primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GCTCAACCCACAGTAACTGT	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.424537	3987	4717	746-847	1.000000	88.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GCTCAACCCACAGTAACTGT	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.424537	3987	4717	745-846	1.000000	88.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	CTTGAAAATGGCGCTCAACC	20	20	50.0	50.0	58.2	58.3	20.3	7.6	0.466716	3987	4717	758-859	1.000000	88.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	CCTTGAAAATGGCGCTCAAC	20	20	50.0	50.0	58.2	58.3	20.3	7.6	0.466716	3987	4717	758-859	1.000000	88.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAATGGCGCTCAACC	20	20	50.0	50.0	58.2	58.3	20.3	7.6	0.466716	3987	4717	757-858	1.000000	88.3
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	12648	13533	895-988	0.969697	83.6
atpH-atpI_p2	atpH-atpI	2	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.2	58.5	1.4	0.0	0.701648	12648	13533	896-989	0.969697	82.5
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.732109	12648	13533	910-1003	0.969697	82.1
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	12648	13533	868-961	0.909091	80.4
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.2	57.4	1.4	21.3	0.770985	12648	13533	869-962	0.909091	79.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AAGCCCCGATTAATGAACCC	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.543608	25823	26493	811-834	1.000000	87.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AAGCCCCGATTAATGAACCC	CCTTACCACTTGGCCATGTC	20	20	50.0	55.0	57.9	58.5	0.0	20.8	0.603691	25823	26493	765-788	1.000000	87.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AAGCCCCGATTAATGAACCC	TTTGTCAATCAGGCGACACC	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.832544	25823	26493	823-846	1.000000	84.9
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCCCGATTAATGAACCCACA	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.904786	25823	26493	808-831	1.000000	84.5
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AAGCCCCGATTAATGAACCC	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.9	57.5	0.0	14.6	0.592470	25823	26493	786-809	0.939394	84.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGCGAGAATATTAACTTCCA	CCTCGCTGGGATTGTAGTTC	20	20	35.0	55.0	52.0	58.1	0.0	0.0	6.055544	27317	28374	1101-1160	1.000000	51.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TGCGAGAATATTAACTTCCA	TACACCTCGCTGGGATTGTA	20	20	35.0	50.0	52.0	58.1	0.0	10.4	6.141574	27317	28374	1105-1164	1.000000	51.7
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATGCGAGAATATTAACTTCCA	CCTCGCTGGGATTGTAGTTC	21	20	33.3	55.0	52.7	58.1	0.0	0.0	6.328155	27317	28374	1102-1161	1.000000	51.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TGCGAGAATATTAACTTCCAT	CCTCGCTGGGATTGTAGTTC	21	20	33.3	55.0	52.7	58.1	0.0	0.0	6.328155	27317	28374	1101-1160	1.000000	51.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TGCGAGAATATTAACTTCCA	CATACACCTCGCTGGGATTG	20	20	35.0	55.0	52.0	58.4	0.0	0.0	6.410447	27317	28374	1107-1166	1.000000	51.7
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGATCCTAGTTCGGGACTGA	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	57.9	57.8	3.8	0.0	0.307776	28449	28752	415-458	1.000000	89.5
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GGATCCTAGTTCGGGACTGA	GGCAATATGTCTACGCTGGT	20	20	55.0	50.0	57.9	57.8	3.8	0.0	0.309328	28449	28752	414-457	1.000000	89.5
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGATCCTAGTTCGGGACTGA	CGGACTGTAAATTCGTTGGC	20	20	55.0	50.0	57.9	57.5	3.8	0.0	0.589520	28449	28752	431-474	1.000000	87.3
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATCGGATCCTAGTTCGGGAC	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	58.4	57.8	10.8	0.0	0.620454	28449	28752	418-461	1.000000	87.0
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CATCGGATCCTAGTTCGGGA	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	58.4	57.8	10.8	0.0	0.621620	28449	28752	419-462	1.000000	87.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	28969	29643	672-768	1.000000	90.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	28969	29643	670-766	1.000000	90.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	28969	29643	726-822	1.000000	90.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	28969	29643	651-747	1.000000	90.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	28969	29643	649-745	1.000000	90.3
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	TCTACCAAGGGCTACAGTCA	20	20	55.0	50.0	58.1	57.4	7.6	0.0	0.680053	29716	30824	974-1192	1.000000	84.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	TCTACCAAGGGCTACAGTCA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.702208	29716	30824	977-1195	1.000000	84.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	TCTACCAAGGGCTACAGTCA	20	20	50.0	50.0	58.3	57.4	0.0	0.0	0.898792	29716	30824	951-1169	1.000000	83.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GGCCCTTTTAACTCAGTGGT	TCTACCAAGGGCTACAGTCA	20	20	50.0	50.0	57.7	57.4	0.0	0.0	0.903963	29716	30824	989-1207	1.000000	83.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TGGTAGAGTAACGCCATGGT	TCTACCAAGGGCTACAGTCA	20	20	50.0	50.0	58.4	57.4	14.7	0.0	1.052062	29716	30824	973-1191	1.000000	81.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	44107	44878	753-896	1.000000	89.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	44107	44878	785-928	1.000000	88.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	44107	44878	747-890	1.000000	87.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	44107	44878	785-928	1.000000	86.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CAGGTTTCCATACCAAGGCT	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.647198	44107	44878	787-930	1.000000	86.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGGTGCTAAAACTCCGGAAA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	57.4	57.8	5.2	0.0	0.839437	48197	48933	849-870	1.000000	82.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTGGTGCTAAAACTCCGGAA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	57.4	57.8	5.2	0.0	0.839437	48197	48933	850-871	1.000000	82.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATTGGTGCTAAAACTCCGGA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	57.1	57.8	2.9	0.0	1.068380	48197	48933	851-872	1.000000	80.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTGCTAAAACTCCGGAAA	AGCCCAATGCAACAAAATGC	20	20	45.0	45.0	57.4	58.5	5.2	0.0	1.116214	48197	48933	935-955	1.000000	80.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TTGGTGCTAAAACTCCGGAA	AGCCCAATGCAACAAAATGC	20	20	45.0	45.0	57.4	58.5	5.2	0.0	1.116214	48197	48933	936-956	1.000000	80.4
ycf4-cemA_p1	ycf4-cemA	1	TGGTGTACAATTGCGTGGAA	ACAAGCGAAGGAGATACACG	20	20	45.0	50.0	58.0	57.7	5.1	0.0	0.310878	57995	58728	1281-1751	1.000000	88.3
ycf4-cemA_p2	ycf4-cemA	2	TTTCGTTGGGGATTTCCTGG	ACAAGCGAAGGAGATACACG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.361710	57995	58728	1209-1679	1.000000	88.2
ycf4-cemA_p3	ycf4-cemA	3	GCGTGGAATGTAGGTAGTGG	ACAAGCGAAGGAGATACACG	20	20	55.0	50.0	58.1	57.7	0.0	0.0	0.343650	57995	58728	1269-1739	1.000000	88.1
ycf4-cemA_p4	ycf4-cemA	4	AACGGGGTCTCGAAAAACAA	ACAAGCGAAGGAGATACACG	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.320833	57995	58728	1483-1953	1.000000	87.8
ycf4-cemA_p5	ycf4-cemA	5	GGAAATAAGAGGCCAAGGGG	ACAAGCGAAGGAGATACACG	20	20	55.0	50.0	57.9	57.7	5.0	0.0	0.352834	57995	58728	1090-1560	0.969697	87.2
petA-psbJ_p1	petA-psbJ	1	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	13.8	0.0	0.491221	60624	61427	946-967	1.000000	83.4
petA-psbJ_p2	petA-psbJ	2	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.2	57.6	13.8	0.0	0.607328	60624	61427	942-963	1.000000	82.5
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.2	57.6	13.8	0.0	0.609031	60624	61427	945-966	1.000000	82.5
petA-psbJ_p4	petA-psbJ	4	TTACGTGTCCAAGGCCTTTT	GGTTCATATTCCGGGTTGGG	20	20	45.0	55.0	57.6	58.3	17.6	0.0	0.664143	60624	61427	944-965	1.000000	82.1
petA-psbJ_p5	petA-psbJ	5	TACGTGTCCAAGGCCTTTTG	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.4	58.3	17.6	0.0	0.712414	60624	61427	943-964	1.000000	81.7
psbE-petL_p1	psbE-petL	1	ACTGACGAATAACCAACCCG	ACCAATCAATAGAGCTGAGGT	20	21	50.0	42.9	57.9	56.4	0.0	0.0	2.671465	62202	63417	1349-1406	1.000000	67.9
psbE-petL_p2	psbE-petL	2	ACGAATAACCAACCCGCAAT	ACCAATCAATAGAGCTGAGGT	20	21	45.0	42.9	58.2	56.4	0.0	0.0	2.753536	62202	63417	1345-1402	1.000000	67.2
psbE-petL_p3	psbE-petL	3	CCCGCAATAAATAGGGAGGG	ACCAATCAATAGAGCTGAGGT	20	21	55.0	42.9	57.8	56.4	0.0	0.0	2.782888	62202	63417	1333-1390	1.000000	67.0
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	AGCTGAGGTTATAGTTGAAGCA	20	22	50.0	40.9	57.9	57.2	0.0	0.0	2.915224	62202	63417	1337-1394	1.000000	66.0
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGAGGTTATAGTTGAAGCAGCT	20	22	50.0	40.9	57.9	57.2	0.0	0.0	2.915224	62202	63417	1334-1391	1.000000	66.0
rpl36-rps8_p1	rpl36-rps8	1	CACTATAATCCGTCCACGCC	TCGGGGGAGAAATTTTGTGT	20	20	55.0	45.0	58.2	57.3	0.0	0.0	0.888574	77345	77803	560-574	1.000000	80.9
rpl36-rps8_p2	rpl36-rps8	2	ACTATAATCCGTCCACGCCT	TCGGGGGAGAAATTTTGTGT	20	20	50.0	45.0	58.3	57.3	0.0	0.0	0.967189	77345	77803	559-573	1.000000	80.3
rpl36-rps8_p3	rpl36-rps8	3	CACTATAATCCGTCCACGCC	TCGGGGGAGAAATTTTGTGTT	20	21	55.0	42.9	58.2	58.0	0.0	0.0	1.268314	77345	77803	560-574	1.000000	77.9
rpl36-rps8_p4	rpl36-rps8	4	ACTATAATCCGTCCACGCCT	TCGGGGGAGAAATTTTGTGTT	20	21	50.0	42.9	58.3	58.0	0.0	0.0	1.346929	77345	77803	559-573	1.000000	77.3
rpl36-rps8_p5	rpl36-rps8	5	CACTATAATCCGTCCACGCC	ATCGGGGGAGAAATTTTGTGT	20	21	55.0	42.9	58.2	57.8	0.0	0.0	1.389943	77345	77803	561-575	1.000000	76.9
rpl22-rps19_p1	rpl22-rps19	1	TTTCTGTACTCCAACGCGAG	CAAGACATGCAAAAACCGAT	20	20	50.0	40.0	57.9	55.2	15.9	0.0	2.897421	81536	81643	175-222	0.969697	67.6
rpl22-rps19_p2	rpl22-rps19	2	GCGTCCACGAATCTGATCAA	CAAGACATGCAAAAACCGAT	20	20	50.0	40.0	58.4	55.2	2.6	0.0	3.196668	81536	81643	255-302	0.969697	65.2
rpl22-rps19_p3	rpl22-rps19	3	CGTGCTTTGAAAACCGACAT	CAAGACATGCAAAAACCGAT	20	20	45.0	40.0	57.6	55.2	0.0	0.0	3.236491	81536	81643	227-274	0.969697	64.9
rpl22-rps19_p4	rpl22-rps19	4	GAGCGTCCACGAATCTGATC	CAAGACATGCAAAAACCGAT	20	20	55.0	40.0	58.5	55.2	2.6	0.0	3.354818	81536	81643	257-304	0.969697	63.9
rpl22-rps19_p5	rpl22-rps19	5	TTCGTGCTTTGAAAACCGAC	CAAGACATGCAAAAACCGAT	20	20	45.0	40.0	57.5	55.2	0.0	0.0	3.298992	81536	81643	229-276	0.939394	63.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACTGGGCATTCAAAAAGTTT	ACTGCTTCCTAAGAACAGCG	20	20	35.0	50.0	54.1	57.9	0.0	0.0	3.951731	111076	111818	729-885	1.000000	60.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACTGGGCATTCAAAAAGTTT	CTGCTTCCTAAGAACAGCGT	20	20	35.0	50.0	54.1	57.9	0.0	0.0	3.951731	111076	111818	728-884	1.000000	60.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACTGGGCATTCAAAAAGTTT	GCTTCCTAAGAACAGCGTGT	20	20	35.0	50.0	54.1	58.2	0.0	0.0	4.068652	111076	111818	726-882	1.000000	59.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACTGGGCATTCAAAAAGTTT	TGCTTCCTAAGAACAGCGTG	20	20	35.0	50.0	54.1	58.2	0.0	0.0	4.069909	111076	111818	727-883	1.000000	59.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACTGGGCATTCAAAAAGTTTT	CTGCTTCCTAAGAACAGCGT	21	20	33.3	50.0	55.0	57.9	0.0	0.0	4.085204	111076	111818	728-884	1.000000	59.3
ndhG-ndhI_p1	ndhG-ndhI	1	TATTCCCAGGCCCCCAAATA	TCAAACAGTTATGAATTCGACTCA	20	24	50.0	33.3	58.1	56.7	0.0	0.0	5.415634	116418	116811	275-548	1.000000	51.9
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAGGCCCCCAAATATAA	TCAAACAGTTATGAATTCGACTCA	20	24	50.0	33.3	58.1	56.7	0.0	0.0	5.415634	116418	116811	272-545	1.000000	51.9
ndhG-ndhI_p3	ndhG-ndhI	3	TTCCCAGGCCCCCAAATATA	TCAAACAGTTATGAATTCGACTCA	20	24	50.0	33.3	58.1	56.7	0.0	0.0	5.415634	116418	116811	273-546	1.000000	51.9
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAGGCCCCCAAATATAAG	TCAAACAGTTATGAATTCGACTCA	20	24	55.0	33.3	57.7	56.7	0.0	0.0	5.653251	116418	116811	271-544	1.000000	51.9
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAGGCCCCCAAATATAA	CAAACAGTTATGAATTCGACTCA	20	23	50.0	34.8	58.1	55.3	0.0	0.0	5.779221	116418	116811	271-544	1.000000	51.9
ycf1_p1	ycf1	1	TGGTATGGTCCCTTCATGGT	TTGTCTGAAACCTTGGCGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.065752	124259	124378	436-448	0.787879	80.1
ycf1_p2	ycf1	2	TGGTATGGTCCCTTCATGGT	TTTGTCTGAAACCTTGGCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.065752	124259	124378	437-449	0.787879	80.1
ycf1_p3	ycf1	3	TGGTATGGTCCCTTCATGGT	TCTGAAACCTTGGCGAAGAC	20	20	50.0	50.0	58.0	58.1	0.0	10.4	0.141238	124259	124378	433-445	0.727273	77.1
ycf1_p4	ycf1	4	TGGTATGGTCCCTTCATGGT	AGAAGGGGCAAAAGAACAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021267	124259	124378	775-781	0.484848	64.1
ycf1_p5	ycf1	5	TGGTATGGTCCCTTCATGGT	GAAGGGGCAAAAGAACAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024814	124259	124378	774-780	0.484848	64.0