primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	rpl2-rpl23	1	CACTTGACTGTCTACGGCTC	GTAAAGGGTAGAAGGGTGCG	20	20	55.0	55.0	58.0	58.0	9.5	0.0	0.035975	1584	1601	198-198	1.000000	80.1
rpl2-rpl23_p2	rpl2-rpl23	2	CCACAACGATGCTGTCCATA	GTAAAGGGTAGAAGGGTGCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.042712	1584	1601	245-245	1.000000	80.0
rpl2-rpl23_p3	rpl2-rpl23	3	CACTTGACTGTCTACGGCTC	TAAAGGGTAGAAGGGTGCGA	20	20	55.0	50.0	58.0	58.1	9.5	0.0	0.074650	1584	1601	197-197	1.000000	79.8
rpl2-rpl23_p4	rpl2-rpl23	4	CCACAACGATGCTGTCCATA	TAAAGGGTAGAAGGGTGCGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.081387	1584	1601	244-244	1.000000	79.7
rpl2-rpl23_p5	rpl2-rpl23	5	GACTGTCTACGGCTCCATTG	GTAAAGGGTAGAAGGGTGCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.092053	1584	1601	193-193	1.000000	79.7
ndhF-rpl32_p1	ndhF-rpl32	1	ACACACTCCAACCAATTCCC	CGAGAAAATTCCGCGAATCG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.012799	27965	28449	1168-1168	0.500000	59.9
ndhF-rpl32_p2	ndhF-rpl32	2	CACACTCCAACCAATTCCCA	CGAGAAAATTCCGCGAATCG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.014219	27965	28449	1167-1167	0.500000	59.9
ndhF-rpl32_p3	ndhF-rpl32	3	ACCAATCCCAACATTGCAGT	CGAGAAAATTCCGCGAATCG	20	20	45.0	50.0	58.0	58.0	0.0	8.2	0.022185	27965	28449	1113-1113	0.500000	59.8
ndhF-rpl32_p4	ndhF-rpl32	4	CCACAGGAAACAGCAGAAGT	CGAGAAAATTCCGCGAATCG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.035303	27965	28449	791-791	0.500000	59.7
ndhF-rpl32_p5	ndhF-rpl32	5	GCAACCGAACCGCAAAATAA	CGAGAAAATTCCGCGAATCG	20	20	45.0	50.0	57.9	58.0	0.0	8.2	0.061457	27965	28449	1413-1413	0.500000	59.5
rpl32_p1	rpl32	1	CCACAGGAAACAGCAGAAGT	AAGCACGGAACCATGTGAAT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.117428	28450	28626	1494-1524	1.000000	84.7
rpl32_p2	rpl32	2	CCACAGGAAACAGCAGAAGT	GATAAGATGCCATGCCGCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126269	28450	28626	1116-1168	1.000000	84.5
rpl32_p3	rpl32	3	CCACAGGAAACAGCAGAAGT	ACGGAACCATGTGAATGAGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.140988	28450	28626	1490-1520	1.000000	84.5
rpl32_p4	rpl32	4	CCACAGGAAACAGCAGAAGT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.151405	28450	28626	1063-1115	1.000000	84.5
rpl32_p5	rpl32	5	CCACAGGAAACAGCAGAAGT	CGAGAAAATTCCGCGAATCG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.035303	28450	28626	791-791	0.500000	59.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGACAGCACTGAAAGCTTT	AAGTTGCAAAGGCTTGGGTA	20	20	45.0	45.0	57.9	57.9	12.5	0.0	0.137791	28627	28894	917-937	1.000000	84.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGGACAGCACTGAAAGCTTT	AGTTGCAAAGGCTTGGGTAA	20	20	45.0	45.0	57.9	57.9	12.5	0.0	0.137791	28627	28894	916-936	1.000000	84.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTGGACAGCACTGAAAGCTT	AAGTTGCAAAGGCTTGGGTA	20	20	45.0	45.0	57.9	57.9	4.3	0.0	0.137791	28627	28894	918-938	1.000000	84.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTGGACAGCACTGAAAGCTT	AGTTGCAAAGGCTTGGGTAA	20	20	45.0	45.0	57.9	57.9	4.3	0.0	0.137791	28627	28894	917-937	1.000000	84.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGGACAGCACTGAAAGCTTT	AAGCACGGAACCATGTGAAT	20	20	45.0	45.0	57.9	58.1	12.5	0.0	0.147438	28627	28894	840-860	1.000000	84.0
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	CGATCGAAAACGATAGATGGG	20	21	50.0	47.6	57.9	56.7	0.0	10.3	2.436707	28975	29092	209-231	1.000000	66.3
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TAGACACGCTGCTCTTAGGA	ACGATCGAAAACGATAGATGGG	20	22	50.0	45.5	57.9	58.4	0.0	24.3	2.484740	28975	29092	210-232	1.000000	65.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	TAGACACGCTGCTCTTAGGA	CGATCGAAAACGATAGATGGGT	20	22	50.0	45.5	57.9	58.4	0.0	10.3	2.484740	28975	29092	209-231	1.000000	65.9
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAATTGGTAGACACGCTGCT	CGATCGAAAACGATAGATGGG	20	21	45.0	47.6	57.8	56.7	0.0	10.3	2.497434	28975	29092	216-238	1.000000	65.8
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTAGACACGCTGCTCTTAGG	CGATCGAAAACGATAGATGGG	20	21	55.0	47.6	57.8	56.7	0.0	10.3	2.508724	28975	29092	210-232	1.000000	65.7
ndhG-ndhI_p1	ndhG-ndhI	1	ATGTAGCGATTCGGGCAAAT	TCAACAAACAATCCGAGCCA	20	20	45.0	45.0	58.0	58.0	8.3	0.0	0.058416	33590	33985	855-871	1.000000	85.4
ndhG-ndhI_p2	ndhG-ndhI	2	ATGTAGCGATTCGGGCAAAT	CAACAAACAATCCGAGCCAC	20	20	45.0	50.0	58.0	57.9	8.3	0.0	0.083158	33590	33985	854-870	1.000000	85.2
ndhG-ndhI_p3	ndhG-ndhI	3	GTGCTGCAGCTACAAAATGG	TCAACAAACAATCCGAGCCA	20	20	50.0	45.0	58.0	58.0	36.6	0.0	0.039037	33590	33985	1015-1031	1.000000	84.8
ndhG-ndhI_p4	ndhG-ndhI	4	GTGCTGCAGCTACAAAATGG	CAACAAACAATCCGAGCCAC	20	20	50.0	50.0	58.0	57.9	36.6	0.0	0.063779	33590	33985	1014-1030	1.000000	84.7
ndhG-ndhI_p5	ndhG-ndhI	5	AACAAACGCTCTTTCACCCT	TCAACAAACAATCCGAGCCA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.078517	33590	33985	1480-1480	0.500000	59.4
ndhA_p1	ndhA	1	TTGTTTAAGACAAGGTTTGAAT	TGGGAGAAGTTGATCGTTGA	22	20	27.3	45.0	52.4	56.5	0.0	0.0	9.109999	34552	36938	2388-2450	1.000000	44.8
ndhA_p2	ndhA	2	AGACAAGGTTTGAATATAGAAGATCA	TGGGAGAAGTTGATCGTTGA	26	20	30.8	45.0	56.2	56.5	0.0	0.0	9.302905	34552	36938	2381-2443	1.000000	44.8
ndhA_p3	ndhA	3	TTGTTTAAGACAAGGTTTGAAT	TGGGAGAAGTTGATCGTTGAA	22	21	27.3	42.9	52.4	57.2	0.0	0.0	9.359801	34552	36938	2388-2450	1.000000	44.8
ndhA_p4	ndhA	4	AGACAAGGTTTGAATATAGAAGATCA	TGGGAGAAGTTGATCGTTGAA	26	21	30.8	42.9	56.2	57.2	0.0	0.0	9.552707	34552	36938	2381-2443	1.000000	44.8
ndhA_p5	ndhA	5	TTGTTTAAGACAAGGTTTGAAT	ATGGGAGAAGTTGATCGTTGA	22	21	27.3	42.9	52.4	57.0	0.0	0.0	9.578465	34552	36938	2389-2451	1.000000	44.8
rps15-ycf1_p1	rps15-ycf1	1	AACACCATGCATTGATGGGT	TTATCGAACCAGTGCGTCTG	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.067824	38509	38846	1369-1396	1.000000	85.5
rps15-ycf1_p2	rps15-ycf1	2	TGCATTGATGGGTGATGAGG	TTATCGAACCAGTGCGTCTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070710	38509	38846	1362-1389	1.000000	85.5
rps15-ycf1_p3	rps15-ycf1	3	TCCCGGGTCTTCTTCTCTTT	TTATCGAACCAGTGCGTCTG	20	20	50.0	50.0	58.0	58.0	22.9	0.0	0.034837	38509	38846	994-994	0.500000	59.7
rps15-ycf1_p4	rps15-ycf1	4	TCCCGGGTCTTCTTCTCTTT	GAACCAGTGCGTCTGTCTAG	20	20	50.0	55.0	58.0	58.0	22.9	7.7	0.041663	38509	38846	989-989	0.500000	59.7
rps15-ycf1_p5	rps15-ycf1	5	GCATTGATGGGTGATGAGGT	TTATCGAACCAGTGCGTCTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.072171	38509	38846	1388-1388	0.500000	59.4
ycf1_p1	ycf1	1	CAGACGCACTGGTTCGATAA	GGAGCACGGGGAATATTTCA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.351047	39827	39946	1179-1179	0.500000	57.2
ycf1_p2	ycf1	2	CAGACGCACTGGTTCGATAA	TGGAGCACGGGGAATATTTC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.351047	39827	39946	1180-1180	0.500000	57.2
ycf1_p3	ycf1	3	CCCGTTCGACGTCACATAAT	GGAGCACGGGGAATATTTCA	20	20	50.0	50.0	58.1	57.7	17.2	0.0	0.424574	39827	39946	858-858	0.500000	56.6
ycf1_p4	ycf1	4	CCCGTTCGACGTCACATAAT	TGGAGCACGGGGAATATTTC	20	20	50.0	50.0	58.1	57.7	17.2	0.0	0.424574	39827	39946	859-859	0.500000	56.6
ycf1_p5	ycf1	5	TCTTGGTTTGAGAGGGGTGA	GGAGCACGGGGAATATTTCA	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.527417	39827	39946	821-821	0.500000	55.8
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	TCCAGAACTTTTGTTGCCCT	ACACCGAAGTATTGACGACG	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.479462	43563	44559	1796-1796	0.500000	56.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TCCAGAACTTTTGTTGCCCT	TGCCCTACTCTTGGAATCCA	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.487186	43563	44559	2080-2080	0.500000	56.1
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	TCCAGAACTTTTGTTGCCCT	GGCAGAATAGCAGAGCAAGT	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.488130	43563	44559	2013-2013	0.500000	56.1
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TCCAGAACTTTTGTTGCCCT	GGTAATTGTGGCCTCTCAGG	20	20	45.0	55.0	57.5	58.0	0.0	3.3	0.496723	43563	44559	1928-1928	0.500000	56.0
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	TCCAGAACTTTTGTTGCCCT	AATGCGTTCCTCGTCATGAA	20	20	45.0	45.0	57.5	57.9	0.0	0.0	0.545907	43563	44559	1966-1966	0.500000	55.6
rpl23-rpl2_p1	rpl23-rpl2	1	GTAAAGGGTAGAAGGGTGCG	CACTTGACTGTCTACGGCTC	20	20	55.0	55.0	58.0	58.0	0.0	9.5	0.035975	67856	67873	198-198	1.000000	80.1
rpl23-rpl2_p2	rpl23-rpl2	2	GTAAAGGGTAGAAGGGTGCG	CCACAACGATGCTGTCCATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.042712	67856	67873	245-245	1.000000	80.0
rpl23-rpl2_p3	rpl23-rpl2	3	TAAAGGGTAGAAGGGTGCGA	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	58.1	58.0	0.0	9.5	0.074650	67856	67873	197-197	1.000000	79.8
rpl23-rpl2_p4	rpl23-rpl2	4	TAAAGGGTAGAAGGGTGCGA	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081387	67856	67873	244-244	1.000000	79.7
rpl23-rpl2_p5	rpl23-rpl2	5	GTAAAGGGTAGAAGGGTGCG	GACTGTCTACGGCTCCATTG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.092053	67856	67873	193-193	1.000000	79.7
trnK-UUU_p1	trnK-UUU	1	TGGCTTCGCTTATGATATGCA	CATAGGGGACAATGGGGGTA	21	20	42.9	55.0	57.9	58.2	10.4	0.0	1.296787	71181	73867	2860-2860	0.500000	49.6
trnK-UUU_p2	trnK-UUU	2	GGCTTCGCTTATGATATGCAC	CATAGGGGACAATGGGGGTA	21	20	47.6	55.0	57.5	58.2	10.4	0.0	1.634155	71181	73867	2859-2859	0.500000	46.9
trnK-UUU_p3	trnK-UUU	3	TGGCTTCGCTTATGATATGCA	TGATGATCTACGCACCCTGT	21	20	42.9	50.0	57.9	58.5	10.4	0.0	1.639454	71181	73867	2804-2804	0.500000	46.9
trnK-UUU_p4	trnK-UUU	4	TGGCTTCGCTTATGATATGCA	TTGATGATCTACGCACCCTG	21	20	42.9	50.0	57.9	57.4	10.4	0.0	1.726835	71181	73867	2805-2805	0.500000	46.2
trnK-UUU_p5	trnK-UUU	5	GGCTTCGCTTATGATATGCAC	TGATGATCTACGCACCCTGT	21	20	47.6	50.0	57.5	58.5	10.4	0.0	1.976822	71181	73867	2803-2803	0.500000	44.2