primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnM(cat)-trnR(ccg)_p1	trnM(cat)-trnR(ccg)	1	ATTATGAGTCCGGTGCCTTC	GGTTCGATTCCCTTCATGCT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.666186	20905	21248	406-424	1.000000	84.9
trnM(cat)-trnR(ccg)_p2	trnM(cat)-trnR(ccg)	2	GATTATGAGTCCGGTGCCTT	GGTTCGATTCCCTTCATGCT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.666186	20905	21248	407-425	1.000000	84.9
trnM(cat)-trnR(ccg)_p3	trnM(cat)-trnR(ccg)	3	TATGAGTCCGGTGCCTTCAA	GGTTCGATTCCCTTCATGCT	20	20	50.0	50.0	58.7	58.0	0.0	0.0	0.772267	20905	21248	404-422	1.000000	84.1
trnM(cat)-trnR(ccg)_p4	trnM(cat)-trnR(ccg)	4	TTATGAGTCCGGTGCCTTCA	GGTTCGATTCCCTTCATGCT	20	20	50.0	50.0	58.7	58.0	0.0	0.0	0.772267	20905	21248	405-423	1.000000	84.1
trnM(cat)-trnR(ccg)_p5	trnM(cat)-trnR(ccg)	5	ATTATGAGTCCGGTGCCTTC	GGGGTTCGATTCCCTTCATG	20	20	50.0	55.0	57.4	58.3	0.0	20.6	0.941726	20905	21248	408-426	1.000000	82.6
trnK(ttt)-ycf12_p1	trnK(ttt)-ycf12	1	TCGAACCCAGAACAAATCGG	AGCTGACCTAAAACTTGCCA	20	20	50.0	45.0	58.2	57.3	0.0	0.0	0.853613	26502	26837	422-429	1.000000	83.6
trnK(ttt)-ycf12_p2	trnK(ttt)-ycf12	2	GGACTCGAACCCAGAACAAA	AGCTGACCTAAAACTTGCCA	20	20	50.0	45.0	57.8	57.3	0.0	0.0	0.906791	26502	26837	426-433	1.000000	83.2
trnK(ttt)-ycf12_p3	trnK(ttt)-ycf12	3	CGAACCCAGAACAAATCGGT	AGCTGACCTAAAACTTGCCA	20	20	50.0	45.0	58.5	57.3	0.0	0.0	1.137539	26502	26837	421-428	1.000000	81.3
trnK(ttt)-ycf12_p4	trnK(ttt)-ycf12	4	TCGAACCCAGAACAAATCGG	ACCGGTTGCTACTAGCTGA	20	19	50.0	52.6	58.2	58.0	0.0	0.0	1.224990	26502	26837	435-442	1.000000	80.6
trnK(ttt)-ycf12_p5	trnK(ttt)-ycf12	5	TCGAACCCAGAACAAATCGG	CACCGGTTGCTACTAGCTG	20	19	50.0	57.9	58.2	57.9	0.0	3.0	1.247369	26502	26837	436-443	1.000000	80.4
trnS(tga)-petB_p1	trnS(tga)-petB	1	GATGGCAGAGTTGGTCGATT	TCGTTCTTCAAACCAATCGT	20	20	50.0	40.0	58.0	55.6	0.0	0.0	2.402314	32847	32934	202-204	1.000000	70.8
trnS(tga)-petB_p2	trnS(tga)-petB	2	TGGTCGATTGCGTCTGATTT	TCGTTCTTCAAACCAATCGT	20	20	45.0	40.0	57.9	55.6	0.0	0.0	2.465782	32847	32934	191-193	1.000000	70.4
trnS(tga)-petB_p3	trnS(tga)-petB	3	TTGGTCGATTGCGTCTGATT	TCGTTCTTCAAACCAATCGT	20	20	45.0	40.0	57.9	55.6	0.0	0.0	2.465782	32847	32934	192-194	1.000000	70.3
trnS(tga)-petB_p4	trnS(tga)-petB	4	ATGGCAGAGTTGGTCGATTG	TCGTTCTTCAAACCAATCGT	20	20	50.0	40.0	58.3	55.6	0.0	0.0	2.629714	32847	32934	201-203	1.000000	68.9
trnS(tga)-petB_p5	trnS(tga)-petB	5	GGTCGATTGCGTCTGATTTG	TCGTTCTTCAAACCAATCGT	20	20	50.0	40.0	57.6	55.6	0.0	0.0	2.814665	32847	32934	190-192	1.000000	67.6
bas1_p1	bas1	1	AGAAGCTCCTGCAAAACCTG	ACGTTTCCGCGTTTAACTCT	20	20	50.0	45.0	58.4	58.1	0.0	4.9	0.529363	44326	44937	976-976	0.500000	55.8
bas1_p2	bas1	2	AGAAGCTCCTGCAAAACCTG	AGACGAGAATGTGCTCCTCT	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.540158	44326	44937	1042-1042	0.500000	55.7
bas1_p3	bas1	3	AGAAGCTCCTGCAAAACCTG	GACGTTTCCGCGTTTAACTC	20	20	50.0	50.0	58.4	57.8	0.0	4.9	0.588813	44326	44937	977-977	0.500000	55.3
bas1_p4	bas1	4	AGAAGCTCCTGCAAAACCTG	CGTTTCCGCGTTTAACTCTG	20	20	50.0	50.0	58.4	57.8	0.0	4.9	0.593205	44326	44937	975-975	0.500000	55.3
bas1_p5	bas1	5	AGAAGCTCCTGCAAAACCTG	TTCCGCGTTTAACTCTGACC	20	20	50.0	50.0	58.4	58.2	0.0	4.9	0.599152	44326	44937	972-972	0.500000	55.2
ycf45_p1	ycf45	1	GGTCAGAGTTAAACGCGGAA	CCTGCCGCCTCATCATTAAT	20	20	50.0	50.0	58.2	58.1	12.5	0.0	0.311397	45645	47024	2211-2211	0.500000	57.5
ycf45_p2	ycf45	2	GGTCAGAGTTAAACGCGGAA	AGGTGTAGATGGACGAAGCT	20	20	50.0	50.0	58.2	58.1	12.5	0.0	0.359025	45645	47024	2003-2003	0.500000	57.1
ycf45_p3	ycf45	3	ATGGTCAGAGTTAAACGCGG	CCTGCCGCCTCATCATTAAT	20	20	50.0	50.0	58.3	58.1	12.5	0.0	0.376398	45645	47024	2213-2213	0.500000	57.0
ycf45_p4	ycf45	4	GGTCAGAGTTAAACGCGGAA	GGTGTAACTTGGTCTGCCTC	20	20	50.0	55.0	58.2	58.2	12.5	0.0	0.403304	45645	47024	2103-2103	0.500000	56.8
ycf45_p5	ycf45	5	TGCGGCGTCAAAACTAAATG	CCTGCCGCCTCATCATTAAT	20	20	45.0	50.0	57.7	58.1	9.4	0.0	0.422349	45645	47024	2016-2016	0.500000	56.6
acpP_p1	acpP	1	AAGAAGCAAAACGACGAGGT	TGTCACTTCAAAAGCAGCCA	20	20	45.0	45.0	58.1	58.2	0.0	0.0	0.289086	47175	47411	1229-1229	0.500000	57.7
acpP_p2	acpP	2	AAGAAGCAAAACGACGAGGT	CGTTACTCCCAGTTGCTGAG	20	20	45.0	55.0	58.1	58.3	0.0	0.0	0.335740	47175	47411	1716-1716	0.500000	57.3
acpP_p3	acpP	3	AAGAAGCAAAACGACGAGGT	ATAGCGTCCTGGACCGTTAT	20	20	45.0	50.0	58.1	58.3	0.0	20.7	0.349580	47175	47411	1288-1288	0.500000	57.2
acpP_p4	acpP	4	AAGAAGCAAAACGACGAGGT	AGGTAACCGTTACTCCCAGT	20	20	45.0	50.0	58.1	57.7	0.0	18.6	0.366255	47175	47411	1723-1723	0.500000	57.1
acpP_p5	acpP	5	AGCTTCGTCCATCTACACCT	TGTCACTTCAAAAGCAGCCA	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.384588	47175	47411	529-529	0.500000	56.9
ycf89_p1	ycf89	1	ATTAATGATGAGGCGGCAGG	CATTGCGGAAAATTCCCCAC	20	20	50.0	50.0	58.1	58.0	13.1	6.6	0.107161	48078	48983	2184-2184	0.500000	59.1
ycf89_p2	ycf89	2	ATTAATGATGAGGCGGCAGG	ATATGGGGTTTTAGCAGCCG	20	20	50.0	50.0	58.1	58.0	13.1	0.0	0.117153	48078	48983	2002-2002	0.500000	59.1
ycf89_p3	ycf89	3	ATTAATGATGAGGCGGCAGG	TATTGCTCCGTCAGGCTTTC	20	20	50.0	50.0	58.1	58.0	13.1	0.0	0.122585	48078	48983	2207-2207	0.500000	59.0
ycf89_p4	ycf89	4	ATTAATGATGAGGCGGCAGG	TTTAGCAGCCGTTTCCAACT	20	20	50.0	45.0	58.1	58.0	13.1	0.0	0.122749	48078	48983	1993-1993	0.500000	59.0
ycf89_p5	ycf89	5	ATTAATGATGAGGCGGCAGG	TCGCCTTGGTAAGCCATTAC	20	20	50.0	50.0	58.1	58.0	13.1	0.0	0.131090	48078	48983	2087-2087	0.500000	59.0
rns_p1	rns	1	AAATCTCCCTGCTCTCTGGT	CGTCCTTCATCGCTTCTGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039723	49174	50657	2276-2276	0.500000	59.7
rns_p2	rns	2	AAATCTCCCTGCTCTCTGGT	TACTTGTGGAGGTAAGCGGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.108527	49174	50657	2167-2167	0.500000	59.1
rns_p3	rns	3	AAATCTCCCTGCTCTCTGGT	TCGCAGATTCACACGGAATT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.129321	49174	50657	2646-2646	0.500000	59.0
rns_p4	rns	4	AAATCTCCCTGCTCTCTGGT	AAAAGTTTAGGCTGGTCCCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.130377	49174	50657	2485-2485	0.500000	59.0
rns_p5	rns	5	AAATCTCCCTGCTCTCTGGT	TATGATTGTCCCGCTACCCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.133125	49174	50657	2508-2508	0.500000	58.9
rnl_p1	rnl	1	ACCTCCACAAGTAATAACAAA	AGCCTAAAATATTAACGATACAATTGT	21	27	33.3	25.9	52.7	56.0	0.0	16.7	15.310784	50934	53800	3000-3000	0.500000	20.0
rnl_p2	rnl	2	ACCTCCACAAGTAATAACAAAA	AGCCTAAAATATTAACGATACAATTGT	22	27	31.8	25.9	53.6	56.0	0.0	16.7	15.410006	50934	53800	3000-3000	0.500000	20.0
rnl_p3	rnl	3	ACCTCCACAAGTAATAACAAA	GCCTAAAATATTAACGATACAATTGT	21	26	33.3	26.9	52.7	54.8	0.0	16.7	15.541044	50934	53800	2999-2999	0.500000	20.0
rnl_p4	rnl	4	ACCTCCACAAGTAATAACAAAA	GCCTAAAATATTAACGATACAATTGT	22	26	31.8	26.9	53.6	54.8	0.0	16.7	15.640266	50934	53800	2999-2999	0.500000	20.0
rnl_p5	rnl	5	ACCTCCACAAGTAATAACAAA	AGCCTAAAATATTAACGATACAATTG	21	26	33.3	26.9	52.7	54.6	0.0	3.2	15.775976	50934	53800	3000-3000	0.500000	20.0
rrn5-psbA_p1	rrn5-psbA	1	GAAGCCCACCTCAAGATGAG	AACGCTCACAACTTCCCATT	20	20	55.0	45.0	58.0	58.0	0.3	0.0	0.054274	53986	54470	877-877	0.500000	59.6
rrn5-psbA_p2	rrn5-psbA	2	GAAGCCCACCTCAAGATGAG	TGCATCTTACATGGGTCGTG	20	20	55.0	50.0	58.0	58.0	0.3	0.0	0.058294	53986	54470	1511-1511	0.500000	59.5
rrn5-psbA_p3	rrn5-psbA	3	ATCTCCCCCAAGAGTTCACA	AACGCTCACAACTTCCCATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.063536	53986	54470	1182-1182	0.500000	59.5
rrn5-psbA_p4	rrn5-psbA	4	TCTCCCCCAAGAGTTCACAT	AACGCTCACAACTTCCCATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.063536	53986	54470	1181-1181	0.500000	59.5
rrn5-psbA_p5	rrn5-psbA	5	TCTCCCCCAAGAGTTCACAT	TGCATCTTACATGGGTCGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067557	53986	54470	1815-1815	0.500000	59.5
psbA_p1	psbA	1	CGCTGATCCATACCGAACTC	TCGGACAAGCAGTTTCACAA	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.259076	54471	55553	2001-2001	0.500000	57.9
psbA_p2	psbA	2	CGCTGATCCATACCGAACTC	GTTTCACAACGCTTACAGCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.260232	54471	55553	1990-1990	0.500000	57.9
psbA_p3	psbA	3	CGCTGATCCATACCGAACTC	ACACGTGGTGAAGAAGCAAT	20	20	55.0	45.0	58.2	58.0	0.0	21.6	0.260499	54471	55553	1957-1957	0.500000	57.9
psbA_p4	psbA	4	CGCTGATCCATACCGAACTC	TTGGAATTATCGCAGGTGGG	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.279152	54471	55553	2363-2363	0.500000	57.8
psbA_p5	psbA	5	CGCTGATCCATACCGAACTC	GAATTATCGCAGGTGGGGTT	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.280600	54471	55553	2360-2360	0.500000	57.8
psbA-rrn5_p1	psbA-rrn5	1	AACGCTCACAACTTCCCATT	GGGACCTCCTGGGAAGATAG	20	20	45.0	60.0	58.0	58.3	0.0	0.0	0.295455	95970	96454	599-679	1.000000	86.9
psbA-rrn5_p2	psbA-rrn5	2	GTAACGCTCACAACTTCCCA	GGGACCTCCTGGGAAGATAG	20	20	50.0	60.0	58.1	58.3	0.0	0.0	0.403014	95970	96454	601-681	1.000000	86.0
psbA-rrn5_p3	psbA-rrn5	3	TGGAAGTAATGCACGAACGT	GGGACCTCCTGGGAAGATAG	20	20	45.0	60.0	57.8	58.3	0.0	0.0	0.434546	95970	96454	619-699	1.000000	85.7
psbA-rrn5_p4	psbA-rrn5	4	AACGCTCACAACTTCCCATT	GGACCTCCTGGGAAGATAGT	20	20	45.0	55.0	58.0	57.5	0.0	0.0	0.499385	95970	96454	598-678	1.000000	85.3
psbA-rrn5_p5	psbA-rrn5	5	GTAATGCACGAACGTAACGC	GGGACCTCCTGGGAAGATAG	20	20	50.0	60.0	58.2	58.3	13.5	0.0	0.499202	95970	96454	614-694	1.000000	85.2
rnl-trnA(tgc)_p1	rnl-trnA(tgc)	1	CGTCCTTCATCGCTTCTGAA	GCGAGGTCTCTGGTTCAAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.104313	99507	99569	221-223	1.000000	83.5
rnl-trnA(tgc)_p2	rnl-trnA(tgc)	2	TCATCGCTTCTGAATGCCAA	GCGAGGTCTCTGGTTCAAAT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.212985	99507	99569	215-217	1.000000	82.7
rnl-trnA(tgc)_p3	rnl-trnA(tgc)	3	TTCATCGCTTCTGAATGCCA	GCGAGGTCTCTGGTTCAAAT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.212985	99507	99569	216-218	1.000000	82.7
rnl-trnA(tgc)_p4	rnl-trnA(tgc)	4	GTATCGTCGGTAACCACGTC	GCGAGGTCTCTGGTTCAAAT	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.285472	99507	99569	237-239	1.000000	81.7
rnl-trnA(tgc)_p5	rnl-trnA(tgc)	5	CGTCCTTCATCGCTTCTGAA	CTGATAAGGGCGAGGTCTCT	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.305202	99507	99569	230-232	1.000000	81.7
trnI(gat)-rns_p1	trnI(gat)-rns	1	ATTTGAACCAGAGACCTCGC	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.176584	99719	99782	181-186	1.000000	84.3
trnI(gat)-rns_p2	trnI(gat)-rns	2	CTGGGCCATTCTGGATTTGA	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	57.9	57.9	6.7	0.0	0.224425	99719	99782	195-200	1.000000	83.5
trnI(gat)-rns_p3	trnI(gat)-rns	3	ATTTGAACCAGAGACCTCGC	GGGGTGAAGTCGTAACAAGG	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.296004	99719	99782	177-182	1.000000	83.4
trnI(gat)-rns_p4	trnI(gat)-rns	4	AGAGACCTCGCCCTTATCAG	GACTGGGGTGAAGTCGTAAC	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.377473	99719	99782	172-177	1.000000	82.9
trnI(gat)-rns_p5	trnI(gat)-rns	5	CTGGGCCATTCTGGATTTGA	GGGGTGAAGTCGTAACAAGG	20	20	50.0	55.0	57.9	58.2	6.7	0.0	0.343845	99719	99782	191-196	1.000000	82.6
ycf89_p1	ycf89	1	CATTGCGGAAAATTCCCCAC	ATTAATGATGAGGCGGCAGG	20	20	50.0	50.0	58.0	58.1	6.6	13.1	0.107161	101457	102362	2184-2184	0.500000	59.1
ycf89_p2	ycf89	2	ATATGGGGTTTTAGCAGCCG	ATTAATGATGAGGCGGCAGG	20	20	50.0	50.0	58.0	58.1	0.0	13.1	0.117153	101457	102362	2002-2002	0.500000	59.1
ycf89_p3	ycf89	3	TATTGCTCCGTCAGGCTTTC	ATTAATGATGAGGCGGCAGG	20	20	50.0	50.0	58.0	58.1	0.0	13.1	0.122585	101457	102362	2207-2207	0.500000	59.0
ycf89_p4	ycf89	4	TTTAGCAGCCGTTTCCAACT	ATTAATGATGAGGCGGCAGG	20	20	45.0	50.0	58.0	58.1	0.0	13.1	0.122749	101457	102362	1993-1993	0.500000	59.0
ycf89_p5	ycf89	5	TCGCCTTGGTAAGCCATTAC	ATTAATGATGAGGCGGCAGG	20	20	50.0	50.0	58.0	58.1	0.0	13.1	0.131090	101457	102362	2087-2087	0.500000	59.0
trnP(tgg)-acpP_p1	trnP(tgg)-acpP	1	AGCTGCTCGCATTTGACATA	ATTAATGATGAGGCGGCAGG	20	20	45.0	50.0	58.0	58.1	0.0	13.1	0.126829	102534	103028	1071-1071	0.500000	59.0
trnP(tgg)-acpP_p2	trnP(tgg)-acpP	2	AGCTGCTCGCATTTGACATA	AGCTTCGTCCATCTACACCT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.174457	102534	103028	1279-1279	0.500000	58.6
trnP(tgg)-acpP_p3	trnP(tgg)-acpP	3	AAAACTCCGGTAAATGCGGT	ATTAATGATGAGGCGGCAGG	20	20	45.0	50.0	58.1	58.1	0.0	13.1	0.198164	102534	103028	1166-1166	0.500000	58.4
trnP(tgg)-acpP_p4	trnP(tgg)-acpP	4	AAACTCCGGTAAATGCGGTT	ATTAATGATGAGGCGGCAGG	20	20	45.0	50.0	58.1	58.1	0.0	13.1	0.198164	102534	103028	1165-1165	0.500000	58.4
trnP(tgg)-acpP_p5	trnP(tgg)-acpP	5	AGCTGCTCGCATTTGACATA	GAGGCAGACCAAGTTACACC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.218736	102534	103028	1179-1179	0.500000	58.3