primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCGCG	20	20	55.0	50.0	57.6	58.6	0.0	0.0	1.077619	8010	8676	736-835	1.000000	79.5
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GATATGCTCTGGGACGGAAG	GGCTTCAGGTAATTTCGCGA	20	20	55.0	50.0	57.6	58.6	0.0	3.7	1.081545	8010	8676	735-834	1.000000	79.4
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCG	20	20	50.0	50.0	58.7	58.6	0.0	0.0	1.309868	8010	8676	694-793	1.000000	77.8
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTCGCGA	20	20	50.0	50.0	58.7	58.6	0.0	3.7	1.313794	8010	8676	693-792	1.000000	77.8
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCGCG	20	20	50.0	50.0	57.2	58.6	0.0	0.0	1.477702	8010	8676	740-839	1.000000	76.2
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCAGAA	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.791932	11975	12322	462-556	1.000000	72.7
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.793395	11975	12322	466-560	1.000000	72.7
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	21	20	47.6	50.0	57.3	58.4	0.0	3.1	2.071968	11975	12322	452-546	1.000000	70.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCAGAAG	21	20	47.6	50.0	57.3	57.6	0.0	0.0	2.086062	11975	12322	461-555	1.000000	70.4
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	21	20	47.6	50.0	57.3	57.5	0.0	0.0	2.165486	11975	12322	468-562	1.000000	69.7
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	AAGGACGATCAAAATGCGGA	CTTGAGGTCACGGGTTCAAA	20	20	45.0	50.0	57.9	58.0	0.0	4.2	0.146156	40702	40891	310-334	1.000000	83.3
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	CAAGGACGATCAAAATGCGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.216680	40702	40891	311-335	1.000000	82.7
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	AAGGACGATCAAAATGCGGA	TGAGGTCACGGGTTCAAATC	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.286862	40702	40891	308-332	1.000000	82.2
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	AAGGACGATCAAAATGCGGA	GTAGCTCGCAAGGCTCATAA	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.333018	40702	40891	331-355	1.000000	81.5
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	AAGGACGATCAAAATGCGGA	TAGCTCGCAAGGCTCATAAC	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.333018	40702	40891	330-354	1.000000	81.5
rpl33-rps18_p1	rpl33-rps18	1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	74014	74260	362-383	1.000000	86.9
rpl33-rps18_p2	rpl33-rps18	2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	74014	74260	363-384	1.000000	86.9
rpl33-rps18_p3	rpl33-rps18	3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.6	57.7	0.0	0.0	0.617852	74014	74260	364-385	1.000000	85.0
rpl33-rps18_p4	rpl33-rps18	4	TCTGTCCCTGTTGTTGCAAA	ATTCGATCCCCCGATTGGAT	20	20	45.0	50.0	57.9	58.6	0.0	0.0	0.774074	74014	74260	359-380	1.000000	83.9
rpl33-rps18_p5	rpl33-rps18	5	TTCTGTCCCTGTTGTTGCAA	ATTCGATCCCCCGATTGGAT	20	20	45.0	50.0	57.9	58.6	0.0	0.0	0.774074	74014	74260	360-381	1.000000	83.9
ycf1_p1	ycf1	1	TGAACTGCCGTGATTGCTTA	CGAAACGGCTGCCATTATTG	20	20	45.0	50.0	57.8	58.2	0.0	20.3	0.351067	122862	122981	684-684	0.500000	57.2
ycf1_p2	ycf1	2	TGAACTGCCGTGATTGCTTA	GAAACGGCTGCCATTATTGG	20	20	45.0	50.0	57.8	57.8	0.0	20.3	0.385720	122862	122981	683-683	0.500000	56.9
ycf1_p3	ycf1	3	AATTGAACTGCCGTGATTGC	CGAAACGGCTGCCATTATTG	20	20	45.0	50.0	57.6	58.2	0.0	20.3	0.526176	122862	122981	687-687	0.500000	55.8
ycf1_p4	ycf1	4	AATTGAACTGCCGTGATTGC	GAAACGGCTGCCATTATTGG	20	20	45.0	50.0	57.6	57.8	0.0	20.3	0.560829	122862	122981	686-686	0.500000	55.5
ycf1_p5	ycf1	5	TGAACTGCCGTGATTGCTTA	AACGGCTGCCATTATTGGTA	20	20	45.0	45.0	57.8	57.6	0.0	20.3	0.605938	122862	122981	681-681	0.500000	55.2
ndhH_p1	ndhH	1	GAAAACCCCGGATCGATTGA	TCAGGTCCAATACGCTGTTG	20	20	50.0	50.0	58.0	57.9	0.7	0.0	0.125987	124037	125218	1705-1705	0.500000	59.0
ndhH_p2	ndhH	2	AGAAAACCCCGGATCGATTG	TCAGGTCCAATACGCTGTTG	20	20	50.0	50.0	58.0	57.9	0.7	0.0	0.131693	124037	125218	1706-1706	0.500000	58.9
ndhH_p3	ndhH	3	GAAAACCCCGGATCGATTGA	CGAACAGAAAGGAGCATGGA	20	20	50.0	50.0	58.0	57.9	0.7	0.0	0.131822	124037	125218	2110-2110	0.500000	58.9
ndhH_p4	ndhH	4	AGAAAACCCCGGATCGATTG	CGAACAGAAAGGAGCATGGA	20	20	50.0	50.0	58.0	57.9	0.7	0.0	0.137528	124037	125218	2111-2111	0.500000	58.9
ndhH_p5	ndhH	5	GCACACACGCATTGTCTTAC	TCAGGTCCAATACGCTGTTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.142835	124037	125218	2071-2071	0.500000	58.9
ndhA_p1	ndhA	1	AGAGTTGAAGCACCAAAGGG	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.065013	125220	127699	2815-2815	0.500000	59.5
ndhA_p2	ndhA	2	AGAGTTGAAGCACCAAAGGG	TTGCAGCTCGTACTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.065013	125220	127699	2816-2816	0.500000	59.5
ndhA_p3	ndhA	3	AGTTGAAGCACCAAAGGGAG	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.065013	125220	127699	2813-2813	0.500000	59.5
ndhA_p4	ndhA	4	AGTTGAAGCACCAAAGGGAG	TTGCAGCTCGTACTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.065013	125220	127699	2814-2814	0.500000	59.5
ndhA_p5	ndhA	5	GTTGAAGCACCAAAGGGAGA	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.070639	125220	127699	2812-2812	0.500000	59.4
ndhI-ndhG_p1	ndhI-ndhG	1	AACAAACAGTACGAGCTGCA	GTGCTGCAGCTACAAAATGG	20	20	45.0	50.0	58.1	58.0	1.0	36.6	0.065198	128265	129175	1544-1544	0.500000	59.5
ndhI-ndhG_p2	ndhI-ndhG	2	ACAAACAGTACGAGCTGCAA	GTGCTGCAGCTACAAAATGG	20	20	45.0	50.0	58.1	58.0	1.0	36.6	0.065198	128265	129175	1543-1543	0.500000	59.5
ndhI-ndhG_p3	ndhI-ndhG	3	AACAAACAGTACGAGCTGCA	CAAGAACCAGTCCCAACGAA	20	20	45.0	50.0	58.1	58.0	1.0	0.0	0.090102	128265	129175	1490-1490	0.500000	59.3
ndhI-ndhG_p4	ndhI-ndhG	4	ACAAACAGTACGAGCTGCAA	CAAGAACCAGTCCCAACGAA	20	20	45.0	50.0	58.1	58.0	1.0	0.0	0.090102	128265	129175	1489-1489	0.500000	59.3
ndhI-ndhG_p5	ndhI-ndhG	5	AATCAAATTGCTTTGGGCCG	GTGCTGCAGCTACAAAATGG	20	20	45.0	50.0	57.9	58.0	0.0	36.6	0.110982	128265	129175	1170-1170	0.500000	59.1
ndhG_p1	ndhG	1	CCTGGTCGTATCAATAAGGTCA	TTTCGGCTCGTGATCAATCC	22	20	45.5	50.0	57.3	58.3	0.0	0.0	3.013846	129176	129706	915-915	0.500000	35.9
ndhG_p2	ndhG	2	CCTGGTCGTATCAATAAGGTCA	CTGGTCCAATAGCTGCTTCA	22	20	45.5	50.0	57.3	57.6	0.0	7.8	3.074104	129176	129706	1073-1073	0.500000	35.4
ndhG_p3	ndhG	3	CCTGGTCGTATCAATAAGGTCA	TGGTCCAATAGCTGCTTCAG	22	20	45.5	50.0	57.3	57.6	0.0	7.8	3.074104	129176	129706	1072-1072	0.500000	35.4
ndhG_p4	ndhG	4	CCTGGTCGTATCAATAAGGTCA	CGGCTCGTGATCAATCCATA	22	20	45.5	50.0	57.3	57.6	0.0	0.0	3.104702	129176	129706	912-912	0.500000	35.2
ndhG_p5	ndhG	5	CCTGGTCGTATCAATAAGGTCA	TGTTCGAGCATCATTGACCA	22	20	45.5	45.0	57.3	57.5	0.0	0.0	3.136817	129176	129706	852-852	0.500000	34.9
ndhG-ndhE_p1	ndhG-ndhE	1	CCATTTTGTAGCTGCAGCAC	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	58.0	58.1	38.2	0.0	0.139173	129707	129954	1296-1296	0.500000	58.9
ndhG-ndhE_p2	ndhG-ndhE	2	TTCGTTGGGACTGGTTCTTG	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.164077	129707	129954	1350-1350	0.500000	58.7
ndhG-ndhE_p3	ndhG-ndhE	3	TTTCTGCCTTTTCGTTGGGA	ACATCCGTCCCAAGGTATCA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.185921	129707	129954	1360-1360	0.500000	58.5
ndhG-ndhE_p4	ndhG-ndhE	4	CCATTTTGTAGCTGCAGCAC	GTTCTCGGAGCAGAAGCAAT	20	20	50.0	50.0	58.0	58.3	38.2	0.0	0.281582	129707	129954	1328-1328	0.500000	57.7
ndhG-ndhE_p5	ndhG-ndhE	5	CCATTTTGTAGCTGCAGCAC	CTCTGTTCTCGGAGCAGAAG	20	20	50.0	55.0	58.0	57.7	38.2	16.7	0.289774	129707	129954	1332-1332	0.500000	57.7
ndhD_p1	ndhD	1	AAACAACTCGAAGCATGGGT	AATGAGGCCTGGGGATCATA	20	20	45.0	50.0	58.0	57.9	0.0	12.0	0.157278	130907	132400	2157-2157	0.500000	58.7
ndhD_p2	ndhD	2	AAACAACTCGAAGCATGGGT	TAATGAGGCCTGGGGATCAT	20	20	45.0	50.0	58.0	57.9	0.0	12.0	0.157278	130907	132400	2158-2158	0.500000	58.7
ndhD_p3	ndhD	3	AAACAACTCGAAGCATGGGT	TGAGGCCTGGGGATCATATT	20	20	45.0	50.0	58.0	57.9	0.0	12.0	0.157278	130907	132400	2155-2155	0.500000	58.7
ndhD_p4	ndhD	4	GGCATGAAACAACTCGAAGC	AATGAGGCCTGGGGATCATA	20	20	50.0	50.0	58.0	57.9	0.0	12.0	0.169432	130907	132400	2163-2163	0.500000	58.6
ndhD_p5	ndhD	5	GGCATGAAACAACTCGAAGC	TGAGGCCTGGGGATCATATT	20	20	50.0	50.0	58.0	57.9	0.0	12.0	0.169432	130907	132400	2161-2161	0.500000	58.6
ndhD-ccsA_p1	ndhD-ccsA	1	CGGTTTTGTTGCGGAATTGA	GGGCTACGCAGCTCTTTTAT	20	20	45.0	50.0	57.9	58.0	0.0	7.7	0.171987	132401	132676	1130-1130	0.500000	58.6
ndhD-ccsA_p2	ndhD-ccsA	2	CAGTTGACAAGGTCGAAGCT	GGGCTACGCAGCTCTTTTAT	20	20	50.0	50.0	58.1	58.0	0.0	7.7	0.173993	132401	132676	849-849	0.500000	58.6
ndhD-ccsA_p3	ndhD-ccsA	3	TCAGTTGACAAGGTCGAAGC	GGGCTACGCAGCTCTTTTAT	20	20	50.0	50.0	58.1	58.0	0.0	7.7	0.179208	132401	132676	850-850	0.500000	58.6
ndhD-ccsA_p4	ndhD-ccsA	4	TTATCTCGACGAAATGGGCG	GGGCTACGCAGCTCTTTTAT	20	20	50.0	50.0	58.2	58.0	0.0	7.7	0.197255	132401	132676	1229-1229	0.500000	58.4
ndhD-ccsA_p5	ndhD-ccsA	5	GGGCCGCGAGAGTTATTTAT	GGGCTACGCAGCTCTTTTAT	20	20	50.0	50.0	57.8	58.0	8.2	7.7	0.210392	132401	132676	934-934	0.500000	58.3
ccsA_p1	ccsA	1	GGTGTTTGTGAGAACCCCTT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.075196	132677	133648	1300-1300	0.500000	59.4
ccsA_p2	ccsA	2	GGTGTTTGTGAGAACCCCTT	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.076566	132677	133648	1299-1299	0.500000	59.4
ccsA_p3	ccsA	3	CGGTTTTGTTGCGGAATTGA	GTGAAATCGGTAGACACGCT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.134744	132677	133648	1742-1742	0.500000	58.9
ccsA_p4	ccsA	4	CGGTTTTGTTGCGGAATTGA	TGAAATCGGTAGACACGCTG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.136114	132677	133648	1741-1741	0.500000	58.9
ccsA_p5	ccsA	5	CAGTTGACAAGGTCGAAGCT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136750	132677	133648	1461-1461	0.500000	58.9
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.008182	133830	134885	1185-1185	0.500000	59.9
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009553	133830	134885	1186-1186	0.500000	59.9
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	AGGGATATTGGGCAGCATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	133830	134885	1186-1186	0.500000	59.9
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	AGGGATATTGGGCAGCATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014571	133830	134885	1187-1187	0.500000	59.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TTGATACGACCGAAAACGCT	GGGATATTGGGCAGCATTGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.069189	133830	134885	1395-1395	0.500000	59.4
rpl32-ndhF_p1	rpl32-ndhF	1	TCAATGCTGCCCAATATCCC	TAGGGCGTGTGAACCAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.054584	135054	136068	1611-1611	0.500000	59.6
rpl32-ndhF_p2	rpl32-ndhF	2	CAATGCTGCCCAATATCCCT	TAGGGCGTGTGAACCAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.059602	135054	136068	1610-1610	0.500000	59.5
rpl32-ndhF_p3	rpl32-ndhF	3	TCAATGCTGCCCAATATCCC	AGCAACTGAACCGCCAAATA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.096065	135054	136068	1820-1820	0.500000	59.2
rpl32-ndhF_p4	rpl32-ndhF	4	TCAATGCTGCCCAATATCCC	ATTTAGCAACTGAACCGCCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.096065	135054	136068	1824-1824	0.500000	59.2
rpl32-ndhF_p5	rpl32-ndhF	5	TCAATGCTGCCCAATATCCC	TAGCAACTGAACCGCCAAAT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.096065	135054	136068	1821-1821	0.500000	59.2
ndhF_p1	ndhF	1	TTTTTCTTGTAGCCCGGCTT	TGTGGATGTGTCTTCTTCCG	20	20	45.0	50.0	58.0	57.5	19.4	0.0	0.465967	137327	137446	724-724	0.500000	56.3
ndhF_p2	ndhF	2	CACCCTTTGCCTGTTTTTGG	TGTGGATGTGTCTTCTTCCG	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.503481	137327	137446	319-319	0.500000	56.0
ndhF_p3	ndhF	3	CCACCCTTTGCCTGTTTTTG	TGTGGATGTGTCTTCTTCCG	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.503481	137327	137446	320-320	0.500000	56.0
ndhF_p4	ndhF	4	ATTTGGCGGTTCAGTTGCTA	TGTGGATGTGTCTTCTTCCG	20	20	45.0	50.0	58.1	57.5	0.0	0.0	0.551387	137327	137446	846-846	0.500000	55.6
ndhF_p5	ndhF	5	TGGCGGTTCAGTTGCTAAAT	TGTGGATGTGTCTTCTTCCG	20	20	45.0	50.0	58.1	57.5	0.0	0.0	0.551387	137327	137446	843-843	0.500000	55.6
ndhF-trnN-GUU_p1	ndhF-trnN-GUU	1	AATATGTAGGGGGTGGCAGA	CGGGAGAATTGATGACTGCA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.234742	138307	140222	2354-2354	0.500000	58.1
ndhF-trnN-GUU_p2	ndhF-trnN-GUU	2	ATATGTAGGGGGTGGCAGAA	CGGGAGAATTGATGACTGCA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.234742	138307	140222	2353-2353	0.500000	58.1
ndhF-trnN-GUU_p3	ndhF-trnN-GUU	3	TATGTAGGGGGTGGCAGAAT	CGGGAGAATTGATGACTGCA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.234742	138307	140222	2352-2352	0.500000	58.1
ndhF-trnN-GUU_p4	ndhF-trnN-GUU	4	ATATGTAGGGGGTGGCAGAA	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.241852	138307	140222	2444-2444	0.500000	58.1
ndhF-trnN-GUU_p5	ndhF-trnN-GUU	5	TATGTAGGGGGTGGCAGAAT	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.241852	138307	140222	2443-2443	0.500000	58.1