primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	20	21	45.0	47.6	58.0	56.9	0.0	0.0	2.089811	4811	5163	400-429	1.000000	70.2
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	CGCATAACTCGTACAACGGA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.1	56.9	0.0	0.0	2.152666	4811	5163	419-448	1.000000	69.5
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	20	22	45.0	45.5	58.0	57.9	0.0	0.0	2.173139	4811	5163	406-435	1.000000	69.5
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	CGCATAACTCGTACAACGGA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.1	57.9	0.0	0.0	2.235994	4811	5163	425-454	1.000000	68.8
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	ACAAAACGGGAAAGAGAGGG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	57.7	56.9	0.0	0.0	2.323548	4811	5163	455-484	1.000000	68.0
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	ACGCTTTATATTTCAGGAAACA	21	22	47.6	31.8	57.3	54.1	0.0	0.0	7.586994	5236	5454	229-275	1.000000	50.7
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	AGAACGCTTTATATTTCAGGAAACA	21	25	47.6	32.0	57.3	57.3	0.0	0.0	7.374730	5236	5454	232-278	1.000000	50.6
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	GAACGCTTTATATTTCAGGAAACA	21	24	47.6	33.3	57.3	56.0	0.0	0.0	7.662880	5236	5454	231-277	1.000000	50.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	AGAACGCTTTATATTTCAGGAAACAA	21	26	47.6	30.8	57.3	57.9	0.0	0.0	7.799038	5236	5454	232-278	1.000000	50.6
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGATAGGACAGAGGTCTTCT	AGAACGCTTTATATTTCAGGAAACA	21	25	47.6	32.0	56.9	57.3	0.0	0.0	7.733544	5236	5454	267-313	1.000000	49.8
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	8833	9434	632-687	1.000000	82.7
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	CACTCGAGGGCCATCATTAG	20	20	45.0	55.0	58.1	57.8	0.0	19.0	0.264603	8833	9434	597-652	1.000000	82.5
atpH-atpI_p3	atpH-atpI	3	AACGGAAGCAGCAGAAATCA	TCACTCGAGGGCCATCATTA	20	20	45.0	50.0	58.1	58.2	0.0	19.0	0.315551	8833	9434	598-653	1.000000	82.1
atpH-atpI_p4	atpH-atpI	4	GAAGAAAGCCCTACAGCCAA	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.333113	8833	9434	664-719	1.000000	81.9
atpH-atpI_p5	atpH-atpI	5	GAAGAAAGCCCTACAGCCAA	CACTCGAGGGCCATCATTAG	20	20	50.0	55.0	57.8	57.8	0.0	19.0	0.361195	8833	9434	629-684	1.000000	81.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.314369	22007	22218	266-276	1.000000	81.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TAAAGCAGCCCAAGCGATAC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.418433	22007	22218	264-274	1.000000	80.2
trnC-GCA-petN_p3	trnC-GCA-petN	3	GTATCATTTTGGCGGCATGG	ATTAAAGCAGCCCAAGCGAT	20	20	50.0	45.0	57.9	58.2	14.3	0.0	0.370837	22007	22218	318-328	1.000000	80.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCGATA	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.493756	22007	22218	265-275	1.000000	79.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTATCATTTTGGCGGCATGG	TAAAGCAGCCCAAGCGATAC	20	20	50.0	50.0	57.9	58.3	14.3	0.0	0.474902	22007	22218	316-326	1.000000	79.2
rps14-psaB_p1	rps14-psaB	1	ACTCAATGAGGGAACTTTGCT	TTGATTGCCTCCACATCAGG	21	20	42.9	50.0	57.5	57.9	15.4	0.0	1.648883	29832	29976	290-292	1.000000	67.9
rps14-psaB_p2	rps14-psaB	2	CACTCAATGAGGGAACTTTGC	TTGATTGCCTCCACATCAGG	21	20	47.6	50.0	57.4	57.9	15.4	0.0	1.699186	29832	29976	291-293	1.000000	67.5
rps14-psaB_p3	rps14-psaB	3	ACTCAATGAGGGAACTTTGCT	GCTCTTTCCATTGTGCAAGC	21	20	42.9	50.0	57.5	58.3	15.4	0.0	1.809159	29832	29976	368-370	1.000000	66.4
rps14-psaB_p4	rps14-psaB	4	ACTCAATGAGGGAACTTTGCT	TGGCTCTTTCCATTGTGCAA	21	20	42.9	45.0	57.5	58.3	15.4	0.0	1.813420	29832	29976	370-372	1.000000	66.4
rps14-psaB_p5	rps14-psaB	5	CACTCAATGAGGGAACTTTGC	GCTCTTTCCATTGTGCAAGC	21	20	47.6	50.0	57.4	58.3	15.4	0.0	1.859462	29832	29976	369-371	1.000000	66.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TGAGGCGCATAATTGGTTGA	20	20	50.0	45.0	57.9	57.9	4.1	10.8	0.236634	34460	35087	747-751	1.000000	79.9
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	34460	35087	731-735	1.000000	79.6
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	34460	35087	735-739	1.000000	79.4
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.405330	34460	35087	730-734	1.000000	78.6
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	20	20	50.0	45.0	57.9	57.6	4.1	15.7	0.466242	34460	35087	724-728	1.000000	78.1
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	GAATTGGGTGGGTGAAGTGT	21	20	47.6	50.0	58.0	58.0	8.2	0.0	1.045108	37093	37650	667-686	1.000000	75.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	GTGAATTGGGTGGGTGAAGT	21	20	47.6	50.0	58.0	58.0	8.2	0.0	1.045108	37093	37650	669-688	1.000000	75.3
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	CAGTGAATTGGGTGGGTGAA	21	20	47.6	50.0	58.0	58.0	8.2	0.0	1.046528	37093	37650	671-690	1.000000	75.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	TGAATTGGGTGGGTGAAGTG	21	20	47.6	50.0	58.0	58.0	8.2	0.0	1.046528	37093	37650	668-687	1.000000	75.3
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	37093	37650	628-647	1.000000	74.8
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AGTTAATCCCGGTAAAGCGC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.472573	38563	38973	525-540	1.000000	79.1
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	AGTTAATCCCGGTAAAGCGC	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.580327	38563	38973	493-508	1.000000	78.4
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	AGTTAATCCCGGTAAAGCGC	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.3	57.5	0.0	7.6	0.840342	38563	38973	533-548	1.000000	76.1
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	AGTTAATCCCGGTAAAGCGC	CAGAGGTTAGAGCATCGCAT	20	20	50.0	50.0	58.3	57.5	0.0	0.0	0.862669	38563	38973	523-538	1.000000	76.0
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	AGTTAATCCCGGTAAAGCGC	TTAGGCGCGTATTATGTGGG	20	20	50.0	50.0	58.3	57.8	0.0	11.1	0.497668	38563	38973	573-573	0.500000	56.0
trnF-GAA-trnM-CAU_p1	trnF-GAA-trnM-CAU	1	CCTCGTGTCACCAGTTCAAA	CGCCGTATGAAAGCAGTACT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129325	40420	40963	620-624	1.000000	82.7
trnF-GAA-trnM-CAU_p2	trnF-GAA-trnM-CAU	2	GGGGATATGGCGAAATTGGT	CGCCGTATGAAAGCAGTACT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.082053	40420	40963	1278-1284	1.000000	82.0
trnF-GAA-trnM-CAU_p3	trnF-GAA-trnM-CAU	3	GGGGATATGGCGAAATTGGT	TTGAGAATGCGCCTTAACGA	20	20	50.0	45.0	58.0	57.9	0.0	4.2	0.092125	40420	40963	1796-1808	1.000000	81.6
trnF-GAA-trnM-CAU_p4	trnF-GAA-trnM-CAU	4	GGGGATATGGCGAAATTGGT	AAGAAGCCCGTCAAACTCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120389	40420	40963	1672-1684	1.000000	81.5
trnF-GAA-trnM-CAU_p5	trnF-GAA-trnM-CAU	5	GGGGATATGGCGAAATTGGT	CAAGAAGCCCGTCAAACTCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120389	40420	40963	1673-1685	1.000000	81.5
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	TACCAAACTGCGCTACATCC	GTACTTAGTACTGGTGCCGC	20	20	50.0	55.0	58.0	58.1	9.7	14.6	0.099947	54012	54350	408-410	1.000000	81.4
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	TACCAAACTGCGCTACATCC	ACGCATCTGGGAAAAATCGA	20	20	50.0	45.0	58.0	57.9	9.7	0.8	0.126492	54012	54350	470-472	1.000000	80.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	AAACAAACGCGCTACCAAAC	GTACTTAGTACTGGTGCCGC	20	20	45.0	55.0	58.2	58.1	5.7	14.6	0.251319	54012	54350	420-422	1.000000	80.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAAAACAAACGCGCTACCAA	GTACTTAGTACTGGTGCCGC	20	20	45.0	55.0	58.2	58.1	5.7	14.6	0.252574	54012	54350	422-424	1.000000	80.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TTGTACCCAAAACAAACGCG	GTACTTAGTACTGGTGCCGC	20	20	45.0	55.0	57.8	58.1	3.5	14.6	0.271730	54012	54350	429-431	1.000000	79.9
rpl20-rps12_p1	rpl20-rps12	1	TTTTGTTCTACGCCTCCGAG	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.195663	55913	56730	873-893	1.000000	81.1
rpl20-rps12_p2	rpl20-rps12	2	TCCTTGAATGAGCTCCTCGA	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	58.1	57.9	12.1	0.0	0.268484	55913	56730	916-936	1.000000	80.4
rpl20-rps12_p3	rpl20-rps12	3	GAGCTCCTCGAAAACTTGCT	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	58.2	57.9	0.9	0.0	0.319047	55913	56730	907-927	1.000000	80.0
rpl20-rps12_p4	rpl20-rps12	4	TCCTCGAAAACTTGCTGCAA	CGCCGAGGAACATGTACTAG	20	20	45.0	55.0	58.3	57.9	15.2	0.0	0.446757	55913	56730	903-923	1.000000	79.0
rpl20-rps12_p5	rpl20-rps12	5	TTTTGTTCTACGCCTCCGAG	GCCGAGGAACATGTACTAGG	20	20	50.0	55.0	57.9	57.5	0.0	19.0	0.585315	55913	56730	872-892	1.000000	77.9
petD-rpoA_p1	petD-rpoA	1	TACAACAGCCCTTTGGTTGG	GAGCATTTTCGAATAGCAGATGT	20	23	50.0	39.1	58.3	57.6	8.4	16.1	3.716909	64758	65020	416-423	1.000000	53.1
petD-rpoA_p2	petD-rpoA	2	CGTCCAATAGCGACAACCAT	GAGCATTTTCGAATAGCAGATGT	20	23	50.0	39.1	58.3	57.6	0.0	16.1	3.763427	64758	65020	451-458	1.000000	52.5
petD-rpoA_p3	petD-rpoA	3	TACAACAGCCCTTTGGTTGG	AGCATTTTCGAATAGCAGATGT	20	22	50.0	36.4	58.3	56.5	8.4	16.1	3.807478	64758	65020	415-422	1.000000	52.4
petD-rpoA_p4	petD-rpoA	4	CGTCCAATAGCGACAACCAT	AGCATTTTCGAATAGCAGATGT	20	22	50.0	36.4	58.3	56.5	0.0	16.1	3.853996	64758	65020	450-457	1.000000	51.8
petD-rpoA_p5	petD-rpoA	5	TACAACAGCCCTTTGGTTGG	AGAGCATTTTCGAATAGCAGA	20	21	50.0	38.1	58.3	55.4	8.4	16.1	3.896168	64758	65020	417-424	1.000000	51.7
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	83605	84880	1372-1593	1.000000	79.2
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	83605	84880	1376-1597	1.000000	79.2
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	83605	84880	1371-1592	1.000000	79.2
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	83605	84880	1374-1595	1.000000	78.2
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	ACGTAAGGCAGAGTTTGGTT	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.4	57.8	0.0	0.0	0.796805	83605	84880	1362-1583	1.000000	76.2
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TTGGTAAACACGCTGCTCTT	TGTTCAAAAGTCGAAAACAGCA	20	22	45.0	36.4	58.0	57.6	0.0	0.0	2.465302	93045	93245	289-377	1.000000	63.6
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	AACACGCTGCTCTTAGGAAG	TGTTCAAAAGTCGAAAACAGCA	20	22	50.0	36.4	57.9	57.6	0.0	0.0	2.513150	93045	93245	283-371	1.000000	63.3
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AAAGGCCGCTATGGTGAAAT	TGTTCAAAAGTCGAAAACAGCA	20	22	45.0	36.4	57.9	57.6	0.0	0.0	2.570971	93045	93245	308-396	1.000000	62.6
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAGGCCGCTATGGTGAAATT	TGTTCAAAAGTCGAAAACAGCA	20	22	45.0	36.4	57.9	57.6	0.0	0.0	2.570971	93045	93245	307-395	1.000000	62.6
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	ATTGGTAAACACGCTGCTCT	TGTTCAAAAGTCGAAAACAGCA	20	22	45.0	36.4	57.8	57.6	0.0	0.0	2.612100	93045	93245	290-378	1.000000	62.5
ycf1_p1	ycf1	1	TTCTGTCGCGCCTTACTTAG	ACCAAAACACTTTTCCGGGA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.420290	96478	96627	1086-1089	1.000000	78.5
ycf1_p2	ycf1	2	TCTGTCGCGCCTTACTTAGT	ACCAAAACACTTTTCCGGGA	20	20	50.0	45.0	58.8	57.9	0.0	0.0	0.975612	96478	96627	1085-1088	1.000000	74.0
ycf1_p3	ycf1	3	CTGTCGCGCCTTACTTAGTA	ACCAAAACACTTTTCCGGGA	20	20	50.0	45.0	57.2	57.9	0.0	0.0	0.985307	96478	96627	1084-1087	1.000000	74.0
ycf1_p4	ycf1	4	ATTCTGTCGCGCCTTACTTA	ACCAAAACACTTTTCCGGGA	20	20	45.0	45.0	57.0	57.9	0.0	0.0	1.100386	96478	96627	1087-1090	1.000000	73.0
ycf1_p5	ycf1	5	TATTCTGTCGCGCCTTACTT	ACCAAAACACTTTTCCGGGA	20	20	45.0	45.0	57.0	57.9	0.0	0.0	1.100386	96478	96627	1088-1091	1.000000	73.0
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	107266	108541	1372-1593	1.000000	79.2
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	107266	108541	1376-1597	1.000000	79.2
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	107266	108541	1371-1592	1.000000	79.2
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	107266	108541	1374-1595	1.000000	78.2
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	GATGACTTCCACCACGTCAA	ACGTAAGGCAGAGTTTGGTT	20	20	50.0	45.0	57.8	57.4	0.0	0.0	0.796805	107266	108541	1362-1583	1.000000	76.2