primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	TTGTTTCAGCTCGGTGGAAA	GCCCAATTCGCAGAGTTAGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.069737	8422	8580	890-1125	1.000000	87.1
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	TTGTTTCAGCTCGGTGGAAA	TCTCCAGAGTAGGATCTGCG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.071296	8422	8580	961-1196	1.000000	87.1
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TTGTTTCAGCTCGGTGGAAA	CGGTGGAAGAACAGGTAGTG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.125847	8422	8580	763-998	1.000000	87.0
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TTGTTTCAGCTCGGTGGAAA	GGTCGTGAAGCTTATCCAGG	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.104236	8422	8580	1174-1440	1.000000	86.3
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TTGTTTCAGCTCGGTGGAAA	AATTCTGGAATCCGACCTGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.089684	8422	8580	1198-1236	0.857143	76.2
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	18998	19921	878-987	0.857143	83.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	18998	19921	872-981	0.857143	81.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTATCGTTTTGGCGGCATG	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	57.7	58.0	14.3	0.0	0.276319	18998	19921	931-1040	0.857143	81.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	18998	19921	896-1005	0.857143	80.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	18998	19921	932-1041	0.857143	80.1
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	TGAGCTGCTAGTCGTAGAAT	20	20	50.0	45.0	58.1	55.8	0.0	0.2	2.301255	30173	30218	126-388	0.428571	42.5
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	AGGCTTTTGTTTACCGAGGG	AATGAGCTGCTAGTCGTAGA	20	20	50.0	45.0	58.1	55.8	0.0	0.2	2.301255	30173	30218	128-390	0.428571	42.4
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	AGGCTTTTGTTTACCGAGGG	ATGAGCTGCTAGTCGTAGAA	20	20	50.0	45.0	58.1	55.8	0.0	0.2	2.301255	30173	30218	127-389	0.428571	42.4
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	AGGCTTTTGTTTACCGAGGG	TGACTCTTCACTCATCCCAA	20	20	50.0	45.0	58.1	55.8	0.0	0.0	2.312910	30173	30218	202-464	0.428571	41.6
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	AGGCTTTTGTTTACCGAGGG	TTGACTCTTCACTCATCCCA	20	20	50.0	45.0	58.1	55.8	0.0	0.0	2.312910	30173	30218	203-465	0.428571	41.6
petA-psbJ_p1	petA-psbJ	1	CCCGTGTCCAAGGTCTTTTT	TGGCTTATAGGCACTGTAGC	20	20	50.0	50.0	58.3	57.1	0.0	9.3	1.223125	46833	47227	518-601	1.000000	79.2
petA-psbJ_p2	petA-psbJ	2	CCCGTGTCCAAGGTCTTTTT	AGGCACTGTAGCTGGTATTC	20	20	50.0	50.0	58.3	57.0	0.0	0.0	1.302503	46833	47227	510-593	1.000000	78.6
petA-psbJ_p3	petA-psbJ	3	CCCGTGTCCAAGGTCTTTTT	GGCACTGTAGCTGGTATTCT	20	20	50.0	50.0	58.3	57.0	0.0	0.0	1.302503	46833	47227	509-592	1.000000	78.6
petA-psbJ_p4	petA-psbJ	4	CCCGTGTCCAAGGTCTTTTT	ACTCTGGATTGGGTTCATCC	20	20	50.0	50.0	58.3	56.9	0.0	0.0	1.422924	46833	47227	451-534	1.000000	78.3
petA-psbJ_p5	petA-psbJ	5	CCCGTGTCCAAGGTCTTTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.3	58.1	0.0	0.0	1.406083	46833	47227	500-583	1.000000	77.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	TCCAGGTAGCCTATAGTGCC	ACGGGTGCCACAGATAGATA	20	20	55.0	50.0	58.0	57.9	8.9	0.0	0.093262	49665	50018	988-1139	1.000000	91.3
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	TCCAGGTAGCCTATAGTGCC	TCGTTTCGGACACAACTGTT	20	20	55.0	45.0	58.0	58.0	8.9	0.0	0.024059	49665	50018	1577-1731	0.857143	85.8
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CCAGGTAGCCTATAGTGCCT	TCGTTTCGGACACAACTGTT	20	20	55.0	45.0	58.0	58.0	8.9	0.0	0.029842	49665	50018	1576-1730	0.857143	85.7
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	TCCAGGTAGCCTATAGTGCC	CTTTGGCCATGAACCTCCTT	20	20	55.0	50.0	58.0	58.1	8.9	6.1	0.082254	49665	50018	1522-1676	0.857143	85.6
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CCAGGTAGCCTATAGTGCCT	CTTTGGCCATGAACCTCCTT	20	20	55.0	50.0	58.0	58.1	8.9	6.1	0.088037	49665	50018	1521-1675	0.857143	85.5
psbT-psbN_p1	psbT-psbN	1	GCCTCCTAAGGTTCCGACTA	GGGTACGCCTTATATACCGC	20	20	55.0	55.0	58.2	57.7	0.0	0.0	0.511426	55612	55648	140-192	0.714286	67.0
psbT-psbN_p2	psbT-psbN	2	GCCTCCTAAGGTTCCGACTA	GATCCATTCGAGGAACACGG	20	20	55.0	55.0	58.2	58.4	0.0	0.0	0.655245	55612	55648	92-144	0.714286	66.9
psbT-psbN_p3	psbT-psbN	3	TTCGAGAGCCTCCTAAGGTT	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.8	57.7	0.0	0.0	0.517568	55612	55648	147-199	0.714286	66.8
psbT-psbN_p4	psbT-psbN	4	TTTCGAGAGCCTCCTAAGGT	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.8	57.7	0.0	0.0	0.517568	55612	55648	148-200	0.714286	66.8
psbT-psbN_p5	psbT-psbN	5	TTCGAGAGCCTCCTAAGGTT	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.661386	55612	55648	99-151	0.714286	66.6
petD_p1	petD	1	ACCCCTTCTTACTGCCGTAT	CCCAGAGACATACGTTGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.134657	57733	58949	1526-1552	1.000000	87.4
petD_p2	petD	2	ACCCCTTCTTACTGCCGTAT	CCCCAGAGACATACGTTGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.134657	57733	58949	1527-1553	1.000000	87.4
petD_p3	petD	3	ACGGGATTTGCCATGACTTT	CCCAGAGACATACGTTGTGG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.086789	57733	58949	1951-1977	1.000000	86.2
petD_p4	petD	4	ACGGGATTTGCCATGACTTT	CCCCAGAGACATACGTTGTG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.086789	57733	58949	1952-1978	1.000000	86.2
petD_p5	petD	5	ACGGGATTTGCCATGACTTT	TCCCCAGAGACATACGTTGT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.143623	57733	58949	1953-1979	1.000000	85.7
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TCGTTCCAAGCTCGAAGTAC	20	20	50.0	50.0	58.0	57.7	7.6	0.0	0.352111	67336	69744	2472-2472	0.142857	42.9
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GTTCCAAGCTCGAAGTACCA	20	20	50.0	50.0	58.0	57.5	7.6	0.0	0.459810	67336	69744	2470-2470	0.142857	42.0
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	CTTCATGCTCGTTCCAAGCT	20	20	50.0	50.0	58.0	58.6	7.6	0.0	0.562293	67336	69744	2480-2480	0.142857	41.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	TCTTCATGCTCGTTCCAAGC	20	20	50.0	50.0	58.0	58.6	7.6	0.0	0.566432	67336	69744	2481-2481	0.142857	41.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	TTCATGCTCGTTCCAAGCTC	20	20	50.0	50.0	58.0	58.6	7.6	0.0	0.566432	67336	69744	2479-2479	0.142857	41.2
rps7-trnV-GAC_p1	rps7-trnV-GAC	1	AACTTGATGAGTCGATCCGC	TCGTAGACAAAGCGGATTCG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.167961	73444	75928	1760-2896	0.571429	68.9
rps7-trnV-GAC_p2	rps7-trnV-GAC	2	CATATTTCGACCCGGACGTT	TCGTAGACAAAGCGGATTCG	20	20	50.0	50.0	58.1	58.1	0.7	0.0	0.165289	73444	75928	1847-2983	0.571429	68.8
rps7-trnV-GAC_p3	rps7-trnV-GAC	3	GCAGCATCCACTAATTCGGA	TCCATGCGCTTCAAGAAACT	20	20	50.0	45.0	58.0	58.1	0.0	9.7	0.150221	73444	75928	2966-2966	0.142857	44.5
rps7-trnV-GAC_p4	rps7-trnV-GAC	4	CATATTTCGACCCGGACGTT	TCCATGCGCTTCAAGAAACT	20	20	50.0	45.0	58.1	58.1	0.7	9.7	0.173213	73444	75928	2931-2931	0.142857	44.3
rps7-trnV-GAC_p5	rps7-trnV-GAC	5	AACTTGATGAGTCGATCCGC	TCCATGCGCTTCAAGAAACT	20	20	50.0	45.0	58.1	58.1	0.0	9.7	0.175885	73444	75928	2844-2844	0.142857	44.3
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CCTTGTTCCTACATGACCCG	CAGAAATCCGTCGGCTGTTA	20	20	55.0	50.0	58.0	58.0	0.0	6.3	0.028758	83683	84280	1327-1327	0.142857	45.5
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAATTCGATCAGGCATTCGC	CAGAAATCCGTCGGCTGTTA	20	20	50.0	50.0	58.0	58.0	0.0	6.3	0.032870	83683	84280	1305-1305	0.142857	45.5
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CCTTGTTCCTACATGACCCG	ATGTGGTCAGAAATCCGTCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.034321	83683	84280	1334-1334	0.142857	45.4
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAATTCGATCAGGCATTCGC	ATGTGGTCAGAAATCCGTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038432	83683	84280	1312-1312	0.142857	45.4
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	TGAAATGGCTGAGGAGAGGA	CAGAAATCCGTCGGCTGTTA	20	20	50.0	50.0	58.0	58.0	0.0	6.3	0.047714	83683	84280	800-800	0.142857	45.3
ndhF-rpl32_p1	ndhF-rpl32	1	AGGAACTGGAAGGGGAATGA	TTACGAATACGCTTTTTGGA	20	20	50.0	35.0	57.9	53.3	0.0	0.0	4.783540	87571	88455	752-1178	0.857143	42.4
ndhF-rpl32_p2	ndhF-rpl32	2	GGAGAAGACCCACCCCTATT	TTACGAATACGCTTTTTGGA	20	20	55.0	35.0	58.1	53.3	0.0	0.0	4.829084	87571	88455	777-1203	0.857143	42.0
ndhF-rpl32_p3	ndhF-rpl32	3	AGGAACTGGAAGGGGAATGA	ACGCTTTTTGGATATAGAAGTACG	20	24	50.0	37.5	57.9	57.2	0.0	0.0	4.883434	87571	88455	744-1170	0.857143	41.6
ndhF-rpl32_p4	ndhF-rpl32	4	AGGAACTGGAAGGGGAATGA	CGCTTTTTGGATATAGAAGTACGT	20	24	50.0	37.5	57.9	57.2	0.0	0.0	4.883434	87571	88455	743-1169	0.857143	41.6
ndhF-rpl32_p5	ndhF-rpl32	5	AGGAACTGGAAGGGGAATGA	TTTACGAATACGCTTTTTGGA	20	21	50.0	33.3	57.9	54.2	0.0	0.0	4.895946	87571	88455	753-1179	0.857143	41.5
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CAGAAATCCGTCGGCTGTTA	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	6.3	0.0	0.028758	98802	99399	1327-1327	0.142857	45.5
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CAGAAATCCGTCGGCTGTTA	CAATTCGATCAGGCATTCGC	20	20	50.0	50.0	58.0	58.0	6.3	0.0	0.032870	98802	99399	1305-1305	0.142857	45.5
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	ATGTGGTCAGAAATCCGTCG	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.034321	98802	99399	1334-1334	0.142857	45.4
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	ATGTGGTCAGAAATCCGTCG	CAATTCGATCAGGCATTCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038432	98802	99399	1312-1312	0.142857	45.4
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CAGAAATCCGTCGGCTGTTA	TGAAATGGCTGAGGAGAGGA	20	20	50.0	50.0	58.0	58.0	6.3	0.0	0.047714	98802	99399	800-800	0.142857	45.3
trnV-GAC-rps7_p1	trnV-GAC-rps7	1	TCGTAGACAAAGCGGATTCG	AACTTGATGAGTCGATCCGC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.167961	107176	109638	1760-2896	0.571429	70.2
trnV-GAC-rps7_p2	trnV-GAC-rps7	2	TCGTAGACAAAGCGGATTCG	CATATTTCGACCCGGACGTT	20	20	50.0	50.0	58.1	58.1	0.0	0.7	0.165289	107176	109638	1847-2983	0.571429	70.0
trnV-GAC-rps7_p3	trnV-GAC-rps7	3	TCCATGCGCTTCAAGAAACT	GCAGCATCCACTAATTCGGA	20	20	45.0	50.0	58.1	58.0	9.7	0.0	0.150221	107176	109638	2966-2966	0.142857	44.5
trnV-GAC-rps7_p4	trnV-GAC-rps7	4	TCCATGCGCTTCAAGAAACT	CATATTTCGACCCGGACGTT	20	20	45.0	50.0	58.1	58.1	9.7	0.7	0.173213	107176	109638	2931-2931	0.142857	44.3
trnV-GAC-rps7_p5	trnV-GAC-rps7	5	TCCATGCGCTTCAAGAAACT	AACTTGATGAGTCGATCCGC	20	20	45.0	50.0	58.1	58.1	9.7	0.0	0.175885	107176	109638	2844-2844	0.142857	44.3
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TCGTTCCAAGCTCGAAGTAC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.7	58.0	0.0	7.6	0.352111	113338	115746	2472-2472	0.142857	42.9
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	GTTCCAAGCTCGAAGTACCA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.5	58.0	0.0	7.6	0.459810	113338	115746	2470-2470	0.142857	42.0
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CTTCATGCTCGTTCCAAGCT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.6	58.0	0.0	7.6	0.562293	113338	115746	2480-2480	0.142857	41.2
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TCTTCATGCTCGTTCCAAGC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.6	58.0	0.0	7.6	0.566432	113338	115746	2481-2481	0.142857	41.2
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	TTCATGCTCGTTCCAAGCTC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.6	58.0	0.0	7.6	0.566432	113338	115746	2479-2479	0.142857	41.2