primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	trnK-rps16	1	CCTTTCAGGATCAGTCGTGG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.0	58.1	0.0	16.9	0.091837	4332	5042	1472-2349	1.000000	91.3
trnK-rps16_p2	trnK-rps16	2	TCAGGATCAGTCGTGGTCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.151370	4332	5042	1468-2345	0.937500	88.3
trnK-rps16_p3	trnK-rps16	3	TTCAGGATCAGTCGTGGTCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.151370	4332	5042	1469-2346	0.937500	88.3
trnK-rps16_p4	trnK-rps16	4	CCTTTCAGGATCAGTCGTGG	TCGATCCCGAAGAGAAGGAA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.155059	4332	5042	947-1053	0.750000	80.8
trnK-rps16_p5	trnK-rps16	5	CCTTTCAGGATCAGTCGTGG	GATTTCCTTAAAACGGGCGC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.111592	4332	5042	945-962	0.250000	52.4
trnS-trnG_p1	trnS-trnG	1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.699660	6944	7631	732-816	1.000000	62.4
trnS-trnG_p2	trnS-trnG	2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	2.0	0.0	3.738818	6944	7631	730-814	1.000000	62.1
trnS-trnG_p3	trnS-trnG	3	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	57.8	56.2	0.0	0.0	3.940131	6944	7631	733-817	1.000000	60.5
trnS-trnG_p4	trnS-trnG	4	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.6	55.5	0.9	0.0	3.945791	6944	7631	727-811	1.000000	60.4
trnS-trnG_p5	trnS-trnG	5	AACGCTTTAGTCCACTCAGC	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.2	56.2	2.0	0.0	3.979289	6944	7631	731-815	1.000000	60.2
trnG-trnR_p1	trnG-trnR	1	TGATCACTCGACGTCGACTA	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	35.0	3.0	4.076382	8424	8565	278-316	0.875000	54.4
trnG-trnR_p2	trnG-trnR	2	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.105791	8424	8565	250-288	0.875000	54.2
trnG-trnR_p3	trnG-trnR	3	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.109107	8424	8565	256-294	0.875000	54.1
trnG-trnR_p4	trnG-trnR	4	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	57.9	53.9	33.4	3.0	4.132047	8424	8565	270-308	0.875000	53.9
trnG-trnR_p5	trnG-trnR	5	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.1	53.9	0.0	3.0	4.151221	8424	8565	258-296	0.875000	53.8
trnR-atpA_p1	trnR-atpA	1	TGATCACTCGACGTCGACTA	GGGTCGTGAAGCTTATCCAG	20	20	50.0	55.0	58.0	58.1	35.0	0.0	0.059104	8638	8942	1250-1313	0.937500	89.0
trnR-atpA_p2	trnR-atpA	2	TGATCACTCGACGTCGACTA	GGTCGTGAAGCTTATCCAGG	20	20	50.0	55.0	58.0	58.1	35.0	0.0	0.059104	8638	8942	1249-1312	0.937500	89.0
trnR-atpA_p3	trnR-atpA	3	AGCCTTCCAAGCTAACGATG	GGGTCGTGAAGCTTATCCAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.088513	8638	8942	1222-1285	0.937500	88.8
trnR-atpA_p4	trnR-atpA	4	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.088513	8638	8942	1221-1284	0.937500	88.8
trnR-atpA_p5	trnR-atpA	5	ACCCTTAGCCTTCCAAGCTA	GGTCGTGAAGCTTATCCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.091830	8638	8942	1227-1290	0.937500	88.8
atpI-rps2_p1	atpI-rps2	1	TGGCCTACTTCTACACCAGA	TTCCAGCAAATGATGACGCT	20	20	50.0	45.0	57.4	58.2	3.3	0.0	0.790896	13793	14037	420-446	1.000000	83.2
atpI-rps2_p2	atpI-rps2	2	TGGCCTACTTCTACACCAGA	GCAATTTGTGAGGGTCGTTC	20	20	50.0	50.0	57.4	57.7	3.3	0.0	0.947795	13793	14037	361-387	1.000000	82.2
atpI-rps2_p3	atpI-rps2	3	TGGCCTACTTCTACACCAGA	TTGTGAGGGTCGTTCTAGCT	20	20	50.0	50.0	57.4	58.4	3.3	0.0	0.986943	13793	14037	356-382	1.000000	81.9
atpI-rps2_p4	atpI-rps2	4	TGGCCTACTTCTACACCAGA	TTTGTGAGGGTCGTTCTAGC	20	20	50.0	50.0	57.4	57.5	3.3	0.0	1.066595	13793	14037	357-383	1.000000	81.2
atpI-rps2_p5	atpI-rps2	5	TGGCCTACTTCTACACCAGA	TGTGAGGGTCGTTCTAGCTA	20	20	50.0	50.0	57.4	57.5	3.3	0.0	1.118128	13793	14037	355-381	1.000000	80.8
trnD-trnY_p1	trnD-trnY	1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	28954	29367	480-508	1.000000	86.3
trnD-trnY_p2	trnD-trnY	2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	28954	29367	479-507	1.000000	86.3
trnD-trnY_p3	trnD-trnY	3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	28954	29367	501-529	1.000000	85.3
trnD-trnY_p4	trnD-trnY	4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	28954	29367	500-528	1.000000	85.1
trnD-trnY_p5	trnD-trnY	5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.889785	28954	29367	534-562	1.000000	84.5
trnE-trnT_p1	trnE-trnT	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	29584	30416	913-939	1.000000	89.8
trnE-trnT_p2	trnE-trnT	2	ACTTCCTTTCGTGGTACCCT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.3	58.0	10.1	0.0	0.280168	29584	30416	957-983	1.000000	89.8
trnE-trnT_p3	trnE-trnT	3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	29584	30416	892-918	1.000000	89.7
trnE-trnT_p4	trnE-trnT	4	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	29584	30416	892-918	1.000000	89.4
trnE-trnT_p5	trnE-trnT	5	ACTTCCTTTCGTGGTACCCT	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	58.3	58.1	10.1	0.0	0.338265	29584	30416	936-962	1.000000	89.3
rps4-trnT_p1	rps4-trnT	1	TAAGATCGCTTCCTGCCCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.214880	44746	45215	499-630	1.000000	90.3
rps4-trnT_p2	rps4-trnT	2	TAAGATCGCTTCCTGCCCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.322633	44746	45215	467-598	1.000000	89.4
rps4-trnT_p3	rps4-trnT	3	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137323	44746	45215	517-648	0.937500	88.4
rps4-trnT_p4	rps4-trnT	4	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.245076	44746	45215	485-616	0.937500	87.5
rps4-trnT_p5	rps4-trnT	5	CGGAGCGTGATTGGTTTCTA	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.0	57.5	0.0	7.6	0.505091	44746	45215	525-656	0.937500	85.5
trnT-trnL_p1	trnT-trnL	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.283643	45289	46117	858-963	1.000000	89.7
trnT-trnL_p2	trnT-trnL	2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.314721	45289	46117	909-1014	1.000000	89.5
trnT-trnL_p3	trnT-trnL	3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	45289	46117	861-966	1.000000	89.0
trnT-trnL_p4	trnT-trnL	4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.391397	45289	46117	890-995	1.000000	88.9
trnT-trnL_p5	trnT-trnL	5	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	33.1	10.2	0.403626	45289	46117	912-1017	1.000000	88.8
trnF-ndhJ_p1	trnF-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.062041	46943	47625	663-857	1.000000	91.5
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.132182	46943	47625	660-854	1.000000	90.9
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.276511	46943	47625	670-864	1.000000	89.8
trnF-ndhJ_p4	trnF-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATCCACGACTGAAACGGATC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.347149	46943	47625	662-856	1.000000	89.2
trnF-ndhJ_p5	trnF-ndhJ	5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGCTGG	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.383476	46943	47625	794-794	0.062500	39.4
ndhC-trnV_p1	ndhC-trnV	1	TGCCAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	21	20	42.9	55.0	58.1	58.7	0.0	0.0	1.808053	49315	50180	795-1041	1.000000	77.5
ndhC-trnV_p2	ndhC-trnV	2	TGCCAAAACAGGAATAGCACT	TTCGAGTCCGTATAGCCCTA	21	20	42.9	50.0	58.1	57.1	0.0	0.0	2.061623	49315	50180	794-1040	1.000000	75.5
ndhC-trnV_p3	ndhC-trnV	3	TGCCAAAACAGGAATAGCAC	GTTCGAGTCCGTATAGCCCT	20	20	45.0	55.0	56.6	58.7	0.0	0.0	2.077732	49315	50180	795-1041	1.000000	75.4
ndhC-trnV_p4	ndhC-trnV	4	TGCCAAAACAGGAATAGCACT	TCGAACTCTTTTACCGAGCAG	21	21	42.9	47.6	58.1	58.1	0.0	0.0	2.181994	49315	50180	823-1069	1.000000	74.5
ndhC-trnV_p5	ndhC-trnV	5	TGCCAAAACAGGAATAGCACT	TCGAACTCTTTTACCGAGCA	21	20	42.9	45.0	58.1	56.9	0.0	0.0	2.217059	49315	50180	823-1069	1.000000	74.3
ndhF-rpl32_p1	ndhF-rpl32	1	GCAATAGGTCGTGTGAACCA	TGCATCAAGTTTGTCCTAGGG	20	21	50.0	47.6	58.2	57.9	0.0	9.5	1.268540	111240	112081	1657-1724	0.625000	66.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGCAATAGGTCGTGTGAACC	TGCATCAAGTTTGTCCTAGGG	20	21	50.0	47.6	58.2	57.9	0.0	9.5	1.268540	111240	112081	1658-1725	0.625000	66.7
ndhF-rpl32_p3	ndhF-rpl32	3	GGTCGTGTGAACCAAAAACC	TGCATCAAGTTTGTCCTAGGG	20	21	50.0	47.6	57.8	57.9	0.0	9.5	1.269666	111240	112081	1651-1718	0.625000	66.7
ndhF-rpl32_p4	ndhF-rpl32	4	GTCCACATACGTCGAAGGTT	TGCATCAAGTTTGTCCTAGGG	20	21	50.0	47.6	57.6	57.9	0.0	9.5	1.430813	111240	112081	1267-1334	0.625000	65.6
ndhF-rpl32_p5	ndhF-rpl32	5	AGTCCACATACGTCGAAGGT	TGCATCAAGTTTGTCCTAGGG	20	21	50.0	47.6	58.5	57.9	0.0	9.5	1.529381	111240	112081	1268-1335	0.625000	64.8
rpl32-trnL_p1	rpl32-trnL	1	GGAAGGGATATTGGACATCGT	TAACCACTGGGACTGGAGAG	21	20	47.6	55.0	57.2	58.1	0.0	0.0	1.832992	112241	113005	1324-1650	0.937500	74.8
rpl32-trnL_p2	rpl32-trnL	2	GGAAGGGATATTGGACATCGT	AGATCTTTTGCCGCTACTCG	21	20	47.6	50.0	57.2	58.1	0.0	0.0	1.843682	112241	113005	801-1127	0.937500	74.8
rpl32-trnL_p3	rpl32-trnL	3	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	57.2	57.9	0.0	0.0	1.895854	112241	113005	752-1078	0.937500	74.3
rpl32-trnL_p4	rpl32-trnL	4	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	21	20	47.6	45.0	57.2	57.8	0.0	0.0	1.956581	112241	113005	745-1071	0.937500	73.8
rpl32-trnL_p5	rpl32-trnL	5	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	21	20	47.6	55.0	57.2	57.8	0.0	0.0	1.967872	112241	113005	750-1076	0.937500	73.8
ycf1_p1	ycf1	1	TTTGCCAGAACCAAACGGTA	GGATCTCTCGCGGAAGAATC	20	20	45.0	55.0	57.9	57.9	0.0	6.1	0.113146	125602	125721	1184-1322	0.687500	78.6
ycf1_p2	ycf1	2	GTTTCGGATAGTGGAACCCC	GGATCTCTCGCGGAAGAATC	20	20	55.0	55.0	58.0	57.9	0.0	6.1	0.093018	125602	125721	982-1120	0.625000	76.3
ycf1_p3	ycf1	3	CCAGAACCAAACGGTAGGTC	GGATCTCTCGCGGAAGAATC	20	20	55.0	55.0	58.2	57.9	5.8	6.1	0.254313	125602	125721	1225-1318	0.437500	67.5
ycf1_p4	ycf1	4	TGCCAAGGTTTCAGACGAAA	GGATCTCTCGCGGAAGAATC	20	20	45.0	55.0	58.0	57.9	0.0	6.1	0.104475	125602	125721	1096-1111	0.125000	48.5
ycf1_p5	ycf1	5	ATGCCAAGGTTTCAGACGAA	GGATCTCTCGCGGAAGAATC	20	20	45.0	55.0	57.7	57.9	0.0	6.1	0.329266	125602	125721	1097-1112	0.125000	46.7