primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	57.8	57.9	0.0	0.0	0.376342	1545	1763	415-422	1.000000	80.6
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	57.8	57.8	0.0	18.0	0.464081	1545	1763	330-337	1.000000	80.6
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.437992	1545	1763	416-423	1.000000	80.1
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	20	20	50.0	55.0	57.7	57.8	0.0	18.0	0.525731	1545	1763	331-338	1.000000	80.1
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TAAAGACGGTTTTCGGTGCT	TTTGGGGAGGGGTTTTTACC	20	20	45.0	50.0	57.8	57.6	0.0	0.0	0.645784	1545	1763	382-389	1.000000	78.8
rps16-trnQ-TTG_p1	rps16-trnQ-TTG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6351	8115	1263-1838	1.000000	85.0
rps16-trnQ-TTG_p2	rps16-trnQ-TTG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6351	8115	1278-1853	1.000000	83.1
rps16-trnQ-TTG_p3	rps16-trnQ-TTG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6351	8115	1277-1852	1.000000	83.1
rps16-trnQ-TTG_p4	rps16-trnQ-TTG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6351	8115	1334-1909	1.000000	82.0
rps16-trnQ-TTG_p5	rps16-trnQ-TTG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6351	8115	1267-1842	1.000000	80.3
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	ATCTAATGATCCGGGGCGTA	CGCGGTGGAAGAACAGATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	9481	11155	2354-2389	1.000000	82.8
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	TATCTAATGATCCGGGGCGT	CGCGGTGGAAGAACAGATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	9481	11155	2355-2390	1.000000	82.8
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	ATTAGCAATCCGCCGCTTTA	CGCGGTGGAAGAACAGATAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.042789	9481	11155	2127-2155	1.000000	82.7
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	ATTAGCAATCCGCCGCTTTA	CGTTTCCAGAGTGGGATCTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.053942	9481	11155	2325-2353	1.000000	82.5
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	ATCTAATGATCCGGGGCGTA	CGTTTCCAGAGTGGGATCTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.082714	9481	11155	2552-2587	1.000000	82.5
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGTCAAGCCCTGATCAATGA	CTTACCACTCGGCCATATCG	20	20	50.0	55.0	58.0	57.9	0.0	2.2	0.094413	28215	29489	1361-1407	1.000000	82.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110548	28215	29489	1401-1447	1.000000	82.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	20	20	50.0	55.0	57.8	57.9	0.0	2.2	0.240678	28215	29489	1289-1335	1.000000	81.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256813	28215	29489	1329-1375	1.000000	81.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CGTCAAGCCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.0	58.4	0.0	14.6	0.383911	28215	29489	1383-1429	1.000000	80.3
trnE-TTC-trnT-GGT_p1	trnE-TTC-trnT-GGT	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	32872	33693	902-934	1.000000	84.2
trnE-TTC-trnT-GGT_p2	trnE-TTC-trnT-GGT	2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32872	33693	939-971	1.000000	84.1
trnE-TTC-trnT-GGT_p3	trnE-TTC-trnT-GGT	3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	32872	33693	867-899	1.000000	83.7
trnE-TTC-trnT-GGT_p4	trnE-TTC-trnT-GGT	4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.262716	32872	33693	904-936	1.000000	83.5
trnE-TTC-trnT-GGT_p5	trnE-TTC-trnT-GGT	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32872	33693	880-912	1.000000	83.5
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	44082	44803	886-916	1.000000	80.7
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TATGGCGCTTACTCCTGGTA	20	20	50.0	50.0	57.9	57.9	4.1	7.6	0.191550	44082	44803	858-888	1.000000	80.5
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	44082	44803	888-918	1.000000	79.9
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	44082	44803	815-845	1.000000	79.9
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	44082	44803	819-849	1.000000	79.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTGGGAA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.3	58.1	0.0	0.0	1.808440	46780	47638	1022-1031	1.000000	67.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTGGGA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.3	58.1	0.0	0.0	1.808440	46780	47638	1023-1032	1.000000	67.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTGGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.3	58.2	0.0	0.0	1.865425	46780	47638	1043-1052	1.000000	66.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTGGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.3	58.2	0.0	0.0	1.865425	46780	47638	1044-1053	1.000000	66.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTGGGAA	TTCCAATGCTACGCCTTCAA	20	20	45.0	45.0	56.3	57.8	0.0	0.0	1.906741	46780	47638	993-1002	1.000000	66.4
ndhC-trnM-CAT_p1	ndhC-trnM-CAT	1	CTCCGGAAATGAAAAATGCCA	CCCGCCGTATGAAAGCAATA	21	20	42.9	50.0	57.4	58.4	5.2	0.0	2.002521	52936	54783	1888-1993	1.000000	70.1
ndhC-trnM-CAT_p2	ndhC-trnM-CAT	2	ACTCCGGAAATGAAAAATGCC	CCCGCCGTATGAAAGCAATA	21	20	42.9	50.0	57.4	58.4	5.2	0.0	2.004224	52936	54783	1889-1994	1.000000	70.1
ndhC-trnM-CAT_p3	ndhC-trnM-CAT	3	TCCGGAAATGAAAAATGCCA	CCCGCCGTATGAAAGCAATA	20	20	40.0	50.0	56.2	58.4	0.0	0.0	2.212602	52936	54783	1887-1992	1.000000	68.4
ndhC-trnM-CAT_p4	ndhC-trnM-CAT	4	CTCCGGAAATGAAAAATGCCA	CCGCCGTATGAAAGCAATAC	21	20	42.9	50.0	57.4	57.3	5.2	0.0	2.254474	52936	54783	1887-1992	1.000000	68.1
ndhC-trnM-CAT_p5	ndhC-trnM-CAT	5	ACTCCGGAAATGAAAAATGCC	CCGCCGTATGAAAGCAATAC	21	20	42.9	50.0	57.4	57.3	5.2	0.0	2.256177	52936	54783	1888-1993	1.000000	68.1
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.051764	67161	68313	1100-1317	1.000000	85.9
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.057305	67161	68313	1102-1319	1.000000	85.8
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	67161	68313	1109-1326	1.000000	84.9
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	67161	68313	1023-1240	0.928571	82.3
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	67161	68313	1025-1242	0.928571	82.2
rpl20-rps12_p1	rpl20-rps12	1	TCTACGTCGCCGAGCTATAT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.821234	71365	72139	846-848	1.000000	75.3
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	71365	72139	893-895	1.000000	75.0
rpl20-rps12_p3	rpl20-rps12	3	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	71365	72139	892-894	1.000000	75.0
rpl20-rps12_p4	rpl20-rps12	4	TTCTACGTCGCCGAGCTATA	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.1	58.7	0.0	9.8	0.861060	71365	72139	847-849	1.000000	75.0
rpl20-rps12_p5	rpl20-rps12	5	TTTTTGTTCTACGTCGCCGA	CAGCGTCGAGGAACATGTAC	20	20	45.0	55.0	58.1	58.7	0.0	9.8	0.880457	71365	72139	853-855	1.000000	74.8
petB_p1	petB	1	GTGTATTTCTAGCCCAGCCC	ACTTTCTGGGTCGTCTCAGA	20	20	55.0	50.0	58.0	58.0	0.0	8.2	0.034497	77337	78768	2172-2173	0.857143	75.5
petB_p2	petB	2	GTGTATTTCTAGCCCAGCCC	TTCAAGCGAACCAGCCTATC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.055471	77337	78768	1834-1835	0.857143	75.4
petB_p3	petB	3	GTGTATTTCTAGCCCAGCCC	CTTCAAGCGAACCAGCCTAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.061134	77337	78768	1835-1836	0.857143	75.4
petB_p4	petB	4	TCCTTTGATGGGTGTTGCAA	ACTTTCTGGGTCGTCTCAGA	20	20	45.0	50.0	57.9	58.0	0.0	8.2	0.082371	77337	78768	2331-2331	0.071429	42.2
petB_p5	petB	5	TCCTTTGATGGGTGTTGCAA	TTCAAGCGAACCAGCCTATC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.103345	77337	78768	1993-1993	0.071429	42.0
rpl16-rps3_p1	rpl16-rps3	1	GTTCAAGTGCCTGAAGAGCA	CCTGCAAACCATTCGAGCTA	20	20	50.0	50.0	58.4	58.3	0.0	0.0	0.664420	84033	85142	1283-1346	1.000000	76.7
rpl16-rps3_p2	rpl16-rps3	2	AGGTTCAAGTGCCTGAAGAG	CCTGCAAACCATTCGAGCTA	20	20	50.0	50.0	57.4	58.3	0.0	0.0	0.820081	84033	85142	1285-1348	1.000000	75.4
rpl16-rps3_p3	rpl16-rps3	3	TTCAAGTGCCTGAAGAGCAT	CCTGCAAACCATTCGAGCTA	20	20	45.0	50.0	57.4	58.3	0.0	0.0	0.836167	84033	85142	1282-1345	1.000000	75.3
rpl16-rps3_p4	rpl16-rps3	4	TGTGATCCAAGCAGGTTCAA	CCTGCAAACCATTCGAGCTA	20	20	45.0	50.0	57.3	58.3	9.7	0.0	0.917251	84033	85142	1297-1360	1.000000	74.6
rpl16-rps3_p5	rpl16-rps3	5	ATCCAAGCAGGTTCAAGTGC	CCTGCAAACCATTCGAGCTA	20	20	50.0	50.0	58.7	58.3	0.0	0.0	1.010911	84033	85142	1293-1356	1.000000	73.9
ndhF_p1	ndhF	1	GATGGAATCGCCCATTACGA	CGAACAGGTTGGTCATCTCA	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.619491	112563	114788	2785-2820	0.857143	71.9
ndhF_p2	ndhF	2	GATGGAATCGCCCATTACGA	ACGAACAGGTTGGTCATCTC	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.621184	112563	114788	2786-2821	0.857143	71.9
ndhF_p3	ndhF	3	ACATGCCGAAGTGATGGAAA	CGAACAGGTTGGTCATCTCA	20	20	45.0	50.0	57.8	57.5	0.0	0.0	0.654500	112563	114788	2702-2737	0.857143	71.6
ndhF_p4	ndhF	4	AGGATGTCCTTGCCCATACT	CGAACAGGTTGGTCATCTCA	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.556811	112563	114788	2621-2656	0.714286	66.1
ndhF_p5	ndhF	5	AGGATGTCCTTGCCCATACT	ACGAACAGGTTGGTCATCTC	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.558503	112563	114788	2622-2657	0.714286	66.1
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	20	20	50.0	50.0	58.0	58.2	17.5	16.6	0.199227	114789	115986	1362-1383	1.000000	82.3
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	17.5	16.6	0.210516	114789	115986	1360-1381	1.000000	82.2
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	17.5	16.6	0.210516	114789	115986	1361-1382	1.000000	82.2
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	20	20	50.0	50.0	58.0	58.3	17.5	0.0	0.345950	114789	115986	1373-1394	1.000000	81.1
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	GACAACGCTTTTAAGGCTGC	20	20	45.0	50.0	58.0	58.3	17.5	0.0	0.357239	114789	115986	1371-1392	1.000000	81.0
rpl32-trnL-TAG_p1	rpl32-trnL-TAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	116152	116970	939-951	1.000000	82.2
rpl32-trnL-TAG_p2	rpl32-trnL-TAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	116152	116970	940-952	1.000000	82.1
rpl32-trnL-TAG_p3	rpl32-trnL-TAG	3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	116152	116970	949-961	1.000000	81.2
rpl32-trnL-TAG_p4	rpl32-trnL-TAG	4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.321366	116152	116970	928-940	1.000000	81.0
rpl32-trnL-TAG_p5	rpl32-trnL-TAG	5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.322736	116152	116970	929-941	1.000000	81.0
ycf1_p1	ycf1	1	ATCCGCGGCTTTTCCTTTAT	AGGATGTCCTTGCCCATACT	20	20	45.0	50.0	57.9	58.1	23.5	0.0	0.153920	130092	130211	998-1091	0.500000	66.6
ycf1_p2	ycf1	2	GTTTGCGGAAAAGGAACGAG	ACATGCCGAAGTGATGGAAA	20	20	50.0	45.0	58.0	57.8	2.8	0.0	0.231241	130092	130211	1214-1214	0.214286	49.6
ycf1_p3	ycf1	3	GTTTGCGGAAAAGGAACGAG	AGGATGTCCTTGCCCATACT	20	20	50.0	50.0	58.0	58.1	2.8	0.0	0.133551	130092	130211	1133-1133	0.071429	41.8
ycf1_p4	ycf1	4	TTTGCGGAAAAGGAACGAGA	AGGATGTCCTTGCCCATACT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.144841	130092	130211	1132-1132	0.071429	41.7
ycf1_p5	ycf1	5	AGAGGTCCGCAACTTAGACT	AGGATGTCCTTGCCCATACT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.175029	130092	130211	1425-1425	0.071429	41.5