primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	GTCACAAAGGTTGTCCCTGT	CGCCAGGAATGAGAAACGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.057365	4012	4595	954-956	1.000000	82.1
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	GTCACAAAGGTTGTCCCTGT	TACGCCAGGAATGAGAAACG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.057365	4012	4595	956-958	1.000000	82.1
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	ACTAGCTAAGCCGTGGGTAT	CGCCAGGAATGAGAAACGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.088211	4012	4595	982-984	1.000000	81.8
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	ACAGAGAAGAGCAGCACCTA	CGCCAGGAATGAGAAACGTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.074332	4012	4595	1420-1422	1.000000	81.5
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	ACAGAGAAGAGCAGCACCTA	TACGCCAGGAATGAGAAACG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.074332	4012	4595	1422-1424	1.000000	81.5
trnT-GGU-trnK-UUU_p1	trnT-GGU-trnK-UUU	1	TACCGCTGAGTTAAAAGGGC	AGCCGAGTACTCTACCATTGA	20	21	50.0	47.6	57.9	58.0	0.0	20.3	1.117841	4668	5503	883-889	1.000000	79.7
trnT-GGU-trnK-UUU_p2	trnT-GGU-trnK-UUU	2	TACCGCTGAGTTAAAAGGGC	GCCGAGTACTCTACCATTGAG	20	21	50.0	52.4	57.9	57.6	0.0	20.3	1.460703	4668	5503	882-888	1.000000	76.9
trnT-GGU-trnK-UUU_p3	trnT-GGU-trnK-UUU	3	TACCGCTGAGTTAAAAGGGC	GCCGAGTACTCTACCATTGA	20	20	50.0	50.0	57.9	56.5	0.0	20.3	1.641655	4668	5503	882-888	1.000000	75.5
trnT-GGU-trnK-UUU_p4	trnT-GGU-trnK-UUU	4	TACCGCTGAGTTAAAAGGGC	AGCCGAGTACTCTACCATTG	20	20	50.0	50.0	57.9	56.5	0.0	20.3	1.651256	4668	5503	883-889	1.000000	75.4
trnT-GGU-trnK-UUU_p5	trnT-GGU-trnK-UUU	5	TACCGCTGAGTTAAAAGGGC	AAGCCGAGTACTCTACCATTG	20	21	50.0	47.6	57.9	57.2	0.0	20.3	1.906486	4668	5503	884-890	1.000000	73.4
trnK-UUU_p1	trnK-UUU	1	ACATCGACTGGGTTTAGTGT	AAATAGACGCCTTGCCCAAA	20	20	45.0	45.0	56.5	58.1	0.0	0.0	1.597142	5504	7905	2987-2991	0.666667	54.1
trnK-UUU_p2	trnK-UUU	2	ACATCGACTGGGTTTAGTGT	AATAGACGCCTTGCCCAAAA	20	20	45.0	45.0	56.5	58.1	0.0	0.0	1.597142	5504	7905	2986-2990	0.666667	54.1
trnK-UUU_p3	trnK-UUU	3	ACATCGACTGGGTTTAGTGT	AGACGCCTTGCCCAAAAATA	20	20	45.0	45.0	56.5	58.1	0.0	0.0	1.597142	5504	7905	2983-2987	0.666667	54.1
trnK-UUU_p4	trnK-UUU	4	ACATCGACTGGGTTTAGTGT	ATAGACGCCTTGCCCAAAAA	20	20	45.0	45.0	56.5	58.1	0.0	0.0	1.597142	5504	7905	2985-2989	0.666667	54.1
trnK-UUU_p5	trnK-UUU	5	ACATCGACTGGGTTTAGTGT	TAGACGCCTTGCCCAAAAAT	20	20	45.0	45.0	56.5	58.1	0.0	0.0	1.597142	5504	7905	2984-2988	0.666667	54.1
ycf2_p1	ycf2	1	CTCCTTTTCGGTTTTTGTCGA	GGAAGGCCGGTTCATTGTAT	21	20	42.9	50.0	57.0	57.9	0.0	1.9	2.008554	11258	11377	345-345	1.000000	64.2
ycf2_p2	ycf2	2	CTCCTTTTCGGTTTTTGTCGA	TAAGGAAGGCCGGTTCATTG	21	20	42.9	50.0	57.0	57.9	0.0	13.2	2.078555	11258	11377	348-348	1.000000	63.6
ycf2_p3	ycf2	3	CTCCTTTTCGGTTTTTGTCGA	TCCAATTTAAGGAAGGCCGG	21	20	42.9	50.0	57.0	57.9	0.0	0.0	2.093581	11258	11377	355-355	1.000000	63.5
ycf2_p4	ycf2	4	TCGGTTTTTGTCGATTGAAACC	GGAAGGCCGGTTCATTGTAT	22	20	40.9	50.0	58.0	57.9	20.6	1.9	2.097784	11258	11377	338-338	1.000000	63.5
ycf2_p5	ycf2	5	CTCCTTTTCGGTTTTTGTCGA	CCAATTTAAGGAAGGCCGGT	21	20	42.9	50.0	57.0	58.2	0.0	1.9	2.108443	11258	11377	354-354	1.000000	63.4
trnA-UGC_p1	trnA-UGC	1	TGGAGAGCACAGTACGATGA	AAATCCTAGATTCCCGGCCT	20	20	50.0	50.0	58.2	58.2	3.8	0.0	0.344724	23516	23550	206-206	1.000000	77.6
trnA-UGC_p2	trnA-UGC	2	TGGAGAGCACAGTACGATGA	AATCCTAGATTCCCGGCCTT	20	20	50.0	50.0	58.2	58.2	3.8	0.0	0.344724	23516	23550	205-205	1.000000	77.6
trnA-UGC_p3	trnA-UGC	3	TGGTAGAATCACTCGGGGAG	AAATCCTAGATTCCCGGCCT	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.402250	23516	23550	136-136	1.000000	77.4
trnA-UGC_p4	trnA-UGC	4	TGGTAGAATCACTCGGGGAG	AATCCTAGATTCCCGGCCTT	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.402250	23516	23550	135-135	1.000000	77.4
trnA-UGC_p5	trnA-UGC	5	TCGTTGGCCTCTATGGTAGA	AATCCTAGATTCCCGGCCTT	20	20	50.0	50.0	57.6	58.2	3.3	0.0	0.626320	23516	23550	148-148	1.000000	75.5
ycf1_p1	ycf1	1	TAAACCCGAAAAGGAACCCG	TGCGGCTTCTCTCTGTTTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.137800	34162	34281	899-971	1.000000	79.8
ycf1_p2	ycf1	2	TAAACCCGAAAAGGAACCCG	AAACTTGTATTTTGCGCCGG	20	20	50.0	45.0	58.1	57.9	0.0	4.2	0.164032	34162	34281	514-586	1.000000	79.7
ycf1_p3	ycf1	3	TAAACCCGAAAAGGAACCCG	GCCGGATAAACCCCAATTCT	20	20	50.0	50.0	58.1	57.9	0.0	5.0	0.172484	34162	34281	499-571	1.000000	79.6
ycf1_p4	ycf1	4	TAAACCCGAAAAGGAACCCG	TCCGCCTTTGTTAGAAGTCG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.179666	34162	34281	949-1021	1.000000	79.4
ycf1_p5	ycf1	5	TAAACCCGAAAAGGAACCCG	GCTTGGCTTTCCTCTTCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.188613	34162	34281	1054-1126	1.000000	79.4
ycf1-trnP-GGG_p1	ycf1-trnP-GGG	1	GGCTAGAAGACCTTCTTTGCA	TTGGTAGCGTGACATCATGG	21	20	47.6	50.0	57.9	58.0	24.4	3.4	1.082116	36063	36342	433-436	1.000000	71.9
ycf1-trnP-GGG_p2	ycf1-trnP-GGG	2	GGCTAGAAGACCTTCTTTGCA	GTCAACTGGGGTATAACGCA	21	20	47.6	50.0	57.9	57.6	24.4	7.4	1.449314	36063	36342	455-458	1.000000	68.9
ycf1-trnP-GGG_p3	ycf1-trnP-GGG	3	GGCTAGAAGACCTTCTTTGCA	TGTCAACTGGGGTATAACGC	21	20	47.6	50.0	57.9	57.6	24.4	0.0	1.449314	36063	36342	456-459	1.000000	68.9
ycf1-trnP-GGG_p4	ycf1-trnP-GGG	4	TCGGCTAGAAGACCTTCTTTG	TTGGTAGCGTGACATCATGG	21	20	47.6	50.0	57.4	58.0	16.4	3.4	1.617367	36063	36342	435-438	1.000000	67.6
ycf1-trnP-GGG_p5	ycf1-trnP-GGG	5	GGCTAGAAGACCTTCTTTGCA	CATCATGGGGTGGTGTAAGT	21	20	47.6	50.0	57.9	57.2	24.4	0.0	1.866297	36063	36342	421-424	1.000000	65.6
trnA-UGC_p1	trnA-UGC	1	AAATCCTAGATTCCCGGCCT	TGGAGAGCACAGTACGATGA	20	20	50.0	50.0	58.2	58.2	0.0	3.8	0.344724	42898	42932	206-206	1.000000	77.6
trnA-UGC_p2	trnA-UGC	2	AATCCTAGATTCCCGGCCTT	TGGAGAGCACAGTACGATGA	20	20	50.0	50.0	58.2	58.2	0.0	3.8	0.344724	42898	42932	205-205	1.000000	77.6
trnA-UGC_p3	trnA-UGC	3	AAATCCTAGATTCCCGGCCT	TGGTAGAATCACTCGGGGAG	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.402250	42898	42932	136-136	1.000000	77.4
trnA-UGC_p4	trnA-UGC	4	AATCCTAGATTCCCGGCCTT	TGGTAGAATCACTCGGGGAG	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.402250	42898	42932	135-135	1.000000	77.4
trnA-UGC_p5	trnA-UGC	5	AATCCTAGATTCCCGGCCTT	TCGTTGGCCTCTATGGTAGA	20	20	50.0	50.0	58.2	57.6	0.0	3.3	0.626320	42898	42932	148-148	1.000000	75.5
trnH-GUG-trnI-CAU_p1	trnH-GUG-trnI-CAU	1	TCACAATCCACTGCCTTGAG	CCATTCAGCCATGGATGC	20	18	50.0	55.6	57.8	56.2	0.0	25.4	3.996975	56737	57014	333-335	1.000000	50.7
trnH-GUG-trnI-CAU_p2	trnH-GUG-trnI-CAU	2	ACAAAATATTCTGGCCCGGG	CCATTCAGCCATGGATGC	20	18	50.0	55.6	58.2	56.2	19.1	25.4	4.031997	56737	57014	387-389	1.000000	50.0
trnH-GUG-trnI-CAU_p3	trnH-GUG-trnI-CAU	3	TTCACAATCCACTGCCTTGA	CCATTCAGCCATGGATGC	20	18	45.0	55.6	57.3	56.2	0.0	25.4	4.462471	56737	57014	334-336	1.000000	46.9
trnH-GUG-trnI-CAU_p4	trnH-GUG-trnI-CAU	4	ACAATCCACTGCCTTGAGC	CCATTCAGCCATGGATGC	19	18	52.6	55.6	58.0	56.2	0.0	25.4	4.854668	56737	57014	331-333	1.000000	43.8
trnH-GUG-trnI-CAU_p5	trnH-GUG-trnI-CAU	5	ATTCACAATCCACTGCCTTGA	CCATTCAGCCATGGATGC	21	18	42.9	55.6	57.8	56.2	0.0	25.4	4.966595	56737	57014	335-337	1.000000	42.9
rpl16_p1	rpl16	1	AGTGAAACCCAATTCGGATT	AGGGTCTGCTATTTTGTAAACCA	20	23	40.0	39.1	55.2	58.0	0.0	0.0	5.786750	60427	61608	1326-1327	1.000000	40.2
rpl16_p2	rpl16	2	CGGAATTCTACTGTGGATCTTCA	AGGGTCTGCTATTTTGTAAACCA	23	23	43.5	39.1	58.0	58.0	5.2	0.0	6.018062	60427	61608	1373-1374	1.000000	40.2
rpl16_p3	rpl16	3	AGTGAAACCCAATTCGGATT	GGGTCTGCTATTTTGTAAACCAG	20	23	40.0	43.5	55.2	57.7	0.0	0.0	6.090499	60427	61608	1325-1326	1.000000	40.2
rpl16_p4	rpl16	4	TCGGAATTCTACTGTGGATCT	AGGGTCTGCTATTTTGTAAACCA	21	23	42.9	39.1	55.9	58.0	5.2	0.0	6.118717	60427	61608	1374-1375	1.000000	40.2
rpl16_p5	rpl16	5	AGTGAAACCCAATTCGGATT	GGGTCTGCTATTTTGTAAACCA	20	22	40.0	40.9	55.2	56.6	0.0	0.0	6.175250	60427	61608	1325-1326	1.000000	40.2
psbB-ORF223_p1	psbB-ORF223	1	CGATACCAAGGCAAACCCAT	ACCTCTTCGTAACGAACACG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.279396	69833	70406	610-638	1.000000	82.0
psbB-ORF223_p2	psbB-ORF223	2	CACGGCAATTAAACGACCAG	ACCTCTTCGTAACGAACACG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.283363	69833	70406	656-684	1.000000	81.7
psbB-ORF223_p3	psbB-ORF223	3	ATGCACGGCAATTAAACGAC	ACCTCTTCGTAACGAACACG	20	20	45.0	50.0	57.7	58.0	2.7	0.0	0.298548	69833	70406	659-687	1.000000	81.6
psbB-ORF223_p4	psbB-ORF223	4	ACACGATACCAAGGCAAACC	ACCTCTTCGTAACGAACACG	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.511780	69833	70406	613-641	1.000000	80.1
psbB-ORF223_p5	psbB-ORF223	5	CGATACCAAGGCAAACCCAT	CCTCTTCGTAACGAACACGA	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.558098	69833	70406	609-637	1.000000	79.8
ycf4_p1	ycf4	1	AAGTAACCCACGATTCCACG	GCGATCACAATGGTGCTTTC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.188712	79316	79882	1196-1254	1.000000	82.6
ycf4_p2	ycf4	2	AAGTAACCCACGATTCCACG	GCTTTTCATGGAAAACGCGA	20	20	50.0	45.0	57.9	58.0	0.0	15.9	0.133837	79316	79882	1380-1436	1.000000	82.5
ycf4_p3	ycf4	3	AAGTAACCCACGATTCCACG	TCGCTTTTCATGGAAAACGC	20	20	50.0	45.0	57.9	58.0	0.0	15.9	0.133837	79316	79882	1382-1438	1.000000	82.5
ycf4_p4	ycf4	4	TTTAGGGGGAAGCGTTTCTG	GCTTTTCATGGAAAACGCGA	20	20	50.0	45.0	57.8	58.0	11.9	15.9	0.239089	79316	79882	1034-1090	1.000000	82.3
ycf4_p5	ycf4	5	TTTAGGGGGAAGCGTTTCTG	TCGCTTTTCATGGAAAACGC	20	20	50.0	45.0	57.8	58.0	11.9	15.9	0.239089	79316	79882	1036-1092	1.000000	82.3