primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ATCCACAGCGAATCATGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5618	6628	809-1111	1.000000	85.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATCCACAGCGAATCATGTCC	GTCACTCGGAGGTTCGAATC	20	20	50.0	55.0	58.0	58.1	0.0	10.2	0.130271	5618	6628	817-1119	1.000000	85.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5618	6628	786-1088	1.000000	84.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GTCACTCGGAGGTTCGAATC	20	20	45.0	55.0	58.1	58.1	8.1	10.2	0.228636	5618	6628	794-1096	1.000000	84.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCCACAGCGAATCATGTCC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.0	57.8	0.0	16.9	0.252311	5618	6628	813-1115	1.000000	84.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.3	56.4	0.0	0.0	1.897349	7918	8802	968-1031	1.000000	72.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.3	56.4	0.0	0.0	1.898577	7918	8802	969-1032	1.000000	72.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGATTAGCAATCTGCCGCTT	AACCGAAAGACCCCTTAACT	20	20	45.0	45.0	57.7	56.4	9.6	0.0	1.974993	7918	8802	976-1039	1.000000	71.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	20	20	55.0	45.0	58.3	56.4	0.0	0.0	1.954878	7918	8802	1022-1085	1.000000	71.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	57.6	56.4	0.9	0.0	2.046364	7918	8802	960-1023	1.000000	71.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TTGGCTGACTGTTTTTACGT	ATGTATGCTATACGCGCCTC	20	20	40.0	50.0	55.8	57.7	0.0	5.7	2.461054	29536	30536	1189-1204	1.000000	63.6
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TGGCTGACTGTTTTTACGTAGA	ATGTATGCTATACGCGCCTC	22	20	40.9	50.0	57.7	57.7	0.0	5.7	2.604610	29536	30536	1188-1203	1.000000	62.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TTGGCTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	20	20	40.0	50.0	55.8	58.5	0.0	5.4	2.658804	29536	30536	1156-1171	1.000000	62.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TTTGGCTGACTGTTTTTACGT	ATGTATGCTATACGCGCCTC	21	20	38.1	50.0	56.6	57.7	0.0	5.7	2.688487	29536	30536	1190-1205	1.000000	61.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGTGCGAGAATATTGACTTCCA	ATGTATGCTATACGCGCCTC	22	20	40.9	50.0	57.5	57.7	0.0	5.7	2.751487	29536	30536	1116-1131	1.000000	61.5
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	TTGTGGGTCATTTGTGGCAT	GGTGGCTGAGTGGTTGATAG	20	20	45.0	55.0	58.3	58.0	0.0	0.0	0.315298	35004	35142	331-334	1.000000	80.6
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	TTGTGGGTCATTTGTGGCAT	GCTGAGTGGTTGATAGCTCC	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.338714	35004	35142	327-330	1.000000	80.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TTGTGGGTCATTTGTGGCAT	GGCTGAGTGGTTGATAGCTC	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.338714	35004	35142	328-331	1.000000	80.5
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	TTGTGGGTCATTTGTGGCAT	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.344134	35004	35142	320-323	1.000000	80.5
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	AGCTGCAGCAGGATTTGAAA	GGTGGCTGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.0	38.2	0.0	0.405502	35004	35142	294-297	1.000000	80.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	45874	46850	584-1161	1.000000	86.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	45874	46850	592-1169	1.000000	85.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	45874	46850	616-1193	1.000000	85.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	45874	46850	624-1201	1.000000	84.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	45874	46850	607-1184	1.000000	84.4
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.0	58.1	0.0	20.1	1.013432	47440	47914	513-590	1.000000	80.8
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	GTTCAAGTCCCTCTATCCCC	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	56.7	58.1	0.0	20.1	1.356437	47440	47914	514-591	1.000000	78.1
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	GGGTTCAAGTCCCTCTATCC	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	56.7	58.1	0.0	20.1	1.356437	47440	47914	516-593	1.000000	78.0
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GGTTCAAGTCCCTCTATCCC	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	56.7	58.1	0.0	20.1	1.356437	47440	47914	515-592	1.000000	78.0
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	TTCAAGTCCCTCTATCCCCA	TACCAACTGAGCTATCCCGA	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.408944	47440	47914	475-552	1.000000	78.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	47988	48701	451-863	1.000000	85.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.448485	47988	48701	482-894	1.000000	83.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	TCGGGATAGCTCAGTTGGTA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.576179	47988	48701	489-901	1.000000	82.3
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.586136	47988	48701	454-866	1.000000	82.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	20	20	50.0	55.0	58.1	58.6	0.0	3.0	0.658293	47988	48701	450-862	1.000000	81.8
ycf4-cemA_p1	ycf4-cemA	1	ACAAAAAGCTGCGGAATTGG	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.311006	60592	61444	969-1010	1.000000	82.3
ycf4-cemA_p2	ycf4-cemA	2	ACAAAAAGCTGCGGAATTGG	ATGCAAGATAGGGGAGGGAA	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.366118	60592	61444	933-974	1.000000	82.0
ycf4-cemA_p3	ycf4-cemA	3	ACAAAAAGCTGCGGAATTGG	AGATGCAAGATAGGGGAGGG	20	20	45.0	55.0	57.8	58.3	0.0	0.0	0.413616	60592	61444	935-976	1.000000	81.6
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCTGCGGAATTGG	GATGCAAGATAGGGGAGGGA	20	20	45.0	55.0	57.8	58.3	0.0	0.0	0.418712	60592	61444	934-975	1.000000	81.6
ycf4-cemA_p5	ycf4-cemA	5	TGCGGAATTGGCCTATTTCT	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.6	57.8	13.8	0.0	0.593012	60592	61444	960-1001	1.000000	80.0
rpl36-rps8_p1	rpl36-rps8	1	TCAGTCGGCACTTTTCACAA	AGGCGGAATGGGAATTGTAA	20	20	45.0	45.0	58.0	57.2	0.0	0.0	0.850009	80317	80778	613-629	1.000000	78.0
rpl36-rps8_p2	rpl36-rps8	2	TCAGTCGGCACTTTTCACAA	TTAGGCGGAATGGGAATTGT	20	20	45.0	45.0	58.0	57.2	0.0	0.0	0.850009	80317	80778	615-631	1.000000	78.0
rpl36-rps8_p3	rpl36-rps8	3	GGATCAGTCGGCACTTTTCA	AGGCGGAATGGGAATTGTAA	20	20	50.0	45.0	58.2	57.2	0.0	0.0	1.010340	80317	80778	616-632	1.000000	76.7
rpl36-rps8_p4	rpl36-rps8	4	GGATCAGTCGGCACTTTTCA	TTAGGCGGAATGGGAATTGT	20	20	50.0	45.0	58.2	57.2	0.0	0.0	1.010340	80317	80778	618-634	1.000000	76.7
rpl36-rps8_p5	rpl36-rps8	5	ATCAGTCGGCACTTTTCACA	AGGCGGAATGGGAATTGTAA	20	20	45.0	45.0	57.7	57.2	0.0	0.0	1.073297	80317	80778	614-630	1.000000	76.2
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	85094	85136	179-179	1.000000	80.1
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	85094	85136	178-178	1.000000	80.1
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	85094	85136	180-180	1.000000	80.1
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	85094	85136	193-193	1.000000	80.0
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	58.1	58.2	0.2	35.0	0.281592	85094	85136	207-207	1.000000	78.9
ycf1-ndhF_p1	ycf1-ndhF	1	ACAGGAACAAGAGGGATCCA	ATGGGGGAAGGGGTCAAATA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.053979	111123	111271	687-907	1.000000	88.1
ycf1-ndhF_p2	ycf1-ndhF	2	ACAGGAACAAGAGGGATCCA	TGGGGGAAGGGGTCAAATAT	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.053979	111123	111271	686-906	1.000000	88.1
ycf1-ndhF_p3	ycf1-ndhF	3	AAGAGGGATCCACCGAAGAA	ATGGGGGAAGGGGTCAAATA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.056565	111123	111271	679-899	1.000000	88.1
ycf1-ndhF_p4	ycf1-ndhF	4	AAGAGGGATCCACCGAAGAA	TGGGGGAAGGGGTCAAATAT	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.056565	111123	111271	678-898	1.000000	88.1
ycf1-ndhF_p5	ycf1-ndhF	5	ACAGGAACAAGAGGGATCCA	TATGGGGGAAGGGGTCAAAT	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.053979	111123	111271	703-908	0.952381	85.8
ndhF_p1	ndhF	1	AGTATCCCACGAAGACCTGT	TGGGTGAATCGGAAACAATT	20	20	50.0	40.0	57.8	55.5	0.0	0.0	2.704085	111272	113497	2561-2775	1.000000	62.9
ndhF_p2	ndhF	2	ACAGGAACAAGAGGGATCCA	AATGGGTGAATCGGAAACAA	20	20	50.0	40.0	58.0	55.5	15.2	0.0	2.515572	111272	113497	2872-2932	0.857143	58.2
ndhF_p3	ndhF	3	ACAGGAACAAGAGGGATCCA	TGGGTGAATCGGAAACAATT	20	20	50.0	40.0	58.0	55.5	15.2	0.0	2.515572	111272	113497	2870-2930	0.857143	58.2
ndhF_p4	ndhF	4	AAGAGGGATCCACCGAAGAA	AATGGGTGAATCGGAAACAA	20	20	50.0	40.0	58.0	55.5	15.2	0.0	2.518158	111272	113497	2864-2924	0.857143	58.2
ndhF_p5	ndhF	5	AAGAGGGATCCACCGAAGAA	TGGGTGAATCGGAAACAATT	20	20	50.0	40.0	58.0	55.5	15.2	0.0	2.518158	111272	113497	2862-2922	0.857143	58.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGAATGGTCCATTTTGAGCA	AAAAAGGGGGCCCATTACAG	20	20	40.0	50.0	55.2	58.0	18.3	24.8	2.838360	114374	114874	757-1066	0.666667	48.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGAATGGTCCATTTTGAGCA	AGCGTGTCTACCGATTTCAC	20	20	40.0	50.0	55.2	58.0	18.3	0.0	2.797400	114374	114874	623-931	0.666667	48.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGAATGGTCCATTTTGAGCA	CAGCGTGTCTACCGATTTCA	20	20	40.0	50.0	55.2	58.0	18.3	0.0	2.798771	114374	114874	624-932	0.666667	48.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGAATGGTCCATTTTGAGCA	CATCCCCTTTTCCGACGAAT	20	20	40.0	50.0	55.2	58.0	18.3	0.0	2.827957	114374	114874	893-1202	0.666667	48.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGAATGGTCCATTTTGAGCA	TGGCGAAACCTTGGGTAAAA	20	20	40.0	45.0	55.2	57.9	18.3	0.0	2.858404	114374	114874	1098-1407	0.666667	47.4
ndhE-ndhG_p1	ndhE-ndhG	1	TGCTCTAACCATGTTTCGACT	AGGTGCAATTACTATGGCTCG	21	21	42.9	47.6	57.3	58.2	0.0	0.0	2.839321	118793	118980	305-314	1.000000	62.0
ndhE-ndhG_p2	ndhE-ndhG	2	AGTGCTCTAACCATGTTTCGA	AGGTGCAATTACTATGGCTCG	21	21	42.9	47.6	57.3	58.2	0.0	0.0	2.839321	118793	118980	307-316	1.000000	61.9
ndhE-ndhG_p3	ndhE-ndhG	3	GTGCTCTAACCATGTTTCGAC	AGGTGCAATTACTATGGCTCG	21	21	47.6	47.6	57.3	58.2	0.0	0.0	2.885559	118793	118980	306-315	1.000000	61.6
ndhE-ndhG_p4	ndhE-ndhG	4	AGTGCTCTAACCATGTTTCGA	GGTGCAATTACTATGGCTCG	21	20	42.9	50.0	57.3	56.7	0.0	0.0	3.006925	118793	118980	306-315	1.000000	60.6
ndhE-ndhG_p5	ndhE-ndhG	5	TGCTCTAACCATGTTTCGACT	GGTGCAATTACTATGGCTCG	21	20	42.9	50.0	57.3	56.7	0.0	0.0	3.006925	118793	118980	304-313	1.000000	60.6
rps15_p1	rps15	1	TTTCCAATCGTTCTGGTGCA	ACAACGGCAGTCATTTCAGT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.058416	123861	124130	1071-1086	1.000000	84.4
rps15_p2	rps15	2	TTTCCAATCGTTCTGGTGCA	CCAACAACGGCAGTCATTTC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.076212	123861	124130	1074-1089	1.000000	84.2
rps15_p3	rps15	3	TTTCCAATCGTTCTGGTGCA	CCGAAAGATATATGCCCGCA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.119041	123861	124130	959-973	1.000000	84.2
rps15_p4	rps15	4	CCACCGTGTTACATAAGGCA	ACAACGGCAGTCATTTCAGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.134751	123861	124130	1010-1025	1.000000	84.0
rps15_p5	rps15	5	TTTCTTGTAGCCGGTACAGC	ACAACGGCAGTCATTTCAGT	20	20	50.0	45.0	57.9	58.0	10.2	0.0	0.121631	123861	124130	823-838	0.619048	69.3
rps15-ycf1_p1	rps15-ycf1	1	CGTATGACCATCGAGGAACC	ACATTTGACTTTGGCGAGGA	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.406016	128204	128323	229-229	1.000000	80.9
rps15-ycf1_p2	rps15-ycf1	2	CGTATGACCATCGAGGAACC	TTGACTTTGGCGAGGAATCA	20	20	55.0	45.0	57.9	57.4	0.0	0.0	0.695362	128204	128323	225-225	1.000000	78.7
rps15-ycf1_p3	rps15-ycf1	3	CGTATGACCATCGAGGAACC	TGACTTTGGCGAGGAATCAT	20	20	55.0	45.0	57.9	57.2	0.0	0.0	0.923137	128204	128323	224-224	1.000000	76.9
rps15-ycf1_p4	rps15-ycf1	4	CGTATGACCATCGAGGAACC	TGACTTTGGCGAGGAATCATT	20	21	55.0	42.9	57.9	57.9	0.0	0.0	1.207538	128204	128323	224-224	1.000000	74.6
rps15-ycf1_p5	rps15-ycf1	5	CGTATGACCATCGAGGAACC	TTGACTTTGGCGAGGAATCAT	20	21	55.0	42.9	57.9	57.9	0.0	0.0	1.207538	128204	128323	225-225	1.000000	74.6
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	154883	154925	179-179	1.000000	80.1
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	154883	154925	178-178	1.000000	80.1
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	154883	154925	180-180	1.000000	80.1
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	154883	154925	193-193	1.000000	80.0
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	CACAACCCCTTGGGGTTATC	CGGATGTAGCCAAGTGGATC	20	20	55.0	55.0	58.2	58.1	35.0	0.2	0.281592	154883	154925	207-207	1.000000	78.9