primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	76	396	430-452	1.000000	84.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	76	396	1010-1032	1.000000	81.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	76	396	1024-1046	1.000000	81.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	76	396	1023-1045	1.000000	81.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	76	396	1025-1047	1.000000	81.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	6297	7475	1321-1361	1.000000	81.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6297	7475	1304-1344	1.000000	80.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6297	7475	1263-1303	1.000000	80.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6297	7475	1246-1286	1.000000	80.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	58.2	58.0	7.8	0.0	0.217997	6297	7475	1322-1362	1.000000	79.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.241837	8779	9515	806-812	1.000000	73.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.243225	8779	9515	805-811	1.000000	73.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.248863	8779	9515	806-812	1.000000	73.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.250251	8779	9515	805-811	1.000000	73.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.2	58.2	0.0	1.2	1.388603	8779	9515	860-866	1.000000	71.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28758	29603	941-956	1.000000	81.7
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28758	29603	939-954	1.000000	81.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	ATCCGGGAAAATTTGCACCT	ACTTCTTCCCCACACTACGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.068179	28758	29603	1018-1033	1.000000	81.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	ATCCGGGAAAATTTGCACCT	TTCTTCCCCACACTACGAGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.068179	28758	29603	1016-1031	1.000000	81.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATCCGGGAAAATTTGCACCT	CACTTCTTCCCCACACTACG	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.143095	28758	29603	1019-1034	1.000000	81.0
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCTTGCGTTTATTGCTACTGC	20	21	50.0	42.9	58.0	57.3	0.0	0.0	1.745414	29694	30705	1106-1134	1.000000	68.6
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCTTGCGTTTATTGCTACTGC	20	21	50.0	42.9	58.0	57.3	0.0	0.0	1.745414	29694	30705	1104-1132	1.000000	68.6
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TCTTGCGTTTATTGCTACTGC	20	21	55.0	42.9	57.9	57.3	0.0	0.0	1.820330	29694	30705	1103-1131	1.000000	68.0
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TCTTGCGTTTATTGCTACTGC	20	21	50.0	42.9	58.2	57.3	0.0	0.0	1.891392	29694	30705	1158-1186	1.000000	67.3
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	TTCTTGCGTTTATTGCTACTGC	20	22	50.0	40.9	58.0	57.9	0.0	0.0	2.083733	29694	30705	1105-1133	1.000000	65.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CCATGATCAACCTCCCTTCG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.046929	31729	32318	746-763	1.000000	80.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CCATGATCAACCTCCCTTCG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.144496	31729	32318	711-728	1.000000	79.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AACCTCCCTTCGTAGTACCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.148006	31729	32318	738-755	1.000000	79.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CCATGATCAACCTCCCTTCG	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	58.0	58.2	0.0	0.0	0.191862	31729	32318	743-760	1.000000	79.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AACCTCCCTTCGTAGTACCC	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.245573	31729	32318	703-720	1.000000	78.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.7	0.0	0.279423	51621	52624	1130-1135	1.000000	79.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTCCGGCCTTTTCCTAATCG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.614230	51621	52624	1149-1154	1.000000	76.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.7	0.0	0.623195	51621	52624	1129-1134	1.000000	76.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.7	0.0	0.765489	51621	52624	1118-1123	1.000000	75.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAATCCCGGAAAT	AAGGTCTACGGTTCGAGTCC	20	20	50.0	55.0	57.9	58.8	0.7	0.0	0.909965	51621	52624	1128-1133	1.000000	74.3
accD-psaI_p1	accD-psaI	1	TCGTACCGCGCAATCTTTTA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	8.7	26.9	0.033461	60061	60757	856-884	1.000000	80.8
accD-psaI_p2	accD-psaI	2	TCGTACCGCGCAATCTTTTA	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.0	57.9	8.7	26.8	0.109995	60061	60757	862-890	1.000000	80.1
accD-psaI_p3	accD-psaI	3	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.121766	60061	60757	813-841	1.000000	80.1
accD-psaI_p4	accD-psaI	4	CTCCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.129443	60061	60757	809-837	1.000000	80.0
accD-psaI_p5	accD-psaI	5	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	0.0	26.8	0.198299	60061	60757	819-847	1.000000	79.5
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	64388	65490	997-1266	1.000000	80.8
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	64388	65490	999-1268	1.000000	80.8
petA-psbJ_p3	petA-psbJ	3	CCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	20	20	55.0	50.0	58.3	57.5	0.0	0.0	0.755365	64388	65490	1082-1351	1.000000	80.0
petA-psbJ_p4	petA-psbJ	4	GACCCATTACGTGTCCAAGG	TCCGGATTAGGTTCATCCCT	20	20	55.0	50.0	58.3	57.5	0.0	0.0	0.755365	64388	65490	1084-1353	1.000000	80.0
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	64388	65490	996-1265	1.000000	80.0
rrn16_p1	rrn16	1	CGAATCCGCTTTGTCTACGA	AACTCAGACGTAGCTCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.169748	101207	102697	1622-1626	1.000000	83.8
rrn16_p2	rrn16	2	CGAATCCGCTTTGTCTACGA	TGTTTTGGGTGTGAAGCAGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.249849	101207	102697	1597-1601	1.000000	83.2
rrn16_p3	rrn16	3	CGAATCCGCTTTGTCTACGA	GTTTTGGGTGTGAAGCAGTG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.304182	101207	102697	1596-1600	1.000000	82.8
rrn16_p4	rrn16	4	CGAATCCGCTTTGTCTACGA	TCAGACGTAGCTCCCTTCTT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.313366	101207	102697	1619-1623	1.000000	82.6
rrn16_p5	rrn16	5	GAACTCCGGGCGAATATGAA	AACTCAGACGTAGCTCCCTT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.308191	101207	102697	1683-1687	1.000000	82.5
rrn23_p1	rrn23	1	AAACTTCTGTCGCTCCATCC	TCCATCCATTTAGCAGCACC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.157742	131659	134467	2985-2994	1.000000	81.1
rrn23_p2	rrn23	2	AAACTTCTGTCGCTCCATCC	CCATCCATTTAGCAGCACCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.163575	131659	134467	2984-2993	1.000000	81.1
rrn23_p3	rrn23	3	AAACTTCTGTCGCTCCATCC	GCACCTTAGGATGGCATAGC	20	20	50.0	55.0	57.9	58.5	0.0	4.8	0.572475	131659	134467	2970-2979	1.000000	77.8
rrn23_p4	rrn23	4	TCAAAACTTCTGTCGCTCCA	TCCATCCATTTAGCAGCACC	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.670163	131659	134467	2988-2997	1.000000	77.0
rrn23_p5	rrn23	5	TCAAAACTTCTGTCGCTCCA	CCATCCATTTAGCAGCACCT	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.675996	131659	134467	2987-2996	1.000000	77.0
rrn16_p1	rrn16	1	AACTCAGACGTAGCTCCCTT	CGAATCCGCTTTGTCTACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.169748	136914	138404	1622-1626	1.000000	83.7
rrn16_p2	rrn16	2	TGTTTTGGGTGTGAAGCAGT	CGAATCCGCTTTGTCTACGA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.249849	136914	138404	1597-1601	1.000000	83.1
rrn16_p3	rrn16	3	GTTTTGGGTGTGAAGCAGTG	CGAATCCGCTTTGTCTACGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.304182	136914	138404	1596-1600	1.000000	82.7
rrn16_p4	rrn16	4	TCAGACGTAGCTCCCTTCTT	CGAATCCGCTTTGTCTACGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.313366	136914	138404	1619-1623	1.000000	82.5
rrn16_p5	rrn16	5	AACTCAGACGTAGCTCCCTT	GAACTCCGGGCGAATATGAA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.308191	136914	138404	1683-1687	1.000000	82.4