primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CTCGTCCAACGGATTAGCAA	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	58.0	57.3	0.0	0.0	2.723441	8636	9950	1253-1428	1.000000	63.5
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AACTCGTCCAACGGATTAGC	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	58.0	57.3	0.0	0.0	2.724828	8636	9950	1255-1430	1.000000	63.5
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	20	22	45.0	40.9	58.0	57.3	0.0	0.0	2.757064	8636	9950	1241-1416	1.000000	63.3
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CTCGTCCAACGGATTAGCAA	TCGAACGAATCACACTTTTACC	20	22	50.0	40.9	58.0	57.0	0.0	0.0	2.982324	8636	9950	1254-1429	1.000000	61.5
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AACTCGTCCAACGGATTAGC	TCGAACGAATCACACTTTTACC	20	22	50.0	40.9	58.0	57.0	0.0	0.0	2.983712	8636	9950	1256-1431	1.000000	61.4
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	AGCGCTTTTATTTGCAAATCCT	20	22	50.0	36.4	57.9	57.8	0.0	27.3	2.310742	14067	14556	588-591	1.000000	64.4
atpF-atpH_p2	atpF-atpH	2	CAGTGGCCTAAGGAAACGAA	AGCGCTTTTATTTGCAAATCCT	20	22	50.0	36.4	57.8	57.8	3.3	27.3	2.371341	14067	14556	560-563	1.000000	64.0
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	GCATTAGCGCTTTTATTTGCA	20	21	50.0	38.1	57.9	56.5	0.0	18.7	2.651862	14067	14556	593-596	1.000000	61.6
atpF-atpH_p4	atpF-atpH	4	CAGTGGCCTAAGGAAACGAA	GCATTAGCGCTTTTATTTGCA	20	21	50.0	38.1	57.8	56.5	3.3	18.7	2.712461	14067	14556	565-568	1.000000	61.3
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	GCGCTTTTATTTGCAAATCCT	20	21	50.0	38.1	57.9	56.4	0.0	27.3	2.749876	14067	14556	587-590	1.000000	60.9
rps2-rpoC2_p1	rps2-rpoC2	1	GAGCCCTTATTGCAGCTGAT	GAAGGTTGGAACGAGCGTAT	20	20	50.0	50.0	58.0	58.0	20.4	0.0	0.027516	16979	17143	1099-1104	1.000000	80.9
rps2-rpoC2_p2	rps2-rpoC2	2	GAGCCCTTATTGCAGCTGAT	TTATCGATCCCAGGGGGTAC	20	20	50.0	55.0	58.0	58.0	20.4	5.3	0.041353	16979	17143	983-988	1.000000	80.9
rps2-rpoC2_p3	rps2-rpoC2	3	AACATAATGACACCGAGCCC	GAAGGTTGGAACGAGCGTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044357	16979	17143	1113-1118	1.000000	80.8
rps2-rpoC2_p4	rps2-rpoC2	4	GACATACCACCGAGCCATTT	GAAGGTTGGAACGAGCGTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044357	16979	17143	1139-1144	1.000000	80.8
rps2-rpoC2_p5	rps2-rpoC2	5	GCGAGATCTGGGTCACAATT	GAAGGTTGGAACGAGCGTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035809	16979	17143	1424-1429	1.000000	80.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCGGGTATTGTGGACATTCC	TAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.3	57.5	0.0	14.6	0.849553	27523	28749	1294-1312	1.000000	75.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGGGTATTGTGGACATTCCC	TAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.3	57.5	0.0	14.6	0.849553	27523	28749	1293-1311	1.000000	75.9
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	57.5	0.0	14.6	0.934514	27523	28749	1277-1295	1.000000	75.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.6	57.5	0.0	14.6	0.960477	27523	28749	1278-1296	1.000000	75.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GCCAAGCCCTGATCAATGAA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.5	57.5	0.0	14.6	1.047552	27523	28749	1348-1366	1.000000	74.2
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.413468	29978	30938	1113-1116	1.000000	69.7
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	29978	30938	1062-1065	1.000000	68.8
petN-psbM_p3	petN-psbM	3	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	29978	30938	1061-1064	1.000000	68.8
petN-psbM_p4	petN-psbM	4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	57.8	57.6	0.0	0.0	1.583671	29978	30938	1060-1063	1.000000	68.4
petN-psbM_p5	petN-psbM	5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.598551	29978	30938	1119-1122	1.000000	68.2
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGTGGCTGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.190415	36679	36805	235-240	1.000000	82.5
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	36679	36805	224-229	1.000000	82.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	CCTGTTCTTTCCATGACCCC	GCTGAGTGGTTGATAGCTCC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.213831	36679	36805	231-236	1.000000	82.4
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.213831	36679	36805	232-237	1.000000	82.4
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TTCCATGACCCCTCTTAGCT	GGTGGCTGAGTGGTTGATAG	20	20	50.0	55.0	57.7	58.0	0.0	0.0	0.300272	36679	36805	227-232	1.000000	81.8
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	TGGTTGAAGATAACGAGGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.099345	43124	43768	752-754	1.000000	80.5
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	GGTTGAAGATAACGAGGCGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.100733	43124	43768	751-753	1.000000	80.5
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	57.9	58.0	0.0	1.4	0.110498	43124	43768	822-824	1.000000	80.3
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	20	20	50.0	50.0	57.9	57.9	0.0	12.9	0.214563	43124	43768	820-822	1.000000	79.5
psaA-ycf3_p5	psaA-ycf3	5	GGCCCTTAGCTATTGTTCGT	TGGTTGAAGATAACGAGGCG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.331782	43124	43768	791-793	1.000000	78.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCCATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.283643	47720	48692	927-1035	1.000000	83.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47720	48692	939-1047	1.000000	82.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCCATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.391397	47720	48692	959-1067	1.000000	82.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	20	20	55.0	50.0	57.9	57.7	0.0	0.0	0.412322	47720	48692	936-1044	1.000000	81.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	20	20	50.0	50.0	57.8	57.8	15.5	0.0	0.465562	47720	48692	971-1079	1.000000	81.4
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AGAGAGAGTCCCATTCTGCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.8	58.1	0.0	20.1	0.296381	49290	49653	530-531	1.000000	79.1
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AGAGAGAGTCCCATTCTGCA	ATTCACGTGTCAGGAACCAG	20	20	50.0	50.0	57.8	57.8	0.0	16.5	0.414318	49290	49653	551-552	1.000000	78.1
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AGAGAGAGTCCCATTCTGCA	CACGTGTCAGGAACCAGATT	20	20	50.0	50.0	57.8	57.8	0.0	7.6	0.414318	49290	49653	548-549	1.000000	78.1
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GAGTCCCATTCTGCATGTCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.6	58.1	0.0	20.1	0.460167	49290	49653	525-526	1.000000	77.8
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	49290	49653	454-455	1.000000	77.5
atpB-rbcL_p1	atpB-rbcL	1	TGGGCCAATAATTTGAGCGA	GTCTCCGTTTGTGGTGACAT	20	20	45.0	50.0	57.9	58.1	2.1	9.4	0.257345	56371	57310	1018-1047	1.000000	81.7
atpB-rbcL_p2	atpB-rbcL	2	CTGGGCCAATAATTTGAGCG	GTCTCCGTTTGTGGTGACAT	20	20	50.0	50.0	57.5	58.1	8.0	9.4	0.618154	56371	57310	1019-1048	1.000000	78.8
atpB-rbcL_p3	atpB-rbcL	3	CGGGGGAAAAACAACATCCA	GTCTCCGTTTGTGGTGACAT	20	20	50.0	50.0	58.7	58.1	0.0	9.4	0.787870	56371	57310	1042-1071	1.000000	77.4
atpB-rbcL_p4	atpB-rbcL	4	CGCAGGACCAGAAGTAGTAG	GTCTCCGTTTGTGGTGACAT	20	20	55.0	50.0	57.1	58.1	0.0	9.4	0.974778	56371	57310	964-993	1.000000	76.2
atpB-rbcL_p5	atpB-rbcL	5	GGGCCAATAATTTGAGCGATG	GTCTCCGTTTGTGGTGACAT	21	20	47.6	50.0	58.0	58.1	4.4	9.4	1.138312	56371	57310	1017-1046	1.000000	74.6
petA-psbJ_p1	petA-psbJ	1	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	58.7	58.0	17.6	0.0	0.722382	65018	66151	1265-1270	1.000000	76.1
petA-psbJ_p2	petA-psbJ	2	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	58.7	58.1	17.6	0.0	0.801849	65018	66151	1266-1271	1.000000	75.4
petA-psbJ_p3	petA-psbJ	3	GATCCATTACGCGTCCAAGG	CCGGATTGGGTTCATCTCTG	20	20	55.0	55.0	58.8	58.0	18.6	0.0	0.823077	65018	66151	1276-1281	1.000000	75.2
petA-psbJ_p4	petA-psbJ	4	GATCCATTACGCGTCCAAGG	TCCGGATTGGGTTCATCTCT	20	20	55.0	50.0	58.8	58.1	18.6	0.0	0.902543	65018	66151	1277-1282	1.000000	74.6
petA-psbJ_p5	petA-psbJ	5	TCCAAGGCCTTTTGTTCTTCT	CCGGATTGGGTTCATCTCTG	21	20	42.9	55.0	57.7	58.0	17.6	0.0	1.353694	65018	66151	1263-1268	1.000000	71.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.5	6.4	0.0	0.541273	66931	67733	800-952	1.000000	80.0
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.5	0.0	0.0	0.578888	66931	67733	813-965	1.000000	79.7
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	20	20	45.0	45.0	58.2	57.5	0.0	0.0	0.660959	66931	67733	809-961	1.000000	79.1
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.677289	66931	67733	808-960	1.000000	78.9
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.727231	66931	67733	802-954	1.000000	78.5
petG-trnW-CCA_p1	petG-trnW-CCA	1	GCGATCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.2	58.0	0.0	2.6	0.207617	68112	68225	209-212	1.000000	81.0
petG-trnW-CCA_p2	petG-trnW-CCA	2	GCGATCAGTTGGACCTTTGA	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.2	57.9	0.0	1.4	0.280539	68112	68225	173-176	1.000000	81.0
petG-trnW-CCA_p3	petG-trnW-CCA	3	GCGATCAGTTGGACCTTTGA	CCCGATGTCGTAGGTTCAAA	20	20	50.0	50.0	58.2	57.6	0.0	1.4	0.565130	68112	68225	168-171	1.000000	78.8
petG-trnW-CCA_p4	petG-trnW-CCA	4	GCGATCAGTTGGACCTTTGA	TCCAAAACCCGATGTCGTAG	20	20	50.0	50.0	58.2	57.6	0.0	1.4	0.565130	68112	68225	175-178	1.000000	78.7
petG-trnW-CCA_p5	petG-trnW-CCA	5	GCGATCAGTTGGACCTTTGA	CGCTCTTAGTTCAGTTCGGT	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.540886	68112	68225	207-210	1.000000	78.4
psbB-psbT_p1	psbB-psbT	1	GGCGCTAGAACCTTATTCAGA	TTTTAGTCGGAACCTTGGGC	21	20	47.6	50.0	57.5	58.1	7.6	0.0	1.554126	75769	75958	404-407	1.000000	68.9
psbB-psbT_p2	psbB-psbT	2	GGCGCTAGAACCTTATTCAG	TTTTAGTCGGAACCTTGGGC	20	20	50.0	50.0	56.0	58.1	7.6	0.0	2.075820	75769	75958	404-407	1.000000	64.8
psbB-psbT_p3	psbB-psbT	3	TGCTCAAGTAGAATTTGGAGCA	TTTTAGTCGGAACCTTGGGC	22	20	40.9	50.0	58.0	58.1	9.8	0.0	2.119546	75769	75958	351-354	1.000000	64.6
psbB-psbT_p4	psbB-psbT	4	TGGAGATCCAACTACAAGGAGA	TTTTAGTCGGAACCTTGGGC	22	20	45.5	50.0	57.9	58.1	0.0	0.0	2.160885	75769	75958	318-321	1.000000	64.5
psbB-psbT_p5	psbB-psbT	5	GCGCTAGAACCTTATTCAGAGA	TTTTAGTCGGAACCTTGGGC	22	20	45.5	50.0	57.7	58.1	0.0	0.0	2.408654	75769	75958	403-406	1.000000	62.1
petD-rpoA_p1	petD-rpoA	1	CTCGAGCCGGATGATGAAAA	CTTTTCTACACGCGGAGGAA	20	20	50.0	50.0	58.1	57.9	5.0	12.5	0.134573	79610	79762	1081-1087	1.000000	80.7
petD-rpoA_p2	petD-rpoA	2	CTCGAGCCGGATGATGAAAA	TTTTCTACACGCGGAGGAAG	20	20	50.0	50.0	58.1	57.9	5.0	12.5	0.134573	79610	79762	1080-1086	1.000000	80.7
petD-rpoA_p3	petD-rpoA	3	CTCGAGCCGGATGATGAAAA	ATCCATTTGCGTTAGGGGTC	20	20	50.0	50.0	58.1	57.9	5.0	0.0	0.112808	79610	79762	1446-1452	1.000000	80.6
petD-rpoA_p4	petD-rpoA	4	CTCGAGCCGGATGATGAAAA	TTTGCGTTAGGGGTCCTAGA	20	20	50.0	50.0	58.1	58.1	5.0	0.0	0.118648	79610	79762	1441-1447	1.000000	80.5
petD-rpoA_p5	petD-rpoA	5	CTCGAGCCGGATGATGAAAA	TAGCTAACCTGACGGAACCA	20	20	50.0	50.0	58.1	58.1	5.0	0.0	0.132538	79610	79762	1357-1363	1.000000	80.5
ndhF_p1	ndhF	1	TTTGAACGGACCTCATCGAG	TCCAATCAATCAGTTCCGCA	20	20	50.0	45.0	57.6	57.5	0.0	0.0	0.851992	112977	115178	2699-2849	1.000000	78.3
ndhF_p2	ndhF	2	TTTTGAACGGACCTCATCGA	TCCAATCAATCAGTTCCGCA	20	20	45.0	45.0	57.2	57.5	0.0	0.0	1.312129	112977	115178	2700-2850	1.000000	74.6
ndhF_p3	ndhF	3	TTTGAACGGACCTCATCGAG	ATCCAATCAATCAGTTCCGCA	20	21	50.0	42.9	57.6	58.0	0.0	0.0	1.367182	112977	115178	2700-2850	1.000000	74.2
ndhF_p4	ndhF	4	TTTGAACGGACCTCATCGAG	TCAATCAGTTCCGCAATGAGT	20	21	50.0	42.9	57.6	57.9	0.0	0.0	1.421757	112977	115178	2694-2844	1.000000	73.7
ndhF_p5	ndhF	5	TTTGAACGGACCTCATCGAG	TCCAATCAATCAGTTCCGCAA	20	21	50.0	42.9	57.6	58.2	0.0	0.0	1.574574	112977	115178	2699-2849	1.000000	72.5
ycf1_p1	ycf1	1	TCTGGGAAAAACCAAGGTTCT	TGATTGGAGAATTCTTCATTTCTGT	21	25	42.9	32.0	57.3	57.0	4.7	17.3	7.688638	128057	128176	231-261	1.000000	43.2
ycf1_p2	ycf1	2	TCTGGGAAAAACCAAGGTTCT	TGGAGAATTCTTCATTTCTGT	21	21	42.9	33.3	57.3	52.7	4.7	12.7	7.947563	128057	128176	227-257	1.000000	43.2
ycf1_p3	ycf1	3	TCTGGGAAAAACCAAGGTTCT	TTGGAGAATTCTTCATTTCTGT	21	22	42.9	31.8	57.3	53.6	4.7	17.3	8.051236	128057	128176	228-258	1.000000	43.2
ycf1_p4	ycf1	4	TTCTGGGAAAAACCAAGGTTCT	TGATTGGAGAATTCTTCATTTCTGT	22	25	40.9	32.0	58.0	57.0	4.7	17.3	8.018805	128057	128176	232-262	1.000000	43.1
ycf1_p5	ycf1	5	AGCACAAATTCTGGGAAAAACC	TGATTGGAGAATTCTTCATTTCTGT	22	25	40.9	32.0	57.9	57.0	0.0	17.3	8.079822	128057	128176	240-270	1.000000	43.0