primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6065	7561	1382-1570	1.000000	83.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	AAAAATCGAAATGGGGCGTG	20	20	45.0	45.0	58.1	57.6	8.1	0.0	0.501493	6065	7561	1441-1629	1.000000	81.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6065	7561	1386-1574	1.000000	78.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6065	7561	1412-1600	1.000000	78.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6065	7561	1374-1562	1.000000	78.4
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AATCAGACGGAAAAGCGTCC	CTGAGGAAGCGCAATCTCTT	20	20	50.0	50.0	58.6	58.0	0.0	11.5	0.581676	10686	10807	277-279	1.000000	77.1
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AATCAGACGGAAAAGCGTCC	GGAGCAACTGGACCGTTTTA	20	20	50.0	50.0	58.6	58.1	0.0	0.0	0.673435	10686	10807	240-242	1.000000	76.6
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AATCAGACGGAAAAGCGTCC	GGACCGTTTTATCCTCGAGG	20	20	50.0	55.0	58.6	57.8	0.0	26.3	0.780800	10686	10807	231-233	1.000000	75.8
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	CGGAAAAGCGTCCATTGTCT	CTGAGGAAGCGCAATCTCTT	20	20	50.0	50.0	58.8	58.0	5.2	11.5	0.864619	10686	10807	270-272	1.000000	74.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AGACGGAAAAGCGTCCATTG	CTGAGGAAGCGCAATCTCTT	20	20	50.0	50.0	58.8	58.0	0.0	11.5	0.864619	10686	10807	273-275	1.000000	74.8
atpH-atpI_p1	atpH-atpI	1	CAAACCAGCAGCAATAACGG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	58.0	58.5	0.0	0.0	0.536469	14227	14931	806-1002	1.000000	79.4
atpH-atpI_p2	atpH-atpI	2	CCAAACCAGCAGCAATAACG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	58.0	58.5	0.0	0.0	0.536469	14227	14931	807-1003	1.000000	79.4
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	14227	14931	793-989	1.000000	79.3
atpH-atpI_p4	atpH-atpI	4	AAACCAGCAGCAATAACGGA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.1	58.5	0.0	0.0	0.613436	14227	14931	805-1001	1.000000	78.8
atpH-atpI_p5	atpH-atpI	5	AACCAGCAGCAATAACGGAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.1	58.5	0.0	0.0	0.613436	14227	14931	804-1000	1.000000	78.8
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	GCAGCAGGGTTTGAAAAAGG	GGTGGCTGAGTGGTTGATAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.160064	35737	36005	422-444	1.000000	81.3
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	GCAGCAGGGTTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.254503	35737	36005	406-428	1.000000	80.7
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TCGTAGGTCATTTGTGGCAC	GGTGGCTGAGTGGTTGATAG	20	20	50.0	55.0	58.2	58.0	11.6	0.0	0.226504	35737	36005	463-485	1.000000	80.6
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.1	58.2	0.0	17.2	0.347155	35737	36005	357-379	1.000000	80.3
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TCGTAGGTCATTTGTGGCAC	ATAGCCCCGGTCTTGAAAAC	20	20	50.0	50.0	58.2	57.9	11.6	0.0	0.320943	35737	36005	447-469	1.000000	79.9
psaA-ycf3_p1	psaA-ycf3	1	TTTAACTTCTGGTTCCGGCG	GAAGCGCACAATTGGTTGAA	20	20	50.0	45.0	58.5	57.9	0.0	11.5	0.617659	42279	43068	865-986	1.000000	79.2
psaA-ycf3_p2	psaA-ycf3	2	TTTAACTTCTGGTTCCGGCG	AAGCGCACAATTGGTTGAAG	20	20	50.0	45.0	58.5	57.9	0.0	11.5	0.623515	42279	43068	864-985	1.000000	79.2
psaA-ycf3_p3	psaA-ycf3	3	TTTAACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.5	57.8	0.0	0.0	0.648737	42279	43068	850-971	1.000000	79.0
psaA-ycf3_p4	psaA-ycf3	4	TTTAACTTCTGGTTCCGGCG	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.676508	42279	43068	854-975	1.000000	78.8
psaA-ycf3_p5	psaA-ycf3	5	CTGGTTCCGGCGAACTAATA	GAAGCGCACAATTGGTTGAA	20	20	50.0	45.0	57.4	57.9	9.3	11.5	0.722538	42279	43068	857-978	1.000000	78.4
ycf3_p1	ycf3	1	AAAACTAAAAGATGCCGGCT	ACAATTCTTTTCTTGCGCGG	20	20	40.0	45.0	55.9	57.9	29.7	8.8	2.131968	43069	45082	2107-2111	0.666667	52.0
ycf3_p2	ycf3	2	AAACTAAAAGATGCCGGCTT	ACAATTCTTTTCTTGCGCGG	20	20	40.0	45.0	55.9	57.9	29.7	8.8	2.131968	43069	45082	2106-2110	0.666667	52.0
ycf3_p3	ycf3	3	AACTAAAAGATGCCGGCTTT	ACAATTCTTTTCTTGCGCGG	20	20	40.0	45.0	55.9	57.9	29.7	8.8	2.131968	43069	45082	2105-2109	0.666667	52.0
ycf3_p4	ycf3	4	ACTAAAAGATGCCGGCTTTT	ACAATTCTTTTCTTGCGCGG	20	20	40.0	45.0	55.9	57.9	29.7	8.8	2.131968	43069	45082	2104-2108	0.666667	52.0
ycf3_p5	ycf3	5	ACTAAAAGATGCCGGCTTTT	GGGATCATTTTCTCGCGAGA	20	20	40.0	50.0	55.9	57.8	29.7	33.8	2.306559	43069	45082	2086-2090	0.666667	50.6
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCTCGTCA	20	24	50.0	33.3	58.1	55.1	0.0	19.7	7.013214	45473	45814	384-413	1.000000	47.0
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	GGCTTTTGTTTACCGAGGGT	AGTCGTAGAATATTATTCTCGTCA	20	24	50.0	33.3	58.4	55.1	0.0	19.7	7.306175	45473	45814	383-412	1.000000	47.0
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCTCGTCAA	20	25	50.0	32.0	58.1	55.8	0.0	19.7	7.327994	45473	45814	384-413	1.000000	47.0
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	AGGCTTTTGTTTACCGAGGG	GTCGTAGAATATTATTCTCGTCA	20	23	50.0	34.8	58.1	53.7	0.0	19.7	7.412373	45473	45814	383-412	1.000000	47.0
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	ATAGGAGAGATGGCCGAGTG	AGTCGTAGAATATTATTCTCGTCA	20	24	55.0	33.3	58.4	55.1	0.0	19.7	7.292165	45473	45814	434-463	1.000000	46.7
accD-psaI_p1	accD-psaI	1	TGACCTAATCGTCCCACGTA	TTGCAAGTGCCGGAAATACT	20	20	50.0	45.0	57.9	58.1	0.0	5.0	0.222984	58994	59658	832-911	1.000000	81.9
accD-psaI_p2	accD-psaI	2	TGACCTAATCGTCCCACGTA	AAAGAAGCCATTGCAAGTGC	20	20	50.0	45.0	57.9	57.8	0.0	13.5	0.286598	58994	59658	842-921	1.000000	81.4
accD-psaI_p3	accD-psaI	3	TTTGACCTAATCGTCCCACG	TTGCAAGTGCCGGAAATACT	20	20	50.0	45.0	57.6	58.1	0.0	5.0	0.469253	58994	59658	834-913	1.000000	79.9
accD-psaI_p4	accD-psaI	4	TTTGACCTAATCGTCCCACG	AAAGAAGCCATTGCAAGTGC	20	20	50.0	45.0	57.6	57.8	0.0	13.5	0.532868	58994	59658	844-923	1.000000	79.4
accD-psaI_p5	accD-psaI	5	TGACCTAATCGTCCCACGTA	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	57.9	57.5	0.0	8.9	0.579566	58994	59658	824-903	1.000000	79.1
psaI-ycf4_p1	psaI-ycf4	1	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	20	20	50.0	50.0	57.9	57.9	8.9	0.0	0.234974	59770	60241	584-625	1.000000	83.2
psaI-ycf4_p2	psaI-ycf4	2	AGTATTTCCGGCACTTGCAA	GGATAAAGGCCCAGCAGAAA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.239589	59770	60241	577-618	1.000000	83.1
psaI-ycf4_p3	psaI-ycf4	3	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	20	20	50.0	50.0	57.9	57.9	8.9	0.0	0.241071	59770	60241	587-628	1.000000	83.1
psaI-ycf4_p4	psaI-ycf4	4	AGTATTTCCGGCACTTGCAA	AAAGGATAAAGGCCCAGCAG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.245686	59770	60241	580-621	1.000000	83.1
psaI-ycf4_p5	psaI-ycf4	5	GCACTTGCAATGGCTTCTTT	GGATAAAGGCCCAGCAGAAA	20	20	45.0	50.0	57.8	57.9	13.5	0.0	0.303203	59770	60241	567-608	1.000000	82.7
petA-psbJ_p1	petA-psbJ	1	GTCTCCAAGGCCTTTTGGTC	ATTCCGGATTGGGTTCATCC	20	20	55.0	50.0	58.8	57.6	17.6	4.1	1.116486	63518	64592	1140-1211	1.000000	75.9
petA-psbJ_p2	petA-psbJ	2	TGGTCTTCTTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.0	57.6	0.0	4.1	1.322374	63518	64592	1125-1196	1.000000	74.2
petA-psbJ_p3	petA-psbJ	3	TTGGTCTTCTTGGCATCTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.0	57.6	0.0	4.1	1.322374	63518	64592	1126-1197	1.000000	74.2
petA-psbJ_p4	petA-psbJ	4	GCCTTTTGGTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	59.0	57.6	0.0	4.1	1.325116	63518	64592	1131-1202	1.000000	74.2
petA-psbJ_p5	petA-psbJ	5	TTGGTCTTCTTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	21	20	42.9	50.0	57.8	57.6	0.0	4.1	1.593161	63518	64592	1126-1197	1.000000	72.1
petB-petD_p1	petB-petD	1	CCGCTTCTTACTGCCGTATT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.389650	77094	77260	263-270	1.000000	81.1
petB-petD_p2	petB-petD	2	AAACAGGGTATTTCTGGCCC	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.557299	77094	77260	215-222	1.000000	80.6
petB-petD_p3	petB-petD	3	TATTGCCGCTTCTTACTGCC	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.661888	77094	77260	268-275	1.000000	78.8
petB-petD_p4	petB-petD	4	CCGCTTCTTACTGCCGTATT	AGTTCAAGTCACACACTCCC	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.686131	77094	77260	264-271	1.000000	78.7
petB-petD_p5	petB-petD	5	CCGCTTCTTACTGCCGTATT	GAATTCCAAGGCTAATGCGG	20	20	50.0	50.0	58.1	57.2	0.0	0.0	0.846013	77094	77260	313-320	1.000000	76.8
petD_p1	petD	1	AGTTGAAGGGAATTCTCTGAAGA	GTCACTTTGACGCGACTAGT	23	20	39.1	50.0	57.2	58.0	9.1	15.9	3.880218	77261	78477	1313-1357	1.000000	49.5
petD_p2	petD	2	AGTTGAAGGGAATTCTCTGAAGA	GACGCGACTAGTCCCTAGAT	23	20	39.1	55.0	57.2	58.1	9.1	15.9	3.950948	77261	78477	1305-1349	1.000000	48.9
petD_p3	petD	3	AGTTGAAGGGAATTCTCTGAAGA	TTTGACGCGACTAGTCCCTA	23	20	39.1	50.0	57.2	58.2	9.1	15.9	4.007449	77261	78477	1308-1352	1.000000	48.5
petD_p4	petD	4	AGTTGAAGGGAATTCTCTGAAGA	TCACTTTGACGCGACTAGTC	23	20	39.1	50.0	57.2	57.7	9.1	15.9	4.159620	77261	78477	1312-1356	1.000000	47.3
petD_p5	petD	5	AGTTGAAGGGAATTCTCTGAAGAA	GTCACTTTGACGCGACTAGT	24	20	37.5	50.0	57.8	58.0	9.1	15.9	4.240649	77261	78477	1313-1357	1.000000	46.6
rrn23_p1	rrn23	1	TCCATCCATTTAGCAGCACC	GCCGTGACCTTCTCTTGAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.157742	104878	107686	2980-2992	1.000000	79.6
rrn23_p2	rrn23	2	CCATCCATTTAGCAGCACCT	GCCGTGACCTTCTCTTGAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.163575	104878	107686	2979-2991	1.000000	79.6
rrn23_p3	rrn23	3	TCCATCCATTTAGCAGCACC	AAAACTTCTGTCGCTCCGTC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.558089	104878	107686	2956-2968	1.000000	76.4
rrn23_p4	rrn23	4	TCCATCCATTTAGCAGCACC	TCAAAACTTCTGTCGCTCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.559147	104878	107686	2958-2970	1.000000	76.4
rrn23_p5	rrn23	5	CCATCCATTTAGCAGCACCT	AAAACTTCTGTCGCTCCGTC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.563922	104878	107686	2955-2967	1.000000	76.4
ycf1_p1	ycf1	1	CGGAACTTCTTCGGAGTAGC	TTGATCGGCGTGTGATTGAT	20	20	55.0	45.0	58.1	58.0	0.0	6.8	0.112077	109539	110507	1476-1516	1.000000	84.1
ycf1_p2	ycf1	2	GTAACCCCCGGAACTTCTTC	TTGATCGGCGTGTGATTGAT	20	20	55.0	45.0	57.9	58.0	0.7	6.8	0.127771	109539	110507	1484-1524	1.000000	84.0
ycf1_p3	ycf1	3	AGGATTCGAACCTACGACCA	TTGATCGGCGTGTGATTGAT	20	20	50.0	45.0	58.2	58.0	15.0	6.8	0.179396	109539	110507	1622-1662	1.000000	83.2
ycf1_p4	ycf1	4	GGGAACGGGTTACCTACTCT	TTGATCGGCGTGTGATTGAT	20	20	55.0	45.0	58.1	58.0	5.5	6.8	0.164800	109539	110507	1720-1760	1.000000	83.1
ycf1_p5	ycf1	5	CGAGCCCCCTTTCTTTGATT	TTGATCGGCGTGTGATTGAT	20	20	50.0	45.0	58.2	58.0	0.0	6.8	0.183341	109539	110507	1688-1728	1.000000	83.0
ycf1-ndhF_p1	ycf1-ndhF	1	GGAAGAAGGGGTGGAAAGTG	TTGATCGGCGTGTGATTGAT	20	20	55.0	45.0	58.1	58.0	0.0	6.8	0.111707	110508	110602	479-512	1.000000	90.0
ycf1-ndhF_p2	ycf1-ndhF	2	AACCTCACAAGCGGAAGAAG	TTGATCGGCGTGTGATTGAT	20	20	50.0	45.0	58.1	58.0	0.0	6.8	0.138529	110508	110602	491-524	1.000000	89.6
ycf1-ndhF_p3	ycf1-ndhF	3	AGAAACCTCACAAGCGGAAG	TTGATCGGCGTGTGATTGAT	20	20	50.0	45.0	58.1	58.0	0.0	6.8	0.138529	110508	110602	494-527	1.000000	89.6
ycf1-ndhF_p4	ycf1-ndhF	4	GAAACCTCACAAGCGGAAGA	TTGATCGGCGTGTGATTGAT	20	20	50.0	45.0	58.1	58.0	0.0	6.8	0.143811	110508	110602	493-526	1.000000	89.5
ycf1-ndhF_p5	ycf1-ndhF	5	AAGAGGGATCCACCGAAGAA	TTGATCGGCGTGTGATTGAT	20	20	50.0	45.0	58.0	58.0	15.2	6.8	0.070143	110508	110602	399-432	0.666667	70.5
ccsA-ndhD_p1	ccsA-ndhD	1	TCCCACTCAGCAATTGTAGC	AGGTATTGGTATGTACCCCGA	20	21	50.0	47.6	57.9	57.7	7.4	6.7	1.447174	115251	115547	493-509	1.000000	71.0
ccsA-ndhD_p2	ccsA-ndhD	2	ACTCAGCAATTGTAGCGCTT	AGGTATTGGTATGTACCCCGA	20	21	45.0	47.6	58.2	57.7	18.7	6.7	1.519800	115251	115547	489-505	1.000000	70.4
ccsA-ndhD_p3	ccsA-ndhD	3	GGTTCCCACTCAGCAATTGT	AGGTATTGGTATGTACCCCGA	20	21	50.0	47.6	58.4	57.7	7.4	6.7	1.712491	115251	115547	496-512	1.000000	68.9
ccsA-ndhD_p4	ccsA-ndhD	4	TCCCACTCAGCAATTGTAGC	TCGGTTGATAAGGTCGAAGT	20	20	50.0	45.0	57.9	56.3	7.4	0.0	1.815776	115251	115547	456-472	1.000000	68.2
ccsA-ndhD_p5	ccsA-ndhD	5	ACTCAGCAATTGTAGCGCTT	TCGGTTGATAAGGTCGAAGT	20	20	45.0	45.0	58.2	56.3	18.7	0.0	1.888402	115251	115547	452-468	1.000000	67.7