primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbA-trnK-UUU_p1 psbA-trnK-UUU 1 TAAAGACGGTTTTCGGTGCT CTAGTTCCGGGTTCGAATCC 20 20 45.0 55.0 57.8 57.8 0.0 18.0 0.464081 1330 1530 313-322 1.000000 82.8 psbA-trnK-UUU_p2 psbA-trnK-UUU 2 GTAAAGACGGTTTTCGGTGC CTAGTTCCGGGTTCGAATCC 20 20 50.0 55.0 57.7 57.8 0.0 18.0 0.525731 1330 1530 314-323 1.000000 82.3 psbA-trnK-UUU_p3 psbA-trnK-UUU 3 TACAGAAGCGACCCCATAGG CTAGTTCCGGGTTCGAATCC 20 20 55.0 55.0 58.6 57.8 0.0 18.0 0.810474 1330 1530 282-291 1.000000 80.8 psbA-trnK-UUU_p4 psbA-trnK-UUU 4 TTTTCGGTGCTGGTTATCCA CTAGTTCCGGGTTCGAATCC 20 20 45.0 55.0 57.4 57.8 0.0 18.0 0.790894 1330 1530 304-313 1.000000 80.4 psbA-trnK-UUU_p5 psbA-trnK-UUU 5 TAAAGACGGTTTTCGGTGCT ACTCCATCCGACTAGTTCCG 20 20 45.0 55.0 57.8 58.6 0.0 0.0 0.853037 1330 1530 324-333 1.000000 79.5 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 20 20 45.0 50.0 58.0 57.8 0.0 0.0 0.238579 5742 6996 1334-1394 1.000000 84.6 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 20 20 45.0 50.0 58.0 57.8 0.0 0.0 0.238579 5742 6996 1335-1395 1.000000 84.6 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 20 20 45.0 50.0 58.1 57.8 8.1 0.0 0.339697 5742 6996 1345-1405 1.000000 83.8 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CGGATCGTGTCCTTCAAGTC AAATATAGATGGGGCGTGGC 20 20 55.0 50.0 58.4 57.8 7.8 0.0 0.570225 5742 6996 1360-1420 1.000000 81.9 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 20 20 55.0 50.0 58.4 57.8 2.9 0.0 0.570225 5742 6996 1359-1419 1.000000 81.9 trnG-UCC-trnR-UCU_p1 trnG-UCC-trnR-UCU 1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.0 56.9 0.0 0.0 2.081443 9544 9674 205-229 1.000000 70.0 trnG-UCC-trnR-UCU_p2 trnG-UCC-trnR-UCU 2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 58.1 56.9 0.0 0.0 2.110280 9544 9674 208-232 1.000000 69.7 trnG-UCC-trnR-UCU_p3 trnG-UCC-trnR-UCU 3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 20 22 50.0 45.5 58.0 57.9 0.0 0.0 2.164771 9544 9674 211-235 1.000000 69.2 trnG-UCC-trnR-UCU_p4 trnG-UCC-trnR-UCU 4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 20 22 55.0 45.5 58.1 57.9 0.0 0.0 2.193608 9544 9674 214-238 1.000000 69.0 trnG-UCC-trnR-UCU_p5 trnG-UCC-trnR-UCU 5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 57.7 56.9 0.0 0.0 2.385293 9544 9674 209-233 1.000000 67.5 petN-psbM_p1 petN-psbM 1 ATGGGGAAGAAGTGGACTCT TGTAAAAACAGAAAGTCAAAATCA 20 24 50.0 25.0 57.6 53.9 0.0 0.0 8.496408 28850 30006 1131-1223 1.000000 46.5 petN-psbM_p2 petN-psbM 2 TGGGGAAGAAGTGGACTCTA TGTAAAAACAGAAAGTCAAAATCA 20 24 50.0 25.0 57.0 53.9 0.0 0.0 9.159619 28850 30006 1130-1222 1.000000 46.5 petN-psbM_p3 petN-psbM 3 ATGGGGAAGAAGTGGACTCT GTAAAAACAGAAAGTCAAAATCA 20 23 50.0 26.1 57.6 52.1 0.0 0.0 9.208226 28850 30006 1130-1222 1.000000 46.5 petN-psbM_p4 petN-psbM 4 ATGGGGAAGAAGTGGACTCT TGTAAAAACAGAAAGTCAAAATC 20 23 50.0 26.1 57.6 52.1 0.0 0.0 9.208226 28850 30006 1131-1223 1.000000 46.5 petN-psbM_p5 petN-psbM 5 TGGGGAAGAAGTGGACTCTAG TGTAAAAACAGAAAGTCAAAATCA 21 24 52.4 25.0 58.2 53.9 0.0 0.0 9.324109 28850 30006 1130-1222 1.000000 46.5 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 GGCGAAACGAAAAGGGAAAG GAACCGATGACTTACGCCTT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.052253 31242 32043 1359-1395 1.000000 85.9 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 GAATGGCGAAACGAAAAGGG GAACCGATGACTTACGCCTT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.028782 31242 32043 1363-1399 0.888889 81.4 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 GAATGGCGAAACGAAAAGGG TGGTGATTCCAGAGACGACT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.098081 31242 32043 1607-1671 0.888889 81.4 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 GAATGGCGAAACGAAAAGGG GGCGTTACTCTACCGTTGAG 20 20 50.0 55.0 58.0 58.1 0.0 8.3 0.117362 31242 32043 1338-1374 0.444444 61.0 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 CCTGTTCCCTTGTGTTGACA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.057305 31242 32043 1341-1341 0.111111 44.0 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 21 20 47.6 50.0 58.0 58.1 8.2 0.0 1.134847 45271 46096 910-918 1.000000 73.4 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 21 20 47.6 50.0 58.0 58.2 8.2 0.0 1.191833 45271 46096 931-939 1.000000 72.9 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 21 20 47.6 45.0 58.0 57.8 8.2 0.0 1.233148 45271 46096 881-889 1.000000 72.7 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 21 20 47.6 50.0 58.0 58.3 8.2 0.0 1.301418 45271 46096 883-891 1.000000 72.1 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 21 20 47.6 50.0 58.0 58.4 8.2 0.0 1.427808 45271 46096 911-919 1.000000 71.0 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 47535 48306 812-848 1.000000 85.5 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 47535 48306 844-880 1.000000 84.4 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG ATTAAGTCCGCAGCGTCTAC 20 20 55.0 50.0 57.9 57.8 0.0 15.0 0.338047 47535 48306 830-866 1.000000 83.8 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT ATTAAGTCCGCAGCGTCTAC 20 20 50.0 50.0 57.8 57.8 15.5 15.0 0.445800 47535 48306 862-898 1.000000 82.7 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATGCGATGCTCTAACCTCTG ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.5 58.0 0.0 0.0 0.527459 47535 48306 814-850 1.000000 82.4 psbB-psbT_p1 psbB-psbT 1 GGTGCTAGAACCCTGTTCAG ACCTAGAGTCGAGACTAAGAGG 20 22 55.0 50.0 57.9 57.6 9.0 18.5 2.511861 74703 74913 365-377 1.000000 65.7 psbB-psbT_p2 psbB-psbT 2 GGTGCTAGAACCCTGTTCAG CCTAGAGTCGAGACTAAGAGGA 20 22 55.0 50.0 57.9 57.3 9.0 16.5 2.776581 74703 74913 364-376 1.000000 63.6 psbB-psbT_p3 psbB-psbT 3 GGTGCTAGAACCCTGTTCAG CCTAGAGTCGAGACTAAGAGGAA 20 23 55.0 47.8 57.9 58.0 9.0 16.5 3.126643 74703 74913 364-376 1.000000 60.8 psbB-psbT_p4 psbB-psbT 4 GGTGCTAGAACCCTGTTCAG AGAGTCGAGACTAAGAGGAATGT 20 23 55.0 43.5 57.9 58.1 9.0 5.7 3.249925 74703 74913 361-373 1.000000 59.9 psbB-psbT_p5 psbB-psbT 5 GGTGCTAGAACCCTGTTCAG CCTAGAGTCGAGACTAAGAGG 20 21 55.0 52.4 57.9 55.8 9.0 5.7 3.254642 74703 74913 364-376 1.000000 59.8 ycf2-trnL-CAA_p1 ycf2-trnL-CAA 1 TAGCCGGAAAGTTATGTGGC AAATGGTAGACACGCGAGAC 20 20 50.0 50.0 58.0 58.0 5.0 15.9 0.040511 93479 94110 723-2811 1.000000 86.6 ycf2-trnL-CAA_p2 ycf2-trnL-CAA 2 TAGCCGGAAAGTTATGTGGC TGAAATGGTAGACACGCGAG 20 20 50.0 50.0 58.0 58.0 5.0 15.9 0.041881 93479 94110 725-2813 1.000000 86.6 ycf2-trnL-CAA_p3 ycf2-trnL-CAA 3 TAGCCGGAAAGTTATGTGGC GCCTTGGTGGTGAAATGGTA 20 20 50.0 50.0 58.0 58.1 5.0 0.0 0.114808 93479 94110 735-2823 1.000000 86.0 ycf2-trnL-CAA_p4 ycf2-trnL-CAA 4 TAGCCGGAAAGTTATGTGGC TGGTGAAATGGTAGACACGC 20 20 50.0 50.0 58.0 58.2 5.0 0.0 0.229346 93479 94110 728-2816 1.000000 85.1 ycf2-trnL-CAA_p5 ycf2-trnL-CAA 5 TGGCTTTTCCCGGATGAAAT AAATGGTAGACACGCGAGAC 20 20 45.0 50.0 57.8 58.0 0.7 15.9 0.237960 93479 94110 785-2873 1.000000 84.8 trnV-GAC-rrn16_p1 trnV-GAC-rrn16 1 GGAAGTCATCAGTTCGAGCC CCGACTTGCATGTGTTAAGC 20 20 55.0 50.0 58.4 57.8 0.0 10.4 0.601122 100524 102293 325-2287 1.000000 83.9 trnV-GAC-rrn16_p2 trnV-GAC-rrn16 2 TGGAAGTCATCAGTTCGAGC CCGACTTGCATGTGTTAAGC 20 20 50.0 50.0 57.6 57.8 0.0 10.4 0.617592 100524 102293 326-2288 1.000000 83.7 trnV-GAC-rrn16_p3 trnV-GAC-rrn16 3 AAGTCATCAGTTCGAGCCTG CCGACTTGCATGTGTTAAGC 20 20 50.0 50.0 57.6 57.8 7.6 10.4 0.624119 100524 102293 323-2285 1.000000 83.7 trnV-GAC-rrn16_p4 trnV-GAC-rrn16 4 GGAAGTCATCAGTTCGAGCC GCAGGTTCTTACGCGTTACT 20 20 55.0 50.0 58.4 58.3 0.0 20.8 0.647368 100524 102293 378-2340 1.000000 83.3 trnV-GAC-rrn16_p5 trnV-GAC-rrn16 5 TGGAAGTCATCAGTTCGAGC GCAGGTTCTTACGCGTTACT 20 20 50.0 50.0 57.6 58.3 0.0 20.8 0.663838 100524 102293 379-2341 1.000000 83.2 ycf1-ndhF_p1 ycf1-ndhF 1 CCCTGCCCACACTAGAAAAA GTTGGATCCCTCGGAATTCC 20 20 50.0 55.0 57.7 58.0 0.0 15.0 0.317660 112803 112833 1244-1310 1.000000 85.0 ycf1-ndhF_p2 ycf1-ndhF 2 TACGTATCCGCCTAGCTTGT GTTGGATCCCTCGGAATTCC 20 20 50.0 55.0 58.3 58.0 0.0 15.0 0.347352 112803 112833 1297-1363 1.000000 84.4 ycf1-ndhF_p3 ycf1-ndhF 3 CCCTGCCCACACTAGAAAAA TGGATCCCTCGGAATTCCTT 20 20 50.0 50.0 57.7 58.1 0.0 22.1 0.393049 112803 112833 1242-1308 1.000000 84.4 ycf1-ndhF_p4 ycf1-ndhF 4 CCCTGCCCACACTAGAAAAA TTGGATCCCTCGGAATTCCT 20 20 50.0 50.0 57.7 58.1 0.0 22.1 0.393049 112803 112833 1243-1309 1.000000 84.4 ycf1-ndhF_p5 ycf1-ndhF 5 TACGTATCCGCCTAGCTTGT TGGATCCCTCGGAATTCCTT 20 20 50.0 50.0 58.3 58.1 0.0 22.1 0.422742 112803 112833 1295-1361 1.000000 83.9 ndhF_p1 ndhF 1 TTGAGGCTCTAGATACGGGA GGGGGTAGGATCCGGTAAAT 20 20 50.0 55.0 57.0 58.3 16.7 6.0 1.305993 112834 115071 2883-2940 1.000000 73.6 ndhF_p2 ndhF 2 AAATTGGAAGGGCCTCTCTT GGGGGTAGGATCCGGTAAAT 20 20 45.0 55.0 56.7 58.3 6.5 6.0 1.569507 112834 115071 2541-2609 1.000000 71.9 ndhF_p3 ndhF 3 AATTGGAAGGGCCTCTCTTT GGGGGTAGGATCCGGTAAAT 20 20 45.0 55.0 56.7 58.3 6.5 6.0 1.569507 112834 115071 2540-2608 1.000000 71.9 ndhF_p4 ndhF 4 TGAGGCTCTAGATACGGGAT GGGGGTAGGATCCGGTAAAT 20 20 50.0 55.0 56.7 58.3 16.7 6.0 1.539463 112834 115071 2882-2939 1.000000 71.7 ndhF_p5 ndhF 5 CGAGTTTAAATTGGAAGGGCC GGGGGTAGGATCCGGTAAAT 21 20 47.6 55.0 57.5 58.3 0.0 6.0 1.765353 112834 115071 2548-2616 1.000000 70.3 ndhF-rpl32_p1 ndhF-rpl32 1 GTTGCCGACGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 20 24 50.0 29.2 58.0 55.3 20.5 0.0 6.751912 115072 116039 1083-1150 1.000000 47.2 ndhF-rpl32_p2 ndhF-rpl32 2 TGCCGACGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 58.0 55.3 20.5 0.0 6.763201 115072 116039 1081-1148 1.000000 47.2 ndhF-rpl32_p3 ndhF-rpl32 3 TTGCCGACGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 58.0 55.3 20.5 0.0 6.763201 115072 116039 1082-1149 1.000000 47.2 ndhF-rpl32_p4 ndhF-rpl32 4 AAAAGAAGAAGTCCCGCTCC TCCAAATATTTTTACGAATACGCT 20 24 50.0 29.2 57.8 55.3 0.0 0.0 6.906030 115072 116039 1071-1138 1.000000 47.2 ndhF-rpl32_p5 ndhF-rpl32 5 TTTGTTGCCGACGGAAAAAG TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 57.8 55.3 20.5 0.0 6.915793 115072 116039 1086-1153 1.000000 47.2 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 58.3 58.0 0.0 0.0 0.321366 116202 117121 992-1044 1.000000 84.1 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 20 20 50.0 50.0 58.3 58.0 0.0 0.0 0.322736 116202 117121 993-1045 1.000000 84.1 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 GCAGCCTTAAAAGCGTTGTC CTCGCACTGCTTCCTAAGAG 20 20 50.0 55.0 58.3 58.1 0.0 0.0 0.393618 116202 117121 1013-1065 1.000000 83.5 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 GCAGCCTTAAAAGCGTTGTC CTCTCGCACTGCTTCCTAAG 20 20 50.0 55.0 58.3 58.1 0.0 0.0 0.393618 116202 117121 1015-1067 1.000000 83.5 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GCAGCCTTAAAAGCGTTGTC TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 58.3 57.9 0.0 0.0 0.437813 116202 117121 1002-1054 1.000000 83.1 trnL-UAG-ccsA_p1 trnL-UAG-ccsA 1 GTGAAATCGGTAGACACGCT ACAATGGAAACGATCGAAAAGG 20 22 50.0 40.9 58.0 57.6 0.0 24.3 2.427353 117202 117297 227-229 1.000000 64.8 trnL-UAG-ccsA_p2 trnL-UAG-ccsA 2 TGAAATCGGTAGACACGCTG ACAATGGAAACGATCGAAAAGG 20 22 50.0 40.9 58.0 57.6 0.0 24.3 2.428723 117202 117297 226-228 1.000000 64.8 trnL-UAG-ccsA_p3 trnL-UAG-ccsA 3 CTTAGGAAGCAGTGCGAGAG ACAATGGAAACGATCGAAAAGG 20 22 55.0 40.9 58.1 57.6 10.0 24.3 2.499604 117202 117297 204-206 1.000000 64.7 trnL-UAG-ccsA_p4 trnL-UAG-ccsA 4 CTCTTAGGAAGCAGTGCGAG ACAATGGAAACGATCGAAAAGG 20 22 55.0 40.9 58.1 57.6 10.0 24.3 2.499604 117202 117297 206-208 1.000000 64.6 trnL-UAG-ccsA_p5 trnL-UAG-ccsA 5 TGAATTCGAACAAGCCGCTA ACAATGGAAACGATCGAAAAGG 20 22 45.0 40.9 57.9 57.6 0.0 24.3 2.507023 117202 117297 249-251 1.000000 63.8 ccsA_p1 ccsA 1 AACCGACAAAAGCCTGGAAT TGGACCGCGAGAGTTATTTG 20 20 45.0 50.0 58.0 58.0 0.0 8.2 0.009206 117298 118272 1895-1942 1.000000 84.2 ccsA_p2 ccsA 2 ACCGACAAAAGCCTGGAATT TGGACCGCGAGAGTTATTTG 20 20 45.0 50.0 58.0 58.0 0.0 8.2 0.009206 117298 118272 1894-1941 1.000000 84.2 ccsA_p3 ccsA 3 AACCGACAAAAGCCTGGAAT GGACCGCGAGAGTTATTTGT 20 20 45.0 50.0 58.0 58.0 0.0 8.2 0.010594 117298 118272 1894-1941 1.000000 84.2 ccsA_p4 ccsA 4 ACCGACAAAAGCCTGGAATT GGACCGCGAGAGTTATTTGT 20 20 45.0 50.0 58.0 58.0 0.0 8.2 0.010594 117298 118272 1893-1940 1.000000 84.2 ccsA_p5 ccsA 5 GTGAAATCGGTAGACACGCT TGGACCGCGAGAGTTATTTG 20 20 50.0 50.0 58.0 58.0 0.0 8.2 0.012413 117298 118272 1498-1522 1.000000 82.9 ccsA-ndhD_p1 ccsA-ndhD 1 GGGCTACATAGTTATGGTTCA CAGTTGACAAGGTCGAGGTT 21 20 42.9 50.0 54.8 57.8 4.3 0.0 4.444365 118273 118501 312-336 1.000000 51.7 ccsA-ndhD_p2 ccsA-ndhD 2 GGGCTACATAGTTATGGTTCA AGTTGACAAGGTCGAGGTTC 21 20 42.9 50.0 54.8 57.5 4.3 0.0 4.731913 118273 118501 311-335 1.000000 49.4 ccsA-ndhD_p3 ccsA-ndhD 3 GGGCTACATAGTTATGGTTCA GTTGACAAGGTCGAGGTTCT 21 20 42.9 50.0 54.8 57.5 4.3 0.0 4.731913 118273 118501 310-334 1.000000 49.4 ccsA-ndhD_p4 ccsA-ndhD 4 GGGCTACATAGTTATGGTTCA TCAGTTGACAAGGTCGAGGT 21 20 42.9 50.0 54.8 58.6 4.3 0.0 4.793867 118273 118501 313-337 1.000000 48.9 ccsA-ndhD_p5 ccsA-ndhD 5 GGGCTACATAGTTATGGTTCAT CAGTTGACAAGGTCGAGGTT 22 20 40.9 50.0 55.4 57.8 4.3 0.0 4.863418 118273 118501 312-336 1.000000 48.3