primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	79	393	384-390	1.000000	87.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	79	393	398-404	1.000000	86.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	79	393	397-403	1.000000	86.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	79	393	399-405	1.000000	86.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGAGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	57.8	0.0	0.0	0.369413	79	393	433-439	1.000000	84.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.081289	6340	6942	683-812	1.000000	91.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	0.0	16.9	0.210236	6340	6942	685-814	1.000000	90.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	6340	6942	671-800	1.000000	89.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.293454	6340	6942	686-815	1.000000	89.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.4	58.1	0.0	16.9	0.428755	6340	6942	684-813	1.000000	88.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.793395	10466	10755	408-493	1.000000	68.8
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	21	20	47.6	50.0	57.3	57.5	0.0	0.0	2.165486	10466	10755	410-495	1.000000	65.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TGGATAGGACAGAGGTCTTCT	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	56.9	57.9	0.0	0.0	2.152209	10466	10755	443-528	1.000000	65.7
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTCACTCGGGA	21	20	47.6	50.0	57.3	58.7	0.0	0.0	2.344727	10466	10755	411-496	1.000000	64.4
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GTTCAAATCCTATTGGACGCA	AAGACATTCACTCGGGAAGC	21	20	42.9	50.0	56.6	57.9	0.0	0.0	2.496339	10466	10755	407-492	1.000000	63.2
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	20	20	55.0	50.0	58.0	57.7	0.0	29.6	0.338107	38737	39259	678-705	1.000000	81.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	57.8	57.7	0.0	29.6	0.527394	38737	39259	681-708	1.000000	79.5
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	57.8	57.7	0.0	29.6	0.547753	38737	39259	674-701	1.000000	79.3
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	20	20	55.0	50.0	58.3	57.7	0.0	29.6	0.578376	38737	39259	679-706	1.000000	79.1
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTCTCCTGATGGTTGGTCGA	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	58.4	57.7	0.0	29.6	0.682649	38737	39259	677-704	1.000000	78.2
ycf1_p1	ycf1	1	ATCGTTCCATAGCGAATCGG	TCAAGATTTACTCGGCCTGC	20	20	50.0	50.0	57.9	58.0	4.4	0.0	0.090168	116464	116583	247-247	0.500000	59.3
ycf1_p2	ycf1	2	ATCGTTCCATAGCGAATCGG	TTTACTCGGCCTGCGTATTC	20	20	50.0	50.0	57.9	58.1	4.4	0.0	0.135425	116464	116583	241-241	0.500000	58.9
ycf1_p3	ycf1	3	TGGATTTTGGGGCTTGGATC	TCAAGATTTACTCGGCCTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.159507	116464	116583	364-364	0.500000	58.7
ycf1_p4	ycf1	4	AGATCGCCATTCCAAGCAAA	TCAAGATTTACTCGGCCTGC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.186063	116464	116583	560-560	0.500000	58.5
ycf1_p5	ycf1	5	TGGATTTTGGGGCTTGGATC	TTTACTCGGCCTGCGTATTC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.204764	116464	116583	358-358	0.500000	58.4
ndhH_p1	ndhH	1	GCAGCCGTTTCAGTATGGTA	CCGTCTGCTAGAGCTTGAAG	20	20	50.0	55.0	58.0	58.1	0.0	2.7	0.098624	118132	119313	2069-2069	0.500000	59.2
ndhH_p2	ndhH	2	GCAGCCGTTTCAGTATGGTA	TTTGTTCCGTCTGCTAGAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.098828	118132	119313	2075-2075	0.500000	59.2
ndhH_p3	ndhH	3	GCAGCCGTTTCAGTATGGTA	TCAGGTCCAATACGCTGTTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.111848	118132	119313	2027-2027	0.500000	59.1
ndhH_p4	ndhH	4	GAAAACCCGGGATCGATTGA	CCGTCTGCTAGAGCTTGAAG	20	20	50.0	55.0	58.0	58.1	32.0	2.7	0.112762	118132	119313	1747-1747	0.500000	59.1
ndhH_p5	ndhH	5	GAAAACCCGGGATCGATTGA	TTTGTTCCGTCTGCTAGAGC	20	20	50.0	50.0	58.0	57.9	32.0	0.0	0.112966	118132	119313	1753-1753	0.500000	59.1
ndhA_p1	ndhA	1	GTGAGAGTTGAAGCCCCAAA	CGATTTGCGTGGGATAAGGT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.355049	119315	121641	2712-2712	0.500000	57.2
ndhA_p2	ndhA	2	CAGGCTCTGGAAGGAATTCC	CGATTTGCGTGGGATAAGGT	20	20	55.0	50.0	58.0	58.3	17.3	0.0	0.386194	119315	121641	2865-2865	0.500000	56.9
ndhA_p3	ndhA	3	GTGAGAGTTGAAGCCCCAAA	TTGACCAATGTACCTTGCGG	20	20	50.0	50.0	58.0	58.5	0.0	5.4	0.489518	119315	121641	2680-2680	0.500000	56.1
ndhA_p4	ndhA	4	CAGGCTCTGGAAGGAATTCC	TTGACCAATGTACCTTGCGG	20	20	55.0	50.0	58.0	58.5	17.3	5.4	0.520663	119315	121641	2833-2833	0.500000	55.8
ndhA_p5	ndhA	5	GTGAGAGTTGAAGCCCCAAA	CGGCTCGTACTGTTTGTTGA	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.524972	119315	121641	2663-2663	0.500000	55.8
ndhA-ndhI_p1	ndhA-ndhI	1	ACAGTTCTTTACTTGGGCGG	CGGCCCAAAGCAATTTGATT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.205470	121642	121720	789-789	0.500000	58.4
ndhA-ndhI_p2	ndhA-ndhI	2	CAGTTCTTTACTTGGGCGGT	CGGCCCAAAGCAATTTGATT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.205470	121642	121720	788-788	0.500000	58.4
ndhA-ndhI_p3	ndhA-ndhI	3	ATTTATGGCCTTCGAGGAGC	CGGCCCAAAGCAATTTGATT	20	20	50.0	45.0	57.7	57.9	3.3	0.0	0.360103	121642	121720	1141-1141	0.500000	57.1
ndhA-ndhI_p4	ndhA-ndhI	4	TTTACTTGGGCGGTTGGAAT	CGGCCCAAAGCAATTTGATT	20	20	45.0	45.0	57.7	57.9	0.0	0.0	0.393752	121642	121720	782-782	0.500000	56.8
ndhA-ndhI_p5	ndhA-ndhI	5	CAGTTCTTTACTTGGGCGGT	ACATTGGTAAACGGCCCAAA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.401918	121642	121720	799-799	0.500000	56.8
ndhI_p1	ndhI	1	ACAGTTCTTTACTTGGGCGG	GTGCTGCAGCTACAAAATGG	20	20	50.0	50.0	58.1	58.0	0.0	36.6	0.121093	121721	122206	1396-1396	0.500000	59.0
ndhI_p2	ndhI	2	CAGTTCTTTACTTGGGCGGT	GTGCTGCAGCTACAAAATGG	20	20	50.0	50.0	58.1	58.0	0.0	36.6	0.121093	121721	122206	1395-1395	0.500000	59.0
ndhI_p3	ndhI	3	ACAGTTCTTTACTTGGGCGG	CAAGAACCAGTCCCAACGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.145997	121721	122206	1342-1342	0.500000	58.8
ndhI_p4	ndhI	4	CAGTTCTTTACTTGGGCGGT	CAAGAACCAGTCCCAACGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.145997	121721	122206	1341-1341	0.500000	58.8
ndhI_p5	ndhI	5	CAGTTCTTTACTTGGGCGGT	TCCCAACGAAAAGGCAGAAA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.167841	121721	122206	1331-1331	0.500000	58.7
ndhG_p1	ndhG	1	AATCAAATTGCTTTGGGCCG	TTGACGAAACAATAGCCGGT	20	20	45.0	45.0	57.9	57.8	0.0	1.9	0.270531	122575	123105	1459-1459	0.500000	57.8
ndhG_p2	ndhG	2	AATCAAATTGCTTTGGGCCG	ACAATAGCCGGTCCAATAGC	20	20	45.0	50.0	57.9	57.7	0.0	1.9	0.353797	122575	123105	1451-1451	0.500000	57.2
ndhG_p3	ndhG	3	AATCAAATTGCTTTGGGCCG	TGACGAAACAATAGCCGGTC	20	20	45.0	50.0	57.9	58.3	0.0	1.9	0.380486	122575	123105	1458-1458	0.500000	57.0
ndhG_p4	ndhG	4	AATCAAATTGCTTTGGGCCG	TTTCGGCTCGTGATCAATCC	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.445692	122575	123105	1286-1286	0.500000	56.4
ndhG_p5	ndhG	5	TTTGGGCCGTTTACCAATGT	TTGACGAAACAATAGCCGGT	20	20	45.0	45.0	58.3	57.8	0.0	1.9	0.466978	122575	123105	1448-1448	0.500000	56.3
ndhE_p1	ndhE	1	CCATTTTGTAGCTGCAGCAC	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	58.0	58.0	38.2	12.1	0.028758	123341	123646	1312-1312	0.500000	59.8
ndhE_p2	ndhE	2	CCATTTTGTAGCTGCAGCAC	ACCCATGCTTCGAGTTGTTT	20	20	50.0	45.0	58.0	58.0	38.2	0.0	0.032046	123341	123646	1406-1406	0.500000	59.7
ndhE_p3	ndhE	3	CCATTTTGTAGCTGCAGCAC	GCTTCGAGTTGTTTCATGCC	20	20	50.0	50.0	58.0	58.0	38.2	0.0	0.044199	123341	123646	1400-1400	0.500000	59.6
ndhE_p4	ndhE	4	TTCGTTGGGACTGGTTCTTG	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	58.0	58.0	0.0	12.1	0.053663	123341	123646	1366-1366	0.500000	59.6
ndhE_p5	ndhE	5	TTCGTTGGGACTGGTTCTTG	ACCCATGCTTCGAGTTGTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056950	123341	123646	1460-1460	0.500000	59.5
psaC_p1	psaC	1	ACCGGCTATTGTTTCGTCAA	AACCGATACCGAGAACTCCT	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.313690	123911	124156	1270-1270	0.500000	57.5
psaC_p2	psaC	2	GCTATTGGACCGGCTATTGT	AACCGATACCGAGAACTCCT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.396956	123911	124156	1278-1278	0.500000	56.8
psaC_p3	psaC	3	GACCGGCTATTGTTTCGTCA	AACCGATACCGAGAACTCCT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.423645	123911	124156	1271-1271	0.500000	56.6
psaC_p4	psaC	4	GGATTGATCACGAGCCGAAA	AACCGATACCGAGAACTCCT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.488851	123911	124156	1443-1443	0.500000	56.1
psaC_p5	psaC	5	ACCGGCTATTGTTTCGTCAA	AAGAAACCCGCGGATATTGG	20	20	45.0	50.0	57.8	58.3	0.0	30.7	0.497299	123911	124156	770-770	0.500000	56.0
ndhD_p1	ndhD	1	TCTGCTCCAAGAACAGAGGA	TCAGGAGCAGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.190142	124282	125778	2286-2286	0.500000	58.5
ndhD_p2	ndhD	2	TCTGCTCCAAGAACAGAGGA	TTCAGGAGCAGTATGGGCTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.190142	124282	125778	2287-2287	0.500000	58.5
ndhD_p3	ndhD	3	AAACAACTCGAAGCATGGGT	TCAGGAGCAGTATGGGCTAA	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.193429	124282	125778	2192-2192	0.500000	58.5
ndhD_p4	ndhD	4	AAACAACTCGAAGCATGGGT	TTCAGGAGCAGTATGGGCTA	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.193429	124282	125778	2193-2193	0.500000	58.5
ndhD_p5	ndhD	5	GGCATGAAACAACTCGAAGC	TCAGGAGCAGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.205582	124282	125778	2198-2198	0.500000	58.4
ccsA_p1	ccsA	1	GGTGTTTGTGAGAACCCCTT	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.067717	126071	127042	1770-1770	0.500000	59.5
ccsA_p2	ccsA	2	GGTGTTTGTGAGAACCCCTT	AGGGATATTGGGCAGCATTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.072736	126071	127042	1771-1771	0.500000	59.4
ccsA_p3	ccsA	3	GGTGTTTGTGAGAACCCCTT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.075196	126071	127042	1288-1288	0.500000	59.4
ccsA_p4	ccsA	4	GGTGTTTGTGAGAACCCCTT	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.076566	126071	127042	1287-1287	0.500000	59.4
ccsA_p5	ccsA	5	CAGTTGACAAGGTCGAAGCT	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.129272	126071	127042	1961-1961	0.500000	59.0
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.008182	127226	127598	502-502	0.500000	59.9
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009553	127226	127598	503-503	0.500000	59.9
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	AGGGATATTGGGCAGCATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	127226	127598	503-503	0.500000	59.9
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	AGGGATATTGGGCAGCATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014571	127226	127598	504-504	0.500000	59.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GCTGCATAGCCCAATACCAT	GGGATATTGGGCAGCATTGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.085211	127226	127598	1108-1108	0.500000	59.3
rpl32-ndhF_p1	rpl32-ndhF	1	TCAATGCTGCCCAATATCCC	TAGGGCGTGTGAACCAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.054584	127767	128524	1354-1354	0.500000	59.6
rpl32-ndhF_p2	rpl32-ndhF	2	CAATGCTGCCCAATATCCCT	TAGGGCGTGTGAACCAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.059602	127767	128524	1353-1353	0.500000	59.5
rpl32-ndhF_p3	rpl32-ndhF	3	AGCGTGTCTACCGATTTCAC	TAGGGCGTGTGAACCAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.062062	127767	128524	1836-1836	0.500000	59.5
rpl32-ndhF_p4	rpl32-ndhF	4	CAGCGTGTCTACCGATTTCA	TAGGGCGTGTGAACCAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.063433	127767	128524	1837-1837	0.500000	59.5
rpl32-ndhF_p5	rpl32-ndhF	5	TCAATGCTGCCCAATATCCC	AGCAACTGAACCGCCAAATA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.096065	127767	128524	1563-1563	0.500000	59.2
ndhF_p1	ndhF	1	TCCTGCTTACTTGGTCGTTT	GTTTCGATGCAACAACCAACT	20	21	45.0	42.9	57.1	57.9	0.0	0.0	2.001334	128525	130813	2950-2950	0.500000	44.0
ndhF_p2	ndhF	2	TCCTGCTTACTTGGTCGTTT	TGTTTCGATGCAACAACCAAC	20	21	45.0	42.9	57.1	58.2	0.0	0.0	2.119289	128525	130813	2951-2951	0.500000	43.0
ndhF_p3	ndhF	3	TCCTGCTTACTTGGTCGTTT	TGTTTCGATGCAACAACCAA	20	20	45.0	40.0	57.1	56.8	0.0	0.0	2.158060	128525	130813	2951-2951	0.500000	42.7
ndhF_p4	ndhF	4	ATCCTGCTTACTTGGTCGTT	GTTTCGATGCAACAACCAACT	20	21	45.0	42.9	56.8	57.9	0.0	0.0	2.230726	128525	130813	2951-2951	0.500000	42.2
ndhF_p5	ndhF	5	ATCCTGCTTACTTGGTCGTT	TGTTTCGATGCAACAACCAAC	20	21	45.0	42.9	56.8	58.2	0.0	0.0	2.348682	128525	130813	2952-2952	0.500000	41.2