primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rns-trnI(gat)_p1 rns-trnI(gat) 1 GGGGTGAAGTCGTAACAAGG GGTGGGCTATCCTGGATTTG 20 20 55.0 55.0 58.2 58.0 0.0 15.5 0.208517 1481 1583 227-236 1.000000 88.3 rns-trnI(gat)_p2 rns-trnI(gat) 2 CAAGGTAGCCGTACTGGAAG GGTGGGCTATCCTGGATTTG 20 20 55.0 55.0 57.7 58.0 7.8 15.5 0.308371 1481 1583 212-221 1.000000 88.2 rns-trnI(gat)_p3 rns-trnI(gat) 3 GGGGTGAAGTCGTAACAAGG GGATTTGAACCAGGGACCTC 20 20 55.0 55.0 58.2 57.9 0.0 0.0 0.325671 1481 1583 214-223 1.000000 87.9 rns-trnI(gat)_p4 rns-trnI(gat) 4 AGTAACTGGGGTGAAGTCGT GGTGGGCTATCCTGGATTTG 20 20 50.0 55.0 58.3 58.0 0.0 15.5 0.299820 1481 1583 234-243 1.000000 87.3 rns-trnI(gat)_p5 rns-trnI(gat) 5 GGGGTGAAGTCGTAACAAGG ATGGTGGGCTATCCTGGATT 20 20 55.0 50.0 58.2 58.2 0.0 15.5 0.356788 1481 1583 229-238 1.000000 87.0 rrn5-rps6_p1 rrn5-rps6 1 ATCTCCCCCAAGAGTTCACA GACCGCCCATTCATAAGGTT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.097575 4896 5535 1841-1841 0.333333 52.6 rrn5-rps6_p2 rrn5-rps6 2 TCTCCCCCAAGAGTTCACAT GACCGCCCATTCATAAGGTT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.097575 4896 5535 1840-1840 0.333333 52.6 rrn5-rps6_p3 rrn5-rps6 3 CAAGTCGAGCAGAGACGAAA GACCGCCCATTCATAAGGTT 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.104661 4896 5535 1941-1941 0.333333 52.5 rrn5-rps6_p4 rrn5-rps6 4 TTCGGTCCATATCCGGTGTA GACCGCCCATTCATAAGGTT 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.123087 4896 5535 1696-1696 0.333333 52.3 rrn5-rps6_p5 rrn5-rps6 5 CCTTAGTGATCCGACAGTGC GACCGCCCATTCATAAGGTT 20 20 55.0 50.0 58.1 57.9 0.0 0.0 0.124887 4896 5535 1916-1916 0.333333 52.3 thiG_p1 thiG 1 AAGCACGAAAAGTTGCTCCT AACGAACAGCAGGAAAAGGA 20 20 45.0 45.0 58.3 57.7 0.0 0.0 0.661509 6645 7475 1854-1858 0.666667 63.6 thiG_p2 thiG 2 AACCTTATGAATGGGCGGTC AACGAACAGCAGGAAAAGGA 20 20 50.0 45.0 57.9 57.7 0.0 0.0 0.394629 6645 7475 1793-1793 0.333333 50.2 thiG_p3 thiG 3 TATGAATGGGCGGTCAGGTA AACGAACAGCAGGAAAAGGA 20 20 50.0 45.0 58.2 57.7 0.0 0.0 0.545830 6645 7475 1788-1788 0.333333 49.0 thiG_p4 thiG 4 TGAATGGGCGGTCAGGTATA AACGAACAGCAGGAAAAGGA 20 20 50.0 45.0 58.2 57.7 0.0 0.0 0.545830 6645 7475 1786-1786 0.333333 49.0 thiG_p5 thiG 5 AACCTTATGAATGGGCGGTC ACGAACAGCAGGAAAAGGAT 20 20 50.0 45.0 57.9 57.4 0.0 0.0 0.621405 6645 7475 1792-1792 0.333333 48.4 trnN(gtt)-trnL(tag)_p1 trnN(gtt)-trnL(tag) 1 CTCTGCCACTGAGCTACTGA AGCGTGTCTACCAATTCCAC 20 20 55.0 50.0 58.8 57.9 0.0 0.0 0.913855 10379 10533 205-208 1.000000 83.0 trnN(gtt)-trnL(tag)_p2 trnN(gtt)-trnL(tag) 2 TCTGCCACTGAGCTACTGAG AGCGTGTCTACCAATTCCAC 20 20 55.0 50.0 58.8 57.9 0.0 0.0 0.913855 10379 10533 204-207 1.000000 83.0 trnN(gtt)-trnL(tag)_p3 trnN(gtt)-trnL(tag) 3 CTCTGCCACTGAGCTACTGA TACCAATTCCACCATGTCCG 20 20 55.0 50.0 58.8 57.6 0.0 0.0 1.230721 10379 10533 197-200 1.000000 80.6 trnN(gtt)-trnL(tag)_p4 trnN(gtt)-trnL(tag) 4 TCTGCCACTGAGCTACTGAG TACCAATTCCACCATGTCCG 20 20 55.0 50.0 58.8 57.6 0.0 0.0 1.230721 10379 10533 196-199 1.000000 80.6 trnN(gtt)-trnL(tag)_p5 trnN(gtt)-trnL(tag) 5 ACCAATCGGTTAACAGCCG AGCGTGTCTACCAATTCCAC 19 20 52.6 50.0 58.2 57.9 8.1 0.0 1.245681 10379 10533 228-231 1.000000 80.1 gltB-psaC_p1 gltB-psaC 1 GGGAGGGATTGTCGTTGTTT GTTTCACAGCGCTTACAACC 20 20 50.0 50.0 58.1 58.0 0.0 7.5 0.126777 49886 50417 1036-1036 0.333333 52.3 gltB-psaC_p2 gltB-psaC 2 ACGGATAGAACTGTTGGTGC GTTTCACAGCGCTTACAACC 20 20 50.0 50.0 57.9 58.0 0.0 7.5 0.127176 49886 50417 1428-1428 0.333333 52.3 gltB-psaC_p3 gltB-psaC 3 TGGTGCTAGGATATCGGGTT GTTTCACAGCGCTTACAACC 20 20 50.0 50.0 57.9 58.0 0.0 7.5 0.129524 49886 50417 1414-1414 0.333333 52.3 gltB-psaC_p4 gltB-psaC 4 TTGGTGCTAGGATATCGGGT GTTTCACAGCGCTTACAACC 20 20 50.0 50.0 57.9 58.0 0.0 7.5 0.129524 49886 50417 1415-1415 0.333333 52.3 gltB-psaC_p5 gltB-psaC 5 GGGAGGGATTGTCGTTGTTT TTTCACAGCGCTTACAACCT 20 20 50.0 45.0 58.1 58.0 0.0 7.5 0.132569 49886 50417 1035-1035 0.333333 52.3 ycf23_p1 ycf23 1 GGTTGTAAGCGCTGTGAAAC GCTGAGAAATTCTTGTAGCAGC 20 22 50.0 45.5 58.0 58.0 12.6 14.8 2.045996 50816 51616 1334-1602 0.666667 62.3 ycf23_p2 ycf23 2 AGGTTGTAAGCGCTGTGAAA GCTGAGAAATTCTTGTAGCAGC 20 22 45.0 45.5 58.0 58.0 12.6 14.8 2.051789 50816 51616 1335-1603 0.666667 62.3 ycf23_p3 ycf23 3 GGTTGTAAGCGCTGTGAAAC CGTCAAAACGCTGAGAAATTCT 20 22 50.0 40.9 58.0 57.9 12.6 0.0 2.166271 50816 51616 1611-1611 0.333333 36.0 ycf23_p4 ycf23 4 AGGTTGTAAGCGCTGTGAAA CGTCAAAACGCTGAGAAATTCT 20 22 45.0 40.9 58.0 57.9 12.6 0.0 2.172063 50816 51616 1612-1612 0.333333 36.0 ycf23_p5 ycf23 5 CTCAATGTGTACGTGCATGC GCTGAGAAATTCTTGTAGCAGC 20 22 50.0 45.5 57.8 58.0 11.8 14.8 2.177408 50816 51616 1706-1706 0.333333 35.9 ycf91_p1 ycf91 1 CCACCTGGTCTTAGAAGCTC CCCTCGACTTTCAGAATCGG 20 20 55.0 55.0 57.3 58.1 0.0 6.4 0.754260 58521 58778 874-874 0.333333 47.3 ycf91_p2 ycf91 2 CCACCTGGTCTTAGAAGCTC GAAGGACTTGAACCCTCGAC 20 20 55.0 55.0 57.3 57.9 0.0 0.0 0.763881 58521 58778 886-886 0.333333 47.2 ycf91_p3 ycf91 3 CCACCTGGTCTTAGAAGCTC AGAAACCTAGCAGTCCCGAT 20 20 55.0 50.0 57.3 58.1 0.0 0.0 0.814792 58521 58778 1166-1166 0.333333 46.8 ycf91_p4 ycf91 4 CCACCTGGTCTTAGAAGCTC ACCTAGCAGTCCCGATAACA 20 20 55.0 50.0 57.3 57.8 0.0 0.0 0.836722 58521 58778 1162-1162 0.333333 46.6 ycf91_p5 ycf91 5 CCACCTGGTCTTAGAAGCTC AGCATGGAAGGACTTGAACC 20 20 55.0 50.0 57.3 57.8 0.0 0.0 0.888732 58521 58778 892-892 0.333333 46.2 trnR(ccg)-orf441_p1 trnR(ccg)-orf441 1 CCGATTCTGAAAGTCGAGGG TCTCTATCCTGCCAGCAAGT 20 20 55.0 50.0 58.1 58.1 13.4 0.0 0.200687 59071 59876 1001-1126 0.666667 71.2 trnR(ccg)-orf441_p2 trnR(ccg)-orf441 2 GTCGAGGGTTCAAGTCCTTC TCTCTATCCTGCCAGCAAGT 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.210308 59071 59876 989-1114 0.666667 71.1 trnR(ccg)-orf441_p3 trnR(ccg)-orf441 3 CCGATTCTGAAAGTCGAGGG GTTGTCTCTATCCTGCCAGC 20 20 55.0 55.0 58.1 58.1 13.4 0.0 0.125360 59071 59876 1130-1130 0.333333 52.3 trnR(ccg)-orf441_p4 trnR(ccg)-orf441 4 GTCGAGGGTTCAAGTCCTTC GTTGTCTCTATCCTGCCAGC 20 20 55.0 55.0 57.9 58.1 0.0 0.0 0.134980 59071 59876 1118-1118 0.333333 52.3 trnR(ccg)-orf441_p5 trnR(ccg)-orf441 5 GGTTCAAGTCCTTCCATGCT GTTGTCTCTATCCTGCCAGC 20 20 50.0 55.0 57.8 58.1 0.0 0.0 0.259831 59071 59876 1112-1112 0.333333 51.3 trnG(tcc)-trnM_p1 trnG(tcc)-trnM 1 TTCCAAGCTAATGATGCGGG GGTTCGAATCCAGCCAGATG 20 20 50.0 55.0 58.3 58.7 0.0 0.0 1.017552 69729 69783 113-117 1.000000 82.2 trnG(tcc)-trnM_p2 trnG(tcc)-trnM 2 GCTAATGATGCGGGTTCGAT GGTTCGAATCCAGCCAGATG 20 20 50.0 55.0 58.5 58.7 0.0 0.0 1.183765 69729 69783 107-111 1.000000 81.5 trnG(tcc)-trnM_p3 trnG(tcc)-trnM 3 CCAAGCTAATGATGCGGGTT GGTTCGAATCCAGCCAGATG 20 20 50.0 55.0 58.6 58.7 0.0 0.0 1.304101 69729 69783 111-115 1.000000 80.1 trnG(tcc)-trnM_p4 trnG(tcc)-trnM 4 AATGATGCGGGTTCGATTCC GGTTCGAATCCAGCCAGATG 20 20 50.0 55.0 58.7 58.7 0.0 0.0 1.387693 69729 69783 104-108 1.000000 80.1 trnG(tcc)-trnM_p5 trnG(tcc)-trnM 5 AAGCTAATGATGCGGGTTCG GGTTCGAATCCAGCCAGATG 20 20 50.0 55.0 58.7 58.7 0.0 0.0 1.397150 69729 69783 109-113 1.000000 79.6 trnM_p1 trnM 1 TTCGCTTTGGTTTTCCTGGT GGATTGCCAGAAAGTCGAGA 20 20 45.0 50.0 58.2 57.6 0.0 0.0 0.616366 69784 71906 2738-2740 0.666667 65.6 trnM_p2 trnM 2 TTCGCTTTGGTTTTCCTGGT AGGATTGCCAGAAAGTCGAG 20 20 45.0 50.0 58.2 57.6 0.0 0.0 0.622977 69784 71906 2739-2741 0.666667 65.5 trnM_p3 trnM 3 TTCCAAGCTAATGATGCGGG GGATTGCCAGAAAGTCGAGA 20 20 50.0 50.0 58.3 57.6 0.0 0.0 0.707522 69784 71906 2408-2410 0.666667 65.0 trnM_p4 trnM 4 TTCCAAGCTAATGATGCGGG AGGATTGCCAGAAAGTCGAG 20 20 50.0 50.0 58.3 57.6 0.0 0.0 0.714132 69784 71906 2409-2411 0.666667 64.9 trnM_p5 trnM 5 CCGCTACCCGCTTCAATAAA GGATTGCCAGAAAGTCGAGA 20 20 50.0 50.0 58.3 57.6 0.0 0.0 0.727667 69784 71906 2380-2382 0.666667 64.9 orf461-trnM_p1 orf461-trnM 1 CCAAGGCAAATTTTTCCACTGA GGATTGCCAGAAAGTCGAGA 22 20 40.9 50.0 57.9 57.6 8.5 0.0 2.457290 71288 71869 860-862 0.666667 50.1 orf461-trnM_p2 orf461-trnM 2 CCAAGGCAAATTTTTCCACTGA TTCTTAAGCATCTGTGGCCG 22 20 40.9 50.0 57.9 58.3 8.5 0.0 2.332791 71288 71869 677-677 0.333333 34.7 orf461-trnM_p3 orf461-trnM 3 TCCAAGGCAAATTTTTCCACTG TTCTTAAGCATCTGTGGCCG 22 20 40.9 50.0 57.9 58.3 8.5 0.0 2.332791 71288 71869 678-678 0.333333 34.7 orf461-trnM_p4 orf461-trnM 4 ACTCCAAGGCAAATTTTTCCAC TTCTTAAGCATCTGTGGCCG 22 20 40.9 50.0 57.9 58.3 8.5 0.0 2.334122 71288 71869 680-680 0.333333 34.7 orf461-trnM_p5 orf461-trnM 5 TCTAGCATTCCACTCAGATCCT TTCTTAAGCATCTGTGGCCG 22 20 45.5 50.0 58.1 58.3 0.0 0.0 2.341058 71288 71869 1016-1016 0.333333 34.6 orf445_p1 orf445 1 TCAAGGCTATCAATGGACTAACA CCATCACGAAACCCTAACAGA 23 21 39.1 47.6 57.3 57.7 0.0 0.0 5.020497 73620 74957 2068-2068 0.333333 13.3 orf445_p2 orf445 2 TCAAGGCTATCAATGGACTAACA TCCATCACGAAACCCTAACAG 23 21 39.1 47.6 57.3 57.7 0.0 0.0 5.020497 73620 74957 2069-2069 0.333333 13.3 orf445_p3 orf445 3 TCAAGGCTATCAATGGACTAACA ATGATCCATCACGAAACCCT 23 20 39.1 45.0 57.3 56.6 0.0 0.0 5.096569 73620 74957 2073-2073 0.333333 13.3 orf445_p4 orf445 4 TCAAGGCTATCAATGGACTAACA TCCATCACGAAACCCTAACA 23 20 39.1 45.0 57.3 56.5 0.0 0.0 5.219223 73620 74957 2069-2069 0.333333 13.3 orf445_p5 orf445 5 TCAAGGCTATCAATGGACTAACA AATGATCCATCACGAAACCCT 23 21 39.1 42.9 57.3 57.3 0.0 0.0 5.344172 73620 74957 2074-2074 0.333333 13.3 preA-rpl20_p1 preA-rpl20 1 TGGAAAACCAGCAGGATCAG AATGCTGCTGTTCGTCCTTA 20 20 50.0 45.0 57.8 57.5 0.3 0.0 0.677718 111611 111996 789-837 1.000000 86.6 preA-rpl20_p2 preA-rpl20 2 TGGAAAACCAGCAGGATCAG TGCTGCTGTTCGTCCTTATG 20 20 50.0 50.0 57.8 58.3 0.3 0.0 0.479382 111611 111996 832-835 0.666667 69.2 preA-rpl20_p3 preA-rpl20 3 CTCTTGGAAAACCAGCAGGA TGCTGCTGTTCGTCCTTATG 20 20 50.0 50.0 57.7 58.3 0.3 0.0 0.551092 111611 111996 839-839 0.333333 48.9 preA-rpl20_p4 preA-rpl20 4 TCTCTTGGAAAACCAGCAGG TGCTGCTGTTCGTCCTTATG 20 20 50.0 50.0 57.7 58.3 0.0 0.0 0.551092 111611 111996 840-840 0.333333 48.9 preA-rpl20_p5 preA-rpl20 5 CGCCGCAATGTTAAGTGAAA TGCTGCTGTTCGTCCTTATG 20 20 45.0 50.0 57.7 58.3 0.0 0.0 0.598299 111611 111996 961-961 0.333333 48.5 acpP_p1 acpP 1 GGAGAGGTGGTAGAGTTGGT TCGTAGTTGTGGTGAAGAGC 20 20 55.0 50.0 58.1 57.6 0.0 0.0 0.493888 117634 117897 536-564 1.000000 88.0 acpP_p2 acpP 2 CATCTCCAACTTTCGGTGGT TCGTAGTTGTGGTGAAGAGC 20 20 50.0 50.0 57.8 57.6 0.0 0.0 0.618873 117634 117897 1054-1081 0.666667 68.0 acpP_p3 acpP 3 CAGAAGTGGTGTCGGCAATA TCGTAGTTGTGGTGAAGAGC 20 20 50.0 50.0 57.9 57.6 0.0 0.0 0.527175 117634 117897 774-774 0.333333 49.1 acpP_p4 acpP 4 AACTTTCGGTGGTAGCACAG TCGTAGTTGTGGTGAAGAGC 20 20 50.0 50.0 58.1 57.6 25.2 0.0 0.554915 117634 117897 1074-1074 0.333333 48.9 acpP_p5 acpP 5 GAATCCCACCCTCTCCTCAT TCGTAGTTGTGGTGAAGAGC 20 20 55.0 50.0 58.2 57.6 0.0 0.0 0.620766 117634 117897 495-495 0.333333 48.4 petF_p1 petF 1 GCTGCAGAAATGGTAATGCC ACCGGAATTTATTGCTCCTGT 20 21 50.0 42.9 57.8 57.6 20.4 9.5 1.580439 143979 144275 1431-1438 0.666667 59.0 petF_p2 petF 2 GGATCAGCTGCAGAAATGGT ACCGGAATTTATTGCTCCTGT 20 21 50.0 42.9 58.2 57.6 20.4 9.5 1.605860 143979 144275 1437-1444 0.666667 58.8 petF_p3 petF 3 GGATGATGGATCAGCTGCAG ACCGGAATTTATTGCTCCTGT 20 21 55.0 42.9 58.5 57.6 10.1 9.5 1.841203 143979 144275 1444-1451 0.666667 56.9 petF_p4 petF 4 TGGTAATGCCTGGAGATCGT ACCGGAATTTATTGCTCCTGT 20 21 50.0 42.9 58.5 57.6 0.0 9.5 1.869992 143979 144275 1421-1428 0.666667 56.6 petF_p5 petF 5 TGGATCAGCTGCAGAAATGG ACCGGAATTTATTGCTCCTGT 20 21 50.0 42.9 58.2 57.6 20.4 9.5 1.607116 143979 144275 1445-1445 0.333333 40.5 ycf38_p1 ycf38 1 CAACCTCTTCAGCTGCATCT TGGAAAGCACCGAATCCAAA 20 20 50.0 45.0 57.9 58.0 9.5 0.0 0.122608 144514 145410 1819-1819 0.333333 52.4 ycf38_p2 ycf38 2 CAACCTCTTCAGCTGCATCT AGAGCTGGTTCTCCTGTTCT 20 20 50.0 50.0 57.9 58.0 9.5 0.0 0.133619 144514 145410 1747-1747 0.333333 52.3 ycf38_p3 ycf38 3 CAACCTCTTCAGCTGCATCT TGTTACCCCTAGTCTTGCCA 20 20 50.0 50.0 57.9 58.0 9.5 0.0 0.143866 144514 145410 1565-1565 0.333333 52.2 ycf38_p4 ycf38 4 ACCTTGTCCTATCTGCTCGT TGGAAAGCACCGAATCCAAA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.157744 144514 145410 1941-1941 0.333333 52.1 ycf38_p5 ycf38 5 CAACCTCTTCAGCTGCATCT TACTCCAACCTCCCCAAGAG 20 20 50.0 55.0 57.9 58.0 9.5 0.0 0.158280 144514 145410 1587-1587 0.333333 52.1 rpl11-trnW(cca)_p1 rpl11-trnW(cca) 1 CTACTGGAGGAGCTGGAGTT GTCGAGGGTTCAAGTCCTTC 20 20 55.0 55.0 58.1 57.9 0.0 0.0 0.222423 168460 168709 337-351 1.000000 89.9 rpl11-trnW(cca)_p2 rpl11-trnW(cca) 2 TTTGGTTGTCCTGATCCAGC GTCGAGGGTTCAAGTCCTTC 20 20 50.0 55.0 58.1 57.9 0.0 0.0 0.172026 168460 168709 542-556 1.000000 88.9 rpl11-trnW(cca)_p3 rpl11-trnW(cca) 3 TTTGGTTGTCCTGATCCAGC GGCAGAACGTAGGTTTCCAA 20 20 50.0 50.0 58.1 58.1 0.0 1.8 0.203414 168460 168709 570-584 1.000000 88.6 rpl11-trnW(cca)_p4 rpl11-trnW(cca) 4 TTTGGTTGTCCTGATCCAGC TTGGCAGAACGTAGGTTTCC 20 20 50.0 50.0 58.1 58.1 0.0 1.8 0.203414 168460 168709 572-586 1.000000 88.6 rpl11-trnW(cca)_p5 rpl11-trnW(cca) 5 TTTGGTTGTCCTGATCCAGC CAGTTGGCAGAACGTAGGTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.211347 168460 168709 575-589 1.000000 88.5 psbD-ompR_p1 psbD-ompR 1 GCACATAACAATGCACCACC AGATGGTTATGGCGTGTGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.029260 178707 179229 1699-1699 0.333333 53.1 psbD-ompR_p2 psbD-ompR 2 GGCACATAACAATGCACCAC AGATGGTTATGGCGTGTGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.029260 178707 179229 1700-1700 0.333333 53.1 psbD-ompR_p3 psbD-ompR 3 CAATCCAGACCAACCCACAA AGATGGTTATGGCGTGTGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.030220 178707 179229 1169-1169 0.333333 53.1 psbD-ompR_p4 psbD-ompR 4 ACAAAAACAGCAATTGGCCC AGATGGTTATGGCGTGTGTC 20 20 45.0 50.0 58.0 58.0 7.7 0.0 0.058241 178707 179229 1525-1525 0.333333 52.9 psbD-ompR_p5 psbD-ompR 5 TCTGCTGCATCACCATCTTC AGATGGTTATGGCGTGTGTC 20 20 50.0 50.0 58.0 58.0 1.3 0.0 0.058280 178707 179229 1753-1753 0.333333 52.9