primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK_p1	matK	1	TTCGTATTGCCTGAACATTT	CCTTGTTTTGACTGTATCGCA	20	21	35.0	42.9	53.5	56.8	0.0	0.0	6.676627	2179	3696	1714-1714	1.000000	40.0
matK_p2	matK	2	TTCGTATTGCCTGAACATTT	ACCTTGTTTTGACTGTATCGC	20	21	35.0	42.9	53.5	56.8	0.0	0.0	6.678661	2179	3696	1715-1715	1.000000	40.0
matK_p3	matK	3	TTCGTATTGCCTGAACATTT	CCTTGTTTTGACTGTATCGCAC	20	22	35.0	45.5	53.5	58.2	0.0	0.0	6.698767	2179	3696	1714-1714	1.000000	40.0
matK_p4	matK	4	TTCGTATTGCCTGAACATTT	CCCATGCATCATTTGATAACCC	20	22	35.0	45.5	53.5	57.7	0.0	0.0	6.770718	2179	3696	1674-1674	1.000000	40.0
matK_p5	matK	5	TTCGTATTGCCTGAACATTT	CCCCATGCATCATTTGATAACC	20	22	35.0	45.5	53.5	57.7	0.0	0.0	6.770718	2179	3696	1675-1675	1.000000	40.0
rps16_p1	rps16	1	GGAGTCATCCACACCCTCTT	CTGTGTATCGACCGAAGCAA	20	20	55.0	50.0	58.7	58.0	0.0	0.0	0.728744	5164	6316	1270-1271	1.000000	74.4
rps16_p2	rps16	2	GGAGTCATCCACACCCTCT	CTGTGTATCGACCGAAGCAA	19	20	57.9	50.0	58.0	58.0	0.0	0.0	1.010708	5164	6316	1270-1271	1.000000	72.1
rps16_p3	rps16	3	GAGTCATCCACACCCTCTTT	CTGTGTATCGACCGAAGCAA	20	20	50.0	50.0	56.8	58.0	0.0	0.0	1.170051	5164	6316	1269-1270	1.000000	70.8
rps16_p4	rps16	4	GGAGTCATCCACACCCTCTT	TGTGTATCGACCGAAGCAAT	20	20	55.0	45.0	58.7	57.3	0.0	0.0	1.385191	5164	6316	1269-1270	1.000000	69.1
rps16_p5	rps16	5	GAGTCATCCACACCCTCTTTT	CTGTGTATCGACCGAAGCAA	21	20	47.6	50.0	57.6	58.0	0.0	0.0	1.434363	5164	6316	1269-1270	1.000000	68.7
petN-psbM_p1	petN-psbM	1	TAGTTAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	30064	30789	888-888	1.000000	67.6
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30064	30789	827-827	1.000000	66.0
petN-psbM_p3	petN-psbM	3	TAGTTAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	30064	30789	888-888	1.000000	63.4
petN-psbM_p4	petN-psbM	4	TAGTTAGTCTCGCTTGGGCT	ACGTAAAAACGGTTAGCCAA	20	20	50.0	40.0	58.2	55.9	0.0	2.8	2.243001	30064	30789	838-838	1.000000	62.2
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	57.6	56.1	0.0	0.0	2.280835	30064	30789	827-827	1.000000	61.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32202	32984	886-886	1.000000	77.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32202	32984	865-865	1.000000	77.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	32202	32984	865-865	1.000000	77.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	32202	32984	844-844	1.000000	77.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	32202	32984	862-862	1.000000	77.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGATCCCTCCGTAACC	20	20	55.0	55.0	58.1	58.1	7.6	0.0	0.132161	33057	34560	1646-1646	1.000000	79.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGATCCCTCCGTAACC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.154316	33057	34560	1649-1649	1.000000	79.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ATCCCTCCGTAACCAGTCAT	20	20	55.0	50.0	58.1	57.8	7.6	0.0	0.240315	33057	34560	1640-1640	1.000000	78.4
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ATCCCTCCGTAACCAGTCAT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.262469	33057	34560	1643-1643	1.000000	78.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCCTATCTGCCCAGTGACTC	GAAACGATCCCTCCGTAACC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.286308	33057	34560	1701-1701	1.000000	78.0
psaA-pafI_p1	psaA-pafI	1	GCCCATTCCTCGAAAGAAGT	TATCGCGCTTACTCCTGGTA	20	20	50.0	50.0	57.9	58.0	4.1	5.6	0.143862	43805	44505	850-851	1.000000	78.9
psaA-pafI_p2	psaA-pafI	2	GCCCATTCCTCGAAAGAAGT	CGCGCTTACTCCTGGTAATT	20	20	50.0	50.0	57.9	58.1	4.1	5.6	0.179410	43805	44505	847-848	1.000000	78.7
psaA-pafI_p3	psaA-pafI	3	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	57.9	58.1	4.1	11.5	0.219123	43805	44505	820-821	1.000000	78.3
psaA-pafI_p4	psaA-pafI	4	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.244113	43805	44505	812-813	1.000000	78.1
psaA-pafI_p5	psaA-pafI	5	TTGAGAAATGACCGGGTCTG	TATCGCGCTTACTCCTGGTA	20	20	50.0	50.0	57.5	58.0	26.4	5.6	0.501214	43805	44505	870-871	1.000000	76.1
pafI-trnS-GGA_p1	pafI-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	46512	47264	842-842	1.000000	71.0
pafI-trnS-GGA_p2	pafI-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	46512	47264	863-863	1.000000	70.6
pafI-trnS-GGA_p3	pafI-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	46512	47264	813-813	1.000000	70.2
pafI-trnS-GGA_p4	pafI-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	46512	47264	815-815	1.000000	69.7
pafI-trnS-GGA_p5	pafI-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	46512	47264	843-843	1.000000	68.7
accD_p1	accD	1	TGATACTTCGGGGTCTCACA	TCTGCATGATTCTTTCGACA	20	20	50.0	40.0	57.8	55.1	0.0	0.0	3.127145	59761	61260	1662-1662	1.000000	55.0
accD_p2	accD	2	TGATACTTCGGGGTCTCACA	TCTGCATGATTCTTTCGACAA	20	21	50.0	38.1	57.8	55.9	0.0	0.0	3.320083	59761	61260	1662-1662	1.000000	53.5
accD_p3	accD	3	AGTGATACTTCGGGGTCTCA	TCTGCATGATTCTTTCGACA	20	20	50.0	40.0	57.5	55.1	0.0	0.0	3.428650	59761	61260	1664-1664	1.000000	52.6
accD_p4	accD	4	GTGATACTTCGGGGTCTCAC	TCTGCATGATTCTTTCGACA	20	20	55.0	40.0	57.4	55.1	9.8	0.0	3.482729	59761	61260	1663-1663	1.000000	52.2
accD_p5	accD	5	TGATACTTCGGGGTCTCACA	ATCTGCATGATTCTTTCGACA	20	21	50.0	38.1	57.8	55.7	0.0	0.0	3.541292	59761	61260	1663-1663	1.000000	51.7
cemA_p1	cemA	1	TCGATTCATAGGCGCGATAC	ACGACCTTGAATGCTTTGCT	20	20	50.0	45.0	57.7	58.4	12.1	0.0	0.648766	63628	64326	874-874	1.000000	74.9
cemA_p2	cemA	2	CGATTCATAGGCGCGATACT	ACGACCTTGAATGCTTTGCT	20	20	50.0	45.0	57.7	58.4	12.1	0.0	0.654901	63628	64326	873-873	1.000000	74.9
cemA_p3	cemA	3	GAGACTCGATTCATAGGCGC	ACGACCTTGAATGCTTTGCT	20	20	55.0	45.0	58.3	58.4	0.0	0.0	0.698496	63628	64326	879-879	1.000000	74.5
cemA_p4	cemA	4	CAACGGATGAGGTGGATTCA	ACGACCTTGAATGCTTTGCT	20	20	50.0	45.0	57.6	58.4	0.0	0.0	0.792375	63628	64326	807-807	1.000000	73.8
cemA_p5	cemA	5	GTCAACGGATGAGGTGGATT	ACGACCTTGAATGCTTTGCT	20	20	50.0	45.0	57.6	58.4	0.0	0.0	0.794044	63628	64326	809-809	1.000000	73.7
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	TATTCCGGATTGGGTTCGTC	20	20	50.0	50.0	58.0	57.4	0.0	4.1	0.646018	65503	66600	1244-1244	1.000000	75.0
petA-psbJ_p2	petA-psbJ	2	CATTACGTGTCCAAGGCCTT	TATTCCGGATTGGGTTCGTC	20	20	50.0	50.0	58.2	57.4	13.8	4.1	0.786415	65503	66600	1241-1241	1.000000	73.8
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCCTTGTA	20	20	50.0	50.0	58.0	57.2	0.0	0.3	0.812380	65503	66600	1238-1238	1.000000	73.6
petA-psbJ_p4	petA-psbJ	4	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCCTTGTA	20	20	50.0	50.0	58.2	57.2	13.8	0.3	0.952776	65503	66600	1235-1235	1.000000	72.5
petA-psbJ_p5	petA-psbJ	5	TTACGTGTCCAAGGCCTTTT	TATTCCGGATTGGGTTCGTC	20	20	45.0	50.0	57.6	57.4	17.6	4.1	0.959337	65503	66600	1239-1239	1.000000	72.5
rpoA-rps11_p1	rpoA-rps11	1	ACTTCCACTGTAGTGTCCGA	AGAGATGCAGCATTACGAGC	20	20	50.0	50.0	58.0	58.1	10.8	9.9	0.145612	82086	82150	228-228	1.000000	79.2
rpoA-rps11_p2	rpoA-rps11	2	ACTTCCACTGTAGTGTCCGA	GAGATGCAGCATTACGAGCT	20	20	50.0	50.0	58.0	58.1	10.8	9.9	0.145612	82086	82150	227-227	1.000000	79.2
rpoA-rps11_p3	rpoA-rps11	3	TTCCACTGTAGTGTCCGAGT	AGAGATGCAGCATTACGAGC	20	20	50.0	50.0	58.0	58.1	10.8	9.9	0.145612	82086	82150	226-226	1.000000	79.2
rpoA-rps11_p4	rpoA-rps11	4	TTCCACTGTAGTGTCCGAGT	GAGATGCAGCATTACGAGCT	20	20	50.0	50.0	58.0	58.1	10.8	9.9	0.145612	82086	82150	225-225	1.000000	79.2
rpoA-rps11_p5	rpoA-rps11	5	CACTTCCACTGTAGTGTCCG	GAGATGCAGCATTACGAGCT	20	20	55.0	50.0	57.9	58.1	10.8	9.9	0.202726	82086	82150	228-228	1.000000	78.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCGTTGAAAGCTTTTTCGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.172709	117242	118479	1361-1361	1.000000	78.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCGTTGAAAGCTTTTTCGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.174080	117242	118479	1362-1362	1.000000	78.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCGTTGAAAGCTTTTTCGT	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.2	58.1	14.0	0.0	0.223554	117242	118479	1401-1401	1.000000	78.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCGTTGAAAGCTTTTTCGT	TCGAACCGAGATGCTTTAGC	20	20	45.0	50.0	58.2	58.1	14.0	0.0	0.242309	117242	118479	1397-1397	1.000000	78.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCGTTGAAAGCTTTTTCGT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	14.0	0.0	0.289156	117242	118479	1371-1371	1.000000	77.7
ccsA_p1	ccsA	1	GGCGGCATAGCATCTTCTAA	CAAAAGGTTCTCCTCGGCTT	20	20	50.0	50.0	57.8	58.1	3.1	0.0	0.272272	118672	119640	1205-1205	1.000000	77.9
ccsA_p2	ccsA	2	GGCGGCATAGCATCTTCTAA	TTCAAAAGGTTCTCCTCGGC	20	20	50.0	50.0	57.8	58.1	3.1	0.0	0.277623	118672	119640	1207-1207	1.000000	77.8
ccsA_p3	ccsA	3	GGCGGCATAGCATCTTCTAA	GGTTCTCCTCGGCTTATACC	20	20	50.0	55.0	57.8	57.2	3.1	0.0	0.986036	118672	119640	1200-1200	1.000000	72.2
ccsA_p4	ccsA	4	TGGCGGCATAGCATCTTCTA	CAAAAGGTTCTCCTCGGCTT	20	20	50.0	50.0	59.0	58.1	8.0	0.0	1.054639	118672	119640	1206-1206	1.000000	71.6
ccsA_p5	ccsA	5	TGGCGGCATAGCATCTTCTA	TTCAAAAGGTTCTCCTCGGC	20	20	50.0	50.0	59.0	58.1	8.0	0.0	1.059990	118672	119640	1208-1208	1.000000	71.6
ccsA-ndhD_p1	ccsA-ndhD	1	TGTGGCTTCTATGGGCTTTC	CAGTGGACAAAGTCGAAGCT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.257690	119641	119953	454-455	1.000000	77.9
ccsA-ndhD_p2	ccsA-ndhD	2	TGTGGCTTCTATGGGCTTTC	TCAGTGGACAAAGTCGAAGC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.262906	119641	119953	455-456	1.000000	77.9
ccsA-ndhD_p3	ccsA-ndhD	3	TTCCGCAATTGTGGCTTCTA	CAGTGGACAAAGTCGAAGCT	20	20	45.0	50.0	57.8	58.1	29.6	0.0	0.321583	119641	119953	463-464	1.000000	77.4
ccsA-ndhD_p4	ccsA-ndhD	4	TTCCGCAATTGTGGCTTCTA	TCAGTGGACAAAGTCGAAGC	20	20	45.0	50.0	57.8	58.1	29.6	0.0	0.326799	119641	119953	464-465	1.000000	77.4
ccsA-ndhD_p5	ccsA-ndhD	5	AATTCCGCAATTGTGGCTTC	CAGTGGACAAAGTCGAAGCT	20	20	45.0	50.0	57.6	58.1	29.6	0.0	0.497574	119641	119953	465-466	1.000000	76.0
ndhD_p1	ndhD	1	TGGACAACGAACAATTTTCA	GCACGGGTTTTTCTGGTCTA	20	20	35.0	50.0	54.0	58.1	0.0	0.0	4.158508	119954	121453	1603-1604	1.000000	46.8
ndhD_p2	ndhD	2	TGGACAACGAACAATTTTCA	ACCGACAAAAACCCGTACTC	20	20	35.0	50.0	54.0	58.1	0.0	0.0	4.175621	119954	121453	1663-1664	1.000000	46.6
ndhD_p3	ndhD	3	TGGACAACGAACAATTTTCA	CGACAAAAACCCGTACTCGA	20	20	35.0	50.0	54.0	58.2	0.0	0.0	4.275662	119954	121453	1661-1662	1.000000	45.8
ndhD_p4	ndhD	4	TGGACAACGAACAATTTTCAA	GCACGGGTTTTTCTGGTCTA	21	20	33.3	50.0	54.8	58.1	0.0	0.0	4.298110	119954	121453	1603-1604	1.000000	45.7
ndhD_p5	ndhD	5	TTGGACAACGAACAATTTTCA	GCACGGGTTTTTCTGGTCTA	21	20	33.3	50.0	54.8	58.1	0.0	0.0	4.298110	119954	121453	1604-1605	1.000000	45.7
rps15_p1	rps15	1	TCCTCGATTCATTCTTACATGA	AAGGGGAGAGAGAGGATTGC	22	20	36.4	55.0	54.7	58.5	0.0	0.0	5.760364	127664	127936	398-398	1.000000	40.0
rps15_p2	rps15	2	TCCTCGATTCATTCTTACATGA	GGAGAGAGAGGATTGCGTTT	22	20	36.4	50.0	54.7	57.3	0.0	0.0	5.948544	127664	127936	394-394	1.000000	40.0
rps15_p3	rps15	3	ACGAAAAGAAGTTCATCAAGATTCA	AAGGGGAGAGAGAGGATTGC	25	20	32.0	55.0	57.5	58.5	5.5	0.0	5.990504	127664	127936	366-366	1.000000	40.0
rps15_p4	rps15	4	TCCTCGATTCATTCTTACATGAA	AAGGGGAGAGAGAGGATTGC	23	20	34.8	55.0	55.5	58.5	0.0	0.0	5.995748	127664	127936	398-398	1.000000	40.0
rps15_p5	rps15	5	TTCCTCGATTCATTCTTACATGA	AAGGGGAGAGAGAGGATTGC	23	20	34.8	55.0	55.5	58.5	0.0	0.0	5.995748	127664	127936	399-399	1.000000	40.0
ycf1_p1	ycf1	1	ACCATAGTCCTTTTCTCGGT	TCTCCTGCTTCGACTGAGAA	20	20	45.0	50.0	55.9	58.1	0.0	0.0	2.229445	128786	128905	259-259	1.000000	62.5
ycf1_p2	ycf1	2	ACCATAGTCCTTTTCTCGGT	ATTGTCTCCTGCTTCGACTG	20	20	45.0	50.0	55.9	57.6	0.0	0.0	2.517310	128786	128905	263-263	1.000000	60.2
ycf1_p3	ycf1	3	ACCATAGTCCTTTTCTCGGT	TTGTCTCCTGCTTCGACTGA	20	20	45.0	50.0	55.9	58.7	0.0	0.0	2.812224	128786	128905	262-262	1.000000	57.8
ycf1_p4	ycf1	4	ACCATAGTCCTTTTCTCGGT	CTCCTGCTTCGACTGAGAAA	20	20	45.0	50.0	55.9	57.3	0.0	0.0	2.872914	128786	128905	258-258	1.000000	57.3
ycf1_p5	ycf1	5	ACCATAGTCCTTTTCTCGGT	GTCTCCTGCTTCGACTGAGA	20	20	45.0	55.0	55.9	58.8	0.0	0.0	2.970925	128786	128905	260-260	1.000000	56.5