primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf80-trnR-CCG_p1	ycf80-trnR-CCG	1	AAATGTCTGTGTGGCTGTGT	CGGTCGAAGGTTCAAATCCT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.151900	7066	8032	1204-1204	0.333333	52.1
ycf80-trnR-CCG_p2	ycf80-trnR-CCG	2	AAATGTCTGTGTGGCTGTGT	TTGGGCATGTAACTCAGTGG	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.255964	7066	8032	1249-1249	0.333333	51.3
ycf80-trnR-CCG_p3	ycf80-trnR-CCG	3	AAATGTCTGTGTGGCTGTGT	AGTTCGTTCATTGCAGGAGT	20	20	45.0	45.0	57.9	57.4	0.0	0.0	0.607968	7066	8032	1900-1900	0.333333	48.5
ycf80-trnR-CCG_p4	ycf80-trnR-CCG	4	AAATGTCTGTGTGGCTGTGT	TGGGCATGTAACTCAGTGGA	20	20	45.0	50.0	57.9	58.6	0.0	0.0	0.700000	7066	8032	1248-1248	0.333333	47.7
ycf80-trnR-CCG_p5	ycf80-trnR-CCG	5	AAATGTCTGTGTGGCTGTGT	GGGCATGTAACTCAGTGGAT	20	20	45.0	50.0	57.9	57.3	0.0	0.0	0.774776	7066	8032	1247-1247	0.333333	47.1
cpeA-ccsA_p1	cpeA-ccsA	1	CGGCGGAAAAACTAGCTAGT	AATGGACGGCAGTTAATCCC	20	20	50.0	50.0	58.0	57.9	21.0	0.0	0.058753	12451	13020	1206-1206	0.333333	52.9
cpeA-ccsA_p2	cpeA-ccsA	2	CGGCGGAAAAACTAGCTAGT	CCCATACAGCTCCAGCAATT	20	20	50.0	50.0	58.0	57.9	21.0	0.5	0.068941	12451	13020	1671-1671	0.333333	52.8
cpeA-ccsA_p3	cpeA-ccsA	3	GCGCCTAATCAACTACTGCT	AATGGACGGCAGTTAATCCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.111457	12451	13020	1055-1055	0.333333	52.4
cpeA-ccsA_p4	cpeA-ccsA	4	GCGATAGAGTTTCTGCTGCT	AATGGACGGCAGTTAATCCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.119536	12451	13020	1470-1470	0.333333	52.4
cpeA-ccsA_p5	cpeA-ccsA	5	GCGCCTAATCAACTACTGCT	CCCATACAGCTCCAGCAATT	20	20	50.0	50.0	58.1	57.9	0.0	0.5	0.121646	12451	13020	1520-1520	0.333333	52.4
ycf22-psaC_p1	ycf22-psaC	1	AAACGGGGATCATCAAGACG	CCCTCAGAGGATAAAACGGC	20	20	50.0	55.0	58.0	58.0	0.0	19.2	0.069249	16256	16980	1561-1561	0.333333	52.8
ycf22-psaC_p2	ycf22-psaC	2	AAACGGGGATCATCAAGACG	GGACGTAACATAGGTGCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.088243	16256	16980	1801-1801	0.333333	52.6
ycf22-psaC_p3	ycf22-psaC	3	AAACGGGGATCATCAAGACG	GGTCAAGCAGGACGTAACAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.118938	16256	16980	1810-1810	0.333333	52.4
ycf22-psaC_p4	ycf22-psaC	4	AAACGGGGATCATCAAGACG	TGGAACGGATGTAAAGCTGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132126	16256	16980	1219-1219	0.333333	52.3
ycf22-psaC_p5	ycf22-psaC	5	AAACGGGGATCATCAAGACG	GGAACGGATGTAAAGCTGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.133600	16256	16980	1218-1218	0.333333	52.3
trnN-GUU-trnL-UAG_p1	trnN-GUU-trnL-UAG	1	CTACCACTGAGCTACTGAGGA	AGCGTGTCTACCAATTCCAC	21	20	52.4	50.0	58.0	57.9	11.2	0.0	1.100874	43456	43627	213-222	1.000000	82.5
trnN-GUU-trnL-UAG_p2	trnN-GUU-trnL-UAG	2	TCTACCACTGAGCTACTGAGG	AGCGTGTCTACCAATTCCAC	21	20	52.4	50.0	58.0	57.9	0.0	0.0	1.100874	43456	43627	214-223	1.000000	82.5
trnN-GUU-trnL-UAG_p3	trnN-GUU-trnL-UAG	3	CTACCACTGAGCTACTGAGGA	CTACCAATTCCACCACGTCC	21	20	52.4	55.0	58.0	58.3	11.2	0.0	1.271949	43456	43627	206-215	1.000000	81.3
trnN-GUU-trnL-UAG_p4	trnN-GUU-trnL-UAG	4	TCTACCACTGAGCTACTGAGG	CTACCAATTCCACCACGTCC	21	20	52.4	55.0	58.0	58.3	0.0	0.0	1.271949	43456	43627	207-216	1.000000	81.3
trnN-GUU-trnL-UAG_p5	trnN-GUU-trnL-UAG	5	ACCAATCGGTTAACAGCCG	AGCGTGTCTACCAATTCCAC	19	20	52.6	50.0	58.2	57.9	8.1	0.0	1.245681	43456	43627	238-247	1.000000	81.0
ycf53_p1	ycf53	1	AAATTCATTGACCAGCCCGA	GGTCCAGGAGATCCATCTGT	20	20	45.0	55.0	57.8	58.2	0.0	0.9	0.414443	52005	52724	1882-1883	1.000000	84.7
ycf53_p2	ycf53	2	AATTCATTGACCAGCCCGAA	GGTCCAGGAGATCCATCTGT	20	20	45.0	55.0	57.8	58.2	0.0	0.9	0.414443	52005	52724	1881-1882	1.000000	84.7
ycf53_p3	ycf53	3	AATTCATTGACCAGCCCGAA	TGGTCCAGGAGATCCATCTG	20	20	45.0	55.0	57.8	58.2	0.0	0.9	0.415750	52005	52724	1882-1883	1.000000	84.7
ycf53_p4	ycf53	4	TCCAAGTCGGCAATTCAGTC	GGTCCAGGAGATCCATCTGT	20	20	50.0	55.0	58.2	58.2	0.0	0.9	0.391656	52005	52724	1698-1698	0.333333	50.2
ycf53_p5	ycf53	5	TCCAAGTCGGCAATTCAGTC	TGGTCCAGGAGATCCATCTG	20	20	50.0	55.0	58.2	58.2	0.0	0.9	0.392963	52005	52724	1699-1699	0.333333	50.2
rrn16-ompR_p1	rrn16-ompR	1	CATTGCGGAAAATTCCCCAC	TGCTCCTAGTTCTAGTCCGG	20	20	50.0	55.0	58.0	57.9	6.6	0.0	0.062984	67365	69148	2344-2344	0.333333	52.8
rrn16-ompR_p2	rrn16-ompR	2	CATTGCGGAAAATTCCCCAC	GCTCCTAGTTCTAGTCCGGT	20	20	50.0	55.0	58.0	57.9	6.6	0.0	0.064436	67365	69148	2343-2343	0.333333	52.8
rrn16-ompR_p3	rrn16-ompR	3	AGCTACTGATCGTTGCCTTG	TGCTCCTAGTTCTAGTCCGG	20	20	50.0	55.0	58.0	57.9	15.8	0.0	0.069968	67365	69148	2259-2259	0.333333	52.8
rrn16-ompR_p4	rrn16-ompR	4	CAGCTACTGATCGTTGCCTT	TGCTCCTAGTTCTAGTCCGG	20	20	50.0	55.0	58.0	57.9	15.8	0.0	0.069968	67365	69148	2260-2260	0.333333	52.8
rrn16-ompR_p5	rrn16-ompR	5	AGCTACTGATCGTTGCCTTG	GCTCCTAGTTCTAGTCCGGT	20	20	50.0	55.0	58.0	57.9	15.8	0.0	0.071420	67365	69148	2258-2258	0.333333	52.8
ompR-psbD_p1	ompR-psbD	1	CTCAATAGGAAGAGGCTTGCA	TGCTTGCCATGAATACAGCT	21	20	47.6	45.0	57.7	57.9	0.0	0.0	1.405855	70864	71113	1344-1344	0.333333	42.1
ompR-psbD_p2	ompR-psbD	2	CACTGTACCTATCATTCGCGA	TGCTTGCCATGAATACAGCT	21	20	47.6	45.0	57.7	57.9	3.7	0.0	1.482770	70864	71113	813-813	0.333333	41.5
ompR-psbD_p3	ompR-psbD	3	TCACTGTACCTATCATTCGCG	TGCTTGCCATGAATACAGCT	21	20	47.6	45.0	57.7	57.9	0.0	0.0	1.482770	70864	71113	814-814	0.333333	41.5
ompR-psbD_p4	ompR-psbD	4	CTCAATAGGAAGAGGCTTGCA	CCGCTCATCCGGTATTTCTC	21	20	47.6	55.0	57.7	58.2	0.0	0.0	1.488049	70864	71113	1160-1160	0.333333	41.4
ompR-psbD_p5	ompR-psbD	5	CACTGTACCTATCATTCGCGA	CCGCTCATCCGGTATTTCTC	21	20	47.6	55.0	57.7	58.2	3.7	0.0	1.564963	70864	71113	629-629	0.333333	40.8
syh_p1	syh	1	TCCTTGCCCGATCACAAAAA	ATACTGGGGTGGGAGGATTC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.190619	80146	81402	1461-1461	0.333333	51.8
syh_p2	syh	2	TCCTTGCCCGATCACAAAAA	TGGCTACACCCCAATAGCTA	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.195795	80146	81402	1399-1399	0.333333	51.8
syh_p3	syh	3	TCCTTGCCCGATCACAAAAA	CTTGGCTACACCCCAATAGC	20	20	45.0	55.0	58.0	58.3	0.0	0.0	0.331369	80146	81402	1401-1401	0.333333	50.7
syh_p4	syh	4	TCCTTGCCCGATCACAAAAA	GCTTGGCTACACCCCAATAG	20	20	45.0	55.0	58.0	58.3	0.0	0.0	0.331369	80146	81402	1402-1402	0.333333	50.7
syh_p5	syh	5	TGCCATTGGATTAGCACCAG	ATACTGGGGTGGGAGGATTC	20	20	50.0	55.0	58.2	58.2	6.9	0.0	0.412418	80146	81402	2031-2031	0.333333	50.0
rpl11-trnW-CCA_p1	rpl11-trnW-CCA	1	GCGAGCTGTTCCAGATACAA	TGCAGAAGACGCTAATCCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020290	90426	91766	2058-2834	0.666667	78.5
rpl11-trnW-CCA_p2	rpl11-trnW-CCA	2	GCGAGCTGTTCCAGATACAA	GATGTCGAGGGTTCAAGTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.021685	90426	91766	1771-2560	0.666667	78.5
rpl11-trnW-CCA_p3	rpl11-trnW-CCA	3	AGGCTGTCCTGAACCTTTTG	TGCAGAAGACGCTAATCCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023263	90426	91766	1935-1935	0.333333	53.1
rpl11-trnW-CCA_p4	rpl11-trnW-CCA	4	AGGCTGTCCTGAACCTTTTG	GATGTCGAGGGTTCAAGTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.024658	90426	91766	1648-1648	0.333333	53.1
rpl11-trnW-CCA_p5	rpl11-trnW-CCA	5	CGTGTTAAGATCCTGCAGCT	TGCAGAAGACGCTAATCCAC	20	20	50.0	50.0	58.0	58.0	8.7	0.0	0.025881	90426	91766	2013-2013	0.333333	53.1
moeB-rpl9_p1	moeB-rpl9	1	ACCGGGAATAATTGGCGTTT	CTTGCCTTCTTGACCCAGTT	20	20	45.0	50.0	58.2	58.0	6.3	0.0	0.171066	93063	94833	2363-2363	0.333333	52.0
moeB-rpl9_p2	moeB-rpl9	2	AAGGTGGTCCGAGGTATTCT	CTTGCCTTCTTGACCCAGTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.262427	93063	94833	2451-2451	0.333333	51.2
moeB-rpl9_p3	moeB-rpl9	3	ACCGGGAATAATTGGCGTTT	TTCTTGACCCAGTTTTGGCA	20	20	45.0	45.0	58.2	57.8	6.3	0.0	0.309451	93063	94833	2357-2357	0.333333	50.9
moeB-rpl9_p4	moeB-rpl9	4	GCAGCTCTAATTCTCGTGCA	CTTGCCTTCTTGACCCAGTT	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.368084	93063	94833	1980-1980	0.333333	50.4
moeB-rpl9_p5	moeB-rpl9	5	TGCAGCTCTAATTCTCGTGC	CTTGCCTTCTTGACCCAGTT	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.368084	93063	94833	1981-1981	0.333333	50.4
clpC-rpl19_p1	clpC-rpl19	1	CCCCAGTTGTCTTGCTTCTT	CCAAGAAGACGCTCTACCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.015449	100053	101083	2046-2046	0.333333	53.2
clpC-rpl19_p2	clpC-rpl19	2	AGAAGGCCTACATCTAGCGT	CCAAGAAGACGCTCTACCAC	20	20	50.0	55.0	57.9	58.0	5.2	0.0	0.077059	100053	101083	2495-2495	0.333333	52.7
clpC-rpl19_p3	clpC-rpl19	3	CCCCAGTTGTCTTGCTTCTT	ACCGACCCATAAGGATGCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.110228	100053	101083	1927-1927	0.333333	52.5
clpC-rpl19_p4	clpC-rpl19	4	CCCCAGTTGTCTTGCTTCTT	AGACGCTCTACCACTAAGCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.144893	100053	101083	2040-2040	0.333333	52.2
clpC-rpl19_p5	clpC-rpl19	5	CCCCAGTTGTCTTGCTTCTT	TTACTAATTGGGCCGGGTTG	20	20	50.0	50.0	58.0	57.9	0.0	2.2	0.144893	100053	101083	2009-2009	0.333333	52.2
ycf38_p1	ycf38	1	CAACAGTGCCAGCTGCTATA	GCTCCTGCACGACAAGAATA	20	20	50.0	50.0	58.0	58.0	8.7	0.0	0.038565	110242	111117	1942-1942	0.666667	73.7
ycf38_p2	ycf38	2	CAGAAATTAAGCGTCCGGGA	GCTCCTGCACGACAAGAATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028687	110242	111117	1381-1381	0.333333	53.1
ycf38_p3	ycf38	3	GCTAGTATCCCAACAGTGCC	GCTCCTGCACGACAAGAATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.055218	110242	111117	1952-1952	0.333333	52.9
ycf38_p4	ycf38	4	ATGGGTTAAATCCGTCTGGC	GCTCCTGCACGACAAGAATA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.062927	110242	111117	1894-1894	0.333333	52.8
ycf38_p5	ycf38	5	CAGAAATTAAGCGTCCGGGA	AACTTGAACAAGCTCCTGCA	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.075679	110242	111117	1392-1392	0.333333	52.7
rps5_p1	rps5	1	GACTCCATTTTGCCCAAAGC	CAAGGTAGCGAGACTGTTCC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.086940	118832	119359	1099-1099	0.333333	52.6
rps5_p2	rps5	2	CGCCAACCCTATCTTTGTGA	CAAGGTAGCGAGACTGTTCC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.110281	118832	119359	933-933	0.333333	52.5
rps5_p3	rps5	3	GACTCCATTTTGCCCAAAGC	AAGGTAGCGAGACTGTTCCT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.158501	118832	119359	1098-1098	0.333333	52.1
rps5_p4	rps5	4	CGCCAACCCTATCTTTGTGA	AAGGTAGCGAGACTGTTCCT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.181842	118832	119359	932-932	0.333333	51.9
rps5_p5	rps5	5	GAAGCGCCATTACCTATCCC	CAAGGTAGCGAGACTGTTCC	20	20	55.0	55.0	58.2	58.0	2.3	0.0	0.192647	118832	119359	1467-1467	0.333333	51.8
atpB_p1	atpB	1	GTTGCTTCAGCGAGTTCAAC	ATTATGCCGCGCTTACAGAA	20	20	50.0	45.0	58.0	58.1	0.0	5.6	0.076071	154086	155507	2507-2507	0.333333	52.7
atpB_p2	atpB	2	GTTGTTGCTTCAGCGAGTTC	ATTATGCCGCGCTTACAGAA	20	20	50.0	45.0	58.0	58.1	0.0	5.6	0.076071	154086	155507	2510-2510	0.333333	52.7
atpB_p3	atpB	3	TTGCTTCAGCGAGTTCAACT	ATTATGCCGCGCTTACAGAA	20	20	45.0	45.0	58.0	58.1	0.0	5.6	0.098337	154086	155507	2506-2506	0.333333	52.5
atpB_p4	atpB	4	GTTGCTTCAGCGAGTTCAAC	TGTCAGCTCAATCAGAAGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129182	154086	155507	2377-2377	0.333333	52.3
atpB_p5	atpB	5	GTTGTTGCTTCAGCGAGTTC	TGTCAGCTCAATCAGAAGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129182	154086	155507	2380-2380	0.333333	52.3
orf12-trnG-UCC_p1	orf12-trnG-UCC	1	ATCCTGAAGTTTTTGGGCTT	ACACGTGTTGGACAACCTTT	20	20	40.0	45.0	55.4	58.2	0.0	17.2	2.769693	185681	186330	1419-1419	0.333333	31.2
orf12-trnG-UCC_p2	orf12-trnG-UCC	2	ATCCTGAAGTTTTTGGGCTT	AACCAGGCAACATCACTAGC	20	20	40.0	50.0	55.4	58.2	0.0	0.0	2.772287	185681	186330	1380-1380	0.333333	31.2
orf12-trnG-UCC_p3	orf12-trnG-UCC	3	ATCCTGAAGTTTTTGGGCTT	TTCCAAGCTAATGATGCGGG	20	20	40.0	50.0	55.4	58.3	0.0	0.0	2.919533	185681	186330	1206-1206	0.333333	30.0
orf12-trnG-UCC_p4	orf12-trnG-UCC	4	ATCCTGAAGTTTTTGGGCTT	CCAGGCAACATCACTAGCTC	20	20	40.0	55.0	55.4	58.3	0.0	0.0	2.937331	185681	186330	1378-1378	0.333333	29.8
orf12-trnG-UCC_p5	orf12-trnG-UCC	5	ATCCTGAAGTTTTTGGGCTT	CAGGCAACATCACTAGCTCA	20	20	40.0	50.0	55.4	57.6	0.0	0.0	2.990288	185681	186330	1377-1377	0.333333	29.4
trnG-UCC-pafII_p1	trnG-UCC-pafII	1	CCCGCATCATTAGCTTGGAA	AACCAGGCAACATCACTAGC	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.494476	186402	186481	192-194	1.000000	84.9
trnG-UCC-pafII_p2	trnG-UCC-pafII	2	CCCGCATCATTAGCTTGGAA	CCAGGCAACATCACTAGCTC	20	20	50.0	55.0	58.3	58.3	0.0	0.0	0.659520	186402	186481	190-192	1.000000	83.6
trnG-UCC-pafII_p3	trnG-UCC-pafII	3	ATCGAACCCGCATCATTAGC	AACCAGGCAACATCACTAGC	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.660689	186402	186481	198-200	1.000000	83.5
trnG-UCC-pafII_p4	trnG-UCC-pafII	4	CCCGCATCATTAGCTTGGAA	CAGGCAACATCACTAGCTCA	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.712477	186402	186481	189-191	1.000000	83.1
trnG-UCC-pafII_p5	trnG-UCC-pafII	5	AACCCGCATCATTAGCTTGG	AACCAGGCAACATCACTAGC	20	20	50.0	50.0	58.6	58.2	0.0	0.0	0.781026	186402	186481	194-196	1.000000	82.6