primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG_p1	trnQ-UUG	1	CCACCTTTTGAAGAGCCCTT	TGTTGACAAAGACCCATCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110200	5800	5868	1045-1164	1.000000	87.5
trnQ-UUG_p2	trnQ-UUG	2	CCACCTTTTGAAGAGCCCTT	GAATCCTTTCGTCCCAGGTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.026664	5800	5868	795-827	0.600000	65.6
trnQ-UUG_p3	trnQ-UUG	3	CCACCTTTTGAAGAGCCCTT	CTGTTCTGTATTGGTGCCGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094382	5800	5868	736-768	0.600000	65.1
trnQ-UUG_p4	trnQ-UUG	4	CCACCTTTTGAAGAGCCCTT	TGTTCTGTATTGGTGCCGAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094382	5800	5868	735-767	0.600000	65.1
trnQ-UUG_p5	trnQ-UUG	5	TGAACAGTTCCTGTGGGTTG	GAATCCTTTCGTCCCAGGTC	20	20	50.0	55.0	57.9	58.0	10.9	0.0	0.076923	5800	5868	889-921	0.600000	65.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GCCCAGGTATTACAGCCATT	AAAGGGGATTTCGCGTCTTT	20	20	50.0	45.0	57.6	58.1	0.0	5.8	0.459335	26329	27461	1274-1340	1.000000	83.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GCCCAGGTATTACAGCCATT	AAGGGGATTTCGCGTCTTTT	20	20	50.0	45.0	57.6	58.1	0.0	5.8	0.459335	26329	27461	1273-1339	1.000000	83.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GCCCTGATCAACGAACCTAC	AAAAGGGGATTTCGCGTCTT	20	20	55.0	45.0	58.3	58.1	0.0	5.8	0.441211	26329	27461	1322-1388	1.000000	83.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GCCCTGATCAACGAACCTAC	AAAGGGGATTTCGCGTCTTT	20	20	55.0	45.0	58.3	58.1	0.0	5.8	0.441211	26329	27461	1321-1387	1.000000	83.2
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GCCCTGATCAACGAACCTAC	AAGGGGATTTCGCGTCTTTT	20	20	55.0	45.0	58.3	58.1	0.0	5.8	0.441211	26329	27461	1320-1386	1.000000	83.2
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGA	20	20	50.0	50.0	58.5	57.9	7.1	0.0	0.576473	29638	29980	397-412	1.000000	82.8
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGAC	20	20	50.0	50.0	58.5	58.2	7.1	0.0	0.668921	29638	29980	396-411	1.000000	82.0
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	29638	29980	413-428	1.000000	81.4
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	29638	29980	412-427	1.000000	81.4
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	29638	29980	434-449	1.000000	80.1
trnE-UUC-trnG-UCC_p1	trnE-UUC-trnG-UCC	1	TGGAGATTCCAATCGCTGTG	GCCTTCCCAAAATGAAAGGC	20	20	50.0	50.0	58.0	57.9	0.0	15.7	0.127561	30197	31234	1840-2031	0.600000	75.0
trnE-UUC-trnG-UCC_p2	trnE-UUC-trnG-UCC	2	GGAGATTCCAATCGCTGTGT	GCCTTCCCAAAATGAAAGGC	20	20	50.0	50.0	58.0	57.9	3.5	15.7	0.128979	30197	31234	1839-2030	0.600000	75.0
trnE-UUC-trnG-UCC_p3	trnE-UUC-trnG-UCC	3	TGGAGATTCCAATCGCTGTG	GCCTTTCATTTTGGGAAGGC	20	20	50.0	50.0	58.0	57.9	0.0	9.3	0.127561	30197	31234	1645-1645	0.200000	47.0
trnE-UUC-trnG-UCC_p4	trnE-UUC-trnG-UCC	4	GGAGATTCCAATCGCTGTGT	GCCTTTCATTTTGGGAAGGC	20	20	50.0	50.0	58.0	57.9	3.5	9.3	0.128979	30197	31234	1644-1644	0.200000	47.0
trnE-UUC-trnG-UCC_p5	trnE-UUC-trnG-UCC	5	TGGAGATTCCAATCGCTGTG	TTGCCCCCAAAATGAAAGGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.137649	30197	31234	1688-1688	0.200000	46.9
rps18-trnG-UCC_p1	rps18-trnG-UCC	1	GCCTTCCCAAAATGAAAGGC	TTCAATAAAAGTCCCCCGCC	20	20	50.0	50.0	57.9	58.2	15.7	0.0	0.257090	31599	31725	1101-1101	0.200000	45.9
rps18-trnG-UCC_p2	rps18-trnG-UCC	2	TCCTTTCATTTTGGGGGCAA	TTCAATAAAAGTCCCCCGCC	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.267177	31599	31725	1058-1058	0.200000	45.9
rps18-trnG-UCC_p3	rps18-trnG-UCC	3	TCAATCGAGTCACCCGTCTA	TTCAATAAAAGTCCCCCGCC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.276261	31599	31725	908-908	0.200000	45.8
rps18-trnG-UCC_p4	rps18-trnG-UCC	4	ACTATATCCGCAAAAGGCCG	TTCAATAAAAGTCCCCCGCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.278995	31599	31725	1136-1136	0.200000	45.8
rps18-trnG-UCC_p5	rps18-trnG-UCC	5	GCCTTCCCAAAATGAAAGGC	AACGAAAAATGGGAGGGAGG	20	20	50.0	50.0	57.9	57.8	15.7	0.0	0.315083	31599	31725	1065-1065	0.200000	45.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	GTTGCGTCTACCAATTTCGC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.254367	46686	47673	1012-1121	1.000000	85.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	CGTTGCGTCTACCAATTTCG	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.272077	46686	47673	1013-1122	1.000000	85.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TCCAATTAAGTCCGTTGCGT	20	20	55.0	45.0	57.9	57.8	0.0	0.0	0.305591	46686	47673	1025-1134	1.000000	85.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TCCGTTGCGTCTACCAATTT	20	20	55.0	45.0	57.9	57.8	0.0	0.0	0.305591	46686	47673	1015-1124	1.000000	85.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GACCGTCGCATTACAAATGC	GTTGCGTCTACCAATTTCGC	20	20	50.0	50.0	58.2	58.1	13.4	0.0	0.311430	46686	47673	1030-1139	1.000000	85.1
psaI-rpl20_p1	psaI-rpl20	1	GTTTTTCGAGACCCCGCTAT	TCGGCTCATCGTGATAGAGT	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.051243	65455	67015	1372-2256	1.000000	91.6
psaI-rpl20_p2	psaI-rpl20	2	GTTTTTCGAGACCCCGCTAT	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.080273	65455	67015	1334-2218	1.000000	91.4
psaI-rpl20_p3	psaI-rpl20	3	GTTTTTCGAGACCCCGCTAT	TCGCAAAATGCTTGCACAAA	20	20	50.0	40.0	58.0	58.1	0.0	9.5	0.088042	65455	67015	1226-2110	1.000000	91.3
psaI-rpl20_p4	psaI-rpl20	4	CCAAGATAACTGGAAGCCCC	TCGGCTCATCGTGATAGAGT	20	20	55.0	50.0	57.9	58.0	0.0	9.5	0.089889	65455	67015	1450-2334	1.000000	91.3
psaI-rpl20_p5	psaI-rpl20	5	TCGAGACCCCGCTATAAGTT	TCGGCTCATCGTGATAGAGT	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.094266	65455	67015	1367-2251	1.000000	91.2
rps12-clpP_p1	rps12-clpP	1	TCGTAGCATTTCTGACTGGC	CCGCGTATGAAGGACTTTGA	20	20	50.0	50.0	58.0	58.0	0.0	4.9	0.014728	68288	69623	803-1613	1.000000	91.9
rps12-clpP_p2	rps12-clpP	2	CCCTCTTAGTCCGTTCTTGC	CCGCGTATGAAGGACTTTGA	20	20	55.0	50.0	58.0	58.0	0.0	4.9	0.016123	68288	69623	1154-1985	1.000000	91.9
rps12-clpP_p3	rps12-clpP	3	CGTAGCATTTCTGACTGGCT	CCGCGTATGAAGGACTTTGA	20	20	50.0	50.0	58.0	58.0	0.0	4.9	0.020318	68288	69623	802-1612	1.000000	91.8
rps12-clpP_p4	rps12-clpP	4	TCGTAGCATTTCTGACTGGC	GAAAGGGTGGTACGTGGATC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.030093	68288	69623	1034-1842	1.000000	91.8
rps12-clpP_p5	rps12-clpP	5	CCCTCTTAGTCCGTTCTTGC	GAAAGGGTGGTACGTGGATC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.031488	68288	69623	1385-2214	1.000000	91.7
psbB-psbT_p1	psbB-psbT	1	TCCAAAAACTTGGCGATCCA	GGAATGTATAAACCAATGCTTCCA	20	24	45.0	37.5	58.0	57.5	0.0	0.0	4.475701	73093	73354	287-331	1.000000	53.5
psbB-psbT_p2	psbB-psbT	2	GGCGATCCAACCACAAAAAG	GGAATGTATAAACCAATGCTTCCA	20	24	50.0	37.5	57.9	57.5	0.0	0.0	4.528568	73093	73354	276-320	1.000000	53.3
psbB-psbT_p3	psbB-psbT	3	ACCACAAAAAGACAGGCAGT	GGAATGTATAAACCAATGCTTCCA	20	24	45.0	37.5	57.9	57.5	0.0	0.0	4.607870	73093	73354	267-311	1.000000	52.9
psbB-psbT_p4	psbB-psbT	4	CCAACCACAAAAAGACAGGC	GGAATGTATAAACCAATGCTTCCA	20	24	50.0	37.5	57.8	57.5	0.0	0.0	4.687531	73093	73354	270-314	1.000000	52.2
psbB-psbT_p5	psbB-psbT	5	TCCAAAAACTTGGCGATCCA	AGGAATGTATAAACCAATGCTTCC	20	24	45.0	37.5	58.0	57.3	0.0	0.0	4.727054	73093	73354	288-332	1.000000	51.5
rpl14-rpl16_p1	rpl14-rpl16	1	GCCACATTCAAATGGGTCTG	ATGTAGAACCAAAAGAAGATTAG	20	23	50.0	30.4	57.3	52.2	7.6	0.0	9.451360	80432	81119	723-743	0.600000	32.2
rpl14-rpl16_p2	rpl14-rpl16	2	TGTTGTCCGCCACATTCAAA	ATGTAGAACCAAAAGAAGATTAG	20	23	45.0	30.4	58.6	52.2	0.0	0.0	9.397568	80432	81119	731-751	0.600000	32.1
rpl14-rpl16_p3	rpl14-rpl16	3	TTGTCCGCCACATTCAAATG	ATGTAGAACCAAAAGAAGATTAG	20	23	45.0	30.4	57.3	52.2	1.2	0.0	9.512445	80432	81119	729-749	0.600000	32.1
rpl14-rpl16_p4	rpl14-rpl16	4	GTTGTCCGCCACATTCAAAT	ATGTAGAACCAAAAGAAGATTAG	20	23	45.0	30.4	57.3	52.2	0.0	0.0	9.514295	80432	81119	730-750	0.600000	32.1
rpl14-rpl16_p5	rpl14-rpl16	5	CGCCACATTCAAATGGGTCT	ATGTAGAACCAAAAGAAGATTAG	20	23	50.0	30.4	58.8	52.2	7.6	0.0	9.614644	80432	81119	724-744	0.600000	32.1
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	ATTCTTGCAATTGCCCAGGA	20	20	50.0	45.0	58.0	58.1	5.8	26.9	0.066453	87460	88056	661-670	1.000000	85.6
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	GTCTCGCGTGTCTACCATTT	TTCTTGCAATTGCCCAGGAT	20	20	50.0	45.0	58.0	58.1	5.8	26.9	0.066453	87460	88056	660-669	1.000000	85.6
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	CTCGCGTGTCTACCATTTCA	ATTCTTGCAATTGCCCAGGA	20	20	50.0	45.0	58.0	58.1	5.8	26.9	0.067824	87460	88056	659-668	1.000000	85.6
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	CTCGCGTGTCTACCATTTCA	TTCTTGCAATTGCCCAGGAT	20	20	50.0	45.0	58.0	58.1	5.8	26.9	0.067824	87460	88056	658-667	1.000000	85.6
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	GTCTCGCGTGTCTACCATTT	CGATTCTTGCAATTGCCCAG	20	20	50.0	50.0	58.0	58.1	5.8	26.9	0.090558	87460	88056	663-672	1.000000	85.4
ErcaC_r001-trnI-GAU_p1	ErcaC_r001-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	95312	95616	388-395	1.000000	82.0
ErcaC_r001-trnI-GAU_p2	ErcaC_r001-trnI-GAU	2	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.7	57.8	7.8	0.0	0.462074	95312	95616	420-427	1.000000	81.8
ErcaC_r001-trnI-GAU_p3	ErcaC_r001-trnI-GAU	3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	57.7	57.8	7.8	4.7	0.464916	95312	95616	439-446	1.000000	81.5
ErcaC_r001-trnI-GAU_p4	ErcaC_r001-trnI-GAU	4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	58.3	57.8	0.0	0.0	0.473195	95312	95616	442-449	1.000000	81.4
ErcaC_r001-trnI-GAU_p5	ErcaC_r001-trnI-GAU	5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	20	20	50.0	50.0	58.3	57.8	0.0	4.7	0.476037	95312	95616	461-468	1.000000	81.2
trnA-UGC-ErcaC_r002_p1	trnA-UGC-ErcaC_r002	1	GGCTGTCAAGAACGTAGAGG	AGTGATGCTTTCGGCTACTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018067	97555	98414	1352-1912	0.800000	83.9
trnA-UGC-ErcaC_r002_p2	trnA-UGC-ErcaC_r002	2	GGGCTGTCAAGAACGTAGAG	AGTGATGCTTTCGGCTACTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018067	97555	98414	1353-1913	0.800000	83.9
trnA-UGC-ErcaC_r002_p3	trnA-UGC-ErcaC_r002	3	GGCTGTCAAGAACGTAGAGG	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	58.0	58.0	0.0	3.6	0.019236	97555	98414	1105-1666	0.800000	83.8
trnA-UGC-ErcaC_r002_p4	trnA-UGC-ErcaC_r002	4	GGGCTGTCAAGAACGTAGAG	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	58.0	58.0	0.0	3.6	0.019236	97555	98414	1106-1667	0.800000	83.8
trnA-UGC-ErcaC_r002_p5	trnA-UGC-ErcaC_r002	5	GGCTGTCAAGAACGTAGAGG	ATGTTTCAGTTCGCCAGGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.021074	97555	98414	1172-1733	0.800000	83.8
ErcaC_r004-trnR-ACG_p1	ErcaC_r004-trnR-ACG	1	AAAAATAGCTCGACGCCAGG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	58.6	57.8	0.0	10.9	0.846282	101766	102005	283-294	1.000000	82.5
ErcaC_r004-trnR-ACG_p2	ErcaC_r004-trnR-ACG	2	GGAAAAATAGCTCGACGCCA	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	58.6	57.8	0.0	10.9	0.850208	101766	102005	285-296	1.000000	82.4
ErcaC_r004-trnR-ACG_p3	ErcaC_r004-trnR-ACG	3	GAAAAATAGCTCGACGCCAG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.3	57.8	0.0	10.9	0.937036	101766	102005	284-295	1.000000	81.7
ErcaC_r004-trnR-ACG_p4	ErcaC_r004-trnR-ACG	4	CTGCGGAAAAATAGCTCGAC	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.3	57.8	0.0	10.9	0.937036	101766	102005	289-300	1.000000	81.6
ErcaC_r004-trnR-ACG_p5	ErcaC_r004-trnR-ACG	5	TGCGGAAAAATAGCTCGACG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	59.0	57.8	0.0	10.9	1.219445	101766	102005	288-299	1.000000	79.4
rpl32-rps15_p1	rpl32-rps15	1	GGCGCTCAAAGCTTTTTCAT	CTTCTTGCGAGGTCACTGAA	20	20	45.0	50.0	57.9	57.8	9.5	0.0	0.225115	105999	107419	364-1558	1.000000	90.2
rpl32-rps15_p2	rpl32-rps15	2	GGCGCTCAAAGCTTTTTCAT	TTCTTCTTGCGAGGTCACTG	20	20	45.0	50.0	57.9	57.8	9.5	0.0	0.225115	105999	107419	366-1560	1.000000	90.2
rpl32-rps15_p3	rpl32-rps15	3	GGCGCTCAAAGCTTTTTCAT	GTAAGGATGAGCCGGAGAAC	20	20	45.0	55.0	57.9	57.8	9.5	5.0	0.296864	105999	107419	785-1979	1.000000	89.6
rpl32-rps15_p4	rpl32-rps15	4	GGCGCTCAAAGCTTTTTCAT	AGTAAGGATGAGCCGGAGAA	20	20	45.0	50.0	57.9	57.8	9.5	5.0	0.230982	105999	107419	1980-1980	0.200000	46.2
rpl32-rps15_p5	rpl32-rps15	5	GGCGCTCAAAGCTTTTTCAT	AGAGTAAGGATGAGCCGGAG	20	20	45.0	55.0	57.9	58.3	9.5	5.0	0.371756	105999	107419	1982-1982	0.200000	45.0