primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	76	304	297-299	1.000000	84.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	76	304	311-313	1.000000	84.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	76	304	310-312	1.000000	84.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	76	304	312-314	1.000000	84.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.5	57.9	6.4	0.0	0.554952	76	304	326-328	1.000000	81.2
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAATAGG	20	24	50.0	37.5	58.0	57.9	0.0	0.0	4.172656	9549	9671	260-264	1.000000	50.6
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	TGGAAATAAATTGCGTCCAATAGG	20	24	55.0	37.5	58.1	57.9	0.0	0.0	4.201493	9549	9671	263-267	1.000000	50.3
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAA	20	20	50.0	35.0	58.0	53.8	0.0	0.0	4.269330	9549	9671	260-264	1.000000	49.8
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	TGGAAATAAATTGCGTCCAA	20	20	55.0	35.0	58.1	53.8	0.0	0.0	4.298167	9549	9671	263-267	1.000000	49.5
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.404255	9549	9671	219-223	1.000000	49.5
petN-psbM_p1	petN-psbM	1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTCAGCCAAAATGA	20	20	50.0	40.0	58.3	56.4	0.0	0.0	1.818304	28173	28871	807-821	1.000000	68.8
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGCCAAAATGA	20	20	50.0	40.0	57.6	56.4	0.0	0.0	1.908233	28173	28871	745-759	1.000000	68.4
petN-psbM_p3	petN-psbM	3	ATTGTAAGTCTCGCTTGGGC	AAACGGTCAGCCAAAATGAT	20	20	50.0	40.0	58.3	56.2	0.0	0.0	2.049275	28173	28871	806-820	1.000000	67.0
petN-psbM_p4	petN-psbM	4	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAATGATT	20	20	50.0	40.0	58.3	56.2	0.0	0.0	2.049275	28173	28871	805-819	1.000000	67.0
petN-psbM_p5	petN-psbM	5	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAATGA	20	21	50.0	38.1	58.3	57.2	0.0	0.0	2.071244	28173	28871	808-822	1.000000	66.8
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	35953	36270	463-475	1.000000	88.4
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	35953	36270	461-473	1.000000	88.0
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	0.0	1.1	1.036941	35953	36270	457-469	1.000000	83.2
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.9	57.8	0.0	1.1	1.102537	35953	36270	462-474	1.000000	82.6
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	35953	36270	458-470	1.000000	80.8
trnG-UCC-trnfM-CAU_p1	trnG-UCC-trnfM-CAU	1	TCTTTGCCAAGGAGAAGACG	GTTCAAATCCTGTCTCCGCA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.361422	36341	36570	295-296	1.000000	82.8
trnG-UCC-trnfM-CAU_p2	trnG-UCC-trnfM-CAU	2	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.394901	36341	36570	328-329	1.000000	82.0
trnG-UCC-trnfM-CAU_p3	trnG-UCC-trnfM-CAU	3	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.394901	36341	36570	329-330	1.000000	81.9
trnG-UCC-trnfM-CAU_p4	trnG-UCC-trnfM-CAU	4	TCTTTGCCAAGGAGAAGACG	TCAAATCCTGTCTCCGCAAA	20	20	50.0	45.0	57.8	57.4	0.0	0.0	0.729530	36341	36570	293-294	1.000000	79.9
trnG-UCC-trnfM-CAU_p5	trnG-UCC-trnfM-CAU	5	TCTTTGCCAAGGAGAAGACG	TTCAAATCCTGTCTCCGCAA	20	20	50.0	45.0	57.8	57.4	0.0	0.0	0.729530	36341	36570	294-295	1.000000	79.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	46637	47308	726-745	1.000000	84.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	46637	47308	758-777	1.000000	83.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46637	47308	714-733	1.000000	82.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	46637	47308	725-744	1.000000	82.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46637	47308	746-765	1.000000	81.9
trnL-UAA_p1	trnL-UAA	1	AGGACATTACACTGAGATCCT	ACCTTCAAACCATTAATGAAAA	21	22	42.9	27.3	55.5	52.4	0.0	17.2	11.092606	47309	47886	682-686	1.000000	45.4
trnL-UAA_p2	trnL-UAA	2	GAAGGACATTACACTGAGATCCT	ACCTTCAAACCATTAATGAAAA	23	22	43.5	27.3	57.4	52.4	0.0	17.2	11.185306	47309	47886	684-688	1.000000	45.4
trnL-UAA_p3	trnL-UAA	3	AGGACATTACACTGAGATCCT	ACCTTCAAACCATTAATGAAAAA	21	23	42.9	26.1	55.5	53.3	0.0	17.2	11.220809	47309	47886	682-686	1.000000	45.4
trnL-UAA_p4	trnL-UAA	4	GAAGGACATTACACTGAGATCCT	ACCTTCAAACCATTAATGAAAAA	23	23	43.5	26.1	57.4	53.3	0.0	17.2	11.313508	47309	47886	684-688	1.000000	45.4
trnL-UAA_p5	trnL-UAA	5	AAGGACATTACACTGAGATCCT	ACCTTCAAACCATTAATGAAAA	22	22	40.9	27.3	56.3	52.4	0.0	17.2	11.315889	47309	47886	683-687	1.000000	45.4
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	47887	48241	445-450	1.000000	78.3
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	AAAGATGTGCCAGGAACCAG	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.559269	47887	48241	466-471	1.000000	77.9
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.564710	47887	48241	460-465	1.000000	77.9
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	GATGTGCCAGGAACCAGATT	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.609199	47887	48241	463-468	1.000000	77.5
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	AAAAGATGTGCCAGGAACCA	20	20	50.0	45.0	57.5	57.6	0.0	0.0	0.854474	47887	48241	467-472	1.000000	75.5
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	AGAGTCATTGGTTCAAATCCA	CTCTCAGACGAGCTAGGACA	21	20	38.1	55.0	55.1	58.0	0.0	0.0	3.928687	52152	52416	346-357	1.000000	55.4
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	AGAGTCATTGGTTCAAATCCA	ACTCTCAGACGAGCTAGGAC	21	20	38.1	55.0	55.1	58.0	0.0	0.0	3.930109	52152	52416	347-358	1.000000	55.4
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	AGAGTCATTGGTTCAAATCCA	CGAGCTAGGACACGAGTAGA	21	20	38.1	55.0	55.1	58.1	0.0	0.0	3.960151	52152	52416	338-349	1.000000	55.3
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	AGAGTCATTGGTTCAAATCCAA	CTCTCAGACGAGCTAGGACA	22	20	36.4	55.0	55.9	58.0	0.0	0.0	4.140010	52152	52416	346-357	1.000000	53.7
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	AGAGTCATTGGTTCAAATCCAA	ACTCTCAGACGAGCTAGGAC	22	20	36.4	55.0	55.9	58.0	0.0	0.0	4.141432	52152	52416	347-358	1.000000	53.7
accD-psaI_p1	accD-psaI	1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	0.0	26.9	0.032087	58685	59273	854-863	1.000000	84.0
accD-psaI_p2	accD-psaI	2	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	14.2	26.9	0.034834	58685	59273	1024-1033	1.000000	83.4
accD-psaI_p3	accD-psaI	3	AACGCCTACATTGCATTTGC	CATGGGGGCTCGTTCATTTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.056418	58685	59273	1018-1027	1.000000	83.4
accD-psaI_p4	accD-psaI	4	AACGCCTACATTGCATTTGC	TGGGGGCTCGTTCATTTATG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.056418	58685	59273	1016-1025	1.000000	83.4
accD-psaI_p5	accD-psaI	5	AACGCCTACATTGCATTTGC	GGGGGCTCGTTCATTTATGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.057866	58685	59273	1015-1024	1.000000	83.4
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GGAGGTAATAGTTAAAGCCGCT	20	22	45.0	45.5	58.0	57.9	0.0	0.0	2.086640	64316	65618	1468-1475	1.000000	67.7
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	AGCGGAGGTAATAGTTAAAGCC	20	22	45.0	45.5	58.0	57.9	0.0	0.0	2.086640	64316	65618	1471-1478	1.000000	67.6
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AGCGGAGGTAATAGTTAAAGCC	20	22	50.0	45.5	57.9	57.9	6.4	0.0	2.120635	64316	65618	1460-1467	1.000000	67.4
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GGAGGTAATAGTTAAAGCCGCT	20	22	50.0	45.5	57.9	57.9	6.4	0.0	2.120635	64316	65618	1457-1464	1.000000	67.4
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	GGAGGTAATAGTTAAAGCCGCT	20	22	50.0	45.5	57.8	57.9	24.9	0.0	2.225643	64316	65618	1380-1387	1.000000	66.8
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.138141	66447	66903	544-547	1.000000	84.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.139485	66447	66903	543-546	1.000000	84.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.146177	66447	66903	555-558	1.000000	84.4
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147511	66447	66903	554-557	1.000000	84.4
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	GGTGCCACGGAAAGATATGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147511	66447	66903	557-560	1.000000	84.4
psbB-psbT_p1	psbB-psbT	1	GTAGAATTTGGCGCATTCCA	GTTTGAATTTTCGGGGGTTCC	20	21	45.0	47.6	56.8	58.0	10.8	0.0	2.259133	73813	73988	323-328	1.000000	65.4
psbB-psbT_p2	psbB-psbT	2	AGTAGAATTTGGCGCATTCCA	GTTTGAATTTTCGGGGGTTCC	21	21	42.9	47.6	58.3	58.0	10.8	0.0	2.312627	73813	73988	324-329	1.000000	64.9
psbB-psbT_p3	psbB-psbT	3	GTAGAATTTGGCGCATTCCAA	GTTTGAATTTTCGGGGGTTCC	21	21	42.9	47.6	57.5	58.0	10.8	0.0	2.536912	73813	73988	323-328	1.000000	63.1
psbB-psbT_p4	psbB-psbT	4	AGTAGAATTTGGCGCATTCC	GTTTGAATTTTCGGGGGTTCC	20	21	45.0	47.6	56.5	58.0	10.8	0.0	2.556154	73813	73988	324-329	1.000000	63.0
psbB-psbT_p5	psbB-psbT	5	GTTCAAGTAGAATTTGGCGCA	GTTTGAATTTTCGGGGGTTCC	21	21	42.9	47.6	57.4	58.0	10.8	0.0	2.587324	73813	73988	329-334	1.000000	62.7
rps11-rpl36_p1	rps11-rpl36	1	TTTACGTGCACTTTTACGCG	AAAATGTCGACTGATCCGCA	20	20	45.0	45.0	57.7	57.9	16.2	17.9	0.375587	79268	79423	303-307	1.000000	80.7
rps11-rpl36_p2	rps11-rpl36	2	TACGTGCACTTTTACGCGAA	AAAATGTCGACTGATCCGCA	20	20	45.0	45.0	58.5	57.9	15.9	17.9	0.607910	79268	79423	301-305	1.000000	78.8
rps11-rpl36_p3	rps11-rpl36	3	TTACGTGCACTTTTACGCGA	AAAATGTCGACTGATCCGCA	20	20	45.0	45.0	58.5	57.9	15.9	17.9	0.607910	79268	79423	302-306	1.000000	78.8
rps11-rpl36_p4	rps11-rpl36	4	TTTACGTGCACTTTTACGCG	GGGCTTCTGTTCGTCCAATT	20	20	45.0	50.0	57.7	58.5	16.2	0.0	0.747552	79268	79423	328-332	1.000000	77.4
rps11-rpl36_p5	rps11-rpl36	5	TTTACGTGCACTTTTACGCG	TAAGGGCTTCTGTTCGTCCA	20	20	45.0	50.0	57.7	58.7	16.2	0.0	0.938215	79268	79423	331-335	1.000000	75.9
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	GGCAGAATAGCAGAGCAAGT	ATTCCGAATGGCTTTGGTGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.112174	106960	109071	2343-2503	1.000000	85.9
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	TGGGTCTTTTGTCGAAGTGG	ATTCCGAATGGCTTTGGTGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.114851	106960	109071	2453-2615	1.000000	85.8
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGCAGAATAGCAGAGCAAGT	TGGCTTTGGTGTTACAAGCT	20	20	50.0	45.0	58.0	57.9	0.0	6.7	0.101429	106960	109071	2495-2495	0.500000	59.2
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	TGGGTCTTTTGTCGAAGTGG	TGGCTTTGGTGTTACAAGCT	20	20	50.0	45.0	58.0	57.9	0.0	6.7	0.104105	106960	109071	2607-2607	0.500000	59.2
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GCTTCCCTACTCTTAACGGC	TGGCTTTGGTGTTACAAGCT	20	20	55.0	45.0	58.1	57.9	0.0	6.7	0.128621	106960	109071	2390-2390	0.500000	59.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTAAAAGCCTTTT	CTCGCACTGCTTCCTAAGAG	20	20	45.0	55.0	57.9	58.1	7.7	0.0	0.228997	112569	113355	912-913	1.000000	85.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTAAAAGCCTTTT	CTCTCGCACTGCTTCCTAAG	20	20	45.0	55.0	57.9	58.1	7.7	0.0	0.228997	112569	113355	914-915	1.000000	85.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTAAAAGCCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.9	57.9	7.7	0.0	0.273193	112569	113355	901-902	1.000000	85.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCAGCGTTAAAAGCCTTTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.9	57.8	7.7	0.0	0.333920	112569	113355	894-895	1.000000	84.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCGTTAAAAGCCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.9	57.8	7.7	0.0	0.345211	112569	113355	899-900	1.000000	84.4
ycf1_p1	ycf1	1	GGATCTTTTATGATCAATCCCTCT	ATGAAACTGGAACCAACCCA	24	20	37.5	45.0	56.0	57.2	9.8	0.0	6.736152	127606	127725	167-167	1.000000	47.7
ycf1_p2	ycf1	2	AGGATCTTTTATGATCAATCCCTCT	ATGAAACTGGAACCAACCCA	25	20	36.0	45.0	57.4	57.2	15.3	0.0	6.410821	127606	127725	168-168	1.000000	47.6
ycf1_p3	ycf1	3	AGGATCTTTTATGATCAATCCCTC	ATGAAACTGGAACCAACCCA	24	20	37.5	45.0	56.0	57.2	15.3	0.0	6.736152	127606	127725	168-168	1.000000	47.6
ycf1_p4	ycf1	4	AAGGATCTTTTATGATCAATCCCTCT	ATGAAACTGGAACCAACCCA	26	20	34.6	45.0	57.9	57.2	15.3	0.0	6.820620	127606	127725	169-169	1.000000	47.6
ycf1_p5	ycf1	5	AGGATCTTTTATGATCAATCCCTCTT	ATGAAACTGGAACCAACCCA	26	20	34.6	45.0	57.9	57.2	15.3	0.0	6.820620	127606	127725	168-168	1.000000	47.6