primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.307743	4258	4895	851-893	0.571429	66.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGATACCCCGGAGACAGAAG	GCAAAGACAACGAATTCCGG	20	20	55.0	50.0	57.9	58.0	0.7	0.0	0.110982	4258	4895	1953-1954	0.285714	50.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.204673	4258	4895	1264-1265	0.285714	50.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.204673	4258	4895	1263-1264	0.285714	50.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGATACCCCGGAGACAGAAG	TCGATCCCGAAGAGAAGGAA	20	20	55.0	50.0	57.9	57.9	0.7	0.0	0.214052	4258	4895	1541-1542	0.285714	50.0
rps16_p1	rps16	1	TATTTGTTCGAGACGCCGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.9	58.1	0.0	16.9	0.144938	4896	6055	1890-1958	0.571429	68.2
rps16_p2	rps16	2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4896	6055	2255-2336	0.571429	67.7
rps16_p3	rps16	3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4896	6055	2254-2335	0.571429	67.7
rps16_p4	rps16	4	TATTTGTTCGAGACGCCGTT	AATCCTTCCGTCCCAGATCA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.189262	4896	6055	1882-1888	0.285714	50.0
rps16_p5	rps16	5	AAGCCGAGTACTCTACCGTT	AATCCTTCCGTCCCAGATCA	20	20	50.0	50.0	58.2	58.1	20.3	0.0	0.288845	4896	6055	2246-2249	0.285714	49.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCGGAATTCGTTGTCTTTGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.112354	6056	6454	1006-1060	1.000000	88.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CCGGAATTCGTTGTCTTTGC	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.092347	6056	6454	1593-1652	1.000000	87.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCGTTGTCTTTGCTTTTCGC	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.155727	6056	6454	1586-1589	0.285714	50.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGTTGTCTTTGCTTTTCGCT	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.161417	6056	6454	1585-1588	0.285714	50.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCGTTGTCTTTGCTTTTCGC	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.9	58.1	0.0	16.9	0.175734	6056	6454	1003-1007	0.285714	50.2
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	TCATGTCCTTCAAGTCGCAC	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.260327	6527	6885	1059-1087	1.000000	87.8
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	TGCTCGAGCTGCATCATATG	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.2	57.9	35.3	0.0	0.264088	6527	6885	1208-1236	1.000000	87.2
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	AAGTCGCACGTTGCTTTCTA	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	58.1	57.9	8.1	0.0	0.195418	6527	6885	1053-1076	0.857143	80.7
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	AAGTCGCACGTTGCTTTCTA	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.1	58.1	8.1	0.7	0.206303	6527	6885	1596-1636	0.428571	60.6
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	AAGTCGCACGTTGCTTTCTA	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.1	58.1	8.1	0.7	0.206303	6527	6885	1597-1637	0.428571	60.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034421	7880	8501	1376-1443	1.000000	86.3
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035809	7880	8501	1375-1442	1.000000	86.3
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	GATCAGGGGAATCGGAGAGA	CATCGTTAGCTTGGAAGGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.032344	7880	8501	1445-1498	0.571429	67.5
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATCAGGGGAATCGGAGAGAG	CATCGTTAGCTTGGAAGGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.038081	7880	8501	1444-1497	0.571429	67.5
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	GATCAGGGGAATCGGAGAGA	CGTTAGCTTGGAAGGCTAGG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.061180	7880	8501	1442-1495	0.571429	67.3
atpH-atpI_p1	atpH-atpI	1	AGCCAATCCAGCAGCAATAA	GGCTGCGGCTTATATAGGTG	20	20	45.0	55.0	57.9	58.3	0.0	18.8	0.421592	12935	13670	821-833	1.000000	87.1
atpH-atpI_p2	atpH-atpI	2	GCCAATCCAGCAGCAATAAC	GGCTGCGGCTTATATAGGTG	20	20	50.0	55.0	57.8	58.3	0.0	18.8	0.487534	12935	13670	820-832	1.000000	86.6
atpH-atpI_p3	atpH-atpI	3	TCCAGCAGCAATAACGGAAG	GGCTGCGGCTTATATAGGTG	20	20	50.0	55.0	58.3	58.3	0.0	18.8	0.535308	12935	13670	815-827	1.000000	86.2
atpH-atpI_p4	atpH-atpI	4	AATCCAGCAGCAATAACGGA	GGCTGCGGCTTATATAGGTG	20	20	45.0	55.0	57.6	58.3	0.0	18.8	0.691515	12935	13670	817-829	1.000000	85.0
atpH-atpI_p5	atpH-atpI	5	ATCCAGCAGCAATAACGGAA	GGCTGCGGCTTATATAGGTG	20	20	45.0	55.0	57.6	58.3	0.0	18.8	0.691515	12935	13670	816-828	1.000000	85.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.3	57.9	11.5	0.0	0.343293	29112	30056	1050-1087	1.000000	84.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.340822	29112	30056	1102-1139	1.000000	84.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	57.6	57.9	11.5	0.0	0.464499	29112	30056	1049-1086	1.000000	83.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAATGAACAGCGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.5	57.9	11.5	0.0	0.563386	29112	30056	1052-1089	1.000000	83.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	58.5	57.9	11.5	0.0	0.563386	29112	30056	1051-1088	1.000000	83.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	30627	31493	971-992	1.000000	83.5
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	30627	31493	950-971	1.000000	83.1
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	30627	31493	947-968	1.000000	83.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30627	31493	949-970	1.000000	82.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	30627	31493	968-989	1.000000	82.3
rps14-psaB_p1	rps14-psaB	1	CGGTGGGGATTGCAATTTTC	ACTTACGCGGCTTTCTTGAT	20	20	50.0	45.0	58.0	57.9	14.3	12.5	0.103514	36618	36749	326-348	1.000000	88.1
rps14-psaB_p2	rps14-psaB	2	GTTCCAATTTCTGGCGCTTC	CCAGTGGCTCTTTCCATTGT	20	20	50.0	50.0	58.0	58.1	7.6	0.0	0.102247	36618	36749	294-316	1.000000	88.0
rps14-psaB_p3	rps14-psaB	3	CGGTGGGGATTGCAATTTTC	ATTCACTTACGCGGCTTTCT	20	20	50.0	45.0	58.0	57.9	14.3	12.5	0.103514	36618	36749	330-352	1.000000	88.0
rps14-psaB_p4	rps14-psaB	4	CGGTGGGGATTGCAATTTTC	CCAGTGGCTCTTTCCATTGT	20	20	50.0	50.0	58.0	58.1	14.3	0.0	0.093611	36618	36749	395-417	1.000000	86.9
rps14-psaB_p5	rps14-psaB	5	CGGTGGGGATTGCAATTTTC	CAATAGGGCCGGGAGATTTC	20	20	50.0	55.0	58.0	58.1	14.3	2.2	0.111192	36618	36749	952-974	1.000000	82.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAGATAGGAATAACGCT	CCGAGAAAGTCTACGGTTCG	21	20	42.9	55.0	57.2	58.1	0.0	0.0	1.945776	49721	50859	1191-1322	1.000000	73.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAGATAGGAATAACGCTT	CCGAGAAAGTCTACGGTTCG	22	20	40.9	55.0	57.8	58.1	0.0	0.0	2.258429	49721	50859	1191-1322	1.000000	70.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATGCCAAGATAGGAATAACGCT	CCGAGAAAGTCTACGGTTCG	22	20	40.9	55.0	57.6	58.1	0.0	0.0	2.465451	49721	50859	1192-1323	1.000000	68.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAGATAGGAATAACGC	CCGAGAAAGTCTACGGTTCG	20	20	45.0	55.0	55.6	58.1	0.0	0.0	2.491702	49721	50859	1191-1322	1.000000	68.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAGATAGGAATAACGCT	GTTCGAGTCCGTATAGCCCT	21	20	42.9	55.0	57.2	58.7	0.0	0.0	2.521593	49721	50859	1176-1307	1.000000	68.5
psaJ-rpl33_p1	psaJ-rpl33	1	TTGGGTCTTTAGCGGGTCTA	GGTCAACCTATTCACCCGTC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.078570	66328	66896	1187-1220	1.000000	87.4
psaJ-rpl33_p2	psaJ-rpl33	2	CGAACTGAACTAAGAGCGCT	GGTCAACCTATTCACCCGTC	20	20	50.0	55.0	58.0	58.0	11.3	0.0	0.035975	66328	66896	1833-1872	1.000000	86.9
psaJ-rpl33_p3	psaJ-rpl33	3	ACCCGTGACATTTTGTACCC	GGTCAACCTATTCACCCGTC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.119702	66328	66896	1649-1677	1.000000	86.7
psaJ-rpl33_p4	psaJ-rpl33	4	CGAACTGAACTAAGAGCGCT	TAATCGATTCGATCCCCCGA	20	20	50.0	50.0	58.0	58.1	11.3	21.0	0.102250	66328	66896	1757-1796	1.000000	86.6
psaJ-rpl33_p5	psaJ-rpl33	5	CGAACTGAACTAAGAGCGCT	TGAGCTTGAGTTCCGATTGG	20	20	50.0	50.0	58.0	57.9	11.3	0.0	0.115169	66328	66896	2011-2050	1.000000	85.8
clpP_p1	clpP	1	GGTTGGCATGTCGTGTCTAT	CGAACAATAGGCCGATCCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.076071	69251	71504	2731-2830	1.000000	87.9
clpP_p2	clpP	2	AGTCGCACATACACCCTAGT	CGAACAATAGGCCGATCCAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.203244	69251	71504	2844-2943	1.000000	86.8
clpP_p3	clpP	3	TAATGGTTGGCATGTCGTGT	CGAACAATAGGCCGATCCAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.244559	69251	71504	2735-2834	1.000000	86.5
clpP_p4	clpP	4	GGTTGGCATGTCGTGTCTAT	TTCGAACAATAGGCCGATCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.258475	69251	71504	2733-2832	1.000000	86.4
clpP_p5	clpP	5	GGTTGGCATGTCGTGTCTAT	CCAATACGCAATGGGAGGTT	20	20	50.0	50.0	58.0	58.2	0.0	3.8	0.261468	69251	71504	2798-2897	1.000000	86.4
rpl36-infA_p1	rpl36-infA	1	TCATAACCTCTGCTCGTTGC	AGAATCCGGCGCAGTTTTAT	20	20	50.0	45.0	58.0	58.0	0.0	10.8	0.048532	79820	79941	795-822	1.000000	86.2
rpl36-infA_p2	rpl36-infA	2	AAACCACTCCGTGCATTCTT	GGCGGAATGGGGATTGTAAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.023367	79820	79941	894-934	1.000000	85.9
rpl36-infA_p3	rpl36-infA	3	TCATAACCTCTGCTCGTTGC	GGCGGAATGGGGATTGTAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014769	79820	79941	1124-1164	1.000000	85.6
rpl36-infA_p4	rpl36-infA	4	GAAACCACTCGACCTCGTAC	GGCGGAATGGGGATTGTAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.030137	79820	79941	996-1036	1.000000	85.6
rpl36-infA_p5	rpl36-infA	5	CAGAAGCCCAAGAAACCACT	GGCGGAATGGGGATTGTAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.017741	79820	79941	1008-1025	0.714286	71.3
rpl16-rps3_p1	rpl16-rps3	1	CTGAACTCTGCAATGGAGCT	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148287	83009	83188	981-1031	1.000000	84.8
rpl16-rps3_p2	rpl16-rps3	2	TCGTTCCCATAGCTTCTCCA	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.176744	83009	83188	1014-1064	1.000000	84.5
rpl16-rps3_p3	rpl16-rps3	3	AACTCTGCAATGGAGCTTCC	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.205805	83009	83188	978-1028	1.000000	84.4
rpl16-rps3_p4	rpl16-rps3	4	GTTTAGGCCTGAACTCTGCA	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	57.8	58.0	28.3	0.0	0.222389	83009	83188	989-1039	1.000000	84.2
rpl16-rps3_p5	rpl16-rps3	5	TGTTTAGGCCTGAACTCTGC	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	57.8	58.0	33.0	0.0	0.222389	83009	83188	990-1040	1.000000	84.2
ndhF-rpl32_p1	ndhF-rpl32	1	GGAGCGGAAAAAGGGGTATT	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.177530	113108	113828	491-911	1.000000	89.8
ndhF-rpl32_p2	ndhF-rpl32	2	ACATAGTAACTGGGAGCGGA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	57.8	58.0	0.0	9.5	0.192774	113108	113828	503-923	1.000000	89.5
ndhF-rpl32_p3	ndhF-rpl32	3	CATTCCCACGAGTTCCCAAA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.1	58.0	0.0	9.5	0.133920	113108	113828	910-1330	1.000000	88.5
ndhF-rpl32_p4	ndhF-rpl32	4	AACACATTCCCACGAGTTCC	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.1	58.0	0.0	9.5	0.145997	113108	113828	914-1334	1.000000	88.3
ndhF-rpl32_p5	ndhF-rpl32	5	TGGCCGAAAAACACATAGCT	TCCACCGGACAGTCAAAAAG	20	20	45.0	50.0	58.1	58.0	0.0	9.5	0.133920	113108	113828	950-1031	0.714286	73.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CTTTTTGACTGTCCGGTGGA	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.107041	114003	114655	1191-1301	1.000000	89.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.127156	114003	114655	1044-1154	0.571429	70.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGTTGAAATGAGCTGGCCAA	TGGCGAAACCTTGGGTAAAA	20	20	45.0	45.0	58.0	57.9	7.0	0.0	0.069186	114003	114655	1889-1889	0.285714	51.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGTTGAAATGAGCTGGCCAA	GTCCCGAAGAAATCCAACGA	20	20	45.0	50.0	58.0	57.9	7.0	0.0	0.089301	114003	114655	1742-1742	0.285714	51.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGTTGAAATGAGCTGGCCAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	7.0	0.0	0.124629	114003	114655	1416-1417	0.285714	50.7
ycf1_p1	ycf1	1	GGAATTTCTTGGGCCCCTTT	TCAAATTGAACAAGCCGCAA	20	20	50.0	40.0	58.3	57.1	22.3	0.0	1.222415	125778	125897	930-933	1.000000	76.9
ycf1_p2	ycf1	2	GGAATTTCTTGGGCCCCTTT	TGAACAAGCCGCAAAAGAAA	20	20	50.0	40.0	58.3	57.1	22.3	0.0	1.292864	125778	125897	924-927	1.000000	76.4
ycf1_p3	ycf1	3	GGAATTTCTTGGGCCCCTTT	TTGAACAAGCCGCAAAAGAA	20	20	50.0	40.0	58.3	57.1	22.3	0.0	1.292864	125778	125897	925-928	1.000000	76.4
ycf1_p4	ycf1	4	TATGGAATTTCTTGGGCCCC	TCAAATTGAACAAGCCGCAA	20	20	50.0	40.0	57.5	57.1	22.3	0.0	1.349059	125778	125897	933-933	0.285714	40.7
ycf1_p5	ycf1	5	TATGGAATTTCTTGGGCCCC	TGAACAAGCCGCAAAAGAAA	20	20	50.0	40.0	57.5	57.1	22.3	0.0	1.419508	125778	125897	927-927	0.285714	40.2