primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpD_p1	atpD	1	TTTCTCAATTTGTTCACGGA	GGACCAAAAGAACGTGCAAC	20	20	35.0	50.0	53.4	58.2	0.0	0.0	4.810484	1463	2026	731-731	1.000000	46.1
atpD_p2	atpD	2	TTTCTCAATTTGTTCACGGA	ACAGTTGTTCGTGCACAAGA	20	20	35.0	45.0	53.4	58.3	0.0	18.6	4.912664	1463	2026	758-758	1.000000	45.1
atpD_p3	atpD	3	TTCTCAATTTGTTCACGGAT	GGACCAAAAGAACGTGCAAC	20	20	35.0	50.0	53.1	58.2	0.0	0.0	5.053432	1463	2026	730-730	1.000000	44.6
atpD_p4	atpD	4	ATTTCTCAATTTGTTCACGGA	GGACCAAAAGAACGTGCAAC	21	20	33.3	50.0	54.0	58.2	0.0	0.0	5.146726	1463	2026	732-732	1.000000	44.6
atpD_p5	atpD	5	TTTCTCAATTTGTTCACGGAT	GGACCAAAAGAACGTGCAAC	21	20	33.3	50.0	54.0	58.2	0.0	0.0	5.146726	1463	2026	731-731	1.000000	44.6
psaF_p1	psaF	1	ATTGTCCAAAATGCGCACTG	GGGCAAGTTAGCGTCTGAAT	20	20	45.0	50.0	57.9	58.3	14.9	0.0	0.329770	9767	10324	1876-1876	0.333333	50.7
psaF_p2	psaF	2	GTCCAAAATGCGCACTGAAA	GGGCAAGTTAGCGTCTGAAT	20	20	45.0	50.0	57.9	58.3	14.9	0.0	0.393673	9767	10324	1873-1873	0.333333	50.2
psaF_p3	psaF	3	GTTGAGCCCATTTCGCAAAA	GGGCAAGTTAGCGTCTGAAT	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.408369	9767	10324	1729-1729	0.333333	50.1
psaF_p4	psaF	4	TTGAGCCCATTTCGCAAAAC	GGGCAAGTTAGCGTCTGAAT	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.408369	9767	10324	1728-1728	0.333333	50.1
psaF_p5	psaF	5	TGATCGACGATCTGTTGAGC	GGGCAAGTTAGCGTCTGAAT	20	20	50.0	50.0	57.8	58.3	31.7	0.0	0.455733	9767	10324	1665-1665	0.333333	49.7
OOD24_pgt005-orf125a_p1	OOD24_pgt005-orf125a	1	ATCAGTGTAAGTGCTGCAGG	TTGCTGGCTTTCCTTGTGAT	20	20	50.0	45.0	57.9	58.0	17.1	0.0	0.105358	21284	22409	1503-1503	0.333333	52.5
OOD24_pgt005-orf125a_p2	OOD24_pgt005-orf125a	2	ATCAGTGTAAGTGCTGCAGG	TCGCTTACCACTATCGGCTA	20	20	50.0	50.0	57.9	58.0	17.1	0.0	0.133525	21284	22409	1592-1592	0.333333	52.3
OOD24_pgt005-orf125a_p3	OOD24_pgt005-orf125a	3	ATCAGTGTAAGTGCTGCAGG	GAAGATGGATCTGCTGCACA	20	20	50.0	50.0	57.9	58.0	17.1	0.0	0.139110	21284	22409	1804-1804	0.333333	52.2
OOD24_pgt005-orf125a_p4	OOD24_pgt005-orf125a	4	ATCAGTGTAAGTGCTGCAGG	GTGAAGATGGATCTGCTGCA	20	20	50.0	50.0	57.9	58.0	17.1	0.9	0.139110	21284	22409	1806-1806	0.333333	52.2
OOD24_pgt005-orf125a_p5	OOD24_pgt005-orf125a	5	ATCAGTGTAAGTGCTGCAGG	TGAAGATGGATCTGCTGCAC	20	20	50.0	50.0	57.9	58.0	17.1	0.0	0.139110	21284	22409	1805-1805	0.333333	52.2
OOD24_pgt007-OOD24_pgt008_p1	OOD24_pgt007-OOD24_pgt008	1	AACCTGTGACCTTCTGCTTG	AAAATGGGCCGAGTTGGATT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.089937	23231	23366	276-276	1.000000	87.9
OOD24_pgt007-OOD24_pgt008_p2	OOD24_pgt007-OOD24_pgt008	2	AACCTGTGACCTTCTGCTTG	GATTTACAATCCGCCCCCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094493	23231	23366	231-231	1.000000	87.9
OOD24_pgt007-OOD24_pgt008_p3	OOD24_pgt007-OOD24_pgt008	3	TTGAACCTGTGACCTTCTGC	GATTTACAATCCGCCCCCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.100047	23231	23366	234-234	1.000000	87.9
OOD24_pgt007-OOD24_pgt008_p4	OOD24_pgt007-OOD24_pgt008	4	TTGAACCTGTGACCTTCTGC	AAAATGGGCCGAGTTGGATT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.095490	23231	23366	279-279	1.000000	87.8
OOD24_pgt007-OOD24_pgt008_p5	OOD24_pgt007-OOD24_pgt008	5	AGACACTCTACCGCTGAGTT	AAAATGGGCCGAGTTGGATT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.150392	23231	23366	250-250	1.000000	87.4
rps2_p1	rps2	1	AACAAGAGAAACAGCCTGCA	AGTTATCTGTTTCCAAGCGGT	20	21	45.0	42.9	57.9	57.6	0.0	0.0	1.475821	53779	54468	1468-1468	0.333333	41.5
rps2_p2	rps2	2	AACAAGAGAAACAGCCTGCA	ACAACCCATTTTGCTTTTCGT	20	21	45.0	38.1	57.9	57.5	0.0	0.0	1.592417	53779	54468	1266-1266	0.333333	40.6
rps2_p3	rps2	3	AAGAGAAACAGCCTGCATGT	AGTTATCTGTTTCCAAGCGGT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.701829	53779	54468	1465-1465	0.333333	39.7
rps2_p4	rps2	4	ACAAGAGAAACAGCCTGCAT	AGTTATCTGTTTCCAAGCGGT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.701829	53779	54468	1467-1467	0.333333	39.7
rps2_p5	rps2	5	AGAGAAACAGCCTGCATGTT	AGTTATCTGTTTCCAAGCGGT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.701829	53779	54468	1464-1464	0.333333	39.7
rrf-psbY_p1	rrf-psbY	1	AACCACACTGATCCATTCCG	ATAATGCCCATGAAGCAGCA	20	20	50.0	45.0	57.9	57.9	0.0	14.5	0.190067	71759	72064	454-465	1.000000	90.5
rrf-psbY_p2	rrf-psbY	2	CGGAACCACACTGATCCATT	ATAATGCCCATGAAGCAGCA	20	20	50.0	45.0	57.9	57.9	0.0	14.5	0.190067	71759	72064	457-468	1.000000	90.5
rrf-psbY_p3	rrf-psbY	3	AACCACACTGATCCATTCCG	TAATGCCCATGAAGCAGCAA	20	20	50.0	45.0	57.9	58.2	0.0	14.5	0.263883	71759	72064	453-464	1.000000	89.9
rrf-psbY_p4	rrf-psbY	4	AACCACACTGATCCATTCCG	TGCCCATGAAGCAGCAATTA	20	20	50.0	45.0	57.9	58.2	0.0	6.3	0.263883	71759	72064	450-461	1.000000	89.9
rrf-psbY_p5	rrf-psbY	5	CGGAACCACACTGATCCATT	TGCCCATGAAGCAGCAATTA	20	20	50.0	45.0	57.9	58.2	0.0	6.3	0.263883	71759	72064	453-464	1.000000	89.9
thiG-ycf35_p1	thiG-ycf35	1	CCAGCACAAATGGCTAAAGC	GCAACCCTACCCAGTTGAAA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.010723	75003	76139	870-1435	0.666667	78.6
thiG-ycf35_p2	thiG-ycf35	2	ATACAGCAGGTGCACAAACA	GCAACCCTACCCAGTTGAAA	20	20	45.0	50.0	58.0	58.0	20.4	7.0	0.030439	75003	76139	1322-1887	0.666667	78.4
thiG-ycf35_p3	thiG-ycf35	3	ACCCAGTGAAGCAACTCTTG	GCAACCCTACCCAGTTGAAA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.037422	75003	76139	1514-1514	0.333333	53.0
thiG-ycf35_p4	thiG-ycf35	4	CACCCAGTGAAGCAACTCTT	GCAACCCTACCCAGTTGAAA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.037422	75003	76139	1515-1515	0.333333	53.0
thiG-ycf35_p5	thiG-ycf35	5	TATGGAAATTGGTGCCGAGG	GCAACCCTACCCAGTTGAAA	20	20	50.0	50.0	57.9	58.0	0.0	7.0	0.061628	75003	76139	1493-1493	0.333333	52.8
dnaK-rpl3_p1	dnaK-rpl3	1	TGTCGTACCTAAATCAATTCCT	ACAGCCACAATACTCCTCCA	22	20	36.4	50.0	54.9	58.3	0.0	0.0	5.427006	84618	84849	281-291	1.000000	51.1
dnaK-rpl3_p2	dnaK-rpl3	2	TGTCGTACCTAAATCAATTCCTACA	ACAGCCACAATACTCCTCCA	25	20	36.0	50.0	57.8	58.3	0.0	0.0	5.516280	84618	84849	281-291	1.000000	51.1
dnaK-rpl3_p3	dnaK-rpl3	3	TGTCGTACCTAAATCAATTCCT	CAGCCACAATACTCCTCCAA	22	20	36.4	50.0	54.9	57.2	0.0	0.0	5.871066	84618	84849	280-290	1.000000	51.1
dnaK-rpl3_p4	dnaK-rpl3	4	TTGTCGTACCTAAATCAATTCCT	ACAGCCACAATACTCCTCCA	23	20	34.8	50.0	55.7	58.3	0.0	0.0	5.668164	84618	84849	282-292	1.000000	51.0
dnaK-rpl3_p5	dnaK-rpl3	5	TGTCGTACCTAAATCAATTCCT	CCCCAATAAACCTACAGCCA	22	20	36.4	50.0	54.9	57.2	0.0	0.0	5.902344	84618	84849	294-304	1.000000	50.9
OOD24_pgt025-OOD24_pgt026_p1	OOD24_pgt025-OOD24_pgt026	1	CTTGAACCCTCACGCCTATC	GGTATGGCCAAGTGGTAAGG	20	20	55.0	55.0	58.1	57.9	0.0	11.0	0.111457	101011	101107	233-237	1.000000	88.5
OOD24_pgt025-OOD24_pgt026_p2	OOD24_pgt025-OOD24_pgt026	2	AACCCTCACGCCTATCACTA	GGTATGGCCAAGTGGTAAGG	20	20	50.0	55.0	57.8	57.9	0.0	11.0	0.207349	101011	101107	229-233	1.000000	87.7
OOD24_pgt025-OOD24_pgt026_p3	OOD24_pgt025-OOD24_pgt026	3	TAAGTCCGTCGTGTCTACCA	GGTATGGCCAAGTGGTAAGG	20	20	50.0	55.0	57.8	57.9	0.0	11.0	0.236260	101011	101107	197-201	1.000000	87.7
OOD24_pgt025-OOD24_pgt026_p4	OOD24_pgt025-OOD24_pgt026	4	ACCCTCACGCCTATCACTAG	GGTATGGCCAAGTGGTAAGG	20	20	55.0	55.0	58.3	57.9	0.0	11.0	0.359156	101011	101107	228-232	1.000000	86.5
OOD24_pgt025-OOD24_pgt026_p5	OOD24_pgt025-OOD24_pgt026	5	CTTGAACCCTCACGCCTATC	CGGTATGGCCAAGTGGTAAG	20	20	55.0	55.0	58.1	58.3	0.0	11.0	0.394980	101011	101107	234-238	1.000000	86.2
OOD24_pgt026-clpC_p1	OOD24_pgt026-clpC	1	CCTTACCACTTGGCCATACC	CCCATTCTACGGGCTTCTTC	20	20	55.0	55.0	57.9	58.0	6.1	0.0	0.097855	101179	101314	200-230	1.000000	91.2
OOD24_pgt026-clpC_p2	OOD24_pgt026-clpC	2	CTTACCACTTGGCCATACCG	CCCATTCTACGGGCTTCTTC	20	20	55.0	55.0	58.3	58.0	6.1	0.0	0.381378	101179	101314	199-229	1.000000	88.9
OOD24_pgt026-clpC_p3	OOD24_pgt026-clpC	3	TTGCAATCCACTGCCTTACC	CCCATTCTACGGGCTTCTTC	20	20	50.0	55.0	58.5	58.0	0.0	0.0	0.500349	101179	101314	213-243	1.000000	88.0
OOD24_pgt026-clpC_p4	OOD24_pgt026-clpC	4	ATCCACTGCCTTACCACTTG	CCCATTCTACGGGCTTCTTC	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.541972	101179	101314	208-238	1.000000	87.7
OOD24_pgt026-clpC_p5	OOD24_pgt026-clpC	5	CAATCCACTGCCTTACCACT	CCCATTCTACGGGCTTCTTC	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.541972	101179	101314	210-240	1.000000	87.7
clpC-psbA_p1	clpC-psbA	1	AACCGCTTCCAGAACCAATT	TTCTCAGCTATGCACGGTTC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.014769	104081	105189	1687-2209	0.666667	78.5
clpC-psbA_p2	clpC-psbA	2	AACCGCTTCCAGAACCAATT	ATTCCAAGCTGAGCACAACA	20	20	45.0	45.0	58.0	58.0	0.0	7.4	0.019151	104081	105189	1763-2285	0.666667	78.5
clpC-psbA_p3	clpC-psbA	3	AACCGCTTCCAGAACCAATT	TCCAAGCTGAGCACAACATT	20	20	45.0	45.0	58.0	58.0	0.0	7.4	0.019151	104081	105189	1761-2283	0.666667	78.5
clpC-psbA_p4	clpC-psbA	4	AACCGCTTCCAGAACCAATT	TTCCAAGCTGAGCACAACAT	20	20	45.0	45.0	58.0	58.0	0.0	7.4	0.019151	104081	105189	1762-2284	0.666667	78.5
clpC-psbA_p5	clpC-psbA	5	GGCTGAGCAGTGTTTATCGA	TTCTCAGCTATGCACGGTTC	20	20	50.0	50.0	58.0	58.0	6.8	0.0	0.020290	104081	105189	1887-1887	0.333333	53.2
rbcR_p1	rbcR	1	TGTCCTTGTTTCTTGCGTGT	TGCTGCTTCATGGGCATTAT	20	20	45.0	45.0	58.3	57.9	0.0	13.0	0.330975	112753	113676	1780-1811	0.666667	70.9
rbcR_p2	rbcR	2	TGTCCTTGTTTCTTGCGTGT	TAATTGCTGCTTCATGGGCA	20	20	45.0	45.0	58.3	58.2	0.0	12.3	0.404791	112753	113676	1784-1815	0.666667	70.3
rbcR_p3	rbcR	3	TGTCCTTGTTTCTTGCGTGT	TTGCTGCTTCATGGGCATTA	20	20	45.0	45.0	58.3	58.2	0.0	13.0	0.404791	112753	113676	1781-1812	0.666667	70.3
rbcR_p4	rbcR	4	TGTCCTTGTTTCTTGCGTGT	GGAGTTCAAGCAGCTCAGAA	20	20	45.0	50.0	58.3	57.8	0.0	0.0	0.430646	112753	113676	1565-1565	0.333333	49.9
rbcR_p5	rbcR	5	TGTCCTTGTTTCTTGCGTGT	AAGGAGTTCAAGCAGCTCAG	20	20	45.0	50.0	58.3	57.8	0.0	0.0	0.436722	112753	113676	1567-1567	0.333333	49.8
psbY-rrf_p1	psbY-rrf	1	ATAATGCCCATGAAGCAGCA	AACCACACTGATCCATTCCG	20	20	45.0	50.0	57.9	57.9	14.5	0.0	0.190067	114257	114562	454-465	1.000000	90.5
psbY-rrf_p2	psbY-rrf	2	ATAATGCCCATGAAGCAGCA	CGGAACCACACTGATCCATT	20	20	45.0	50.0	57.9	57.9	14.5	0.0	0.190067	114257	114562	457-468	1.000000	90.5
psbY-rrf_p3	psbY-rrf	3	TAATGCCCATGAAGCAGCAA	AACCACACTGATCCATTCCG	20	20	45.0	50.0	58.2	57.9	14.5	0.0	0.263883	114257	114562	453-464	1.000000	89.9
psbY-rrf_p4	psbY-rrf	4	TGCCCATGAAGCAGCAATTA	AACCACACTGATCCATTCCG	20	20	45.0	50.0	58.2	57.9	6.3	0.0	0.263883	114257	114562	450-461	1.000000	89.9
psbY-rrf_p5	psbY-rrf	5	TGCCCATGAAGCAGCAATTA	CGGAACCACACTGATCCATT	20	20	45.0	50.0	58.2	57.9	6.3	0.0	0.263883	114257	114562	453-464	1.000000	89.9