primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnT-GGU_p1	trnfM-CAU-trnT-GGU	1	CCTCAAGGTTATGAGCCTCG	ACCACTAAGTGAAAAGCCCT	20	20	55.0	45.0	57.8	56.7	0.0	0.0	1.579584	13238	16005	2901-2901	1.000000	67.4
trnfM-CAU-trnT-GGU_p2	trnfM-CAU-trnT-GGU	2	CCTCAAGGTTATGAGCCTCG	TGAACCGATGACTTATGCCT	20	20	55.0	45.0	57.8	56.6	0.0	0.0	1.602911	13238	16005	2871-2871	1.000000	67.2
trnfM-CAU-trnT-GGU_p3	trnfM-CAU-trnT-GGU	3	CCTCGTGAGCTACCAAACTG	ACCACTAAGTGAAAAGCCCT	20	20	55.0	45.0	58.3	56.7	0.0	0.0	1.633575	13238	16005	2886-2886	1.000000	67.0
trnfM-CAU-trnT-GGU_p4	trnfM-CAU-trnT-GGU	4	CCTCAAGGTTATGAGCCTCG	GACTTCTGTCTTACCAAGGCA	20	21	55.0	47.6	57.8	57.6	0.0	0.0	1.633842	13238	16005	2929-2929	1.000000	67.0
trnfM-CAU-trnT-GGU_p5	trnfM-CAU-trnT-GGU	5	CCTCAAGGTTATGAGCCTCG	TGACTTCTGTCTTACCAAGGC	20	21	55.0	47.6	57.8	57.6	0.0	0.0	1.633842	13238	16005	2930-2930	1.000000	67.0
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	20	23	50.0	43.5	57.7	57.9	37.2	0.0	3.378932	17260	18317	1216-1216	1.000000	53.0
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	20	23	50.0	43.5	58.5	57.9	5.4	0.0	3.563435	17260	18317	1182-1182	1.000000	51.6
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	20	23	50.0	43.5	57.7	57.7	37.2	0.0	3.639009	17260	18317	1217-1217	1.000000	51.0
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	20	21	50.0	42.9	57.7	55.5	37.2	0.0	3.794602	17260	18317	1217-1217	1.000000	49.7
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	20	20	50.0	40.0	57.7	54.5	37.2	0.0	3.812375	17260	18317	1177-1177	1.000000	49.6
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.240964	20322	21377	394-1210	1.000000	78.1
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.240964	20322	21377	393-1208	1.000000	78.1
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.242473	20322	21377	395-1211	1.000000	78.1
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	20	20	50.0	45.0	58.1	57.8	14.6	5.9	0.279264	20322	21377	394-1198	1.000000	77.8
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	20	20	50.0	50.0	58.1	58.2	14.6	5.9	0.320939	20322	21377	394-1199	1.000000	77.4
rpoC1_p1	rpoC1	1	AGGGAGGATGTTTGATCGGA	ACTAGCGAAATCGGAATGCC	20	20	50.0	50.0	58.1	58.4	0.0	4.8	0.537372	24649	26700	2160-2160	1.000000	75.8
rpoC1_p2	rpoC1	2	AGGGAGGATGTTTGATCGGA	TCTCAATCTGGATTTGCGCG	20	20	50.0	50.0	58.1	59.0	0.0	0.0	1.111347	24649	26700	2194-2194	1.000000	71.2
rpoC1_p3	rpoC1	3	AGGGAGGATGTTTGATCGGA	TAGCGAAATCGGAATGCCC	20	19	50.0	52.6	58.1	58.0	0.0	4.8	1.121241	24649	26700	2158-2158	1.000000	71.1
rpoC1_p4	rpoC1	4	GGGAGGATGTTTGATCGGAA	ACTAGCGAAATCGGAATGCC	20	20	50.0	50.0	57.3	58.4	0.0	4.8	1.127042	24649	26700	2159-2159	1.000000	71.1
rpoC1_p5	rpoC1	5	AGGGAGGATGTTTGATCGGA	CTAGCGAAATCGGAATGCCC	20	20	50.0	55.0	58.1	59.1	0.0	4.8	1.252019	24649	26700	2159-2159	1.000000	70.1
atpA_p1	atpA	1	ATTCTCGGGGCCATAGAATG	ACGATTCCTCTTCTACTAACACG	20	23	50.0	43.5	57.1	57.8	24.0	0.0	4.099719	36558	38081	1698-1698	1.000000	47.2
atpA_p2	atpA	2	ATTCTCGGGGCCATAGAATG	CGATTCCTCTTCTACTAACACGA	20	23	50.0	43.5	57.1	57.5	24.0	0.0	4.348309	36558	38081	1697-1697	1.000000	45.2
atpA_p3	atpA	3	ATTCTCGGGGCCATAGAATG	TCTTTCTCAACGATTCCTCTTCT	20	23	50.0	39.1	57.1	57.3	24.0	0.0	4.597405	36558	38081	1707-1707	1.000000	43.2
atpA_p4	atpA	4	ATTCTCGGGGCCATAGAATG	TCCTCTTCTACTAACACGAGT	20	21	50.0	42.9	57.1	55.2	24.0	0.0	4.648874	36558	38081	1693-1693	1.000000	42.8
atpA_p5	atpA	5	ATTCTCGGGGCCATAGAATG	CGATTCCTCTTCTACTAACACG	20	22	50.0	45.5	57.1	56.1	24.0	0.0	4.727983	36558	38081	1697-1697	1.000000	42.2
atpA-trnR-UCU_p1	atpA-trnR-UCU	1	AGGAACAGCTTGAACGGTTT	AAGAGAAGCGTCCATTGTCT	20	20	45.0	45.0	57.9	56.9	0.0	0.0	1.191922	38082	38218	257-257	1.000000	70.8
atpA-trnR-UCU_p2	atpA-trnR-UCU	2	AGCTTGAACGGTTTTCCCTT	AAGAGAAGCGTCCATTGTCT	20	20	45.0	45.0	57.9	56.9	0.0	0.0	1.206506	38082	38218	251-251	1.000000	70.7
atpA-trnR-UCU_p3	atpA-trnR-UCU	3	ATTCACCGAGCAAGCAGAAA	AAGAGAAGCGTCCATTGTCT	20	20	45.0	45.0	58.1	56.9	0.0	0.0	1.242920	38082	38218	298-298	1.000000	70.3
atpA-trnR-UCU_p4	atpA-trnR-UCU	4	AGGAACAGCTTGAACGGTTT	AAAGAGAAGCGTCCATTGTCT	20	21	45.0	42.9	57.9	57.6	0.0	0.0	1.461808	38082	38218	258-258	1.000000	68.6
atpA-trnR-UCU_p5	atpA-trnR-UCU	5	AGGAACAGCTTGAACGGTTT	AGAAAGAGAAGCGTCCATTGT	20	21	45.0	42.9	57.9	57.6	0.0	0.0	1.461808	38082	38218	260-260	1.000000	68.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48147	48995	922-950	1.000000	82.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48147	48995	914-942	1.000000	81.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48147	48995	892-920	1.000000	81.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48147	48995	912-940	1.000000	80.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48147	48995	921-949	1.000000	80.0
ycf4_p1	ycf4	1	TTCAATTGACCGCTACTGGA	TCCTCGTTTGTTCCTTCCTT	20	20	45.0	45.0	56.9	56.7	7.9	0.0	2.457268	59770	60327	664-664	1.000000	60.3
ycf4_p2	ycf4	2	TCAATTGACCGCTACTGGAT	TCCTCGTTTGTTCCTTCCTT	20	20	45.0	45.0	56.6	56.7	3.4	0.0	2.687665	59770	60327	663-663	1.000000	58.5
ycf4_p3	ycf4	3	TTCAATTGACCGCTACTGGA	ATCCTCGTTTGTTCCTTCCT	20	20	45.0	45.0	56.9	56.5	7.9	0.0	2.690376	59770	60327	665-665	1.000000	58.5
ycf4_p4	ycf4	4	TTCAATTGACCGCTACTGGA	TCCTCGTTTGTTCCTTCCTTT	20	21	45.0	42.9	56.9	57.4	7.9	0.0	2.712210	59770	60327	664-664	1.000000	58.3
ycf4_p5	ycf4	5	TTTCAATTGACCGCTACTGGA	TCCTCGTTTGTTCCTTCCTT	21	20	42.9	45.0	57.6	56.7	7.9	0.0	2.726600	59770	60327	665-665	1.000000	58.2
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	58.1	57.8	3.3	0.0	0.341805	60328	60694	484-484	1.000000	77.4
ycf4-cemA_p2	ycf4-cemA	2	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.399458	60328	60694	500-500	1.000000	77.0
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	CCCAGGGCAAAAGTACGATA	20	20	45.0	50.0	58.1	57.3	3.3	0.0	0.798305	60328	60694	470-470	1.000000	73.8
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	20	20	45.0	50.0	57.8	57.3	0.0	0.0	0.855958	60328	60694	486-486	1.000000	73.3
ycf4-cemA_p5	ycf4-cemA	5	TTGGCCTATTTCTTGCGTGT	GAGAAAGAGACTCCCCAGGG	20	20	45.0	60.0	58.1	58.8	3.3	0.0	0.896098	60328	60694	483-483	1.000000	73.0
petD_p1	petD	1	ACGAAAGAAAATAAGGCGGA	TTCGCGGTGGAATGAATCAA	20	20	40.0	45.0	55.4	58.2	0.0	6.1	2.807950	74734	75956	1282-1282	1.000000	57.6
petD_p2	petD	2	ACGAAAGAAAATAAGGCGGAT	TTCGCGGTGGAATGAATCAA	21	20	38.1	45.0	56.0	58.2	0.0	6.1	3.233344	74734	75956	1282-1282	1.000000	54.2
petD_p3	petD	3	ACGAAAGAAAATAAGGCGGA	ACTTCGCGGTGGAATGAATC	20	20	40.0	50.0	55.4	58.6	0.0	6.1	3.253118	74734	75956	1284-1284	1.000000	54.0
petD_p4	petD	4	ACGAAAGAAAATAAGGCGGA	GACTTCGCGGTGGAATGAAT	20	20	40.0	50.0	55.4	58.6	0.0	6.1	3.253118	74734	75956	1285-1285	1.000000	54.0
petD_p5	petD	5	ACGAAAGAAAATAAGGCGGA	CTTCGCGGTGGAATGAATCA	20	20	40.0	50.0	55.4	58.6	0.0	6.1	3.254102	74734	75956	1283-1283	1.000000	54.0
petD-rpoA_p1	petD-rpoA	1	ATCGGTACTGCAGTAGCTCT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	57.9	57.4	38.7	26.6	0.664962	75957	76173	358-358	1.000000	74.9
petD-rpoA_p2	petD-rpoA	2	TCGGTACTGCAGTAGCTCTT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	58.2	57.4	38.7	26.6	0.769418	75957	76173	357-357	1.000000	74.1
petD-rpoA_p3	petD-rpoA	3	CCAGTAGCTACGACCGTTTT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	57.6	57.4	18.0	26.6	0.963851	75957	76173	382-382	1.000000	72.5
petD-rpoA_p4	petD-rpoA	4	ATCGGTACTGCAGTAGCTCT	TGGGACACTCTAGAGAAGCAT	20	21	50.0	47.6	57.9	57.9	38.7	26.6	1.167452	75957	76173	358-358	1.000000	70.9
petD-rpoA_p5	petD-rpoA	5	CGGTACTGCAGTAGCTCTTT	TGGGACACTCTAGAGAAGCA	20	20	50.0	50.0	57.3	57.4	38.7	26.6	1.261472	75957	76173	356-356	1.000000	70.1
rps11-rpl36_p1	rps11-rpl36	1	CGCAAACCAATACGCACTTT	CGTGGGCGAATTAGAGTCAT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.269956	77693	77866	287-287	1.000000	77.8
rps11-rpl36_p2	rps11-rpl36	2	CTACGCAAACCAATACGCAC	CGTGGGCGAATTAGAGTCAT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.378748	77693	77866	290-290	1.000000	77.0
rps11-rpl36_p3	rps11-rpl36	3	TCTACGTGCACTCTTCCGTA	CGTGGGCGAATTAGAGTCAT	20	20	50.0	50.0	58.2	57.8	16.2	0.0	0.412833	77693	77866	327-327	1.000000	76.7
rps11-rpl36_p4	rps11-rpl36	4	TACGCAAACCAATACGCACT	CGTGGGCGAATTAGAGTCAT	20	20	45.0	50.0	58.2	57.8	0.0	0.0	0.426785	77693	77866	289-289	1.000000	76.6
rps11-rpl36_p5	rps11-rpl36	5	CGTGCACTCTTCCGTAAACT	CGTGGGCGAATTAGAGTCAT	20	20	50.0	50.0	58.2	57.8	16.2	0.0	0.445499	77693	77866	323-323	1.000000	76.4
trnI-CAU-ycf15_p1	trnI-CAU-ycf15	1	GTTGGGCGCTTTAACCATTC	TTCAGGGCCTATCCGAAAGA	20	20	50.0	50.0	58.0	58.1	7.6	5.3	0.135071	84996	87548	2691-2696	1.000000	79.1
trnI-CAU-ycf15_p2	trnI-CAU-ycf15	2	GTTGGGCGCTTTAACCATTC	CTCCGCGGATATCTTTGCTT	20	20	50.0	50.0	58.0	58.1	7.6	31.2	0.138121	84996	87548	2620-2625	1.000000	79.0
trnI-CAU-ycf15_p3	trnI-CAU-ycf15	3	GTTGGGCGCTTTAACCATTC	GCGGATATCTTTGCTTCGGA	20	20	50.0	50.0	58.0	58.1	7.6	2.0	0.143336	84996	87548	2616-2621	1.000000	79.0
trnI-CAU-ycf15_p4	trnI-CAU-ycf15	4	GTTGGGCGCTTTAACCATTC	TCCGCGGATATCTTTGCTTC	20	20	50.0	50.0	58.0	58.1	7.6	27.3	0.143336	84996	87548	2619-2624	1.000000	79.0
trnI-CAU-ycf15_p5	trnI-CAU-ycf15	5	GAGCCAGTTCCAATAGACCG	TTCAGGGCCTATCCGAAAGA	20	20	55.0	50.0	58.1	58.1	0.0	5.3	0.184546	84996	87548	2765-2770	1.000000	78.7
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.064968	88704	89279	509-636	1.000000	83.5
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.066338	88704	89279	507-634	1.000000	83.5
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.2	57.9	0.0	29.4	0.253803	88704	89279	504-631	1.000000	82.0
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GTCTCGCGTGTCTACCATTT	CCCCATTCTTGCAATTGCTC	20	20	50.0	50.0	58.0	57.7	5.8	29.4	0.312582	88704	89279	518-645	1.000000	81.5
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	CTCGCGTGTCTACCATTTCA	CCCCATTCTTGCAATTGCTC	20	20	50.0	50.0	58.0	57.7	5.8	29.4	0.313952	88704	89279	516-643	1.000000	81.5
ndhA_p1	ndhA	1	TGAACCCAGTTAGCATAGGGA	ATGGGGGAAGTTGATCGTTG	21	20	47.6	50.0	58.2	57.9	2.9	0.0	1.288260	114364	116474	2251-2251	1.000000	69.7
ndhA_p2	ndhA	2	TGAACCCAGTTAGCATAGGGA	GGGGGAAGTTGATCGTTGAA	21	20	47.6	50.0	58.2	57.8	2.9	0.0	1.353691	114364	116474	2249-2249	1.000000	69.2
ndhA_p3	ndhA	3	CCCAGTTAGCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	20	20	50.0	50.0	56.6	57.9	0.0	0.0	1.523804	114364	116474	2247-2247	1.000000	67.8
ndhA_p4	ndhA	4	TGAACCCAGTTAGCATAGGG	ATGGGGGAAGTTGATCGTTG	20	20	50.0	50.0	56.6	57.9	0.0	0.0	1.523804	114364	116474	2251-2251	1.000000	67.8
ndhA_p5	ndhA	5	ACCCAGTTAGCATAGGGAAC	ATGGGGGAAGTTGATCGTTG	20	20	50.0	50.0	56.6	57.9	2.9	0.0	1.526154	114364	116474	2248-2248	1.000000	67.8
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	133541	134116	509-636	1.000000	83.5
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	133541	134116	507-634	1.000000	83.5
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	29.4	0.0	0.253803	133541	134116	504-631	1.000000	82.0
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	CCCCATTCTTGCAATTGCTC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.7	58.0	29.4	5.8	0.312582	133541	134116	518-645	1.000000	81.5
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	CCCCATTCTTGCAATTGCTC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.7	58.0	29.4	5.8	0.313952	133541	134116	516-643	1.000000	81.5
ycf15-trnI-CAU_p1	ycf15-trnI-CAU	1	TTCAGGGCCTATCCGAAAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	5.3	7.6	0.135071	135272	137824	2691-2696	1.000000	79.1
ycf15-trnI-CAU_p2	ycf15-trnI-CAU	2	CTCCGCGGATATCTTTGCTT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	31.2	7.6	0.138121	135272	137824	2620-2625	1.000000	79.0
ycf15-trnI-CAU_p3	ycf15-trnI-CAU	3	GCGGATATCTTTGCTTCGGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	2.0	7.6	0.143336	135272	137824	2616-2621	1.000000	79.0
ycf15-trnI-CAU_p4	ycf15-trnI-CAU	4	TCCGCGGATATCTTTGCTTC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	27.3	7.6	0.143336	135272	137824	2619-2624	1.000000	79.0
ycf15-trnI-CAU_p5	ycf15-trnI-CAU	5	TTCAGGGCCTATCCGAAAGA	GAGCCAGTTCCAATAGACCG	20	20	50.0	55.0	58.1	58.1	5.3	0.0	0.184546	135272	137824	2765-2770	1.000000	78.7