primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CACATTCGTCTCCACTCAGG TGAAACGATTCGGGATCACC 20 20 55.0 50.0 58.0 58.0 0.0 11.3 0.020354 4332 5095 1936-1936 0.500000 59.8 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 TTCTTGGGCCAGCAAATGAT TGAAACGATTCGGGATCACC 20 20 45.0 50.0 58.1 58.0 6.2 11.3 0.077165 4332 5095 2163-2163 0.500000 59.4 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 TCAGGATCAGTCGTGGTCTT TGAAACGATTCGGGATCACC 20 20 50.0 50.0 58.1 58.0 0.0 11.3 0.093811 4332 5095 1539-1539 0.500000 59.2 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 ATTCACTCGACCCAACCATG TGAAACGATTCGGGATCACC 20 20 50.0 50.0 57.9 58.0 0.0 11.3 0.132402 4332 5095 1705-1705 0.500000 58.9 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 CACTCGACCCAACCATGAAT TGAAACGATTCGGGATCACC 20 20 50.0 50.0 57.9 58.0 0.0 11.3 0.132402 4332 5095 1702-1702 0.500000 58.9 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.2 58.1 0.0 16.9 0.863824 6150 7482 1206-1398 1.000000 85.1 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 CGTTGCTTTCTACCACATCG CTCGGAGGTTCGAATCCTTC 20 20 50.0 55.0 57.2 57.8 0.0 16.9 0.999075 6150 7482 1210-1402 1.000000 84.0 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 59.1 58.1 0.0 16.9 1.134559 6150 7482 1209-1401 1.000000 82.9 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CGTTGCTTTCTACCACATCG ACTCGGAGGTTCGAATCCTT 20 20 50.0 50.0 57.2 58.4 0.0 16.9 1.229490 6150 7482 1211-1403 1.000000 82.2 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GCACGTTGCTTTCTACCACA CTCGGAGGTTCGAATCCTTC 20 20 50.0 55.0 59.1 57.8 0.0 16.9 1.269810 6150 7482 1213-1405 1.000000 81.8 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CTTCCGCCAATCCTTGATCA CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.124814 27465 28507 1123-1205 1.000000 90.3 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 CCGGGGCTTTGCAATATTTG CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.143503 27465 28507 1415-1497 1.000000 89.6 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 GAAAATACCGGATCCCCACC CCGGATTTGAACTGGGGAAA 20 20 55.0 50.0 58.0 58.1 9.5 0.0 0.105072 27465 28507 1742-1824 1.000000 88.9 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 CCCCACCTACACAAGCAAAT CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.158772 27465 28507 1729-1811 1.000000 88.6 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 AAATTAATCCCGCGGACACA CCGGATTTGAACTGGGGAAA 20 20 45.0 50.0 57.9 58.1 30.7 0.0 0.198955 27465 28507 1367-1367 0.500000 58.4 trnC-GCA-petN_p1 trnC-GCA-petN 1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.088172 28579 29460 945-962 1.000000 90.1 trnC-GCA-petN_p2 trnC-GCA-petN 2 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.273256 28579 29460 939-956 1.000000 88.6 trnC-GCA-petN_p3 trnC-GCA-petN 3 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.4 58.0 14.7 0.0 0.361535 28579 29460 963-980 1.000000 87.8 trnC-GCA-petN_p4 trnC-GCA-petN 4 GGGGGACTGCAAATCCTTTT CAGCCCAAGCAAGACTTACT 20 20 50.0 50.0 58.4 57.8 14.7 0.0 0.546619 28579 29460 957-974 1.000000 86.4 trnC-GCA-petN_p5 trnC-GCA-petN 5 TTTCCCCAGTTCAAATCCGG CATTAAAGCAGCCCAAGCAA 20 20 50.0 45.0 58.1 57.3 0.0 0.0 0.829524 28579 29460 947-964 1.000000 84.1 petN-psbM_p1 petN-psbM 1 TCTTGCTTGGGCTGCTTTAA GTCAAGAGTTGTGGAGTGGG 20 20 45.0 55.0 58.0 58.1 0.0 0.0 0.121809 29551 30773 1882-1961 1.000000 91.0 petN-psbM_p2 petN-psbM 2 TCTTGCTTGGGCTGCTTTAA TCAAGAGTTGTGGAGTGGGA 20 20 45.0 50.0 58.0 58.2 0.0 0.0 0.204802 29551 30773 1881-1960 1.000000 90.4 petN-psbM_p3 petN-psbM 3 TTGACTCTGCGGCATTGATT GTCAAGAGTTGTGGAGTGGG 20 20 45.0 55.0 58.2 58.1 1.7 0.0 0.290674 29551 30773 1984-2057 1.000000 89.4 petN-psbM_p4 petN-psbM 4 TTTGACTCTGCGGCATTGAT GTCAAGAGTTGTGGAGTGGG 20 20 45.0 55.0 58.2 58.1 1.7 0.0 0.290674 29551 30773 1985-2058 1.000000 89.4 petN-psbM_p5 petN-psbM 5 ATCCCTTTTTGACTCTGCGG GTCAAGAGTTGTGGAGTGGG 20 20 50.0 55.0 58.2 58.1 0.0 0.0 0.289380 29551 30773 2065-2065 0.500000 57.7 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 TATTAGGTCGGGACTGACGG TGGCAATATGTCTACGCTGG 20 20 55.0 50.0 58.3 57.8 5.3 0.0 0.554999 31553 31990 533-560 1.000000 87.6 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 TATTAGGTCGGGACTGACGG GGCAATATGTCTACGCTGGT 20 20 55.0 50.0 58.3 57.8 5.3 0.0 0.556550 31553 31990 532-559 1.000000 87.5 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 TATTAGGTCGGGACTGACGG GGGGACGGACTGTAAATTCG 20 20 55.0 55.0 58.3 58.4 5.3 0.0 0.667553 31553 31990 554-581 1.000000 86.4 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.716274 31553 31990 481-508 1.000000 86.3 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 TATTAGGTCGGGACTGACGG GGGACGGACTGTAAATTCGT 20 20 55.0 50.0 58.3 57.6 5.3 0.0 0.691230 31553 31990 553-580 1.000000 86.2 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 20 20 55.0 50.0 57.8 58.0 0.0 0.0 0.165150 32207 32936 871-893 1.000000 90.7 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 TTTCGTAGTACCCTACCCCC TACCGCTGAGTTAAAAGGGC 20 20 55.0 50.0 57.8 57.9 0.0 0.0 0.262716 32207 32936 836-858 1.000000 89.9 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 20 20 55.0 50.0 58.3 58.0 0.0 0.0 0.269359 32207 32936 833-855 1.000000 89.8 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.287509 32207 32936 812-834 1.000000 89.7 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 20 20 55.0 55.0 57.8 58.2 0.0 0.0 0.310083 32207 32936 868-890 1.000000 89.5 psbZ-trnG-GCC_p1 psbZ-trnG-GCC 1 CCGGTACATCATTATGGATTGGA CGTCTTCTCCTTGGCAAAGA 23 20 43.5 50.0 58.1 57.8 0.0 1.1 3.264404 37666 38110 497-557 1.000000 64.6 psbZ-trnG-GCC_p2 psbZ-trnG-GCC 2 TCCGGTACATCATTATGGATTGG CGTCTTCTCCTTGGCAAAGA 23 20 43.5 50.0 58.1 57.8 0.0 1.1 3.264404 37666 38110 498-558 1.000000 64.6 psbZ-trnG-GCC_p3 psbZ-trnG-GCC 3 CCGGTACATCATTATGGATTGG CGTCTTCTCCTTGGCAAAGA 22 20 45.5 50.0 56.7 57.8 0.0 1.1 3.467182 37666 38110 497-557 1.000000 62.9 psbZ-trnG-GCC_p4 psbZ-trnG-GCC 4 CCGGTACATCATTATGGATTGGA TACCATTCGACCATATCCGC 23 20 43.5 50.0 58.1 57.3 0.0 0.0 3.830715 37666 38110 470-530 1.000000 60.4 psbZ-trnG-GCC_p5 psbZ-trnG-GCC 5 TCCGGTACATCATTATGGATTGG TACCATTCGACCATATCCGC 23 20 43.5 50.0 58.1 57.3 0.0 0.0 3.830715 37666 38110 471-531 1.000000 60.4 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 48370 49043 724-944 1.000000 88.9 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 48370 49043 732-952 1.000000 88.4 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 48370 49043 756-976 1.000000 87.9 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 48370 49043 764-984 1.000000 87.3 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG ATCCAATCAAGTCCGTAGCG 20 20 55.0 50.0 57.9 57.8 0.0 0.0 0.357808 48370 49043 747-967 1.000000 87.0 trnL-UAA-trnF-GAA_p1 trnL-UAA-trnF-GAA 1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.5 58.1 0.0 20.1 0.526305 49639 50042 477-494 1.000000 85.2 trnL-UAA-trnF-GAA_p2 trnL-UAA-trnF-GAA 2 AATCGTGAGGGTTCAAGTCC CACATGCCAGGAACCAGATT 20 20 50.0 50.0 57.5 58.2 0.0 0.0 0.631042 49639 50042 495-512 1.000000 84.2 trnL-UAA-trnF-GAA_p3 trnL-UAA-trnF-GAA 3 AATCGTGAGGGTTCAAGTCC ATTAGCCACATGCCAGGAAC 20 20 50.0 50.0 57.5 58.2 0.0 3.8 0.706651 49639 50042 501-518 1.000000 83.5 trnL-UAA-trnF-GAA_p4 trnL-UAA-trnF-GAA 4 AATCGTGAGGGTTCAAGTCC TACCAACTGAGCTATCCCGA 20 20 50.0 50.0 57.5 57.6 0.0 0.0 0.921818 49639 50042 439-456 1.000000 82.5 trnL-UAA-trnF-GAA_p5 trnL-UAA-trnF-GAA 5 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 20 20 50.0 50.0 57.5 57.5 0.0 20.1 0.931776 49639 50042 474-491 1.000000 82.0 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 CCTCGTGTCACCAGTTCAAA CGTAAGGATTATATTGCCCCCA 20 22 50.0 45.5 58.1 57.7 0.0 0.0 2.347036 50116 50847 792-817 1.000000 71.4 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 AATCCTCGTGTCACCAGTTC CGTAAGGATTATATTGCCCCCA 20 22 50.0 45.5 57.5 57.7 0.0 0.0 2.752507 50116 50847 795-820 1.000000 68.1 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 TCGGGATAGCTCAGTTGGTA CGTAAGGATTATATTGCCCCCA 20 22 50.0 45.5 57.6 57.7 0.0 0.0 2.742549 50116 50847 830-855 1.000000 67.9 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 TGGTAGAGCAGAGGACTGAA CGTAAGGATTATATTGCCCCCA 20 22 50.0 45.5 57.4 57.7 0.0 0.0 2.900052 50116 50847 815-840 1.000000 66.8 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 TTGGTAGAGCAGAGGACTGA CGTAAGGATTATATTGCCCCCA 20 22 50.0 45.5 57.4 57.7 0.0 0.0 2.900052 50116 50847 816-841 1.000000 66.8 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 22 20 36.4 50.0 56.1 57.8 0.0 0.0 4.083948 52550 53015 522-1412 1.000000 56.4 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 22 20 36.4 55.0 56.1 57.5 0.0 0.0 4.427720 52550 53015 521-1411 1.000000 53.7 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 22 20 36.4 55.0 56.1 58.7 0.0 0.0 4.570014 52550 53015 510-1400 1.000000 52.5 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 CATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 21 20 38.1 50.0 54.6 57.8 0.0 0.0 4.585537 52550 53015 521-1411 1.000000 52.4 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 TCATATTCGTGAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 23 20 34.8 50.0 56.6 57.8 0.0 0.0 4.587896 52550 53015 522-1412 1.000000 52.4 rbcL-accD_p1 rbcL-accD 1 AATGAGGGACGTGATCTTGC CCGAATGTTGCTGACACCTA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.123145 58121 58866 1058-1085 1.000000 89.5 rbcL-accD_p2 rbcL-accD 2 ACTACAGTTCGGTGGAGGAA TCTTCACCCCCATCGGTATT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.117573 58121 58866 1067-1094 1.000000 89.4 rbcL-accD_p3 rbcL-accD 3 ACTACAGTTCGGTGGAGGAA CCGAATGTTGCTGACACCTA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.098676 58121 58866 1155-1182 1.000000 89.0 rbcL-accD_p4 rbcL-accD 4 TTGAATGCTACTGCGGGTAC CCGAATGTTGCTGACACCTA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.111848 58121 58866 1634-1661 1.000000 86.7 rbcL-accD_p5 rbcL-accD 5 AGAGAGATCACCTTGGGCTT CCGAATGTTGCTGACACCTA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.129321 58121 58866 1364-1364 0.500000 59.0 psbE-petL_p1 psbE-petL 1 CAACCCGCAATGAATAGGGA ACCGAAATAACTAGTTATAGTAGGCA 20 26 50.0 34.6 57.9 57.5 6.4 24.9 6.520644 65510 66697 1315-1319 1.000000 50.8 psbE-petL_p2 psbE-petL 2 ACCAACCCGCAATGAATAGG ACCGAAATAACTAGTTATAGTAGGCA 20 26 50.0 34.6 58.2 57.5 0.0 24.9 6.706602 65510 66697 1317-1321 1.000000 50.8 psbE-petL_p3 psbE-petL 3 CCGCAATGAATAGGGAAGGT ACCGAAATAACTAGTTATAGTAGGCA 20 26 50.0 34.6 57.7 57.5 0.0 24.9 6.817720 65510 66697 1311-1315 1.000000 50.8 psbE-petL_p4 psbE-petL 4 ACGAATAACCAACCCGCAAT ACCGAAATAACTAGTTATAGTAGGCA 20 26 45.0 34.6 58.2 57.5 0.0 24.9 6.640330 65510 66697 1324-1328 1.000000 50.7 psbE-petL_p5 psbE-petL 5 CGAATAACCAACCCGCAATG ACCGAAATAACTAGTTATAGTAGGCA 20 26 50.0 34.6 57.8 57.5 0.0 24.9 6.656660 65510 66697 1323-1327 1.000000 50.7 trnN-GUU-ndhF_p1 trnN-GUU-ndhF 1 TCAAAGCGAGCCCTTTCATT GAGGTCCTCGGAGAACTCTT 20 20 45.0 55.0 58.1 58.2 0.0 6.6 0.248249 110414 112927 2947-2947 0.500000 58.0 trnN-GUU-ndhF_p2 trnN-GUU-ndhF 2 GGAACGGGTTACCTACTCCT GAGGTCCTCGGAGAACTCTT 20 20 55.0 55.0 58.1 58.2 0.0 6.6 0.299984 110414 112927 2972-2972 0.500000 57.6 trnN-GUU-ndhF_p3 trnN-GUU-ndhF 3 AGGATTCGAACCTACGACCA GAGGTCCTCGGAGAACTCTT 20 20 50.0 55.0 58.2 58.2 15.0 6.6 0.314580 110414 112927 2887-2887 0.500000 57.5 trnN-GUU-ndhF_p4 trnN-GUU-ndhF 4 AAAGCGAGCCCTTTCATTCT GAGGTCCTCGGAGAACTCTT 20 20 45.0 55.0 57.8 58.2 0.0 6.6 0.364525 110414 112927 2945-2945 0.500000 57.1 trnN-GUU-ndhF_p5 trnN-GUU-ndhF 5 TCAAAGCGAGCCCTTTCATT AGGTCCTCGGAGAACTCTTT 20 20 45.0 50.0 58.1 57.7 0.0 6.6 0.402724 110414 112927 2946-2946 0.500000 56.8 ndhF-rpl32_p1 ndhF-rpl32 1 CCCGAAATTCAAAACTGCCC TTTGCTGCCCAGTATGCTTT 20 20 50.0 45.0 57.9 58.4 0.0 0.0 0.447444 115163 115946 1489-1489 0.500000 56.4 ndhF-rpl32_p2 ndhF-rpl32 2 CCCGAAATTCAAAACTGCCC TTTTGCTGCCCAGTATGCTT 20 20 50.0 45.0 57.9 58.4 0.0 0.0 0.447444 115163 115946 1490-1490 0.500000 56.4 ndhF-rpl32_p3 ndhF-rpl32 3 CCCGAAATTCAAAACTGCCC TTTTTGCTGCCCAGTATGCT 20 20 50.0 45.0 57.9 58.4 0.0 0.0 0.447444 115163 115946 1491-1491 0.500000 56.4 ndhF-rpl32_p4 ndhF-rpl32 4 GACGGGAAAAGGAGAAGACC TTTGCTGCCCAGTATGCTTT 20 20 55.0 45.0 57.9 58.4 0.0 0.0 0.464815 115163 115946 962-962 0.500000 56.3 ndhF-rpl32_p5 ndhF-rpl32 5 GACGGGAAAAGGAGAAGACC TTTTGCTGCCCAGTATGCTT 20 20 55.0 45.0 57.9 58.4 0.0 0.0 0.464815 115163 115946 963-963 0.500000 56.3 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 ATGACGAGAATACGCGGAAG TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 57.9 57.9 12.5 0.0 0.245393 116109 116868 1245-1245 0.500000 58.0 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 CATGACGAGAATACGCGGAA TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 58.2 57.9 12.5 0.0 0.289156 116109 116868 1246-1246 0.500000 57.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AACCTTTCCTCGAACTTCGG TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 57.8 57.9 11.2 0.0 0.289596 116109 116868 1079-1079 0.500000 57.7 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 ATGACGAGAATACGCGGAAG AGCAGCGTGTCTACCAATTT 20 20 50.0 45.0 57.9 57.8 12.5 0.0 0.306120 116109 116868 1238-1238 0.500000 57.6 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 ATGACGAGAATACGCGGAAG CTAAGAGCAGCGTGTCTACC 20 20 50.0 55.0 57.9 57.8 12.5 0.0 0.317411 116109 116868 1243-1243 0.500000 57.5