319-333 1.000000 68.5 rps11-rpl36_p4 rps11-rpl36 4 ACGTCTATTTCTACGCGAACC ATAAGGGCCTCCGTTCGTAA 21 20 47.6 50.0 58.2 58.5 15.9 6.5 1.721223 83310 83477 318-332 1.000000 68.5 rps11-rpl36_p5 rps11-rpl36 5 CGTCTATTTCTACGCGAACCA ATAAGGGCCTCCGTTCGTAA 21 20 47.6 50.0 58.2 58.5 15.9 6.5 1.722403 83310 83477 317-331 1.000000 68.5 ndhF-rpl32_p1 ndhF-rpl32 1 TTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 20 20 40.0 45.0 57.0 57.8 0.0 13.1 1.214860 116798 117866 1245-1343 1.000000 72.6 ndhF-rpl32_p2 ndhF-rpl32 2 TTGTTGCCGTTGGAAAAAGT AAGCTTTCAATGCTGCCCTA 20 20 40.0 45.0 57.0 57.8 0.0 0.0 1.261149 116798 117866 1243-1341 1.000000 72.2 ndhF-rpl32_p3 ndhF-rpl32 3 TTGTTGCCGTTGGAAAAAGT GCTGCCCTATATCCCTTCCT 20 20 40.0 55.0 57.0 58.3 0.0 0.0 1.377810 116798 117866 1232-1330 1.000000 71.3 ndhF-rpl32_p4 ndhF-rpl32 4 TTGTTGCCGTTGGAAAAAGT AGCTTTCAATGCTGCCCTAT 20 20 40.0 45.0 57.0 57.6 0.0 0.0 1.489633 116798 117866 1242-1340 1.000000 70.4 ndhF-rpl32_p5 ndhF-rpl32 5 TTTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 21 20 38.1 45.0 57.7 57.8 0.0 13.1 1.495037 116798 117866 1246-1344 1.000000 70.3 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 57.8 58.0 14.0 0.0 0.182194 118026 119357 1444-1507 1.000000 79.0 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 57.8 58.0 14.0 0.0 0.183564 118026 119357 1445-1508 1.000000 79.0 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 57.8 58.0 0.0 0.0 0.228483 118026 119357 1446-1509 1.000000 78.6 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 57.8 58.0 0.0 0.0 0.229854 118026 119357 1447-1510 1.000000 78.6 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 57.8 57.9 14.0 0.0 0.298641 118026 119357 1454-1517 1.000000 78.0