primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	78	737	730-799	1.000000	79.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	78	737	744-813	1.000000	79.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	78	737	743-812	1.000000	79.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	78	737	745-814	1.000000	79.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	737	779-848	1.000000	77.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.080725	6760	7452	784-805	1.000000	82.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGCTTTCTACCACAGCGTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	0.0	16.9	0.114308	6760	7452	734-755	1.000000	82.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TTGCTTTCTACCACAGCGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	0.0	16.9	0.114308	6760	7452	735-756	1.000000	82.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6760	7452	745-766	1.000000	81.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTTTGACATCCACAGCCGAT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.274928	6760	7452	775-796	1.000000	81.2
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	GTTTTCGACTTCAGGAACAA	21	20	47.6	40.0	57.3	54.1	0.0	0.0	5.597386	10977	11255	308-324	1.000000	47.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	GTTTTCGACTTCAGGAACAAT	21	21	47.6	38.1	57.3	54.7	0.0	0.0	5.975046	10977	11255	308-324	1.000000	47.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GTTCAAATCCTATTGGACGCA	GTTTTCGACTTCAGGAACAA	21	20	42.9	40.0	56.6	54.1	0.0	0.0	6.300329	10977	11255	307-323	1.000000	47.0
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TGGATAGGACAGAGGTCTTCT	GTTTTCGACTTCAGGAACAA	21	20	47.6	40.0	56.9	54.1	0.0	0.0	5.956199	10977	11255	343-359	1.000000	46.4
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	ATGAAAAGCGTCCATTGTCT	GTTTTCGACTTCAGGAACAA	20	20	40.0	40.0	55.6	54.1	0.0	0.0	6.259203	10977	11255	365-381	1.000000	46.0
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	32630	33116	557-557	1.000000	76.1
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	32630	33116	556-556	1.000000	76.1
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	32630	33116	578-578	1.000000	75.2
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	32630	33116	577-577	1.000000	75.0
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.889785	32630	33116	611-611	1.000000	74.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33333	34406	1150-1161	1.000000	80.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33333	34406	1148-1159	1.000000	80.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	33333	34406	1204-1215	1.000000	80.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	33333	34406	1167-1178	1.000000	80.6
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33333	34406	1127-1138	1.000000	80.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	51171	52610	1413-1490	1.000000	84.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	51171	52610	1421-1498	1.000000	83.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	51171	52610	1445-1522	1.000000	83.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CCAAGGCTCAATCCAATCCA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.281186	51171	52610	1451-1528	1.000000	83.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ACCAAGGCTCAATCCAATCC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.282713	51171	52610	1452-1529	1.000000	83.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGACCCTTTCTAATCGGAGC	TGGGTCTTTGAAATGGGTCG	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.337760	55958	57318	1560-1565	1.000000	79.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGACCCTTTCTAATCGGAGC	GGGTCTTTGAAATGGGTCGA	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.436248	55958	57318	1559-1564	1.000000	78.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGACCCTTTCTAATCGGAGC	AATGCGTGTTGGGTCTTTGA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.555692	55958	57318	1569-1574	1.000000	77.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGACCCTTTCTAATCGGAGC	ATGCGTGTTGGGTCTTTGAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.555692	55958	57318	1568-1573	1.000000	77.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGACCCTTTCTAATCGGAGC	TCTGTTTTACCGAGAAGGCC	20	20	55.0	50.0	57.8	57.5	0.0	0.0	0.709613	55958	57318	1514-1519	1.000000	76.7
rbcL-accD_p1	rbcL-accD	1	TTGAATGCTACTGCGGGTAC	AGCCGGATCTTCGTTTACAC	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.024512	62862	63683	1674-1723	1.000000	81.4
rbcL-accD_p2	rbcL-accD	2	TTGAATGCTACTGCGGGTAC	GCCGGATCTTCGTTTACACT	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.024512	62862	63683	1673-1722	1.000000	81.4
rbcL-accD_p3	rbcL-accD	3	TTGAATGCTACTGCGGGTAC	TAGAGCTGTCACTCCAACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028223	62862	63683	1720-1769	1.000000	81.3
rbcL-accD_p4	rbcL-accD	4	TTGAATGCTACTGCGGGTAC	ACCATTTCCACCTTCACCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023166	62862	63683	2106-2106	0.500000	59.8
rbcL-accD_p5	rbcL-accD	5	TTGAATGCTACTGCGGGTAC	CCATTTCCACCTTCACCACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024586	62862	63683	2105-2105	0.500000	59.8
accD_p1	accD	1	CCAGGATTCTCCCAAACGAG	GCGGGCTTTGACTTTGATTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.047226	63684	65636	2294-2458	1.000000	82.6
accD_p2	accD	2	CTCCAGGATTCTCCCAAACG	GCGGGCTTTGACTTTGATTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.047226	63684	65636	2296-2460	1.000000	82.5
accD_p3	accD	3	CCAGGATTCTCCCAAACGAG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.056842	63684	65636	2880-2880	0.500000	59.5
accD_p4	accD	4	CTCCAGGATTCTCCCAAACG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.056842	63684	65636	2882-2882	0.500000	59.5
accD_p5	accD	5	CCAGGATTCTCCCAAACGAG	GAGGGAAAGCTGCTGAAAGT	20	20	55.0	50.0	58.0	58.1	0.0	1.4	0.126900	63684	65636	2833-2833	0.500000	59.0
petA-psbJ_p1	petA-psbJ	1	CTACGTGTCCAAGGCCTTTT	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.1	57.6	17.6	0.0	0.527849	70233	71377	1285-1294	1.000000	76.6
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.574912	70233	71377	1273-1282	1.000000	76.3
petA-psbJ_p3	petA-psbJ	3	GATCCACTACGTGTCCAAGG	ATTCTGGATTGGGTTCGTCC	20	20	55.0	50.0	57.7	57.6	0.0	0.0	0.715311	70233	71377	1291-1300	1.000000	75.1
petA-psbJ_p4	petA-psbJ	4	TACGTGTCCAAGGCCTTTTG	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.4	57.6	17.6	0.0	0.820035	70233	71377	1284-1293	1.000000	74.3
petA-psbJ_p5	petA-psbJ	5	TGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	20	20	45.0	50.0	57.5	57.6	17.6	0.0	0.872659	70233	71377	1280-1289	1.000000	73.9
clpP1_p1	clpP1	1	TACGACGTAACCGACTACGA	TATGCACACGATACCAAGGC	20	20	50.0	50.0	58.0	58.1	19.0	0.0	0.066561	77803	78540	1641-1947	1.000000	84.5
clpP1_p2	clpP1	2	CGTTTACGACGTAACCGACT	TATGCACACGATACCAAGGC	20	20	50.0	50.0	58.0	58.1	27.1	0.0	0.101424	77803	78540	1645-1951	1.000000	84.2
clpP1_p3	clpP1	3	TACGACGTAACCGACTACGA	GGAGGGATCAAAAACAGCCA	20	20	50.0	50.0	58.0	58.1	19.0	0.0	0.091310	77803	78540	1757-2063	1.000000	84.0
clpP1_p4	clpP1	4	TACGACGTAACCGACTACGA	GAACATGCCTTGTCTCCACA	20	20	50.0	50.0	58.0	58.1	19.0	0.0	0.118446	77803	78540	1796-2102	1.000000	83.7
clpP1_p5	clpP1	5	GGAAAGAAGACGAAGCGACT	TATGCACACGATACCAAGGC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.119310	77803	78540	1321-1321	0.500000	59.0
clpP1-psbB_p1	clpP1-psbB	1	TCACGAAAATGACTAGCGGG	CAGATAGCTGCCAAGAAGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038687	78541	79057	1256-1347	1.000000	85.2
clpP1-psbB_p2	clpP1-psbB	2	TCACGAAAATGACTAGCGGG	AGGTTGTACCCTTCCGGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033144	78541	79057	1458-1549	1.000000	84.6
clpP1-psbB_p3	clpP1-psbB	3	TCACGAAAATGACTAGCGGG	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024773	78541	79057	1423-1423	0.500000	59.8
clpP1-psbB_p4	clpP1-psbB	4	GTTGTCGGTAAAGCACGTTG	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033476	78541	79057	1113-1113	0.500000	59.7
clpP1-psbB_p5	clpP1-psbB	5	GCCATTGAAGCAACCAATCC	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034526	78541	79057	1330-1330	0.500000	59.7
rps8-rpl14_p1	rps8-rpl14	1	ATGCGAACCGTTCCTTTTCT	GCTCGGGAATTGAGACAGTT	20	20	45.0	50.0	58.1	57.9	5.7	0.0	0.208395	87342	87516	315-322	1.000000	80.6
rps8-rpl14_p2	rps8-rpl14	2	ATGCGAACCGTTCCTTTTCT	AATTTTTGGTGCGATCGCTC	20	20	45.0	45.0	58.1	57.7	5.7	37.0	0.370363	87342	87516	331-338	1.000000	79.2
rps8-rpl14_p3	rps8-rpl14	3	ATGCGAACCGTTCCTTTTCT	GAATTTTTGGTGCGATCGCT	20	20	45.0	45.0	58.1	57.7	5.7	37.0	0.370363	87342	87516	332-339	1.000000	79.2
rps8-rpl14_p4	rps8-rpl14	4	ATGCGAACCGTTCCTTTTCT	GATCGCTCGGGAATTGAGAC	20	20	45.0	55.0	58.1	58.5	5.7	0.0	0.615070	87342	87516	319-326	1.000000	77.3
rps8-rpl14_p5	rps8-rpl14	5	ATGCGAACCGTTCCTTTTCT	TCGAATTTTTGGTGCGATCG	20	20	45.0	45.0	58.1	57.5	5.7	11.7	0.626793	87342	87516	334-341	1.000000	77.1
rpl22-rps19_p1	rpl22-rps19	1	AGTTGGATATGGGTTCCTCCT	ACGGATCGTATGGTAGGACA	21	20	47.6	50.0	57.8	57.6	0.0	0.0	1.504899	90875	90950	211-211	1.000000	68.7
rpl22-rps19_p2	rpl22-rps19	2	AGTTGGATATGGGTTCCTCCT	TTGGGAGAATTCGCACCTAC	21	20	47.6	50.0	57.8	57.6	0.0	9.1	1.548132	90875	90950	187-187	1.000000	68.5
rpl22-rps19_p3	rpl22-rps19	3	AGTTGGATATGGGTTCCTCCT	TGGGAGAATTCGCACCTACT	21	20	47.6	50.0	57.8	58.4	0.0	9.1	1.583038	90875	90950	186-186	1.000000	68.2
rpl22-rps19_p4	rpl22-rps19	4	AGTTGGATATGGGTTCCTCCT	AATTGGGAGAATTCGCACCT	21	20	47.6	45.0	57.8	57.5	0.0	9.1	1.666000	90875	90950	189-189	1.000000	67.5
rpl22-rps19_p5	rpl22-rps19	5	GTTGGATATGGGTTCCTCCT	ACGGATCGTATGGTAGGACA	20	20	50.0	50.0	56.3	57.6	0.0	0.0	2.080359	90875	90950	210-210	1.000000	64.1
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	CCTAGTTGATCCTGATTCGACA	GAATGGTTAAAGCGCCCAAC	22	20	45.5	50.0	57.3	58.0	0.0	2.3	2.664963	93067	93691	567-783	1.000000	66.1
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	TGTCCTAGTTGATCCTGATTCG	GAATGGTTAAAGCGCCCAAC	22	20	45.5	50.0	57.3	58.0	0.0	2.3	2.664963	93067	93691	570-786	1.000000	66.1
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	CGACCCAATGCTTTATTTCTGT	GAATGGTTAAAGCGCCCAAC	22	20	40.9	50.0	57.3	58.0	0.0	2.3	2.711157	93067	93691	589-805	1.000000	65.6
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	GTCCTAGTTGATCCTGATTCGA	GAATGGTTAAAGCGCCCAAC	22	20	45.5	50.0	57.1	58.0	0.0	2.3	2.929840	93067	93691	569-785	1.000000	64.0
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	TCCTAGTTGATCCTGATTCGAC	GAATGGTTAAAGCGCCCAAC	22	20	45.5	50.0	57.1	58.0	0.0	2.3	2.929840	93067	93691	568-784	1.000000	64.0
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	ACGACCAGTCAGTTAACAGC	GGGAAGCCGCATCTCTTTTT	20	20	50.0	50.0	57.8	58.8	8.6	0.0	0.972996	116861	117587	846-946	1.000000	76.6
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AGGATTCGAACCTACGACCA	GGGAAGCCGCATCTCTTTTT	20	20	50.0	50.0	58.2	58.8	15.0	0.0	0.979231	116861	117587	859-959	1.000000	76.5
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGATTCGAACCTACGACCAG	GGGAAGCCGCATCTCTTTTT	20	20	55.0	50.0	57.8	58.8	0.0	0.0	1.027906	116861	117587	858-958	1.000000	76.1
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	ACGACCAGTCAGTTAACAGC	AAATATGTAGGGGGAAGCCG	20	20	50.0	50.0	57.8	57.0	8.6	0.0	1.101626	116861	117587	857-957	1.000000	75.5
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	AGGATTCGAACCTACGACCA	AAATATGTAGGGGGAAGCCG	20	20	50.0	50.0	58.2	57.0	15.0	0.0	1.107861	116861	117587	870-970	1.000000	75.4
trnI-CAU-rpl23_p1	trnI-CAU-rpl23	1	GAATGGTTAAAGCGCCCAAC	CCTAGTTGATCCTGATTCGACA	20	22	50.0	45.5	58.0	57.3	2.3	0.0	2.664963	160937	161561	783-802	1.000000	63.3
trnI-CAU-rpl23_p2	trnI-CAU-rpl23	2	GAATGGTTAAAGCGCCCAAC	TGTCCTAGTTGATCCTGATTCG	20	22	50.0	45.5	58.0	57.3	2.3	0.0	2.664963	160937	161561	786-805	1.000000	63.3
trnI-CAU-rpl23_p3	trnI-CAU-rpl23	3	GAATGGTTAAAGCGCCCAAC	CGACCCAATGCTTTATTTCTGT	20	22	50.0	40.9	58.0	57.3	2.3	0.0	2.711157	160937	161561	805-824	1.000000	62.9
trnI-CAU-rpl23_p4	trnI-CAU-rpl23	4	GAATGGTTAAAGCGCCCAAC	GTCCTAGTTGATCCTGATTCGA	20	22	50.0	45.5	58.0	57.1	2.3	0.0	2.929840	160937	161561	785-804	1.000000	61.2
trnI-CAU-rpl23_p5	trnI-CAU-rpl23	5	GAATGGTTAAAGCGCCCAAC	TCCTAGTTGATCCTGATTCGAC	20	22	50.0	45.5	58.0	57.1	2.3	0.0	2.929840	160937	161561	784-803	1.000000	61.2