primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	79	278	305-325	1.000000	88.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	79	278	319-339	1.000000	88.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	79	278	318-338	1.000000	88.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	79	278	320-340	1.000000	88.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	79	278	306-326	1.000000	87.8
psbA_p1	psbA	1	GATCCACTTGGCTACATCCG	GAAAGCCGTGTGCAATGAAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.261660	279	1337	1634-1655	1.000000	82.5
psbA_p2	psbA	2	CAATCCACTGCCTTGATCCA	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.266021	279	1337	1648-1669	1.000000	82.5
psbA_p3	psbA	3	AATCCACTGCCTTGATCCAC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.267529	279	1337	1647-1668	1.000000	82.5
psbA_p4	psbA	4	ACAATCCACTGCCTTGATCC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.267529	279	1337	1649-1670	1.000000	82.4
psbA_p5	psbA	5	CATCCGCCCCTCTACTATGA	GAAAGCCGTGTGCAATGAAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.208009	279	1337	1620-1636	0.875000	77.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GTCGGGGTCTTCCAAACTTT	TCTTGAAAGGGGTGCTCAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041687	4147	4951	986-1023	1.000000	87.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TCTTGAAAGGGGTGCTCAAC	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.187163	4147	4951	904-941	1.000000	86.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	TCTTGAAAGGGGTGCTCAAC	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.187163	4147	4951	903-940	1.000000	86.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCGGGGTCTTCCAAACTTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.0	58.2	0.0	1.3	0.213260	4147	4951	976-1013	1.000000	85.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCTAGCCGCACTTAAAAGCC	TCTTGAAAGGGGTGCTCAAC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.284581	4147	4951	918-955	1.000000	85.6
rps16_p1	rps16	1	GATACGAACCGAGCAGGAAG	AAACATGCTGCTTATTGGCG	20	20	55.0	45.0	58.2	57.7	5.2	0.0	0.441251	4952	6075	1720-1720	0.062500	39.0
rps16_p2	rps16	2	TAGATACGAACCGAGCAGGA	AAACATGCTGCTTATTGGCG	20	20	50.0	45.0	57.7	57.7	5.2	0.0	0.619646	4952	6075	1722-1722	0.062500	37.5
rps16_p3	rps16	3	GATACGAACCGAGCAGGAAG	AACATGCTGCTTATTGGCGA	20	20	55.0	45.0	58.2	58.5	5.2	0.0	0.695934	4952	6075	1719-1719	0.062500	36.9
rps16_p4	rps16	4	GATACGAACCGAGCAGGAAG	ACATGCTGCTTATTGGCGAA	20	20	55.0	45.0	58.2	58.5	5.2	0.0	0.695934	4952	6075	1718-1718	0.062500	36.9
rps16_p5	rps16	5	AGATACGAACCGAGCAGGAA	AAACATGCTGCTTATTGGCG	20	20	50.0	45.0	58.5	57.7	5.2	0.0	0.811582	4952	6075	1721-1721	0.062500	36.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	TCGGAGGTTCGAATCCTTTC	20	20	50.0	50.0	57.9	57.3	7.8	16.9	0.747759	6076	7139	1136-1160	1.000000	81.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	TAAGGCAACGGGTTTTGGTC	20	20	50.0	50.0	57.9	58.7	7.8	0.0	0.759779	6076	7139	1163-1187	1.000000	81.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6076	7139	1119-1143	1.000000	81.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6076	7139	1146-1170	1.000000	81.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAACGGATCGTGTCCTTCAA	TCGGAGGTTCGAATCCTTTC	20	20	50.0	50.0	58.2	57.3	7.8	16.9	0.876718	6076	7139	1137-1161	1.000000	80.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8459	9230	833-855	1.000000	61.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8459	9230	832-854	1.000000	61.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8459	9230	835-857	1.000000	60.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8459	9230	834-856	1.000000	60.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	8459	9230	887-909	1.000000	59.7
rps2-rpoC2_p1	rps2-rpoC2	1	GCTTCCAGCATCTCTTCCAA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.367500	16535	16734	358-376	1.000000	85.8
rps2-rpoC2_p2	rps2-rpoC2	2	AATGAACTCCTGCTTCCAGC	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.416119	16535	16734	369-387	1.000000	85.2
rps2-rpoC2_p3	rps2-rpoC2	3	CCAGCATCTCTTCCAAGGTG	TTCGAGGGGGAAATGAGAGA	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.499990	16535	16734	354-372	1.000000	84.6
rps2-rpoC2_p4	rps2-rpoC2	4	GCTTCCAGCATCTCTTCCAA	TCGAGGGGGAAATGAGAGAT	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.598673	16535	16734	357-375	1.000000	84.0
rps2-rpoC2_p5	rps2-rpoC2	5	TCCAGCATCTCTTCCAAGGT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.586236	16535	16734	355-373	1.000000	83.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31245	31928	820-837	1.000000	85.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	31245	31928	799-816	1.000000	85.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	31245	31928	796-813	1.000000	84.9
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	31245	31928	817-834	1.000000	84.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CTCCGTGAAAGAGAGGTGTC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.6	58.0	0.0	0.0	0.361687	31245	31928	777-794	1.000000	84.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	32001	33322	1475-1511	1.000000	82.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	32001	33322	1478-1514	1.000000	82.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	32001	33322	1465-1501	1.000000	81.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	32001	33322	1468-1504	1.000000	81.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CCCTTCGTAACCAGTCATCC	20	20	55.0	55.0	58.1	57.7	7.6	0.0	0.375192	32001	33322	1449-1485	1.000000	80.7
psaA-ycf3_p1	psaA-ycf3	1	GCATCAGCATGTAGGTTCCA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.083644	42247	42972	956-982	1.000000	86.7
psaA-ycf3_p2	psaA-ycf3	2	GCATCAGCATGTAGGTTCCA	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.0	58.0	0.0	18.7	0.087759	42247	42972	938-964	1.000000	86.7
psaA-ycf3_p3	psaA-ycf3	3	GCATCAGCATGTAGGTTCCA	TGGTAACGGGACACCAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.072594	42247	42972	1105-1131	1.000000	86.3
psaA-ycf3_p4	psaA-ycf3	4	GTTATTCCAGATGCTCGCCA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.087399	42247	42972	1250-1276	1.000000	85.3
psaA-ycf3_p5	psaA-ycf3	5	GTTATTCCAGATGCTCGCCA	TGGTAACGGGACACCAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.076349	42247	42972	1399-1425	1.000000	84.9
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.0	58.5	4.9	0.0	0.574654	69773	70535	864-932	1.000000	78.8
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	69773	70535	919-987	1.000000	78.0
rpl20-rps12_p3	rpl20-rps12	3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	69773	70535	918-986	1.000000	78.0
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.0	58.7	4.9	9.8	0.764938	69773	70535	827-895	1.000000	77.4
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	69773	70535	881-949	1.000000	76.6
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	21	20	47.6	50.0	57.6	57.8	0.0	0.0	1.637541	75094	75158	117-118	1.000000	73.0
psbT-psbN_p2	psbT-psbN	2	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	21	20	47.6	50.0	57.6	57.8	0.0	0.0	1.637541	75094	75158	118-119	1.000000	73.0
psbT-psbN_p3	psbT-psbN	3	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	21	20	47.6	55.0	57.6	58.3	0.0	0.0	1.727841	75094	75158	120-121	1.000000	72.1
psbT-psbN_p4	psbT-psbN	4	CGAGAACCACCTAAAGTTCCA	ATTCGAGGAACATGGGGACT	21	20	47.6	50.0	57.6	58.4	0.0	0.0	1.811536	75094	75158	119-120	1.000000	71.5
psbT-psbN_p5	psbT-psbN	5	TCGAGAACCACCTAAAGTTCC	TCGAGGAACATGGGGACTAA	21	20	47.6	50.0	57.3	57.8	0.0	0.0	1.914047	75094	75158	118-119	1.000000	70.7
petD_p1	petD	1	TCTCCGAAGAGAAAATGGAT	TCGCTTTGAAACGATTATTCCC	20	22	40.0	40.9	53.7	57.4	0.0	0.0	6.861999	77291	78529	1283-1321	1.000000	45.1
petD_p2	petD	2	TCTCCGAAGAGAAAATGGAT	CGCTTTGAAACGATTATTCCCT	20	22	40.0	40.9	53.7	57.4	0.0	0.0	6.868489	77291	78529	1282-1320	1.000000	45.1
petD_p3	petD	3	TCTCCGAAGAGAAAATGGATT	TCGCTTTGAAACGATTATTCCC	21	22	38.1	40.9	54.6	57.4	0.0	0.0	6.974120	77291	78529	1283-1321	1.000000	45.1
petD_p4	petD	4	TCTCCGAAGAGAAAATGGATT	CGCTTTGAAACGATTATTCCCT	21	22	38.1	40.9	54.6	57.4	0.0	0.0	6.980610	77291	78529	1282-1320	1.000000	45.1
petD_p5	petD	5	TCTCCGAAGAGAAAATGGAT	TTCCCTAGATACACATGCCT	20	20	40.0	45.0	53.7	55.3	0.0	0.0	6.955731	77291	78529	1266-1304	0.937500	42.1
ndhF-rpl32_p1	ndhF-rpl32	1	AAAAGCCCACATACGACGAA	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.211238	112264	112986	852-978	1.000000	88.4
ndhF-rpl32_p2	ndhF-rpl32	2	GCAAGAGGTCGTGTAAACCA	ACGATGCACAATACCCCTTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.164689	112264	112986	1238-1364	1.000000	87.7
ndhF-rpl32_p3	ndhF-rpl32	3	AATAGAGCAGCACACAACGT	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.208219	112264	112986	1424-1550	1.000000	87.0
ndhF-rpl32_p4	ndhF-rpl32	4	GCAGCACACAACGTAACAAA	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.219812	112264	112986	1418-1544	1.000000	86.9
ndhF-rpl32_p5	ndhF-rpl32	5	GCCCACATACGACGAAGATT	ACGATGCACAATACCCCTTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.102454	112264	112986	848-955	0.687500	75.5
ccsA-ndhD_p1	ccsA-ndhD	1	AGCAATTGTGGCGTTTATTGG	ACCCGTACTAGGTATTGGTATGT	21	23	42.9	43.5	58.0	58.2	7.4	2.8	4.233745	115168	115406	432-447	1.000000	55.7
ccsA-ndhD_p2	ccsA-ndhD	2	GCAATTGTGGCGTTTATTGGA	ACCCGTACTAGGTATTGGTATGT	21	23	42.9	43.5	58.0	58.2	7.4	2.8	4.238938	115168	115406	431-446	1.000000	55.7
ccsA-ndhD_p3	ccsA-ndhD	3	AATTCAGCAATTGTGGCGTT	ACCCGTACTAGGTATTGGTATGT	20	23	40.0	43.5	57.2	58.2	7.4	2.8	4.010089	115168	115406	450-450	0.062500	10.4
ccsA-ndhD_p4	ccsA-ndhD	4	ATTCAGCAATTGTGGCGTTT	ACCCGTACTAGGTATTGGTATGT	20	23	40.0	43.5	57.2	58.2	7.4	2.8	4.010089	115168	115406	449-449	0.062500	10.4
ccsA-ndhD_p5	ccsA-ndhD	5	AATTCAGCAATTGTGGCGTTT	ACCCGTACTAGGTATTGGTATGT	21	23	38.1	43.5	57.9	58.2	7.4	2.8	4.302406	115168	115406	450-450	0.062500	8.1
ndhD-psaC_p1	ndhD-psaC	1	TTTGCCCACTGTACATTGCT	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	58.0	58.0	7.7	22.2	0.011414	116928	117028	652-658	1.000000	85.3
ndhD-psaC_p2	ndhD-psaC	2	TTTGCCCACTGTACATTGCT	GACCGAAGACTGTGTTGGTT	20	20	45.0	50.0	58.0	58.1	7.7	11.2	0.056579	116928	117028	604-610	1.000000	85.2
ndhD-psaC_p3	ndhD-psaC	3	TTTGCCCACTGTACATTGCT	CCGACCGATTTCTTGAGTGT	20	20	45.0	50.0	58.0	57.9	7.7	0.0	0.086330	116928	117028	561-567	1.000000	85.2
ndhD-psaC_p4	ndhD-psaC	4	TTTGCCCACTGTACATTGCT	AAATGATACCCTGGGACGGA	20	20	45.0	50.0	58.0	58.1	7.7	22.2	0.118247	116928	117028	656-662	1.000000	84.4
ndhD-psaC_p5	ndhD-psaC	5	TTTGCCCACTGTACATTGCT	AATGATACCCTGGGACGGAT	20	20	45.0	50.0	58.0	57.9	7.7	22.2	0.124447	116928	117028	655-661	1.000000	84.3
ycf1_p1	ycf1	1	CCTCAGACCATTCAGGAAGT	TGGGTAGGAAAAAGGAAAGT	20	20	50.0	40.0	56.5	54.0	0.0	0.0	5.437042	126692	126811	268-268	0.937500	45.3
ycf1_p2	ycf1	2	TCCTCAGACCATTCAGGAAGT	TGGGTAGGAAAAAGGAAAGT	21	20	47.6	40.0	58.1	54.0	0.0	0.0	5.066762	126692	126811	269-269	0.937500	45.2
ycf1_p3	ycf1	3	TCCTCAGACCATTCAGGAAGT	TTGGGTAGGAAAAAGGAAAGT	21	21	47.6	38.1	58.1	54.9	0.0	0.0	5.180105	126692	126811	270-270	0.937500	45.2
ycf1_p4	ycf1	4	CCTCAGACCATTCAGGAAGT	TTGGGTAGGAAAAAGGAAAGT	20	21	50.0	38.1	56.5	54.9	0.0	0.0	5.550384	126692	126811	269-269	0.937500	45.2
ycf1_p5	ycf1	5	TCCTCAGACCATTCAGGAAGT	ATTGGGTAGGAAAAAGGAAAGT	21	22	47.6	36.4	58.1	55.5	0.0	0.0	5.594216	126692	126811	271-271	0.937500	45.2