primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score ycf1-trnN-GUU_p1 ycf1-trnN-GUU 1 TCAGGCATAACACTAGTAGA ACCACTGAGCTACTGAGGAA 20 20 40.0 50.0 52.5 57.7 3.6 11.2 5.822360 18574 19034 530-708 1.000000 47.5 ycf1-trnN-GUU_p2 ycf1-trnN-GUU 2 TTCAGGCATAACACTAGTAGA ACCACTGAGCTACTGAGGAA 21 20 38.1 50.0 53.4 57.7 3.6 11.2 5.872380 18574 19034 531-709 1.000000 47.5 ycf1-trnN-GUU_p3 ycf1-trnN-GUU 3 TTTCAGGCATAACACTAGTAGA ACCACTGAGCTACTGAGGAA 22 20 36.4 50.0 54.3 57.7 3.6 11.2 6.009143 18574 19034 532-710 1.000000 47.5 ycf1-trnN-GUU_p4 ycf1-trnN-GUU 4 TCAGGCATAACACTAGTAGAT ACCACTGAGCTACTGAGGAA 21 20 38.1 50.0 53.2 57.7 3.6 11.2 6.107885 18574 19034 530-708 1.000000 47.5 ycf1-trnN-GUU_p5 ycf1-trnN-GUU 5 GCTGAATTAACTATTTTCAGGCA ACCACTGAGCTACTGAGGAA 23 20 34.8 50.0 55.6 57.7 14.5 11.2 5.759782 18574 19034 546-724 1.000000 47.3 trnE-UUC-rrn16_p1 trnE-UUC-rrn16 1 TGAATCGCCCCATCTATCCT CCGCGAATCCTCTTGAAAGA 20 20 50.0 50.0 58.0 58.0 0.0 8.2 0.040172 25344 25649 1051-1064 1.000000 84.2 trnE-UUC-rrn16_p2 trnE-UUC-rrn16 2 ATTGTACTGCGCTCTCCAAG CCTGTCCTCAGTTCGGATTG 20 20 50.0 55.0 58.0 58.0 9.7 0.0 0.031472 25344 25649 1260-1268 1.000000 83.9 trnE-UUC-rrn16_p3 trnE-UUC-rrn16 3 TGCTTGTTCCACCTTCTTCC CCTGTCCTCAGTTCGGATTG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.034479 25344 25649 1238-1246 1.000000 83.9 trnE-UUC-rrn16_p4 trnE-UUC-rrn16 4 ATTGTACTGCGCTCTCCAAG CCGCGAATCCTCTTGAAAGA 20 20 50.0 50.0 58.0 58.0 9.7 8.2 0.025881 25344 25649 1550-1558 1.000000 83.2 trnE-UUC-rrn16_p5 trnE-UUC-rrn16 5 TGCTTGTTCCACCTTCTTCC CCGCGAATCCTCTTGAAAGA 20 20 50.0 50.0 58.0 58.0 0.0 8.2 0.028889 25344 25649 1528-1536 1.000000 83.2 trnL-CAA-ycf2_p1 trnL-CAA-ycf2 1 GGTGGTGGAATGGTAGACAC CCGGACGAGATGGTTGTTAC 20 20 55.0 55.0 57.9 58.4 0.0 0.0 0.472437 32646 33166 628-653 1.000000 82.3 trnL-CAA-ycf2_p2 trnL-CAA-ycf2 2 CCTTGGTGGTGGAATGGTAG CCGGACGAGATGGTTGTTAC 20 20 55.0 55.0 57.9 58.4 0.0 0.0 0.491782 32646 33166 632-657 1.000000 82.1 trnL-CAA-ycf2_p3 trnL-CAA-ycf2 3 GGTGGTGGAATGGTAGACAC AAAGATGGCTTTTCCCGGAC 20 20 55.0 50.0 57.9 58.5 0.0 0.7 0.563583 32646 33166 642-667 1.000000 81.5 trnL-CAA-ycf2_p4 trnL-CAA-ycf2 4 CCTTGGTGGTGGAATGGTAG AAAGATGGCTTTTCCCGGAC 20 20 55.0 50.0 57.9 58.5 0.0 0.7 0.582929 32646 33166 646-671 1.000000 81.3 trnL-CAA-ycf2_p5 trnL-CAA-ycf2 5 TGGTGGTGGAATGGTAGACA CCGGACGAGATGGTTGTTAC 20 20 50.0 55.0 58.3 58.4 0.0 0.0 0.627086 32646 33166 629-654 1.000000 81.1 trnM-CAU-rpl23_p1 trnM-CAU-rpl23 1 ATTGGCGAAGTCGTAGGTTC CGACCCAACGTTTTATTTCTGT 20 22 50.0 40.9 58.0 57.5 2.3 0.0 2.483273 39310 40035 865-881 1.000000 69.8 trnM-CAU-rpl23_p2 trnM-CAU-rpl23 2 GGCGAAGTCGTAGGTTCAAT CGACCCAACGTTTTATTTCTGT 20 22 50.0 40.9 58.0 57.5 2.3 0.0 2.483273 39310 40035 862-878 1.000000 69.8 trnM-CAU-rpl23_p3 trnM-CAU-rpl23 3 GAATGGTTAAAGCGCCCAAC CGACCCAACGTTTTATTTCTGT 20 22 50.0 40.9 58.0 57.5 2.3 0.0 2.486503 39310 40035 890-906 1.000000 69.6 trnM-CAU-rpl23_p4 trnM-CAU-rpl23 4 GGTAGTAGGATCCCCACGAA CGACCCAACGTTTTATTTCTGT 20 22 55.0 40.9 57.9 57.5 8.6 0.0 2.554990 39310 40035 922-938 1.000000 68.9 trnM-CAU-rpl23_p5 trnM-CAU-rpl23 5 GGCGAAGTCGTAGGTTCAAT TCGACCCAACGTTTTATTTCTG 20 22 50.0 40.9 58.0 57.3 2.3 0.0 2.741632 39310 40035 863-879 1.000000 67.8 rps19_p1 rps19 1 TGGAAAGGGTGGACAATTGG CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 58.0 58.0 10.8 0.0 0.023978 42172 42456 1562-1562 0.500000 59.8 rps19_p2 rps19 2 AACCCTGTAGACCATCCACA CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.054599 42172 42456 1330-1330 0.500000 59.6 rps19_p3 rps19 3 GGGTGGACAATTGGCTAGAG CAATTCCCTCGACACCAGAG 20 20 55.0 55.0 58.0 58.0 12.0 0.0 0.059859 42172 42456 1556-1556 0.500000 59.5 rps19_p4 rps19 4 GCGGTAGCGAAACTTATTGC CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 57.9 58.0 20.2 0.0 0.102670 42172 42456 1528-1528 0.500000 59.2 rps19_p5 rps19 5 GTTGGACAAGTCGGGAATGT CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.123526 42172 42456 1432-1432 0.500000 59.0 rps19-rpl22_p1 rps19-rpl22 1 AACCCTGTAGACCATCCACA AGTCTGAAACCAAGCGGATG 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.225350 42457 42778 1105-1184 1.000000 86.3 rps19-rpl22_p2 rps19-rpl22 2 AACCCTGTAGACCATCCACA ATCCATTCCACACGTGCAAT 20 20 50.0 45.0 58.0 58.2 0.0 17.2 0.200751 42457 42778 1606-1685 1.000000 85.5 rps19-rpl22_p3 rps19-rpl22 3 AACCCTGTAGACCATCCACA CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.054599 42457 42778 1330-1330 0.500000 59.6 rps19-rpl22_p4 rps19-rpl22 4 CCGCACTTGGAAGAAGAAGT CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.131501 42457 42778 1248-1248 0.500000 58.9 rps19-rpl22_p5 rps19-rpl22 5 AGGGAAGCGTCCTGTAGTAA CAATTCCCTCGACACCAGAG 20 20 50.0 55.0 57.8 58.0 18.5 0.0 0.242698 42457 42778 1364-1364 0.500000 58.1 rpl14-rps8_p1 rpl14-rps8 1 GTTTCATTAGCACCCGAGGT CAGCGATAGTGTCTTTACCCA 20 21 50.0 47.6 57.9 57.5 0.0 0.0 1.629714 45740 46257 570-603 1.000000 72.3 rpl14-rps8_p2 rpl14-rps8 2 GTTTCATTAGCACCCGAGGT AGCGATAGTGTCTTTACCCAC 20 21 50.0 47.6 57.9 57.5 0.0 0.0 1.631372 45740 46257 569-602 1.000000 72.3 rpl14-rps8_p3 rpl14-rps8 3 GTTTCATTAGCACCCGAGGT CGATAGTGTCTTTACCCACGA 20 21 50.0 47.6 57.9 57.2 0.0 0.0 1.879688 45740 46257 567-600 1.000000 70.3 rpl14-rps8_p4 rpl14-rps8 4 GTTTCATTAGCACCCGAGGT TCAGCGATAGTGTCTTTACCC 20 21 50.0 47.6 57.9 57.2 0.0 0.0 1.905047 45740 46257 571-604 1.000000 70.1 rpl14-rps8_p5 rpl14-rps8 5 GTTTCATTAGCACCCGAGGT GCGATAGTGTCTTTACCCACG 20 21 50.0 52.4 57.9 58.8 0.0 0.0 1.918443 45740 46257 568-601 1.000000 70.0 clpP_p1 clpP 1 AACAGGGTCAGAGGGATCAA CTCTAACCTTCCCAACCACG 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.139555 54975 56163 2539-2668 1.000000 84.4 clpP_p2 clpP 2 ACAGGGTCAGAGGGATCAAA CTCTAACCTTCCCAACCACG 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.139555 54975 56163 2538-2667 1.000000 84.4 clpP_p3 clpP 3 AACAGGGTCAGAGGGATCAA AGCGGAACCCTAACCTAAGT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.096402 54975 56163 1834-1834 0.500000 59.2 clpP_p4 clpP 4 ACAGGGTCAGAGGGATCAAA AGCGGAACCCTAACCTAAGT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.096402 54975 56163 1833-1833 0.500000 59.2 clpP_p5 clpP 5 GGTGGTGGGGGTTTACTTTT AGCGGAACCCTAACCTAAGT 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.142707 54975 56163 1473-1473 0.500000 58.9 clpP-rps12_p1 clpP-rps12 1 GCTTCGTGGCAGATATCCAA CTCTAACCTTCCCAACCACG 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.141203 56164 56239 843-843 0.500000 58.9 clpP-rps12_p2 clpP-rps12 2 GCTTCGTGGCAGATATCCAA GATTCGGTTTGCACCAATCG 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.151390 56164 56239 374-374 0.500000 58.8 clpP-rps12_p3 clpP-rps12 3 GCTTCGTGGCAGATATCCAA CTAGTACATGTTCCTCGGCG 20 20 50.0 55.0 58.0 57.9 0.0 2.3 0.167557 56164 56239 279-279 0.500000 58.7 clpP-rps12_p4 clpP-rps12 4 GCTTCGTGGCAGATATCCAA AGTCGCACATACACCCTAGT 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.197789 56164 56239 294-294 0.500000 58.4 clpP-rps12_p5 clpP-rps12 5 GCTTCGTGGCAGATATCCAA AGTTCGGATTGGCTGTCTTG 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.232928 56164 56239 223-223 0.500000 58.1 psaJ-trnP-UGG_p1 psaJ-trnP-UGG 1 AATGTCAATGCATCCGGGAA AAAACAAACGCGCTACCAAG 20 20 45.0 45.0 57.9 57.9 0.0 5.7 0.256267 59084 59660 728-733 1.000000 82.7 psaJ-trnP-UGG_p2 psaJ-trnP-UGG 2 AATGTCAATGCATCCGGGAA CAAAACAAACGCGCTACCAA 20 20 45.0 45.0 57.9 58.2 0.0 5.7 0.313525 59084 59660 729-734 1.000000 82.2 psaJ-trnP-UGG_p3 psaJ-trnP-UGG 3 AATGTCAATGCATCCGGGAA TTGTACCCAAAACAAACGCG 20 20 45.0 45.0 57.9 57.8 0.0 3.5 0.332681 59084 59660 736-741 1.000000 82.0 psaJ-trnP-UGG_p4 psaJ-trnP-UGG 4 GAATGTCAATGCATCCGGGA AAAACAAACGCGCTACCAAG 20 20 50.0 45.0 58.3 57.9 0.0 5.7 0.434771 59084 59660 729-734 1.000000 81.2 psaJ-trnP-UGG_p5 psaJ-trnP-UGG 5 ATGTCAATGCATCCGGGAAT AAAACAAACGCGCTACCAAG 20 20 45.0 45.0 57.6 57.9 0.0 5.7 0.483894 59084 59660 727-732 1.000000 80.8 trnQ-UUG-psbK_p1 trnQ-UUG-psbK 1 GACCAAAACCCGTTGCCTTA AGGCTTCAGGTAATTTCGCC 20 20 50.0 50.0 58.7 58.2 0.0 0.0 0.928056 69616 70316 781-827 1.000000 80.1 trnQ-UUG-psbK_p2 trnQ-UUG-psbK 2 CGAATAACGGGACCAAAACC AGGCTTCAGGTAATTTCGCC 20 20 50.0 50.0 57.1 58.2 0.0 0.0 1.154522 69616 70316 791-837 1.000000 78.2 trnQ-UUG-psbK_p3 trnQ-UUG-psbK 3 AAAACCCGTTGCCTTACCAC AGGCTTCAGGTAATTTCGCC 20 20 50.0 50.0 59.0 58.2 0.0 0.0 1.214207 69616 70316 777-823 1.000000 77.8 trnQ-UUG-psbK_p4 trnQ-UUG-psbK 4 ACCAAAACCCGTTGCCTTAC AGGCTTCAGGTAATTTCGCC 20 20 50.0 50.0 59.0 58.2 0.0 0.0 1.214207 69616 70316 780-826 1.000000 77.8 trnQ-UUG-psbK_p5 trnQ-UUG-psbK 5 CAAAACCCGTTGCCTTACCA AGGCTTCAGGTAATTTCGCC 20 20 50.0 50.0 59.0 58.2 0.0 0.0 1.214988 69616 70316 778-824 1.000000 77.8 trnC-GCA-petN_p1 trnC-GCA-petN 1 CGATATGGCCGAGTGGTAAG TTAAAGCAGCCCAAGCAAGA 20 20 55.0 45.0 57.9 58.0 0.0 0.0 0.072037 91513 92489 1080-1178 1.000000 84.4 trnC-GCA-petN_p2 trnC-GCA-petN 2 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.088172 91513 92489 1040-1138 1.000000 84.4 trnC-GCA-petN_p3 trnC-GCA-petN 3 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.273256 91513 92489 1034-1132 1.000000 83.0 trnC-GCA-petN_p4 trnC-GCA-petN 4 CGATATGGCCGAGTGGTAAG CAGCCCAAGCAAGACTTACT 20 20 55.0 50.0 57.9 57.8 0.0 0.0 0.257120 91513 92489 1074-1172 1.000000 82.9 trnC-GCA-petN_p5 trnC-GCA-petN 5 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.4 58.0 14.7 0.0 0.361535 91513 92489 1058-1156 1.000000 82.2 psbC-trnS-UGA_p1 psbC-trnS-UGA 1 TCGAGATTCTGAGCCTGTTC GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 57.1 58.0 0.0 0.0 0.970807 99345 99559 336-350 1.000000 79.5 psbC-trnS-UGA_p2 psbC-trnS-UGA 2 TCGAGATTCTGAGCCTGTTC GGTTGATAGCTCCGGTCTTG 20 20 50.0 55.0 57.1 58.1 0.0 0.0 1.008126 99345 99559 321-335 1.000000 79.5 psbC-trnS-UGA_p3 psbC-trnS-UGA 3 CGAGATTCTGAGCCTGTTCT GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 57.0 58.0 0.0 0.0 0.978846 99345 99559 335-349 1.000000 79.4 psbC-trnS-UGA_p4 psbC-trnS-UGA 4 TCGAGATTCTGAGCCTGTTC GCTGAGTGGTTGATAGCTCC 20 20 50.0 55.0 57.1 58.1 0.0 0.0 1.002706 99345 99559 328-342 1.000000 79.4 psbC-trnS-UGA_p5 psbC-trnS-UGA 5 TCGAGATTCTGAGCCTGTTC GGCTGAGTGGTTGATAGCTC 20 20 50.0 55.0 57.1 58.1 0.0 0.0 1.002706 99345 99559 329-343 1.000000 79.4 trnG-GCC-trnM-CAU_p1 trnG-GCC-trnM-CAU 1 GTTCGATTCCCGCTATCCG CTTGAGGTCACGGGTTCAAA 19 20 57.9 50.0 58.2 58.0 0.0 4.2 1.227721 100683 100738 114-147 1.000000 73.9 trnG-GCC-trnM-CAU_p2 trnG-GCC-trnM-CAU 2 GGGTTCGATTCCCGCTATC CTTGAGGTCACGGGTTCAAA 19 20 57.9 50.0 57.8 58.0 9.9 4.2 1.250656 100683 100738 116-149 1.000000 73.6 trnG-GCC-trnM-CAU_p3 trnG-GCC-trnM-CAU 3 GGTTCGATTCCCGCTATCC CTTGAGGTCACGGGTTCAAA 19 20 57.9 50.0 57.8 58.0 0.0 4.2 1.250656 100683 100738 115-148 1.000000 73.6 trnG-GCC-trnM-CAU_p4 trnG-GCC-trnM-CAU 4 GTTCGATTCCCGCTATCCG TGAGGTCACGGGTTCAAATC 19 20 57.9 50.0 58.2 57.8 0.0 0.0 1.368426 100683 100738 112-145 1.000000 72.8 trnG-GCC-trnM-CAU_p5 trnG-GCC-trnM-CAU 5 GGTTCGATTCCCGCTATCC TGAGGTCACGGGTTCAAATC 19 20 57.9 50.0 57.8 57.8 0.0 0.0 1.391362 100683 100738 113-146 1.000000 72.6 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 110139 111156 1026-1069 1.000000 87.6 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 110139 111156 1034-1077 1.000000 87.1 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 110139 111156 1058-1101 1.000000 86.6 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 110139 111156 1066-1109 1.000000 86.1 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 110139 111156 1040-1083 1.000000 85.7 trnL-UAA-trnF-GAA_p1 trnL-UAA-trnF-GAA 1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.5 58.1 0.0 20.1 0.526305 111770 111902 223-226 1.000000 80.1 trnL-UAA-trnF-GAA_p2 trnL-UAA-trnF-GAA 2 AATCGTGAGGGTTCAAGTCC CAAATGAATTGCCGGGAACC 20 20 50.0 50.0 57.5 58.0 0.0 11.9 0.483162 111770 111902 246-249 1.000000 80.0 trnL-UAA-trnF-GAA_p3 trnL-UAA-trnF-GAA 3 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 20 20 50.0 50.0 57.5 57.5 0.0 20.1 0.931776 111770 111902 220-223 1.000000 76.9 trnL-UAA-trnF-GAA_p4 trnL-UAA-trnF-GAA 4 AATCGTGAGGGTTCAAGTCC TTCAGTCCTCTGCTCTACCG 20 20 50.0 55.0 57.5 58.5 0.0 0.0 1.009676 111770 111902 200-203 1.000000 76.7 trnL-UAA-trnF-GAA_p5 trnL-UAA-trnF-GAA 5 TTCAAGTCCCTCTATCCCCA CAAATGAATTGCCGGGAACC 20 20 50.0 50.0 57.0 58.0 0.0 11.9 0.970288 111770 111902 235-238 1.000000 76.3 ndhC-trnI-AAU_p1 ndhC-trnI-AAU 1 GCCCTTTTCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.7 57.8 0.0 0.0 0.530971 114345 114760 561-568 1.000000 79.9 ndhC-trnI-AAU_p2 ndhC-trnI-AAU 2 GCCCTTTTCTAATCGGAGCT GCACGTTGGGTCTTTCAAAC 20 20 50.0 50.0 57.7 58.2 0.0 0.0 0.498568 114345 114760 624-631 1.000000 79.7 ndhC-trnI-AAU_p3 ndhC-trnI-AAU 3 GCCCTTTTCTAATCGGAGCT AATGCACGTTGGGTCTTTCA 20 20 50.0 45.0 57.7 58.3 0.0 0.0 0.647334 114345 114760 627-634 1.000000 78.5 ndhC-trnI-AAU_p4 ndhC-trnI-AAU 4 GCCCTTTTCTAATCGGAGCT ATGCACGTTGGGTCTTTCAA 20 20 50.0 45.0 57.7 58.3 0.0 0.0 0.647334 114345 114760 626-633 1.000000 78.5 ndhC-trnI-AAU_p5 ndhC-trnI-AAU 5 GGCCCTTTTCTAATCGGAGC GCACGTTGGGTCTTTCAAAC 20 20 55.0 50.0 58.7 58.2 0.0 0.0 0.854929 114345 114760 625-632 1.000000 76.9