primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH(GUG)-psbA_p1	trnH(GUG)-psbA	1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	94	530	508-574	1.000000	87.0
trnH(GUG)-psbA_p2	trnH(GUG)-psbA	2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	58.2	58.3	0.0	0.0	0.496882	94	530	509-575	1.000000	86.8
trnH(GUG)-psbA_p3	trnH(GUG)-psbA	3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531379	94	530	509-575	1.000000	86.5
trnH(GUG)-psbA_p4	trnH(GUG)-psbA	4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.557166	94	530	510-576	1.000000	86.3
trnH(GUG)-psbA_p5	trnH(GUG)-psbA	5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.7	58.3	0.0	0.0	0.668369	94	530	501-567	1.000000	85.5
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	CGGATCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.1	58.1	0.0	16.9	0.161467	6430	8073	1504-1748	1.000000	85.6
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	ATCTGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.162155	6430	8073	1501-1745	1.000000	85.6
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6430	8073	1490-1734	1.000000	85.2
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	CCACAACGGATCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.3	58.1	7.9	16.9	0.349248	6430	8073	1510-1754	1.000000	84.1
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	GGATCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	0.0	16.9	0.429912	6430	8073	1503-1747	1.000000	83.4
trnS(GCU)-trnG(GCC)_p1	trnS(GCU)-trnG(GCC)	1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.425660	9375	10078	787-818	1.000000	67.0
trnS(GCU)-trnG(GCC)_p2	trnS(GCU)-trnG(GCC)	2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.427048	9375	10078	786-817	1.000000	67.0
trnS(GCU)-trnG(GCC)_p3	trnS(GCU)-trnG(GCC)	3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	57.9	56.6	9.5	0.0	2.515840	9375	10078	851-882	1.000000	66.3
trnS(GCU)-trnG(GCC)_p4	trnS(GCU)-trnG(GCC)	4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9375	10078	852-883	1.000000	65.5
trnS(GCU)-trnG(GCC)_p5	trnS(GCU)-trnG(GCC)	5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	20	21	50.0	42.9	58.2	56.6	9.5	0.0	2.617743	9375	10078	853-884	1.000000	65.5
atpA-atpF_p1	atpA-atpF	1	ACTAATTTCGTCGGCTCGAA	TGTTGGGAGCGATGAAAGAA	20	20	45.0	45.0	57.4	57.4	9.6	0.0	1.201836	12701	12770	124-134	1.000000	78.8
atpA-atpF_p2	atpA-atpF	2	ACTAATTTCGTCGGCTCGAA	ATATTGGCATGTTGGGAGCG	20	20	45.0	50.0	57.4	58.7	9.6	0.0	1.320665	12701	12770	133-143	1.000000	77.5
atpA-atpF_p3	atpA-atpF	3	ACTAATTTCGTCGGCTCGAA	ATGTTGGGAGCGATGAAAGA	20	20	45.0	45.0	57.4	57.2	9.6	0.0	1.429611	12701	12770	125-135	1.000000	76.9
atpA-atpF_p4	atpA-atpF	4	ACTAATTTCGTCGGCTCGAA	CATGTTGGGAGCGATGAAAG	20	20	45.0	50.0	57.4	57.2	9.6	0.0	1.480525	12701	12770	126-136	1.000000	76.5
atpA-atpF_p5	atpA-atpF	5	AATTTCGTCGGCTCGAATGG	TGTTGGGAGCGATGAAAGAA	20	20	50.0	45.0	59.0	57.4	13.2	0.0	1.552865	12701	12770	121-131	1.000000	76.1
trnM(CAU)-psbD_p1	trnM(CAU)-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	33249	34560	1290-1475	1.000000	84.7
trnM(CAU)-psbD_p2	trnM(CAU)-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	33249	34560	1293-1478	1.000000	84.5
trnM(CAU)-psbD_p3	trnM(CAU)-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33249	34560	1280-1465	1.000000	83.6
trnM(CAU)-psbD_p4	trnM(CAU)-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33249	34560	1283-1468	1.000000	83.5
trnM(CAU)-psbD_p5	trnM(CAU)-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	33249	34560	1267-1452	1.000000	83.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.244113	43514	44231	529-852	1.000000	83.7
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	20	20	50.0	45.0	57.9	57.7	4.1	20.4	0.390477	43514	44231	602-925	1.000000	82.4
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	20	20	50.0	50.0	57.9	58.5	4.1	3.4	0.581718	43514	44231	610-933	1.000000	80.9
psaA-ycf3_p4	psaA-ycf3	4	TGTTCTTGAGAAATGGCCCG	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.595145	43514	44231	554-877	1.000000	80.8
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.613689	43514	44231	482-805	1.000000	80.8
trnM(CAU)-trnL(UAA)_p1	trnM(CAU)-trnL(UAA)	1	TGAAGGAAAAGCCGGCTATC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	33.1	0.0	0.043011	48381	49060	776-792	1.000000	83.4
trnM(CAU)-trnL(UAA)_p2	trnM(CAU)-trnL(UAA)	2	TGAAGGAAAAGCCGGCTATC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	33.1	0.0	0.107820	48381	49060	784-800	1.000000	82.9
trnM(CAU)-trnL(UAA)_p3	trnM(CAU)-trnL(UAA)	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48381	49060	724-740	1.000000	82.9
trnM(CAU)-trnL(UAA)_p4	trnM(CAU)-trnL(UAA)	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48381	49060	732-748	1.000000	82.4
trnM(CAU)-trnL(UAA)_p5	trnM(CAU)-trnL(UAA)	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48381	49060	756-772	1.000000	81.9
trnF(GAA)-ndhJ_p1	trnF(GAA)-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	50033	50700	811-815	1.000000	81.1
trnF(GAA)-ndhJ_p2	trnF(GAA)-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	50033	50700	775-779	1.000000	80.7
trnF(GAA)-ndhJ_p3	trnF(GAA)-ndhJ	3	TCAAATCTGGTTCCTGGCAC	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.177438	50033	50700	760-764	1.000000	80.5
trnF(GAA)-ndhJ_p4	trnF(GAA)-ndhJ	4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	58.0	58.2	10.3	0.0	0.194095	50033	50700	846-850	1.000000	80.2
trnF(GAA)-ndhJ_p5	trnF(GAA)-ndhJ	5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.223436	50033	50700	799-803	1.000000	80.0
atpB-rbcL_p1	atpB-rbcL	1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	56500	57279	898-920	1.000000	80.9
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	56500	57279	869-891	1.000000	80.3
atpB-rbcL_p3	atpB-rbcL	3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	56500	57279	940-962	1.000000	79.6
atpB-rbcL_p4	atpB-rbcL	4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	20	20	45.0	45.0	58.2	58.3	0.0	0.0	0.523554	56500	57279	897-919	1.000000	79.2
atpB-rbcL_p5	atpB-rbcL	5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.541987	56500	57279	916-938	1.000000	79.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	67037	68223	1370-1394	1.000000	77.4
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	0.0	0.0	1.030402	67037	68223	1383-1407	1.000000	77.1
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	67037	68223	1293-1317	1.000000	76.9
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	67037	68223	1379-1403	1.000000	76.4
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	67037	68223	1378-1402	1.000000	76.3
rpl16_p1	rpl16	1	CCCCAAGATTCATTTCTGTTGG	TCAATTGACCATTTGATATAATTGCT	22	26	45.5	26.9	57.5	56.1	0.0	3.4	10.400484	83541	84824	1296-1342	1.000000	45.0
rpl16_p2	rpl16	2	CCCCAAGATTCATTTCTGTTGG	TCAATTGACCATTTGATATAATTGC	22	25	45.5	28.0	57.5	54.8	0.0	3.4	10.684716	83541	84824	1296-1342	1.000000	45.0
rpl16_p3	rpl16	3	CCCCAAGATTCATTTCTGTTGG	CAATTGACCATTTGATATAATTGCT	22	25	45.5	28.0	57.5	54.8	0.0	0.0	10.696015	83541	84824	1295-1341	1.000000	45.0
rpl16_p4	rpl16	4	CCCCAAGATTCATTTCTGTTGG	TTCAATTGACCATTTGATATAATTGCT	22	27	45.5	25.9	57.5	56.7	0.0	7.9	10.793068	83541	84824	1297-1343	1.000000	45.0
rpl16_p5	rpl16	5	CCCCAAGATTCATTTCTGTTGG	ATTGACCATTTGATATAATTGCT	22	23	45.5	26.1	57.5	52.5	0.0	0.0	10.955546	83541	84824	1293-1339	1.000000	45.0
rpl22-rps19_p1	rpl22-rps19	1	AAGAGCGTCCACGAATTTGA	AGGACACAAATTGGGCGAAT	20	20	45.0	45.0	57.8	58.1	2.6	0.0	0.240462	86086	86150	247-247	0.041667	39.7
rpl22-rps19_p2	rpl22-rps19	2	AAGAGCGTCCACGAATTTGA	TGGTAGGACACAAATTGGGC	20	20	45.0	50.0	57.8	58.1	2.6	0.0	0.245946	86086	86150	251-251	0.041667	39.7
rpl22-rps19_p3	rpl22-rps19	3	AAGAGCGTCCACGAATTTGA	GTAGGACACAAATTGGGCGA	20	20	45.0	50.0	57.8	58.2	2.6	0.0	0.347744	86086	86150	249-249	0.041667	38.9
rpl22-rps19_p4	rpl22-rps19	4	AGAGCGTCCACGAATTTGAT	AGGACACAAATTGGGCGAAT	20	20	45.0	45.0	57.6	58.1	2.6	0.0	0.463305	86086	86150	246-246	0.041667	38.0
rpl22-rps19_p5	rpl22-rps19	5	AGAGCGTCCACGAATTTGAT	TGGTAGGACACAAATTGGGC	20	20	45.0	50.0	57.6	58.1	2.6	0.0	0.468789	86086	86150	250-250	0.041667	37.9
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	ACGCGTTTTTGATGCAGAAG	20	20	50.0	45.0	58.0	58.0	17.5	5.6	0.068971	114328	114755	566-623	1.000000	84.9
ndhF-rpl32_p2	ndhF-rpl32	2	GTTGCCGTCGGAAAAAGAAG	CGCGTTTTTGATGCAGAAGT	20	20	50.0	45.0	58.0	58.0	17.5	0.0	0.068971	114328	114755	565-622	1.000000	84.9
ndhF-rpl32_p3	ndhF-rpl32	3	TGCCGTCGGAAAAAGAAGAA	ACGCGTTTTTGATGCAGAAG	20	20	45.0	45.0	58.0	58.0	17.5	5.6	0.080260	114328	114755	564-621	1.000000	84.8
ndhF-rpl32_p4	ndhF-rpl32	4	TGCCGTCGGAAAAAGAAGAA	CGCGTTTTTGATGCAGAAGT	20	20	45.0	45.0	58.0	58.0	17.5	0.0	0.080260	114328	114755	563-620	1.000000	84.8
ndhF-rpl32_p5	ndhF-rpl32	5	TTGCCGTCGGAAAAAGAAGA	CGCGTTTTTGATGCAGAAGT	20	20	45.0	45.0	58.0	58.0	17.5	0.0	0.080260	114328	114755	564-621	1.000000	84.8
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	TGGGACAGCGTTAAAAGCAT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	6.9	0.0	0.219990	114918	115779	983-1001	1.000000	83.5
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	TGGGACAGCGTTAAAAGCAT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	6.9	0.0	0.280717	114918	115779	976-994	1.000000	83.1
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	TGGGACAGCGTTAAAAGCAT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	58.1	57.8	6.9	0.0	0.292008	114918	115779	982-1000	1.000000	82.9
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	TGGGACAGCGTTAAAAGCAT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.1	57.8	6.9	0.0	0.292008	114918	115779	981-999	1.000000	82.9
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	TGGGACAGCGTTAAAAGCAT	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	58.1	58.3	6.9	0.0	0.368860	114918	115779	977-995	1.000000	82.3
trnL(UAG)-ccsA_p1	trnL(UAG)-ccsA	1	TAGACACGCTGCTCTTAGGA	ACAATTGAAACGATCGAAAAAGA	20	23	50.0	30.4	57.9	55.7	0.0	24.3	5.407052	115860	115949	213-213	1.000000	42.3
trnL(UAG)-ccsA_p2	trnL(UAG)-ccsA	2	AAATTGGTAGACACGCTGCT	ACAATTGAAACGATCGAAAAAGA	20	23	45.0	30.4	57.8	55.7	0.0	24.3	5.467778	115860	115949	220-220	1.000000	42.2
trnL(UAG)-ccsA_p3	trnL(UAG)-ccsA	3	GTAGACACGCTGCTCTTAGG	ACAATTGAAACGATCGAAAAAGA	20	23	55.0	30.4	57.8	55.7	0.0	24.3	5.479069	115860	115949	214-214	1.000000	42.2
trnL(UAG)-ccsA_p4	trnL(UAG)-ccsA	4	GAGTTCAAGACAAGCCGCTA	ACAATTGAAACGATCGAAAAAGA	20	23	50.0	30.4	57.9	55.7	0.0	24.3	5.363060	115860	115949	245-245	1.000000	42.0
trnL(UAG)-ccsA_p5	trnL(UAG)-ccsA	5	ACAAGCCGCTATGGTGAAAT	ACAATTGAAACGATCGAAAAAGA	20	23	45.0	30.4	57.9	55.7	0.0	24.3	5.411545	115860	115949	236-236	1.000000	42.0
ycf1_p1	ycf1	1	TGAACATTAGTATCAGTTACAATTCT	ACCATTATCAACTGAAATTGTTG	26	23	26.9	30.4	54.8	53.8	0.0	0.0	16.390293	129120	129239	186-189	1.000000	47.6
ycf1_p2	ycf1	2	TGAACATTAGTATCAGTTACAATTCT	AACCATTATCAACTGAAATTGTTG	26	24	26.9	29.2	54.8	54.6	0.0	0.0	16.626714	129120	129239	187-190	1.000000	47.6
ycf1_p3	ycf1	3	TTGAACATTAGTATCAGTTACAATTCT	ACCATTATCAACTGAAATTGTTG	27	23	25.9	30.4	55.4	53.8	0.0	0.0	16.735133	129120	129239	187-190	1.000000	47.6
ycf1_p4	ycf1	4	TGAACATTAGTATCAGTTACAATTC	ACCATTATCAACTGAAATTGTTG	25	23	28.0	30.4	53.4	53.8	0.0	0.0	16.706595	129120	129239	186-189	0.958333	45.5
ycf1_p5	ycf1	5	TTTGAACATTAGTATCAGTTACAA	ACCATTATCAACTGAAATTGTTG	24	23	25.0	30.4	52.4	53.8	0.0	0.0	16.740273	129120	129239	188-191	0.958333	45.5