primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
accB_p1	accB	1	TCTTACCGACACCTGGTCTT	AATCGTGAGCATCTGCATGT	20	20	50.0	45.0	58.0	58.0	0.0	4.8	0.039475	6292	6843	1606-1606	0.500000	59.7
accB_p2	accB	2	TCTTACCGACACCTGGTCTT	AATCCTCCACCAACATCAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.140159	6292	6843	1835-1835	0.500000	58.9
accB_p3	accB	3	TCTTACCGACACCTGGTCTT	CCTCCACCAACATCAGCATT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.162002	6292	6843	1832-1832	0.500000	58.7
accB_p4	accB	4	TCTTACCGACACCTGGTCTT	TGATTCCTGAGGCTCTCCAT	20	20	50.0	50.0	58.0	57.8	0.0	16.7	0.192609	6292	6843	1891-1891	0.500000	58.5
accB_p5	accB	5	TCTTACCGACACCTGGTCTT	CGTGAGCATCTGCATGTAGA	20	20	50.0	50.0	58.0	57.8	0.0	16.2	0.219757	6292	6843	1603-1603	0.500000	58.2
Gsul_orf1_p1	Gsul_orf1	1	ACAATGGGTATTGGGAGGGA	CTACCAGTTTCGCCACCTAC	20	20	50.0	55.0	58.0	58.0	5.2	0.0	0.011572	49304	50065	1563-1563	0.500000	59.9
Gsul_orf1_p2	Gsul_orf1	2	ACAATGGGTATTGGGAGGGA	TGATGCGTCTACCAGTTTCG	20	20	50.0	50.0	58.0	58.0	5.2	7.6	0.014802	49304	50065	1571-1571	0.500000	59.9
Gsul_orf1_p3	Gsul_orf1	3	ACAATGGGTATTGGGAGGGA	TCTACCAGTTTCGCCACCTA	20	20	50.0	50.0	58.0	58.1	5.2	0.0	0.078114	49304	50065	1564-1564	0.500000	59.4
Gsul_orf1_p4	Gsul_orf1	4	ACAATGGGTATTGGGAGGGA	GTGTGATGCGTCTACCAGTT	20	20	50.0	50.0	58.0	57.9	5.2	7.6	0.085887	49304	50065	1574-1574	0.500000	59.3
Gsul_orf1_p5	Gsul_orf1	5	ACAATGGGTATTGGGAGGGA	GAGTGTGATGCGTCTACCAG	20	20	50.0	55.0	58.0	58.1	5.2	7.6	0.090968	49304	50065	1576-1576	0.500000	59.3
ilvH_p1	ilvH	1	GTGCTACTGGTGGTTCTGTT	GGTATGGCCAAGTGGTAAGG	20	20	50.0	55.0	57.7	57.9	0.0	11.0	0.308900	67717	68271	1066-1066	0.500000	57.5
ilvH_p2	ilvH	2	GTGCTACTGGTGGTTCTGTT	GGTAAGGCAGAGGATTGCAA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.384888	67717	68271	1053-1053	0.500000	56.9
ilvH_p3	ilvH	3	TACGTGCTACTGGTGGTTCT	GGTATGGCCAAGTGGTAAGG	20	20	50.0	55.0	58.4	57.9	0.0	11.0	0.428446	67717	68271	1069-1069	0.500000	56.6
ilvH_p4	ilvH	4	GTGCTACTGGTGGTTCTGTT	AAGGGGATGTGTTGGAATGG	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.492606	67717	68271	984-984	0.500000	56.1
ilvH_p5	ilvH	5	GTGCTACTGGTGGTTCTGTT	AAGTCTTAAAGCGCGCAATG	20	20	50.0	45.0	57.7	57.8	0.0	35.1	0.503428	67717	68271	1661-1661	0.500000	56.0
ycf80_p1	ycf80	1	AGTCGGCAGTGCAACATTTA	ACCACCACACCTATCGGTAA	20	20	45.0	50.0	58.1	58.1	18.6	0.0	0.167747	72480	74258	2345-2345	0.500000	58.7
ycf80_p2	ycf80	2	AGTCGGCAGTGCAACATTTA	ACTATACCAGCTGCTAGGCA	20	20	45.0	50.0	58.1	57.9	18.6	8.7	0.197228	72480	74258	2518-2518	0.500000	58.4
ycf80_p3	ycf80	3	GCATAAAACAGGGAGGCTGT	ACCACCACACCTATCGGTAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.219199	72480	74258	2276-2276	0.500000	58.2
ycf80_p4	ycf80	4	TAAAACAGGGAGGCTGTTCG	ACCACCACACCTATCGGTAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.237535	72480	74258	2273-2273	0.500000	58.1
ycf80_p5	ycf80	5	GCATAAAACAGGGAGGCTGT	ACTATACCAGCTGCTAGGCA	20	20	50.0	50.0	58.2	57.9	0.0	8.7	0.248681	72480	74258	2449-2449	0.500000	58.0
chlI_p1	chlI	1	GCAGATGGTCACTGGTTCAA	GCAGAAGGCAATCCAGCTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.125326	76077	77117	1739-1739	0.500000	59.0
chlI_p2	chlI	2	GCAGATGGTCACTGGTTCAA	TTCCAGCCGTTTTAGCACTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.128121	76077	77117	1326-1326	0.500000	59.0
chlI_p3	chlI	3	GCCTTACAAGCAGATGGTCA	GCAGAAGGCAATCCAGCTAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.135406	76077	77117	1748-1748	0.500000	58.9
chlI_p4	chlI	4	TGCCTTACAAGCAGATGGTC	GCAGAAGGCAATCCAGCTAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.135406	76077	77117	1749-1749	0.500000	58.9
chlI_p5	chlI	5	GCCTTACAAGCAGATGGTCA	TTCCAGCCGTTTTAGCACTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.138201	76077	77117	1335-1335	0.500000	58.9
carA_p1	carA	1	ACGAGATTCAAACTCAAAACGC	AGCTCCTATAGGTCATTTGCC	22	21	40.9	47.6	58.1	57.2	0.0	18.5	3.905425	84007	85206	1549-1549	0.500000	28.8
carA_p2	carA	2	ACGAGATTCAAACTCAAAACGC	GCTCCTATAGGTCATTTGCCT	22	21	40.9	47.6	58.1	57.2	0.0	18.5	3.905425	84007	85206	1548-1548	0.500000	28.8
carA_p3	carA	3	CGAGATTCAAACTCAAAACGCT	AGCTCCTATAGGTCATTTGCC	22	21	40.9	47.6	57.9	57.2	0.0	18.5	3.912810	84007	85206	1548-1548	0.500000	28.7
carA_p4	carA	4	CGAGATTCAAACTCAAAACGCT	GCTCCTATAGGTCATTTGCCT	22	21	40.9	47.6	57.9	57.2	0.0	18.5	3.912810	84007	85206	1547-1547	0.500000	28.7
carA_p5	carA	5	ACGAGATTCAAACTCAAAACGC	GCTCCTATAGGTCATTTGCCTT	22	22	40.9	45.5	58.1	57.9	0.0	18.5	4.218587	84007	85206	1548-1548	0.500000	26.3
Gsul_orf2_p1	Gsul_orf2	1	CAATACCATCCTGAAGGGGC	TTTTCCTGGTCCTGCTCCTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.051981	85743	86537	1558-1558	0.500000	59.6
Gsul_orf2_p2	Gsul_orf2	2	CAATACCATCCTGAAGGGGC	GTGGGTCTCCGCTTTTTAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.193550	85743	86537	1802-1802	0.500000	58.5
Gsul_orf2_p3	Gsul_orf2	3	CAATACCATCCTGAAGGGGC	TTCCTGGTCCTGCTCCTATT	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.283552	85743	86537	1556-1556	0.500000	57.7
Gsul_orf2_p4	Gsul_orf2	4	CAATACCATCCTGAAGGGGC	TTTCCTGGTCCTGCTCCTAT	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.283552	85743	86537	1557-1557	0.500000	57.7
Gsul_orf2_p5	Gsul_orf2	5	CAGGGCCTCATGACAATCAT	TTTTCCTGGTCCTGCTCCTA	20	20	50.0	50.0	57.6	58.0	1.0	0.0	0.398844	85743	86537	1536-1536	0.500000	56.8
cobA_p1	cobA	1	AGCCACCAAGACTCCAATTG	TGCGGGTTCTTTTGGAATCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.096566	86597	87379	1396-1396	0.500000	59.2
cobA_p2	cobA	2	AGCCACCAAGACTCCAATTG	TCACTTATGGCCATCAGTGC	20	20	50.0	50.0	58.1	58.0	0.0	38.6	0.127545	86597	87379	1684-1684	0.500000	59.0
cobA_p3	cobA	3	AGCCACCAAGACTCCAATTG	TGTTGGTTTTGTCCTGCCTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.225557	86597	87379	1848-1848	0.500000	58.2
cobA_p4	cobA	4	AGCCACCAAGACTCCAATTG	TTGTTGGTTTTGTCCTGCCT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.225557	86597	87379	1849-1849	0.500000	58.2
cobA_p5	cobA	5	AGTTAGCCACCAAGACTCCA	TGCGGGTTCTTTTGGAATCA	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.282120	86597	87379	1400-1400	0.500000	57.7
cysT_p1	cysT	1	AGACTGCACCAAATTCACCA	CCACTCGAAAAATCGCATGG	20	20	45.0	50.0	57.6	57.8	0.0	0.0	0.541780	89424	90239	1997-1997	0.500000	55.7
cysT_p2	cysT	2	AGACTGCACCAAATTCACCA	AAAATCGCATGGAGTGTTGC	20	20	45.0	45.0	57.6	57.6	0.0	0.0	0.715809	89424	90239	1989-1989	0.500000	54.3
cysT_p3	cysT	3	AACTTGCCAAGAGTTTGCAC	CCACTCGAAAAATCGCATGG	20	20	45.0	50.0	57.4	57.8	12.3	0.0	0.762966	89424	90239	1903-1903	0.500000	53.9
cysT_p4	cysT	4	AGACTGCACCAAATTCACCA	ATGGAGTGTTGCCAATGGAC	20	20	45.0	50.0	57.6	58.5	0.0	2.1	0.814103	89424	90239	1981-1981	0.500000	53.5
cysT_p5	cysT	5	AGACTGCACCAAATTCACCA	CATGGAGTGTTGCCAATGGA	20	20	45.0	50.0	57.6	58.5	0.0	2.1	0.815225	89424	90239	1982-1982	0.500000	53.5
ycf23_p1	ycf23	1	AGCGTGTCCTTGTGATGTTT	TAGGAGTTTTCCCTCGCTCA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.099660	104691	105494	1597-1597	0.500000	59.2
ycf23_p2	ycf23	2	AGCGTGTCCTTGTGATGTTT	CCAAGCACCAGCAGTATCAA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.207591	104691	105494	1725-1725	0.500000	58.3
ycf23_p3	ycf23	3	TGTAAGAGCGTGTCCTTGTG	TAGGAGTTTTCCCTCGCTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.215894	104691	105494	1603-1603	0.500000	58.3
ycf23_p4	ycf23	4	TGTGTAAGAGCGTGTCCTTG	TAGGAGTTTTCCCTCGCTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.215894	104691	105494	1605-1605	0.500000	58.3
ycf23_p5	ycf23	5	GTGTAAGAGCGTGTCCTTGT	TAGGAGTTTTCCCTCGCTCA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.217376	104691	105494	1604-1604	0.500000	58.3
accA_p1	accA	1	TGCCCACTTTAATTGCCACT	GGGCTCATATTGGAGTTGGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.021360	105533	106564	1884-1884	0.500000	59.8
accA_p2	accA	2	TGCCCACTTTAATTGCCACT	GGGGCTCATATTGGAGTTGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.021360	105533	106564	1885-1885	0.500000	59.8
accA_p3	accA	3	TGCCCACTTTAATTGCCACT	GGAGCTGTATGGGCAAATGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.065380	105533	106564	1910-1910	0.500000	59.5
accA_p4	accA	4	TGCCCACTTTAATTGCCACT	AGCTGTATGGGCAAATGAGG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.071213	105533	106564	1908-1908	0.500000	59.4
accA_p5	accA	5	TGCCCACTTTAATTGCCACT	AGGAGCTGTATGGGCAAATG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.071213	105533	106564	1911-1911	0.500000	59.4
ccsA_p1	ccsA	1	ATAGGAGAAGGGGGTTCTGG	ACACGTTGATTTTGCGTTGA	20	20	55.0	40.0	57.8	57.2	0.0	0.0	1.032908	106902	107813	2048-2052	1.000000	83.4
ccsA_p2	ccsA	2	ATAGGAGAAGGGGGTTCTGG	TCAACACGTTGATTTTGCGT	20	20	55.0	40.0	57.8	57.2	0.0	8.5	1.032908	106902	107813	2051-2051	0.500000	51.7
ccsA_p3	ccsA	3	ATAGGAGAAGGGGGTTCTGG	GCGGTAAGCTGCCCAAATTA	20	20	55.0	50.0	57.8	58.9	0.0	0.0	1.098645	106902	107813	2154-2154	0.500000	51.2
ccsA_p4	ccsA	4	ATAGGAGAAGGGGGTTCTGG	CACGTTGATTTTGCGTTGAGA	20	21	55.0	42.9	57.8	58.0	0.0	0.0	1.217747	106902	107813	2047-2047	0.500000	50.3
ccsA_p5	ccsA	5	TTCTCCAGAAGCTTGCTCAG	ACACGTTGATTTTGCGTTGA	20	20	50.0	40.0	57.5	57.2	2.1	0.0	1.303848	106902	107813	1962-1962	0.500000	49.6
thiG_p1	thiG	1	TCCAAGGCAAATCCCCAAAA	CCTACACCTCCTTTTCCGGA	20	20	45.0	55.0	57.9	58.7	0.0	0.0	0.836422	113349	114185	1246-1246	0.500000	53.3
thiG_p2	thiG	2	GTCTACCTGGACCTGGTGAT	CCTACACCTCCTTTTCCGGA	20	20	55.0	55.0	58.1	58.7	28.5	0.0	0.859830	113349	114185	1169-1169	0.500000	53.1
thiG_p3	thiG	3	TGATGTCCAAGGCAAATCCC	CCTACACCTCCTTTTCCGGA	20	20	50.0	55.0	58.1	58.7	0.0	0.0	0.875889	113349	114185	1251-1251	0.500000	53.0
thiG_p4	thiG	4	TCCAAGGCAAATCCCCAAAA	ACACCTCCTTTTCCGGAAGT	20	20	45.0	50.0	57.9	58.9	0.0	18.0	0.971532	113349	114185	1243-1243	0.500000	52.2
thiG_p5	thiG	5	GTCTACCTGGACCTGGTGAT	ACACCTCCTTTTCCGGAAGT	20	20	55.0	50.0	58.1	58.9	28.5	18.0	0.994940	113349	114185	1166-1166	0.500000	52.0
minD_p1	minD	1	CTTGGACACCCTATTCCTGC	ACGTCGACCCTTAACAGTATG	20	21	55.0	47.6	58.0	57.5	0.0	10.6	1.543049	114269	115069	2229-2229	0.500000	47.7
minD_p2	minD	2	TAGCTGCTTCACTTGGACAC	ACGTCGACCCTTAACAGTATG	20	21	50.0	47.6	57.8	57.5	7.8	10.6	1.668759	114269	115069	2240-2240	0.500000	46.6
minD_p3	minD	3	TTCCATGGCTTTAGCTGCTT	ACGTCGACCCTTAACAGTATG	20	21	45.0	47.6	57.8	57.5	7.8	10.6	1.719579	114269	115069	2251-2251	0.500000	46.2
minD_p4	minD	4	TCCATGGCTTTAGCTGCTTC	ACGTCGACCCTTAACAGTATG	20	21	50.0	47.6	58.2	57.5	7.8	10.6	1.743747	114269	115069	2250-2250	0.500000	46.1
minD_p5	minD	5	GGACACCCTATTCCTGCATC	ACGTCGACCCTTAACAGTATG	20	21	55.0	47.6	57.7	57.5	0.0	10.6	1.761350	114269	115069	2226-2226	0.500000	45.9
ycf60_p1	ycf60	1	TTGCAAGACGTTTGGATGGT	GGGGAAGCTCTTTGGGAAAA	20	20	45.0	50.0	58.3	58.0	0.0	0.0	0.323327	115645	116583	2062-2062	0.500000	57.4
ycf60_p2	ycf60	2	TTGCAAGACGTTTGGATGGT	ACACCTTGGTGCTTATAGCG	20	20	45.0	50.0	58.3	58.0	0.0	18.7	0.337821	115645	116583	2148-2148	0.500000	57.3
ycf60_p3	ycf60	3	TTGCAAGACGTTTGGATGGT	CACCTTGGTGCTTATAGCGT	20	20	45.0	50.0	58.3	58.0	0.0	6.9	0.337821	115645	116583	2147-2147	0.500000	57.3
ycf60_p4	ycf60	4	TTGCAAGACGTTTGGATGGT	GAAGCCCACCTCAAGATGAG	20	20	45.0	55.0	58.3	58.0	0.0	0.3	0.349175	115645	116583	2362-2362	0.500000	57.2
ycf60_p5	ycf60	5	TTGCAAGACGTTTGGATGGT	TTTCGAACTCGGCTGTGAAA	20	20	45.0	45.0	58.3	58.1	0.0	0.0	0.370968	115645	116583	2106-2106	0.500000	57.0