primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoB_p1	rpoC2-rpoB	1	CCCTCCCTGTCATCGTATCT	TGCCCCCTTATCTACTACGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.007876	29589	30388	1822-1822	0.500000	59.9
rpoC2-rpoB_p2	rpoC2-rpoB	2	TCCCTCCCTGTCATCGTATC	TGCCCCCTTATCTACTACGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.008572	29589	30388	1823-1823	0.500000	59.9
rpoC2-rpoB_p3	rpoC2-rpoB	3	GTCTTGTCAACAACGCTTCG	TGCCCCCTTATCTACTACGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.010559	29589	30388	1227-1227	0.500000	59.9
rpoC2-rpoB_p4	rpoC2-rpoB	4	CCCTCCCTGTCATCGTATCT	GAACTACTGAAGCTGCTCCC	20	20	55.0	55.0	58.0	58.0	0.0	1.0	0.019643	29589	30388	1664-1664	0.500000	59.8
rpoC2-rpoB_p5	rpoC2-rpoB	5	CCCTCCCTGTCATCGTATCT	GGAACTACTGAAGCTGCTCC	20	20	55.0	55.0	58.0	58.0	0.0	1.0	0.019643	29589	30388	1665-1665	0.500000	59.8
ycf1_p1	ycf1	1	AATTTCCCGAATGGTCCGAG	AGTTGTTTTCGGGGAGAACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061331	124018	124137	295-295	0.500000	59.5
ycf1_p2	ycf1	2	GACGATTTGGAACACGAGGA	AGTTGTTTTCGGGGAGAACC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097491	124018	124137	908-908	0.500000	59.2
ycf1_p3	ycf1	3	ACGATTTGGAACACGAGGAG	AGTTGTTTTCGGGGAGAACC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103230	124018	124137	907-907	0.500000	59.2
ycf1_p4	ycf1	4	TCAAGCAAACGTACACTCCC	AGTTGTTTTCGGGGAGAACC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.149246	124018	124137	642-642	0.500000	58.8
ycf1_p5	ycf1	5	AGGAGGGAGAAATCGACGAA	AGTTGTTTTCGGGGAGAACC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.174292	124018	124137	892-892	0.500000	58.6
ycf1-rps15_p1	ycf1-rps15	1	ATGGAAGCGGGGTTTGATTT	GATGAGTCGAAGAACGCCAT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.134914	127019	127437	1624-1624	0.500000	58.9
ycf1-rps15_p2	ycf1-rps15	2	ATGGAAGCGGGGTTTGATTT	CTTCACCGTCGAGAGTAACG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.183163	127019	127437	1643-1643	0.500000	58.5
ycf1-rps15_p3	ycf1-rps15	3	ATGGAAGCGGGGTTTGATTT	GGACCGAGCCATAACAGATG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.191729	127019	127437	1896-1896	0.500000	58.5
ycf1-rps15_p4	ycf1-rps15	4	ATGGAAGCGGGGTTTGATTT	ACGGTTATTGCTTCGGTGAA	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.235660	127019	127437	1785-1785	0.500000	58.1
ycf1-rps15_p5	ycf1-rps15	5	GCCCCTAGACGTCGTATAGA	GATGAGTCGAAGAACGCCAT	20	20	55.0	50.0	57.8	58.1	24.8	0.0	0.247983	127019	127437	1162-1162	0.500000	58.0
rps15_p1	rps15	1	GCCCCTAGACGTCGTATAGA	AGCCATGAGGTAAATCAGCG	20	20	55.0	50.0	57.8	58.0	24.8	0.0	0.216836	127438	127710	1586-1586	0.500000	58.3
rps15_p2	rps15	2	GCCCCTAGACGTCGTATAGA	CATGAGGTAAATCAGCGGCT	20	20	55.0	50.0	57.8	58.0	24.8	0.0	0.216836	127438	127710	1583-1583	0.500000	58.3
rps15_p3	rps15	3	AGCCCCTAGACGTCGTATAG	AGCCATGAGGTAAATCAGCG	20	20	55.0	50.0	57.8	58.0	18.0	0.0	0.222913	127438	127710	1587-1587	0.500000	58.2
rps15_p4	rps15	4	AGCCCCTAGACGTCGTATAG	CATGAGGTAAATCAGCGGCT	20	20	55.0	50.0	57.8	58.0	18.0	0.0	0.222913	127438	127710	1584-1584	0.500000	58.2
rps15_p5	rps15	5	GCCCCTAGACGTCGTATAGA	GATGAGTCGAAGAACGCCAT	20	20	55.0	50.0	57.8	58.1	24.8	0.0	0.247983	127438	127710	1162-1162	0.500000	58.0
ndhH_p1	ndhH	1	CGCCAAAGGATGCTGTCTTA	ACAAGGTGAGAGCCAAAAGG	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.275279	127815	128996	1751-1751	0.500000	57.8
ndhH_p2	ndhH	2	CGCCAAAGGATGCTGTCTTA	GTTGTTGTATCCCTGCCGAT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.300549	127815	128996	1564-1564	0.500000	57.6
ndhH_p3	ndhH	3	TTGGGAAAACGCCAAAGGAT	ACAAGGTGAGAGCCAAAAGG	20	20	45.0	50.0	58.3	58.0	0.0	0.0	0.302302	127815	128996	1760-1760	0.500000	57.6
ndhH_p4	ndhH	4	GAAAACGCCAAAGGATGCTG	ACAAGGTGAGAGCCAAAAGG	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.311414	127815	128996	1756-1756	0.500000	57.5
ndhH_p5	ndhH	5	CGCCAAAGGATGCTGTCTTA	TGTCTTAACGAATCGCACGT	20	20	50.0	45.0	58.3	57.9	0.0	1.2	0.316849	127815	128996	1954-1954	0.500000	57.5
ndhA_p1	ndhA	1	TAGGGGATCAGAACGGGTTT	ATTTCCAATCAACGACGGGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.233068	128998	131307	2804-2804	0.500000	58.1
ndhA_p2	ndhA	2	TAGGGGATCAGAACGGGTTT	TAAACGACCCAGGGCAATTT	20	20	50.0	45.0	58.0	57.7	0.0	4.9	0.334940	128998	131307	2986-2986	0.500000	57.3
ndhA_p3	ndhA	3	ATAGGGGATCAGAACGGGTT	ATTTCCAATCAACGACGGGT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.386183	128998	131307	2805-2805	0.500000	56.9
ndhA_p4	ndhA	4	TCTAGAAGGAATTCCGGGGG	ATTTCCAATCAACGACGGGT	20	20	55.0	45.0	58.2	57.8	18.8	0.0	0.391648	128998	131307	2995-2995	0.500000	56.9
ndhA_p5	ndhA	5	AACGGGTTTCCTTGGAGATG	ATTTCCAATCAACGACGGGT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.394574	128998	131307	2793-2793	0.500000	56.8
ndhI_p1	ndhI	1	ACTTGGGCGGTTGGAATATC	ACCAGCAAAGTAACCCCATC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.268724	131390	131893	1546-1546	0.500000	57.9
ndhI_p2	ndhI	2	ACTTGGGCGGTTGGAATATC	AGGGTGTGAATTAATGCGGG	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.290075	131390	131893	1115-1115	0.500000	57.7
ndhI_p3	ndhI	3	ACAGTTCTTTACTTGGGCGG	ACCAGCAAAGTAACCCCATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.323733	131390	131893	1556-1556	0.500000	57.4
ndhI_p4	ndhI	4	CAGTTCTTTACTTGGGCGGT	ACCAGCAAAGTAACCCCATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.323733	131390	131893	1555-1555	0.500000	57.4
ndhI_p5	ndhI	5	GGTTCTGGAATAGCGATGGG	ACCAGCAAAGTAACCCCATC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.331707	131390	131893	1863-1863	0.500000	57.3
ndhI-ndhG_p1	ndhI-ndhG	1	ACCCGTCGTTGATTGGAAAT	ACCAGCAAAGTAACCCCATC	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.410144	131894	132276	962-962	0.500000	56.7
ndhI-ndhG_p2	ndhI-ndhG	2	ACCCGTCGTTGATTGGAAAT	ATCCCCAAGGGTCCAAAGAT	20	20	45.0	50.0	57.8	58.3	0.0	4.1	0.495327	131894	132276	945-945	0.500000	56.0
ndhI-ndhG_p3	ndhI-ndhG	3	AAATTGCCCTGGGTCGTTTA	ACCAGCAAAGTAACCCCATC	20	20	45.0	50.0	57.7	57.8	15.6	0.0	0.512015	131894	132276	780-780	0.500000	55.9
ndhI-ndhG_p4	ndhI-ndhG	4	ACCCGTCGTTGATTGGAAAT	AGTTGTGCAGCAGCTACAAA	20	20	45.0	45.0	57.8	58.3	0.0	11.6	0.515062	131894	132276	842-842	0.500000	55.9
ndhI-ndhG_p5	ndhI-ndhG	5	ACCCGTCGTTGATTGGAAAT	TCCCGTACCATGACGTATCT	20	20	45.0	50.0	57.8	57.6	0.0	6.2	0.547558	131894	132276	1021-1021	0.500000	55.6
ndhG_p1	ndhG	1	ACCCGTCGTTGATTGGAAAT	TCGGATCGAGTCATGTGAGA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.232588	132277	132807	1881-1881	0.500000	58.1
ndhG_p2	ndhG	2	CCCGCATTAATTCACACCCT	TCGGATCGAGTCATGTGAGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.275679	132277	132807	1370-1370	0.500000	57.8
ndhG_p3	ndhG	3	AAATTGCCCTGGGTCGTTTA	TCGGATCGAGTCATGTGAGA	20	20	45.0	50.0	57.7	58.0	15.6	0.0	0.334460	132277	132807	1699-1699	0.500000	57.3
ndhG_p4	ndhG	4	CTACCCGTCGTTGATTGGAA	TCGGATCGAGTCATGTGAGA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.411927	132277	132807	1883-1883	0.500000	56.7
ndhG_p5	ndhG	5	GTCCAATAACTGTACCCGCA	TCGGATCGAGTCATGTGAGA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.428349	132277	132807	1384-1384	0.500000	56.6
psaC_p1	psaC	1	TCTCACATGACTCGATCCGA	GGACCAGAAAAACCCGTTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061331	133628	133873	498-498	0.500000	59.5
psaC_p2	psaC	2	TCTCACATGACTCGATCCGA	CGTCAGCAAAATGGGTCCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.146337	133628	133873	797-797	0.500000	58.8
psaC_p3	psaC	3	TCTCACATGACTCGATCCGA	TTGGACCAGAAAAACCCGTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.185307	133628	133873	500-500	0.500000	58.5
psaC_p4	psaC	4	TCTCACATGACTCGATCCGA	GTGATAAACCCCGTCAGCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.222312	133628	133873	808-808	0.500000	58.2
psaC_p5	psaC	5	TCTCACATGACTCGATCCGA	CGTTTCTTTCCTCCCCACAT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.238760	133628	133873	997-997	0.500000	58.1
ndhD_p1	ndhD	1	TCTGCTCCAAGAACAGAGGA	TCAAAGTAATGGCTTCGCGA	20	20	50.0	45.0	58.0	57.9	0.0	3.7	0.102957	134002	135504	1928-1928	0.500000	59.2
ndhD_p2	ndhD	2	GGGACGGGTGTAAAGCTAAG	TCAAAGTAATGGCTTCGCGA	20	20	55.0	45.0	58.0	57.9	0.0	3.7	0.107449	134002	135504	1957-1957	0.500000	59.1
ndhD_p3	ndhD	3	AGAACGGGTTTTTCTGGTCC	TCAAAGTAATGGCTTCGCGA	20	20	50.0	45.0	58.0	57.9	0.0	3.7	0.110445	134002	135504	1714-1714	0.500000	59.1
ndhD_p4	ndhD	4	AGATGTGAATCCGCTTGTCC	TCAAAGTAATGGCTTCGCGA	20	20	50.0	45.0	58.0	57.9	0.0	3.7	0.117339	134002	135504	1892-1892	0.500000	59.1
ndhD_p5	ndhD	5	TCTCACATGACTCGATCCGA	TCAAAGTAATGGCTTCGCGA	20	20	50.0	45.0	58.0	57.9	0.0	3.7	0.125888	134002	135504	2192-2192	0.500000	59.0
ccsA_p1	ccsA	1	CTTCGCGATGGATTACACGA	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.2	58.0	10.9	0.0	0.172709	135773	136738	1250-1250	0.500000	58.6
ccsA_p2	ccsA	2	TCGCGATGGATTACACGAAG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.2	58.0	3.7	0.0	0.172709	135773	136738	1248-1248	0.500000	58.6
ccsA_p3	ccsA	3	CTTCGCGATGGATTACACGA	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.2	58.0	10.9	0.0	0.174080	135773	136738	1249-1249	0.500000	58.6
ccsA_p4	ccsA	4	TCGCGATGGATTACACGAAG	TACTTCACTGGCGACGATTG	20	20	50.0	50.0	58.2	58.0	3.7	0.0	0.174080	135773	136738	1316-1316	0.500000	58.6
ccsA_p5	ccsA	5	TCGCGATGGATTACACGAAG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.2	58.0	3.7	0.0	0.174080	135773	136738	1247-1247	0.500000	58.6
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TGGCGCGGTTATTACGTTTA	ATTCGATGGACTCTGTTGGA	20	20	45.0	45.0	58.0	56.3	8.8	0.0	1.746924	136947	137737	2029-2029	0.500000	46.0
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TGGCGCGGTTATTACGTTTA	TCGATGGACTCTGTTGGATT	20	20	45.0	45.0	58.0	56.3	8.8	0.0	1.746924	136947	137737	2027-2027	0.500000	46.0
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TGGCGCGGTTATTACGTTTA	TTCGATGGACTCTGTTGGAT	20	20	45.0	45.0	58.0	56.3	8.8	0.0	1.746924	136947	137737	2028-2028	0.500000	46.0
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGCGTGTCTACCGATTTCAC	TCGATGGACTCTGTTGGATT	20	20	50.0	45.0	58.0	56.3	0.0	0.0	1.752943	136947	137737	1530-1530	0.500000	46.0
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AGCGTGTCTACCGATTTCAC	TTCGATGGACTCTGTTGGAT	20	20	50.0	45.0	58.0	56.3	0.0	0.0	1.752943	136947	137737	1531-1531	0.500000	46.0
rpl32_p1	rpl32	1	TCGTCGCCAGTGAAGTAGTA	ATTCGATGGACTCTGTTGGA	20	20	50.0	45.0	58.2	56.3	0.0	0.0	1.932877	137738	137896	1461-1461	0.500000	44.5
rpl32_p2	rpl32	2	TCGTCGCCAGTGAAGTAGTA	TCGATGGACTCTGTTGGATT	20	20	50.0	45.0	58.2	56.3	0.0	0.0	1.932877	137738	137896	1459-1459	0.500000	44.5
rpl32_p3	rpl32	3	TCGTCGCCAGTGAAGTAGTA	TTCGATGGACTCTGTTGGAT	20	20	50.0	45.0	58.2	56.3	0.0	0.0	1.932877	137738	137896	1460-1460	0.500000	44.5
rpl32_p4	rpl32	4	TCGTCGCCAGTGAAGTAGTA	AGGAAACGCTATGATCTCCA	20	20	50.0	45.0	58.2	56.0	0.0	0.0	2.156312	137738	137896	1627-1627	0.500000	42.7
rpl32_p5	rpl32	5	TCGTCGCCAGTGAAGTAGTA	TCGATGGACTCTGTTGGATTT	20	21	50.0	42.9	58.2	57.0	0.0	0.0	2.168836	137738	137896	1459-1459	0.500000	42.6
rpl32-ndhF_p1	rpl32-ndhF	1	GGTAGGTATTGGTCTGGGCT	ATGAAGGGGAAATTGGGCAG	20	20	55.0	50.0	58.5	58.1	0.0	0.0	0.621680	137897	139130	2330-2330	0.500000	55.0
rpl32-ndhF_p2	rpl32-ndhF	2	GGTAGGTATTGGTCTGGGCT	GAAGGGGAAATTGGGCAGAT	20	20	55.0	50.0	58.5	57.8	0.0	0.0	0.657101	137897	139130	2328-2328	0.500000	54.7
rpl32-ndhF_p3	rpl32-ndhF	3	GGTAGGTATTGGTCTGGGCT	CGGTCGTGTGAACCAAAAAC	20	20	55.0	50.0	58.5	58.2	0.0	0.0	0.674135	137897	139130	2096-2096	0.500000	54.6
rpl32-ndhF_p4	rpl32-ndhF	4	GGTAGGTATTGGTCTGGGCT	GGTCGTGTGAACCAAAAACC	20	20	55.0	50.0	58.5	57.8	0.0	0.0	0.689536	137897	139130	2095-2095	0.500000	54.5
rpl32-ndhF_p5	rpl32-ndhF	5	AGGTATTGGTCTGGGCTAGT	ATGAAGGGGAAATTGGGCAG	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.700322	137897	139130	2327-2327	0.500000	54.4
ndhF_p1	ndhF	1	GAGCCGGTAACTCAGAAACA	GCAGAATACCGTCACCCATT	20	20	50.0	50.0	57.5	58.0	1.9	0.0	0.488996	139131	141389	2643-2643	0.500000	56.1
ndhF_p2	ndhF	2	GAGCCGGTAACTCAGAAACA	AAGAGGGATCCACCGAAGAA	20	20	50.0	50.0	57.5	58.0	1.9	15.2	0.497988	139131	141389	2532-2532	0.500000	56.0
ndhF_p3	ndhF	3	GAGCCGGTAACTCAGAAACA	AGGGATCCACCGAAGAAGAT	20	20	50.0	50.0	57.5	57.8	1.9	15.2	0.633125	139131	141389	2529-2529	0.500000	54.9
ndhF_p4	ndhF	4	AGAGCCGGTAACTCAGAAAC	GCAGAATACCGTCACCCATT	20	20	50.0	50.0	57.3	58.0	1.9	0.0	0.783902	139131	141389	2644-2644	0.500000	53.7
ndhF_p5	ndhF	5	AGAGCCGGTAACTCAGAAAC	AAGAGGGATCCACCGAAGAA	20	20	50.0	50.0	57.3	58.0	1.9	15.2	0.792893	139131	141389	2533-2533	0.500000	53.7