primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	95	383	406-406	1.000000	78.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	95	383	420-420	1.000000	78.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	95	383	419-419	1.000000	78.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	95	383	421-421	1.000000	78.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	95	383	356-356	1.000000	78.4
psbA_p1	psbA	1	TCAAGAGTGTTGATTATTGCTCC	AGAATTCGTGTGCTTGGGAG	23	20	39.1	50.0	56.9	58.2	0.0	0.0	4.325178	384	1445	1186-1186	1.000000	45.4
psbA_p2	psbA	2	TCAAGAGTGTTGATTATTGCTCC	AGAGAATTCGTGTGCTTGGG	23	20	39.1	50.0	56.9	58.2	0.0	0.0	4.325178	384	1445	1188-1188	1.000000	45.4
psbA_p3	psbA	3	TCAAGAGTGTTGATTATTGCTCC	GAGAATTCGTGTGCTTGGGA	23	20	39.1	50.0	56.9	58.2	0.0	0.0	4.330281	384	1445	1187-1187	1.000000	45.4
psbA_p4	psbA	4	CAAGAGTGTTGATTATTGCTCCT	AGAATTCGTGTGCTTGGGAG	23	20	39.1	50.0	56.9	58.2	0.0	0.0	4.332687	384	1445	1185-1185	1.000000	45.3
psbA_p5	psbA	5	CAAGAGTGTTGATTATTGCTCCT	AGAGAATTCGTGTGCTTGGG	23	20	39.1	50.0	56.9	58.2	0.0	0.0	4.332687	384	1445	1187-1187	1.000000	45.3
matK_p1	matK	1	CAACCCTTACTCTTCCTGCA	ACCCTGTTTTGACTATATCGCA	20	22	50.0	40.9	57.1	57.5	0.0	0.0	3.340533	2001	3503	1706-1706	1.000000	53.3
matK_p2	matK	2	ACCCTTACTCTTCCTGCATG	ACCCTGTTTTGACTATATCGCA	20	22	50.0	40.9	56.9	57.5	0.0	0.0	3.570326	2001	3503	1704-1704	1.000000	51.4
matK_p3	matK	3	TCAACCCTTACTCTTCCTGC	ACCCTGTTTTGACTATATCGCA	20	22	50.0	40.9	56.8	57.5	0.0	0.0	3.633500	2001	3503	1707-1707	1.000000	50.9
matK_p4	matK	4	CAACCCTTACTCTTCCTGCA	CCCTGTTTTGACTATATCGCAC	20	22	50.0	45.5	57.1	57.2	0.0	0.0	3.655135	2001	3503	1705-1705	1.000000	50.8
matK_p5	matK	5	AACCCTTACTCTTCCTGCATG	ACCCTGTTTTGACTATATCGCA	21	22	47.6	40.9	57.6	57.5	0.0	0.0	3.837897	2001	3503	1705-1705	1.000000	49.3
matK-trnK_p1	matK-trnK	1	AGGGGGTATAGGAAGTCGTG	AACGGTAGAGTACTCGGCTT	20	20	55.0	50.0	57.9	58.2	0.0	17.1	0.252048	3504	4241	827-827	1.000000	78.0
matK-trnK_p2	matK-trnK	2	AGGGGGTATAGGAAGTCGTG	ACGGTAGAGTACTCGGCTTT	20	20	55.0	50.0	57.9	58.2	0.0	17.1	0.252048	3504	4241	826-826	1.000000	78.0
matK-trnK_p3	matK-trnK	3	GAAGTCGTGGTGTCGAGATC	AACGGTAGAGTACTCGGCTT	20	20	55.0	50.0	58.1	58.2	0.0	17.1	0.270779	3504	4241	816-816	1.000000	77.8
matK-trnK_p4	matK-trnK	4	GAAGTCGTGGTGTCGAGATC	ACGGTAGAGTACTCGGCTTT	20	20	55.0	50.0	58.1	58.2	0.0	17.1	0.270779	3504	4241	815-815	1.000000	77.8
matK-trnK_p5	matK-trnK	5	ATAGGAAGTCGTGGTGTCGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.338141	3504	4241	819-819	1.000000	77.3
trnK-rps16_p1	trnK-rps16	1	AAAGCCGAGTACTCTACCGT	GCGCTCAACCTACAGAAACT	20	20	50.0	50.0	58.2	58.2	20.3	0.0	0.371395	4279	5104	949-949	1.000000	77.0
trnK-rps16_p2	trnK-rps16	2	AAGCCGAGTACTCTACCGTT	GCGCTCAACCTACAGAAACT	20	20	50.0	50.0	58.2	58.2	20.3	0.0	0.371395	4279	5104	948-948	1.000000	77.0
trnK-rps16_p3	trnK-rps16	3	AAAGCCGAGTACTCTACCGT	AAAAGGCGGGGGTTTTTACA	20	20	50.0	45.0	58.2	58.2	20.3	0.0	0.377602	4279	5104	913-913	1.000000	77.0
trnK-rps16_p4	trnK-rps16	4	AAGCCGAGTACTCTACCGTT	AAAAGGCGGGGGTTTTTACA	20	20	50.0	45.0	58.2	58.2	20.3	0.0	0.377602	4279	5104	912-912	1.000000	77.0
trnK-rps16_p5	trnK-rps16	5	AAGCCGAGTACTCTACCGTT	TTCTTGAAAAGGGCGCTCAA	20	20	50.0	45.0	58.2	58.3	20.3	11.4	0.482065	4279	5104	960-960	1.000000	76.1
rps16_p1	rps16	1	CCCACCCTCTTCTCTCTCTC	TCTCTGAAACGATCGAACAA	20	20	60.0	40.0	58.2	54.8	0.0	24.3	3.440137	5105	6227	1249-1249	1.000000	52.5
rps16_p2	rps16	2	CCCACCCTCTTCTCTCTCTC	TTCTCTGAAACGATCGAACA	20	20	60.0	40.0	58.2	54.8	0.0	24.3	3.440137	5105	6227	1250-1250	1.000000	52.5
rps16_p3	rps16	3	ATATGAATCACCCCCACCCT	TCTCTGAAACGATCGAACAA	20	20	50.0	40.0	57.8	54.8	0.0	24.3	3.444500	5105	6227	1261-1261	1.000000	52.4
rps16_p4	rps16	4	ATATGAATCACCCCCACCCT	TTCTCTGAAACGATCGAACA	20	20	50.0	40.0	57.8	54.8	0.0	24.3	3.444500	5105	6227	1262-1262	1.000000	52.4
rps16_p5	rps16	5	CCCACCCTCTTCTCTCTCTC	TCTGAAACGATCGAACAAATGAC	20	23	60.0	39.1	58.2	57.7	0.0	24.3	3.488675	5105	6227	1247-1247	1.000000	52.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	6228	7351	1192-1192	1.000000	77.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCTGTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.292817	6228	7351	1208-1208	1.000000	77.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.6	58.1	8.1	16.9	0.454608	6228	7351	1196-1196	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.5	58.1	8.1	16.9	0.531415	6228	7351	1198-1198	1.000000	75.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTCGCACGTTGCTTTTTACC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	8.1	16.9	0.684314	6228	7351	1195-1195	1.000000	74.5
trnS-GCU-FNM11_pgt005_p1	trnS-GCU-FNM11_pgt005	1	CAATCCGACGCTTTAGTCCA	TAGGAATCAAACCGAGGCAC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.401172	8713	9434	838-838	1.000000	76.8
trnS-GCU-FNM11_pgt005_p2	trnS-GCU-FNM11_pgt005	2	AATCCGACGCTTTAGTCCAC	TAGGAATCAAACCGAGGCAC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.402560	8713	9434	837-837	1.000000	76.8
trnS-GCU-FNM11_pgt005_p3	trnS-GCU-FNM11_pgt005	3	GCTCGTACAACGGATTAGCA	TAGGAATCAAACCGAGGCAC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.479317	8713	9434	856-856	1.000000	76.2
trnS-GCU-FNM11_pgt005_p4	trnS-GCU-FNM11_pgt005	4	ACGGAAAGAGAGGGATTCGA	TAGGAATCAAACCGAGGCAC	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.547938	8713	9434	892-892	1.000000	75.6
trnS-GCU-FNM11_pgt005_p5	trnS-GCU-FNM11_pgt005	5	AGCTCGTACAACGGATTAGC	TAGGAATCAAACCGAGGCAC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.601896	8713	9434	857-857	1.000000	75.2
FNM11_pgt005_p1	FNM11_pgt005	1	TTACGTCGGATTGCCTTGTT	ACTCATACTAATCTATAGCTTATTCT	20	26	45.0	26.9	57.8	53.0	0.0	0.0	11.183735	9435	10229	911-911	1.000000	40.0
FNM11_pgt005_p2	FNM11_pgt005	2	TACGTCGGATTGCCTTGTTC	ACTCATACTAATCTATAGCTTATTCT	20	26	50.0	26.9	58.3	53.0	0.0	0.0	11.293690	9435	10229	910-910	1.000000	40.0
FNM11_pgt005_p3	FNM11_pgt005	3	ATTACGTCGGATTGCCTTGT	ACTCATACTAATCTATAGCTTATTCT	20	26	45.0	26.9	57.6	53.0	0.0	0.0	11.407474	9435	10229	912-912	1.000000	40.0
FNM11_pgt005_p4	FNM11_pgt005	4	TGATTACGTCGGATTGCCTT	ACTCATACTAATCTATAGCTTATTCT	20	26	45.0	26.9	57.3	53.0	0.0	0.0	11.694886	9435	10229	914-914	1.000000	40.0
FNM11_pgt005_p5	FNM11_pgt005	5	TTGATTACGTCGGATTGCCT	ACTCATACTAATCTATAGCTTATTCT	20	26	45.0	26.9	57.3	53.0	0.0	0.0	11.694886	9435	10229	915-915	1.000000	40.0
rpoC2_p1	rpoC2	1	TGTGTCAGAAATTCCCCGAA	GGCATCTTGATACCACCAGG	20	20	45.0	55.0	57.1	58.0	0.0	0.0	0.968122	19282	19401	270-270	1.000000	72.6
rpoC2_p2	rpoC2	2	TTGTGTCAGAAATTCCCCGA	GGCATCTTGATACCACCAGG	20	20	45.0	55.0	57.1	58.0	0.0	0.0	0.968122	19282	19401	271-271	1.000000	72.6
rpoC2_p3	rpoC2	3	TGTGTCAGAAATTCCCCGAA	CGACACAGTGGCATCTTGAT	20	20	45.0	50.0	57.1	58.3	0.0	7.3	1.210493	19282	19401	279-279	1.000000	70.6
rpoC2_p4	rpoC2	4	TTGTGTCAGAAATTCCCCGA	CGACACAGTGGCATCTTGAT	20	20	45.0	50.0	57.1	58.3	0.0	7.3	1.210493	19282	19401	280-280	1.000000	70.6
rpoC2_p5	rpoC2	5	TTGTGTCAGAAATTCCCCGAA	GGCATCTTGATACCACCAGG	21	20	42.9	55.0	57.8	58.0	0.0	0.0	1.243315	19282	19401	271-271	1.000000	70.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCTTCATTTCCATCCCCGAG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.109256	27866	29011	1231-1231	1.000000	79.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCTTCATTTCCATCCCCGAG	TTGTTAATCAGGCGACACCC	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.211053	27866	29011	1249-1249	1.000000	78.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCTTCATTTCCATCCCCGAG	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.0	58.4	0.0	14.6	0.382619	27866	29011	1213-1213	1.000000	77.0
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCTTCATTTCCATCCCCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.491537	27866	29011	1232-1232	1.000000	76.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCCTTCATTTCCATCCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.517500	27866	29011	1233-1233	1.000000	75.9
ndhK-ndhC_p1	ndhK-ndhC	1	AGCGGCCATAAACTTGAGAG	ATGGCGAAAGGGAGCATTAG	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.056966	53115	53168	194-195	1.000000	80.0
ndhK-ndhC_p2	ndhK-ndhC	2	AGCGGCCATAAACTTGAGAG	GGCGAAAGGGAGCATTAGAA	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.068225	53115	53168	192-193	1.000000	79.9
ndhK-ndhC_p3	ndhK-ndhC	3	AGCGGCCATAAACTTGAGAG	TTTATGCATGGCGAAAGGGA	20	20	50.0	45.0	58.0	57.9	2.2	10.2	0.166137	53115	53168	201-202	1.000000	79.1
ndhK-ndhC_p4	ndhK-ndhC	4	AGCGGCCATAAACTTGAGAG	GTTTATGCATGGCGAAAGGG	20	20	50.0	50.0	58.0	57.8	2.2	10.2	0.238164	53115	53168	202-203	1.000000	78.5
ndhK-ndhC_p5	ndhK-ndhC	5	TTTGGGTTGTTCGAGCAAGT	ATGGCGAAAGGGAGCATTAG	20	20	45.0	50.0	58.2	58.0	15.5	0.0	0.269446	53115	53168	127-128	1.000000	78.5
accD_p1	accD	1	TGGAAATAGGGACAAGAAAACTCT	CGGATTTTGCTTTGATTCCGA	24	21	37.5	42.9	57.8	57.5	0.0	3.2	5.686826	60828	62303	1576-1576	1.000000	40.0
accD_p2	accD	2	TGGAAATAGGGACAAGAAAACTCT	TCGGATTTTGCTTTGATTCCG	24	21	37.5	42.9	57.8	57.5	0.0	2.9	5.686826	60828	62303	1577-1577	1.000000	40.0
accD_p3	accD	3	TGGAAATAGGGACAAGAAAACTC	CGGATTTTGCTTTGATTCCGA	23	21	39.1	42.9	56.4	57.5	0.0	3.2	6.042679	60828	62303	1576-1576	1.000000	40.0
accD_p4	accD	4	TGGAAATAGGGACAAGAAAACTC	TCGGATTTTGCTTTGATTCCG	23	21	39.1	42.9	56.4	57.5	0.0	2.9	6.042679	60828	62303	1577-1577	1.000000	40.0
accD_p5	accD	5	TGGAAATAGGGACAAGAAAACTCT	CGGATTTTGCTTTGATTCCG	24	20	37.5	45.0	57.8	56.0	0.0	0.0	6.188330	60828	62303	1576-1576	1.000000	40.0
accD-psaI_p1	accD-psaI	1	ACGCCTTTTTCCCCTTGAAT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	0.0	26.9	0.036685	62304	62956	761-761	1.000000	79.8
accD-psaI_p2	accD-psaI	2	GAAGCTTCACGCCTTTTTCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	21.7	26.9	0.078882	62304	62956	769-769	1.000000	79.5
accD-psaI_p3	accD-psaI	3	ACGCCTTTTTCCCCTTGAAT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.0	57.9	0.0	26.8	0.113219	62304	62956	767-767	1.000000	79.2
accD-psaI_p4	accD-psaI	4	GAAGCTTCACGCCTTTTTCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	21.7	26.8	0.155416	62304	62956	775-775	1.000000	78.9
accD-psaI_p5	accD-psaI	5	ACGCCTTTTTCCCCTTGAAT	GCCGGAAATACTAGGCCTAC	20	20	45.0	55.0	58.0	57.5	0.0	8.9	0.461977	62304	62956	750-750	1.000000	76.4