primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbI-trnS-GCU_p1 psbI-trnS-GCU 1 TCCTATCTAACGATCCGGGAC ATTTTCGTACCGAGGGTTCG 21 20 52.4 50.0 58.2 58.0 0.0 7.4 1.212435 8133 8445 301-393 1.000000 80.2 psbI-trnS-GCU_p2 psbI-trnS-GCU 2 TCCTATCTAACGATCCGGGA ATTTTCGTACCGAGGGTTCG 20 20 50.0 50.0 56.7 58.0 0.0 7.4 1.260133 8133 8445 301-393 1.000000 79.8 psbI-trnS-GCU_p3 psbI-trnS-GCU 3 TCCTATCTAACGATCCGGGAC TGGACTAAAGCGTCGGATTG 21 20 52.4 50.0 58.2 58.0 0.0 0.0 1.216588 8133 8445 338-430 1.000000 79.6 psbI-trnS-GCU_p4 psbI-trnS-GCU 4 TCCTATCTAACGATCCGGGAC GTGGACTAAAGCGTCGGATT 21 20 52.4 50.0 58.2 58.0 0.0 0.0 1.217976 8133 8445 339-431 1.000000 79.6 psbI-trnS-GCU_p5 psbI-trnS-GCU 5 TCCTATCTAACGATCCGGGA TGGACTAAAGCGTCGGATTG 20 20 50.0 50.0 56.7 58.0 0.0 0.0 1.264286 8133 8445 338-430 1.000000 79.2 atpF-atpH_p1 atpF-atpH 1 AAAGAAAGGAAAGAATGGGT AGGCAGAGGGAAAAATACGG 20 20 35.0 50.0 52.5 57.6 0.0 0.0 5.931095 13712 14482 752-997 1.000000 52.0 atpF-atpH_p2 atpF-atpH 2 AAGAAAGGAAAGAATGGGTT AGGCAGAGGGAAAAATACGG 20 20 35.0 50.0 52.5 57.6 0.0 0.0 5.931095 13712 14482 751-996 1.000000 52.0 atpF-atpH_p3 atpF-atpH 3 AAAGAAAGGAAAGAATGGGTT AGGCAGAGGGAAAAATACGG 21 20 33.3 50.0 53.4 57.6 0.0 0.0 5.972518 13712 14482 752-997 1.000000 52.0 atpF-atpH_p4 atpF-atpH 4 AAAGAAAGGAAAGAATGGGT CCTGAGGCAGAGGGAAAAAT 20 20 35.0 50.0 52.5 57.5 0.0 0.0 6.032584 13712 14482 756-1001 1.000000 52.0 atpF-atpH_p5 atpF-atpH 5 AAGAAAGGAAAGAATGGGTT CCTGAGGCAGAGGGAAAAAT 20 20 35.0 50.0 52.5 57.5 0.0 0.0 6.032584 13712 14482 755-1000 1.000000 52.0 atpH-atpI_p1 atpH-atpI 1 AATAGAAGCAAGCCCGACAG TATGGTCCATGGGTTCCCTT 20 20 50.0 50.0 58.0 58.0 0.0 15.1 0.035440 14729 16134 1763-2064 1.000000 87.4 atpH-atpI_p2 atpH-atpI 2 ATAACGGAAGCGGCAGAAAT TATGGTCCATGGGTTCCCTT 20 20 45.0 50.0 58.0 58.0 1.4 15.1 0.043988 14729 16134 1729-2030 1.000000 87.4 atpH-atpI_p3 atpH-atpI 3 AGGAAGAAGGCGAGTCGATA TATGGTCCATGGGTTCCCTT 20 20 50.0 50.0 57.9 58.0 18.6 15.1 0.059833 14729 16134 2128-2417 1.000000 86.9 atpH-atpI_p4 atpH-atpI 4 AAGCGCTAATGCTACAACCA TATGGTCCATGGGTTCCCTT 20 20 45.0 50.0 57.9 58.0 12.1 15.1 0.113551 14729 16134 1919-2220 1.000000 86.5 atpH-atpI_p5 atpH-atpI 5 GGGCTCGTTGGACTAACAAT TATGGTCCATGGGTTCCCTT 20 20 50.0 50.0 57.9 58.0 2.4 15.1 0.115026 14729 16134 2164-2453 1.000000 86.4 atpI-rps2_p1 atpI-rps2 1 CTTGGGCATGGACTTGGAAA TGCAATTTGTGAGGGTCGTT 20 20 50.0 45.0 58.4 58.3 0.0 0.0 0.682240 16879 17161 397-441 1.000000 80.9 atpI-rps2_p2 atpI-rps2 2 CTTGGGCATGGACTTGGAAA TTGCAATTTGTGAGGGTCGT 20 20 50.0 45.0 58.4 58.3 0.0 0.0 0.682240 16879 17161 398-442 1.000000 80.9 atpI-rps2_p3 atpI-rps2 3 ATGTTGGCCTACTTCCACAG TGCAATTTGTGAGGGTCGTT 20 20 50.0 45.0 57.5 58.3 18.3 0.0 0.810544 16879 17161 357-401 1.000000 80.1 atpI-rps2_p4 atpI-rps2 4 ATGTTGGCCTACTTCCACAG TTGCAATTTGTGAGGGTCGT 20 20 50.0 45.0 57.5 58.3 18.3 0.0 0.810544 16879 17161 358-402 1.000000 80.1 atpI-rps2_p5 atpI-rps2 5 AGTACTTGGGCATGGACTTG TGCAATTTGTGAGGGTCGTT 20 20 50.0 45.0 57.5 58.3 0.0 0.0 0.810544 16879 17161 401-445 1.000000 79.8 trnC-GCA-petN_p1 trnC-GCA-petN 1 ACGCACGATAATTTCCCGAA AGCCCAAGCGAGACTTACTA 20 20 45.0 50.0 58.0 58.2 0.0 0.0 0.164285 29709 30618 1173-1378 1.000000 90.4 trnC-GCA-petN_p2 trnC-GCA-petN 2 TTCGTTCGGGACAGCATATG AGCCCAAGCGAGACTTACTA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.216760 29709 30618 1248-1453 1.000000 89.7 trnC-GCA-petN_p3 trnC-GCA-petN 3 ACGCACGATAATTTCCCGAA AACCTTTTCCCTCCCTCCTT 20 20 45.0 50.0 58.0 58.2 0.0 0.0 0.164762 29709 30618 1533-1763 1.000000 89.5 trnC-GCA-petN_p4 trnC-GCA-petN 4 ACGCACGATAATTTCCCGAA ACCTTTTCCCTCCCTCCTTT 20 20 45.0 50.0 58.0 58.2 0.0 0.0 0.164762 29709 30618 1532-1762 1.000000 89.5 trnC-GCA-petN_p5 trnC-GCA-petN 5 TTCGTTCGGGACAGCATATG ACCTTTTCCCTCCCTCCTTT 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.217237 29709 30618 1607-1837 1.000000 88.8 psbC-trnS-UGA_p1 psbC-trnS-UGA 1 AGCTGCAGCAGGATTTGAAA GAGAGATGGCTGAGTGGTTG 20 20 45.0 55.0 58.4 58.0 38.2 0.0 0.397019 38467 38699 394-400 1.000000 82.1 psbC-trnS-UGA_p2 psbC-trnS-UGA 2 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 20 20 45.0 55.0 58.4 58.1 38.2 0.0 0.434339 38467 38699 379-385 1.000000 82.0 psbC-trnS-UGA_p3 psbC-trnS-UGA 3 AGCTGCAGCAGGATTTGAAA GCTGAGTGGTTGATAGCTCC 20 20 45.0 55.0 58.4 58.1 38.2 0.0 0.428919 38467 38699 386-392 1.000000 81.9 psbC-trnS-UGA_p4 psbC-trnS-UGA 4 AGCTGCAGCAGGATTTGAAA GGCTGAGTGGTTGATAGCTC 20 20 45.0 55.0 58.4 58.1 38.2 0.0 0.428919 38467 38699 387-393 1.000000 81.9 psbC-trnS-UGA_p5 psbC-trnS-UGA 5 TAGGTCATTTATGGCACGCG GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 58.4 58.0 3.5 0.0 0.443887 38467 38699 428-434 1.000000 81.3 psbZ-trnG-UCC_p1 psbZ-trnG-UCC 1 CTTCTCCTGATGGTTGGTCG CGATCATAGGAGGGTCCCTT 20 20 55.0 55.0 58.0 58.0 0.0 13.1 0.032641 39375 40090 980-1449 1.000000 91.7 psbZ-trnG-UCC_p2 psbZ-trnG-UCC 2 CTTCTCCTGATGGTTGGTCG CTTGAGGTCACGGGTTCAAA 20 20 55.0 50.0 58.0 58.0 0.0 4.2 0.053942 39375 40090 822-1291 1.000000 91.6 psbZ-trnG-UCC_p3 psbZ-trnG-UCC 3 CTTCTCCTGATGGTTGGTCG CGACGTTGTGTTTTAACCGG 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.023617 39375 40090 1147-1616 1.000000 90.9 psbZ-trnG-UCC_p4 psbZ-trnG-UCC 4 CAACCACTCAGCCATCTCTC CGATCATAGGAGGGTCCCTT 20 20 55.0 55.0 58.0 58.0 0.0 13.1 0.038260 39375 40090 1455-1937 1.000000 90.1 psbZ-trnG-UCC_p5 psbZ-trnG-UCC 5 CAACCACTCAGCCATCTCTC CGACGTTGTGTTTTAACCGG 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.029236 39375 40090 1622-2104 1.000000 89.7 rps4-trnT-UGU_p1 rps4-trnT-UGU 1 TAAAGACCCCAGACGACGTA GGTTCGATTCCGATAGCCG 20 19 50.0 57.9 57.8 58.2 0.0 0.0 1.388341 49624 50102 414-611 1.000000 77.7 rps4-trnT-UGU_p2 rps4-trnT-UGU 2 GAAACGAGGCCCTCTGTAAC GGTTCGATTCCGATAGCCG 20 19 55.0 57.9 58.3 58.2 9.5 0.0 1.466295 49624 50102 387-584 1.000000 77.3 rps4-trnT-UGU_p3 rps4-trnT-UGU 3 TTTTTGAAACGAGGCCCTCT GGTTCGATTCCGATAGCCG 20 19 45.0 57.9 57.6 58.2 9.5 0.0 1.547836 49624 50102 392-589 1.000000 76.6 rps4-trnT-UGU_p4 rps4-trnT-UGU 4 TTTTGAAACGAGGCCCTCTG GGTTCGATTCCGATAGCCG 20 19 50.0 57.9 58.4 58.2 9.5 0.0 1.584910 49624 50102 391-588 1.000000 76.4 rps4-trnT-UGU_p5 rps4-trnT-UGU 5 GACCCCAGACGACGTATTTT GGTTCGATTCCGATAGCCG 20 19 50.0 57.9 57.6 58.2 0.0 0.0 1.564687 49624 50102 410-607 1.000000 76.3 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 50176 50992 668-970 1.000000 89.6 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 50176 50992 674-976 1.000000 88.2 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG TACCAATTTCGCCATACCCC 20 20 55.0 50.0 57.9 57.6 0.0 0.0 0.484446 50176 50992 661-963 1.000000 87.4 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GCGATGCTCTAACCTCTGAG TAAGTCCGTAGCGTCTACCA 20 20 55.0 50.0 57.9 57.6 0.0 10.2 0.534323 50176 50992 676-978 1.000000 86.9 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 CTAACCTCTGAGCTAAGCGG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.5 58.1 5.5 0.0 0.569941 50176 50992 660-962 1.000000 86.7 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 GGGATAGCTCAGCTGGTAGA ATGCCCGAAAGTTGGATAGG 20 20 55.0 50.0 58.0 57.7 10.3 0.0 0.350916 52199 52984 731-937 1.000000 84.3 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 CCTCGTGTCACCAGTTCAAA ATGCCCGAAAGTTGGATAGG 20 20 50.0 50.0 58.1 57.7 0.0 0.0 0.400307 52199 52984 695-901 1.000000 84.0 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 AGAATGGTCGGGATAGCTCA ATGCCCGAAAGTTGGATAGG 20 20 50.0 50.0 57.9 57.7 0.0 0.0 0.436515 52199 52984 740-946 1.000000 83.6 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 CTGGTAGAGCAGAGGACTGA ATGCCCGAAAGTTGGATAGG 20 20 55.0 50.0 57.9 57.7 2.5 0.0 0.484709 52199 52984 719-925 1.000000 83.3 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 GGGATAGCTCAGCTGGTAGA GCCCGAAAGTTGGATAGGAT 20 20 55.0 50.0 58.0 57.4 10.3 0.0 0.642821 52199 52984 729-935 1.000000 82.0 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 CATCATATTCATAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 24 20 25.0 55.0 52.7 58.7 0.0 0.0 9.982368 54715 55196 464-543 1.000000 49.4 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 CATCATATTCATAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 24 20 25.0 55.0 52.7 57.5 0.0 0.0 9.840074 54715 55196 475-554 1.000000 49.3 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 CATCATATTCATAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 24 20 25.0 50.0 52.7 57.8 0.0 0.0 9.496302 54715 55196 476-555 1.000000 49.2 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 ACATCATATTCATAAAGCAAAAAC AGAAGGTCTACGGTTCGAGT 24 20 25.0 50.0 52.7 57.8 0.0 0.0 9.500345 54715 55196 477-556 1.000000 49.2 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 ACATCATATTCATAAAGCAAAAAC GAAGGTCTACGGTTCGAGTC 24 20 25.0 55.0 52.7 57.5 0.0 0.0 9.844117 54715 55196 476-555 1.000000 49.2 rbcL-accD_p1 rbcL-accD 1 AGTCCTGAATTAGCTGCTGC TTAGTTAGCCCACACCCGTA 20 20 50.0 50.0 58.0 58.1 13.4 0.0 0.099731 60510 61410 1044-1138 1.000000 87.3 rbcL-accD_p2 rbcL-accD 2 GAGGCTAGCAAATGGAGTCC TTAGTTAGCCCACACCCGTA 20 20 55.0 50.0 58.0 58.1 18.2 0.0 0.103784 60510 61410 1059-1153 1.000000 87.2 rbcL-accD_p3 rbcL-accD 3 AGTCCTGAATTAGCTGCTGC TAGTTAGCCCACACCCGTAT 20 20 50.0 50.0 58.0 57.8 13.4 0.0 0.199340 60510 61410 1043-1137 1.000000 86.5 rbcL-accD_p4 rbcL-accD 4 GAGGCTAGCAAATGGAGTCC TAGTTAGCCCACACCCGTAT 20 20 55.0 50.0 58.0 57.8 18.2 0.0 0.203393 60510 61410 1058-1152 1.000000 86.4 rbcL-accD_p5 rbcL-accD 5 AGTCCTGAATTAGCTGCTGC GCCCACACCCGTATTCTAAC 20 20 50.0 55.0 58.0 58.3 13.4 0.0 0.375363 60510 61410 1037-1131 1.000000 85.1 rps3-rps19_p1 rps3-rps19 1 TACCAAGTCTGAAACCGAGC TCTAAACTTTCGGGGACACG 20 20 50.0 50.0 57.5 57.6 0.0 0.0 0.894497 88006 88787 780-892 1.000000 84.6 rps3-rps19_p2 rps3-rps19 2 GTCTGAAACCGAGCGGATTT TCTAAACTTTCGGGGACACG 20 20 50.0 50.0 58.6 57.6 16.8 0.0 1.004209 88006 88787 774-886 1.000000 83.8 rps3-rps19_p3 rps3-rps19 3 TCTGAAACCGAGCGGATTTA TCTAAACTTTCGGGGACACG 20 20 45.0 50.0 57.0 57.6 16.8 0.0 1.486461 88006 88787 773-885 1.000000 79.9 rps3-rps19_p4 rps3-rps19 4 TACCAAGTCTGAAACCGAGC TAAACTTTCGGGGACACGC 20 19 50.0 52.6 57.5 58.1 0.0 0.0 1.533438 88006 88787 778-890 1.000000 79.5 rps3-rps19_p5 rps3-rps19 5 TACCAAGTCTGAAACCGAGC GGGGACACGCCAAAAATGAT 20 20 50.0 50.0 57.5 59.1 0.0 0.0 1.555774 88006 88787 769-881 1.000000 79.4 trnR-ACG-trnN-GUU_p1 trnR-ACG-trnN-GUU 1 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 20 20 55.0 50.0 57.8 58.1 18.9 0.0 0.311620 113034 113731 758-906 1.000000 85.2 trnR-ACG-trnN-GUU_p2 trnR-ACG-trnN-GUU 2 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 20 20 55.0 50.0 57.8 57.8 18.9 15.9 0.366304 113034 113731 681-829 1.000000 85.2 trnR-ACG-trnN-GUU_p3 trnR-ACG-trnN-GUU 3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 20 20 55.0 50.0 57.8 58.2 18.9 18.9 0.372539 113034 113731 668-816 1.000000 85.2 trnR-ACG-trnN-GUU_p4 trnR-ACG-trnN-GUU 4 GAACAGATCCAGTGGAGACG TGGGTTGAGAACGGGAATTG 20 20 55.0 50.0 57.7 58.1 18.7 0.0 0.381540 113034 113731 795-943 1.000000 84.5 trnR-ACG-trnN-GUU_p5 trnR-ACG-trnN-GUU 5 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 20 20 55.0 50.0 57.8 57.8 18.9 0.0 0.410108 113034 113731 757-905 1.000000 84.5 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GCCACATCTCTTTCTACGGG AGCGTGTCTACCGATTTCAC 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.066505 118194 119380 1225-1330 1.000000 87.7 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 GCCACATCTCTTTCTACGGG CAGCGTGTCTACCGATTTCA 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.067876 118194 119380 1226-1331 1.000000 87.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 ACGGGGAACTCAAAAAGCTT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.075196 118194 119380 1210-1315 1.000000 87.7 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 ACGGGGAACTCAAAAAGCTT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.076566 118194 119380 1211-1316 1.000000 87.7 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GCCACATCTCTTTCTACGGG GCTGCATAGCCCAATACCAT 20 20 55.0 50.0 58.1 58.1 0.0 0.0 0.143534 118194 119380 1825-1925 1.000000 86.2 rps15-ycf1_p1 rps15-ycf1 1 TTGAAATTCAACCGATCCCG GCAGCCGTTTCAGTATGGTA 20 20 45.0 50.0 56.4 58.0 4.0 0.0 1.598285 128917 129314 523-555 1.000000 74.2 rps15-ycf1_p2 rps15-ycf1 2 TTGAAATTCAACCGATCCCG AATGGCAGCCGTTTCAGTAT 20 20 45.0 45.0 56.4 57.9 4.0 13.7 1.708513 128917 129314 527-559 1.000000 73.3 rps15-ycf1_p3 rps15-ycf1 3 TTGAAATTCAACCGATCCCG ATAATGGCAGCCGTTTCAGT 20 20 45.0 45.0 56.4 57.9 4.0 24.3 1.708513 128917 129314 529-561 1.000000 73.3 rps15-ycf1_p4 rps15-ycf1 4 TTGAAATTCAACCGATCCCG CCAATAATGGCAGCCGTTTC 20 20 45.0 50.0 56.4 57.8 4.0 24.3 1.788068 128917 129314 532-564 1.000000 72.6 rps15-ycf1_p5 rps15-ycf1 5 TTTGAAATTCAACCGATCCCG GCAGCCGTTTCAGTATGGTA 21 20 42.9 50.0 57.2 58.0 8.1 0.0 1.859432 128917 129314 524-556 1.000000 72.1 trnN-GUU-trnR-ACG_p1 trnN-GUU-trnR-ACG 1 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 58.1 57.8 0.0 18.9 0.311620 135109 135806 762-906 1.000000 84.5 trnN-GUU-trnR-ACG_p2 trnN-GUU-trnR-ACG 2 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 57.8 57.8 15.9 18.9 0.366304 135109 135806 685-829 1.000000 84.3 trnN-GUU-trnR-ACG_p3 trnN-GUU-trnR-ACG 3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 58.2 57.8 18.9 18.9 0.372539 135109 135806 672-816 1.000000 84.3 trnN-GUU-trnR-ACG_p4 trnN-GUU-trnR-ACG 4 TGGGTTGAGAACGGGAATTG GAACAGATCCAGTGGAGACG 20 20 50.0 55.0 58.1 57.7 0.0 18.7 0.381540 135109 135806 799-943 1.000000 83.8 trnN-GUU-trnR-ACG_p5 trnN-GUU-trnR-ACG 5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 57.8 57.8 0.0 18.9 0.410108 135109 135806 761-905 1.000000 83.7