primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCCCTCGCTTCATCCAAATG	AAAGGCGGAGGTTTTTAAGT	20	20	50.0	40.0	58.0	55.5	0.0	0.0	2.588179	6529	7129	640-995	1.000000	71.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AAAGGCGGAGGTTTTTAAGT	20	20	55.0	40.0	57.9	55.5	0.0	0.0	2.625779	6529	7129	657-1012	1.000000	71.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGATGGTATGGACGAATCCC	AAAGGCGGAGGTTTTTAAGT	20	20	55.0	40.0	57.9	55.5	0.0	0.0	2.625779	6529	7129	656-1011	1.000000	71.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAAGGCGGAGGTTTTTAAGT	20	20	50.0	40.0	58.2	55.5	20.3	0.0	2.713913	6529	7129	596-951	1.000000	70.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAAGGCGGAGGTTTTTAAGT	20	20	50.0	40.0	58.2	55.5	20.3	0.0	2.713913	6529	7129	595-950	1.000000	70.3
rpoC2-rpoC1_p1	rpoC2-rpoC1	1	CCCGTTCTGCCATAAGTACC	GGGTTTTGTCAGGCCTGTTA	20	20	55.0	50.0	58.1	58.0	0.0	31.6	0.060828	23549	23759	257-298	1.000000	84.9
rpoC2-rpoC1_p2	rpoC2-rpoC1	2	GCGGTTCCGTCTATCACTTT	GGGTTTTGTCAGGCCTGTTA	20	20	50.0	50.0	58.0	58.0	12.8	31.6	0.016264	23549	23759	297-338	1.000000	84.6
rpoC2-rpoC1_p3	rpoC2-rpoC1	3	TGTGAAAGACCAGATTGGCC	GGGTTTTGTCAGGCCTGTTA	20	20	50.0	50.0	58.1	58.0	0.0	31.6	0.100594	23549	23759	275-316	1.000000	84.2
rpoC2-rpoC1_p4	rpoC2-rpoC1	4	TAAGTCGTTTCATGGCGGTT	GGGTTTTGTCAGGCCTGTTA	20	20	45.0	50.0	57.8	58.0	0.0	31.6	0.183145	23549	23759	311-352	1.000000	83.0
rpoC2-rpoC1_p5	rpoC2-rpoC1	5	GCGGTTCCGTCTATCACTTT	ATACAGGGGTTTTGTCAGGC	20	20	50.0	50.0	58.0	57.8	12.8	0.0	0.221913	23549	23759	303-344	1.000000	82.8
petN-psbM_p1	petN-psbM	1	AGTCCCTTTTTGACTCTGCG	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.190493	31897	33085	1344-1859	1.000000	85.0
petN-psbM_p2	petN-psbM	2	AAAGGGATTGTGCATTCGGT	AGTATCCCCTTCTGACTCCG	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.151373	31897	33085	1864-2381	1.000000	84.9
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.226077	31897	33085	1246-1761	1.000000	84.8
petN-psbM_p4	petN-psbM	4	TCCACGGTAGCATAAAGGGA	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.206016	31897	33085	1877-2394	1.000000	84.5
petN-psbM_p5	petN-psbM	5	CCGTTGGATAGACAGACACG	AGTATCCCCTTCTGACTCCG	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.165353	31897	33085	2095-2219	0.428571	61.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	34319	35051	688-861	1.000000	89.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	34319	35051	653-826	1.000000	88.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	34319	35051	685-858	1.000000	88.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.452638	34319	35051	690-863	1.000000	87.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.4	58.0	0.0	0.0	0.561703	34319	35051	723-896	1.000000	86.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	ACAGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	58.1	58.1	0.0	9.5	0.141958	35124	36577	1613-1637	1.000000	83.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	57.9	58.1	0.0	9.5	0.156553	35124	36577	1609-1633	1.000000	83.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	ACAGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	58.1	57.8	0.0	9.5	0.245286	35124	36577	1620-1644	1.000000	83.0
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	57.9	57.8	0.0	9.5	0.259880	35124	36577	1616-1640	1.000000	82.9
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	ACAGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	20	20	50.0	50.0	58.1	58.2	0.0	9.5	0.294228	35124	36577	1621-1645	1.000000	82.6
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	48290	49082	895-1054	1.000000	72.9
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	48290	49082	896-1055	1.000000	72.9
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	48290	49082	916-1075	1.000000	72.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	48290	49082	917-1076	1.000000	72.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.776834	48290	49082	866-1025	1.000000	72.2
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	ACTTCCGACTCTAGGCCTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	12.6	0.0	0.192760	50099	50437	485-512	1.000000	81.2
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	ACTTCCGACTCTAGGCCTTT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.1	57.8	12.6	0.0	0.300513	50099	50437	453-480	1.000000	80.5
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	ACTTCCGACTCTAGGCCTTT	TCGATTATGTGAGCAGCCTG	20	20	50.0	50.0	58.1	57.8	12.6	0.0	0.289695	50099	50437	510-537	1.000000	80.3
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.7	57.9	0.0	0.0	0.439081	50099	50437	460-487	1.000000	79.4
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GTTAGTCCCGGTAAAGCTCC	TCGATTATGTGAGCAGCCTG	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.536017	50099	50437	485-512	1.000000	78.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	22	20	31.8	55.0	53.9	58.1	0.0	0.0	6.230058	54405	55669	1046-1324	1.000000	48.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	24	20	33.3	55.0	56.3	58.1	0.0	0.0	5.764085	54405	55669	1095-1373	1.000000	48.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCAAAATAGGAATAAGACTTG	CCGAGAAAGTCTACGGTTCG	23	20	34.8	55.0	54.9	58.1	0.0	0.0	6.163964	54405	55669	1095-1373	1.000000	48.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ATGCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	25	20	32.0	55.0	56.8	58.1	0.0	0.0	6.305861	54405	55669	1096-1374	1.000000	48.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAAGACTTGAT	CCGAGAAAGTCTACGGTTCG	25	20	32.0	55.0	56.8	58.1	0.0	0.0	6.305861	54405	55669	1095-1373	1.000000	48.7
accD-psaI_p1	accD-psaI	1	TCCACGCTTTCTTTCCTTTGA	TGGACTTATTCATCTTTCTACATGA	21	25	42.9	32.0	58.1	55.8	0.0	0.0	8.268942	62946	63435	570-584	0.857143	36.7
accD-psaI_p2	accD-psaI	2	GCTCCACGCTTTCTTTCCTT	TGGACTTATTCATCTTTCTACATGA	20	25	50.0	32.0	58.8	55.8	0.0	0.0	7.955319	62946	63435	572-586	0.857143	36.6
accD-psaI_p3	accD-psaI	3	GCTCCACGCTTTCTTTCCT	TGGACTTATTCATCTTTCTACATGA	19	25	52.6	32.0	58.1	55.8	0.0	0.0	8.260583	62946	63435	572-586	0.857143	36.6
accD-psaI_p4	accD-psaI	4	GCTCCACGCTTTCTTTCCTT	TCTAAGTGAGTATAGAATGGACT	20	23	50.0	34.8	58.8	53.5	0.0	0.0	8.271604	62946	63435	589-603	0.857143	36.6
accD-psaI_p5	accD-psaI	5	GAGTTATTTCAGCTCCACGC	TGGACTTATTCATCTTTCTACATGA	20	25	50.0	32.0	56.9	55.8	0.0	0.0	8.290281	62946	63435	583-597	0.857143	36.6
ycf4-cemA_p1	ycf4-cemA	1	AATCGAACAAAAAGCTGCCG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.127121	64540	65382	865-998	1.000000	85.5
ycf4-cemA_p2	ycf4-cemA	2	CGAACAAAAAGCTGCCGAAT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.127121	64540	65382	862-995	1.000000	85.5
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	64540	65382	843-976	1.000000	85.2
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCTGCCGAATTGG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	64540	65382	859-992	1.000000	84.7
ycf4-cemA_p5	ycf4-cemA	5	TTGCGTGTACCGATTGAAGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	7.8	0.0	0.234478	64540	65382	831-964	1.000000	84.7
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTTATTTTGGCACA	GCAGGTATTCTTGTGATCGGT	21	21	42.9	47.6	57.4	58.1	0.0	0.0	2.684842	67283	68262	1047-1217	1.000000	64.4
petA-psbJ_p2	petA-psbJ	2	TGGCATCTGTTATTTTGGCAC	GCAGGTATTCTTGTGATCGGT	21	21	42.9	47.6	57.4	58.1	0.0	0.0	2.684842	67283	68262	1048-1218	1.000000	64.4
petA-psbJ_p3	petA-psbJ	3	GGCATCTGTTATTTTGGCACA	AGCAGGTATTCTTGTGATCGG	21	21	42.9	47.6	57.4	57.8	0.0	0.0	2.776442	67283	68262	1048-1218	1.000000	63.7
petA-psbJ_p4	petA-psbJ	4	TGGCATCTGTTATTTTGGCAC	AGCAGGTATTCTTGTGATCGG	21	21	42.9	47.6	57.4	57.8	0.0	0.0	2.776442	67283	68262	1049-1219	1.000000	63.7
petA-psbJ_p5	petA-psbJ	5	TTGGCATCTGTTATTTTGGCAC	GCAGGTATTCTTGTGATCGGT	22	21	40.9	47.6	58.1	58.1	0.0	0.0	3.169502	67283	68262	1049-1219	1.000000	60.5
ndhF-rpl32_p1	ndhF-rpl32	1	TGCCGACGGAAAAAGAAGAA	CCCAATATCCTTTCCCTTTCCA	20	22	45.0	45.5	58.0	57.7	20.5	0.0	2.308076	117325	118425	1168-1368	1.000000	70.0
ndhF-rpl32_p2	ndhF-rpl32	2	TTGCCGACGGAAAAAGAAGA	CCCAATATCCTTTCCCTTTCCA	20	22	45.0	45.5	58.0	57.7	20.5	0.0	2.308076	117325	118425	1169-1369	1.000000	70.0
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGAAGAAGTCCCGCTCC	CCCAATATCCTTTCCCTTTCCA	20	22	50.0	45.5	57.8	57.7	0.0	0.0	2.450905	117325	118425	1158-1358	1.000000	68.9
ndhF-rpl32_p4	ndhF-rpl32	4	AAGAAGAAGTCCCGCTCCTA	CCCAATATCCTTTCCCTTTCCA	20	22	50.0	45.5	57.8	57.7	0.0	0.0	2.497678	117325	118425	1156-1356	1.000000	68.5
ndhF-rpl32_p5	ndhF-rpl32	5	AGAAGAAGTCCCGCTCCTAT	CCCAATATCCTTTCCCTTTCCA	20	22	50.0	45.5	57.5	57.7	0.0	0.0	2.727419	117325	118425	1155-1355	1.000000	66.7
ndhG-ndhI_p1	ndhG-ndhI	1	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.087551	124088	124498	1000-1069	1.000000	84.4
ndhG-ndhI_p2	ndhG-ndhI	2	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.087551	124088	124498	1001-1070	1.000000	84.4
ndhG-ndhI_p3	ndhG-ndhI	3	CATTTATGGCCCCCACGTAA	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	57.9	58.1	0.0	1.0	0.111946	124088	124498	1080-1149	1.000000	83.8
ndhG-ndhI_p4	ndhG-ndhI	4	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.070613	124088	124498	1382-1451	1.000000	83.2
ndhG-ndhI_p5	ndhG-ndhI	5	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.070613	124088	124498	1381-1450	1.000000	83.2
trnA-UGC_p1	trnA-UGC	1	CATAGATCGAACCGCCGAAT	GCATCTGACTCCTTCATGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.100097	139558	140447	993-1047	1.000000	84.2
trnA-UGC_p2	trnA-UGC	2	CATAGATCGAACCGCCGAAT	TCTGACTCCTTCATGCATGC	20	20	50.0	50.0	57.9	58.0	0.0	22.2	0.100097	139558	140447	990-1044	1.000000	84.2
trnA-UGC_p3	trnA-UGC	3	CATAGATCGAACCGCCGAAT	TGACTCCTTCATGCATGCTC	20	20	50.0	50.0	57.9	58.0	0.0	22.2	0.100097	139558	140447	988-1042	1.000000	84.2
trnA-UGC_p4	trnA-UGC	4	AATCATAGATCGAACCGCCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	139558	140447	1071-1125	1.000000	84.1
trnA-UGC_p5	trnA-UGC	5	CATAGATCGAACCGCCGAAT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	139558	140447	1068-1122	1.000000	84.1
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	149961	150642	826-861	1.000000	83.3
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	149961	150642	825-860	1.000000	83.3
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	149961	150642	828-863	1.000000	83.3
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	149961	150642	827-862	1.000000	83.3
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	20	20	50.0	50.0	58.0	58.2	15.9	0.7	0.240071	149961	150642	829-864	1.000000	83.3