primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	GCAAAACTCAGATTGCTCTT	GAGCGTCCGGTGTAATTTCA	20	20	40.0	50.0	54.5	58.3	11.2	0.0	3.791952	1415	3992	2659-2661	1.000000	50.2
trnK-UUU_p2	trnK-UUU	2	GCAAAACTCAGATTGCTCTT	AGCGTCCGGTGTAATTTCAT	20	20	40.0	45.0	54.5	57.6	11.2	0.0	3.905735	1415	3992	2658-2660	1.000000	49.3
trnK-UUU_p3	trnK-UUU	3	GCAAAACTCAGATTGCTCTTT	GAGCGTCCGGTGTAATTTCA	21	20	38.1	50.0	55.3	58.3	11.2	0.0	3.961568	1415	3992	2659-2661	1.000000	48.8
trnK-UUU_p4	trnK-UUU	4	GCAAAACTCAGATTGCTCTT	ATGTTTTGACAGGAGCGTCC	20	20	40.0	50.0	54.5	58.5	11.2	0.0	3.985388	1415	3992	2671-2673	1.000000	48.6
trnK-UUU_p5	trnK-UUU	5	GCAAAACTCAGATTGCTCTTT	AGCGTCCGGTGTAATTTCAT	21	20	38.1	45.0	55.3	57.6	11.2	0.0	4.075351	1415	3992	2658-2660	1.000000	47.9
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAACA	20	21	50.0	47.6	58.3	58.2	5.3	0.0	1.436613	3993	4565	689-710	1.000000	71.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CTTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAAGAACA	20	21	50.0	47.6	57.5	58.2	0.0	0.0	1.698287	3993	4565	694-715	1.000000	69.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAAC	20	20	50.0	50.0	58.3	56.4	5.3	0.0	1.920462	3993	4565	689-710	1.000000	67.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGCACTTAAAAGCCGAGTAC	GGTGCTCAACCTACAAGAACA	20	21	50.0	47.6	57.2	58.2	0.0	0.0	1.936005	3993	4565	688-709	1.000000	67.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCTACCAAGAGTCTGGACGA	GGTGCTCAACCTACAAGAACA	20	21	50.0	47.6	57.1	58.2	13.6	0.0	2.026901	3993	4565	745-766	1.000000	66.5
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.226377	11593	11969	497-497	1.000000	79.0
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.258277	11593	11969	504-504	1.000000	78.7
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.258277	11593	11969	505-505	1.000000	78.7
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.1	57.8	9.5	0.0	0.263697	11593	11969	512-512	1.000000	78.7
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.277359	11593	11969	499-499	1.000000	78.6
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GACAGAAGTCATCGGTTCGA	TGTCCTGAACCACTAGACGA	20	20	50.0	50.0	57.4	57.7	0.0	0.0	0.912957	15882	16351	526-528	1.000000	78.6
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GACAGAAGTCATCGGTTCGA	GCTGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	57.4	58.3	0.0	0.0	0.894807	15882	16351	547-549	1.000000	78.5
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	GACAGAAGTCATCGGTTCGA	CCTGAACCACTAGACGATGG	20	20	50.0	55.0	57.4	57.4	0.0	0.0	1.217676	15882	16351	523-525	1.000000	76.2
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	GACAGAAGTCATCGGTTCGA	CTGAACCACTAGACGATGGG	20	20	50.0	55.0	57.4	57.4	0.0	0.0	1.217676	15882	16351	522-524	1.000000	76.2
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	GACAGAAGTCATCGGTTCGAA	GCTGCCTCCTTGAAAGAGAG	21	20	47.6	55.0	58.1	58.3	0.0	0.0	1.320679	15882	16351	547-549	1.000000	75.1
trnE-UUC-trnY-GUA_p1	trnE-UUC-trnY-GUA	1	ACGGTGCTATCGTCTAGTGA	CGAATTTACAGTCCGTCCCC	20	20	50.0	55.0	58.0	58.4	0.0	0.0	0.385600	16425	16485	198-198	1.000000	77.3
trnE-UUC-trnY-GUA_p2	trnE-UUC-trnY-GUA	2	ACGGTGCTATCGTCTAGTGA	ACGAATTTACAGTCCGTCCC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.409277	16425	16485	199-199	1.000000	77.1
trnE-UUC-trnY-GUA_p3	trnE-UUC-trnY-GUA	3	CTCTCTTTCAAGGAGGCAGC	CGAATTTACAGTCCGTCCCC	20	20	55.0	55.0	58.3	58.4	0.0	0.0	0.614884	16425	16485	149-149	1.000000	75.6
trnE-UUC-trnY-GUA_p4	trnE-UUC-trnY-GUA	4	ACGGTGCTATCGTCTAGTGA	CAAATTTTTGGGCCGAGCTG	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.602609	16425	16485	247-247	1.000000	75.5
trnE-UUC-trnY-GUA_p5	trnE-UUC-trnY-GUA	5	TCGTCTAGTGGTTCAGGACA	CGAATTTACAGTCCGTCCCC	20	20	50.0	55.0	57.7	58.4	0.0	0.0	0.633035	16425	16485	170-170	1.000000	75.4
petN-rpoB_p1	petN-rpoB	1	AGCCCAAGCGAGACTTACTA	CTTCTCAAGTTCTTCCGCCA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.321181	18847	20601	1876-1893	1.000000	78.7
petN-rpoB_p2	petN-rpoB	2	AGCCCAAGCGAGACTTACTA	TCTCAAGTTCTTCCGCCAAG	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.321181	18847	20601	1874-1891	1.000000	78.7
petN-rpoB_p3	petN-rpoB	3	AGCCCAAGCGAGACTTACTA	ACTTCTCAAGTTCTTCCGCC	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.322689	18847	20601	1877-1894	1.000000	78.7
petN-rpoB_p4	petN-rpoB	4	AGCCCAAGCGAGACTTACTA	TTCCGCCAAGCCTTGATTAA	20	20	50.0	45.0	58.2	57.8	0.0	5.9	0.359480	18847	20601	1864-1881	1.000000	78.4
petN-rpoB_p5	petN-rpoB	5	AGCCCAAGCGAGACTTACTA	CTTCCGCCAAGCCTTGATTA	20	20	50.0	50.0	58.2	58.2	0.0	5.9	0.401155	18847	20601	1865-1882	1.000000	78.1
rpoC2-rps2_p1	rpoC2-rps2	1	GAGACCCCGTTTACCCCTAT	ACCAAAATGAACTCCCGCTT	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.205998	30686	30999	410-410	1.000000	79.3
rpoC2-rps2_p2	rpoC2-rps2	2	AGACCCCGTTTACCCCTATT	ACCAAAATGAACTCCCGCTT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.279985	30686	30999	409-409	1.000000	78.7
rpoC2-rps2_p3	rpoC2-rps2	3	CATCAGAGACCCCGTTTACC	ACCAAAATGAACTCCCGCTT	20	20	55.0	45.0	57.7	58.0	0.0	0.0	0.315748	30686	30999	415-415	1.000000	78.4
rpoC2-rps2_p4	rpoC2-rps2	4	GAGACCCCGTTTACCCCTAT	TTTACGCTTTGCCGAGATGT	20	20	55.0	45.0	58.2	58.2	0.0	0.0	0.398040	30686	30999	467-467	1.000000	77.7
rpoC2-rps2_p5	rpoC2-rps2	5	GAGACCCCGTTTACCCCTAT	GGGGACATTTTGGGATTCCA	20	20	55.0	50.0	58.2	57.8	0.0	4.8	0.447465	30686	30999	445-445	1.000000	77.3
ycf3_p1	ycf3	1	TCTTCAACCAAACCACCCTT	TCTATATAGAATGGGTTGGGA	20	21	45.0	38.1	57.2	52.5	0.0	10.1	7.347617	43387	45397	2077-2093	1.000000	40.7
ycf3_p2	ycf3	2	TCTTCAACCAAACCACCCTT	TCTATATAGAATGGGTTGGGAA	20	22	45.0	36.4	57.2	53.4	0.0	10.1	7.420392	43387	45397	2077-2093	1.000000	40.7
ycf3_p3	ycf3	3	TCTTCAACCAAACCACCCTT	TTCTATATAGAATGGGTTGGGA	20	22	45.0	36.4	57.2	53.4	0.0	19.2	7.420392	43387	45397	2078-2094	1.000000	40.7
ycf3_p4	ycf3	4	TCTTCAACCAAACCACCCTT	TTCTATATAGAATGGGTTGGGAA	20	23	45.0	34.8	57.2	54.3	0.0	19.2	7.575905	43387	45397	2078-2094	1.000000	40.7
ycf3_p5	ycf3	5	ATCTTCAACCAAACCACCCT	TCTATATAGAATGGGTTGGGA	20	21	45.0	38.1	56.9	52.5	0.0	10.1	7.581353	43387	45397	2078-2094	1.000000	40.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49125	49719	705-726	1.000000	79.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.213630	49125	49719	758-779	1.000000	78.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	49125	49719	695-716	1.000000	78.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.287539	49125	49719	748-769	1.000000	78.2
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.335347	49125	49719	756-777	1.000000	77.8
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	20	21	50.0	47.6	57.9	58.1	0.0	0.0	1.150630	61690	62593	1040-1041	1.000000	71.6
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	20	21	50.0	47.6	57.9	57.7	0.0	0.0	1.329021	61690	62593	1038-1039	1.000000	70.2
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	20	20	50.0	50.0	57.9	56.3	0.0	0.0	1.732360	61690	62593	1039-1040	1.000000	67.0
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	20	21	50.0	47.6	58.7	58.1	17.6	0.0	1.780175	61690	62593	1047-1048	1.000000	66.6
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGGG	20	21	50.0	52.4	57.9	58.8	0.0	0.0	1.841762	61690	62593	1039-1040	1.000000	66.1
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	TCCCCACTACTGCCCATAAA	AAACCTGCTAAAGCCCCAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.121576	65416	65795	567-567	1.000000	79.7
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.9	57.9	5.7	0.0	0.209515	65416	65795	490-490	1.000000	79.1
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	58.2	57.9	5.7	0.0	0.266772	65416	65795	491-491	1.000000	78.7
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.8	57.9	3.5	0.0	0.285929	65416	65795	498-498	1.000000	78.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CCCCACTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	20	20	55.0	45.0	57.7	57.9	0.0	0.0	0.444015	65416	65795	566-566	1.000000	77.2
rpl20-rps12_p1	rpl20-rps12	1	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054869	67855	68538	855-856	1.000000	79.9
rpl20-rps12_p2	rpl20-rps12	2	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.174323	67855	68538	870-871	1.000000	79.0
rpl20-rps12_p3	rpl20-rps12	3	ACGCATTTTTGTTCGTCGTC	TGCAAGACAGCCAATACGAA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.190679	67855	68538	822-823	1.000000	78.9
rpl20-rps12_p4	rpl20-rps12	4	TTTTTGTTCGTCGTCTCCGA	CGACATGCCAACGGTTAAAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.248398	67855	68538	850-851	1.000000	78.4
rpl20-rps12_p5	rpl20-rps12	5	ACGCATTTTTGTTCGTCGTC	GAACATGCGCTAGGGTGTAT	20	20	45.0	50.0	58.0	57.8	0.0	9.7	0.253779	67855	68538	757-758	1.000000	78.4
psbT-psbN_p1	psbT-psbN	1	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026579	71734	71805	226-226	1.000000	80.5
psbT-psbN_p2	psbT-psbN	2	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035264	71734	71805	221-221	1.000000	80.4
psbT-psbN_p3	psbT-psbN	3	TCTTCTTCCGAGAACCACCT	CCATTCGAAGAACATGGGGA	20	20	50.0	50.0	58.0	57.6	0.0	3.3	0.429490	71734	71805	137-137	1.000000	77.7
psbT-psbN_p4	psbT-psbN	4	TCTTCTTCCGAGAACCACCT	GTCGCCATCTCCATATCTGG	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.387930	71734	71805	227-227	1.000000	77.6
psbT-psbN_p5	psbT-psbN	5	TTCCGAGAACCACCTAAGGT	GTCGCCATCTCCATATCTGG	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.396615	71734	71805	222-222	1.000000	77.5
rpl16_p1	rpl16	1	TCCAGAAAGAAAAACCAGGGG	AGAAGAAAGTATCCCTCTTTC	21	21	47.6	38.1	58.0	52.3	0.0	9.5	7.771483	78765	80261	1614-1628	1.000000	40.4
rpl16_p2	rpl16	2	TCCAGAAAGAAAAACCAGGGG	AAGAAGAAAGTATCCCTCTTTC	21	22	47.6	36.4	58.0	53.2	0.0	9.5	7.856959	78765	80261	1615-1629	1.000000	40.4
rpl16_p3	rpl16	3	TCCAGAAAGAAAAACCAGGGG	AAAGAAGAAAGTATCCCTCTTTC	21	23	47.6	34.8	58.0	54.0	0.0	9.5	8.022126	78765	80261	1616-1630	1.000000	40.4
rpl16_p4	rpl16	4	CCAGAAAGAAAAACCAGGGGT	AGAAGAAAGTATCCCTCTTTC	21	21	47.6	38.1	58.3	52.3	0.0	9.5	8.051043	78765	80261	1613-1627	1.000000	40.4
rpl16_p5	rpl16	5	CCAGAAAGAAAAACCAGGGGT	AAGAAGAAAGTATCCCTCTTTC	21	22	47.6	36.4	58.3	53.2	0.0	9.5	8.136519	78765	80261	1614-1628	1.000000	40.4
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGGGAGAAGAGGA	TCCAAAGATTTTTACGAATACGCT	20	24	55.0	33.3	58.1	57.3	0.0	0.0	4.786783	106606	107460	968-988	1.000000	42.5
ndhF-rpl32_p2	ndhF-rpl32	2	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	106606	107460	940-960	1.000000	42.3
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	106606	107460	941-961	1.000000	42.3
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	106606	107460	942-962	1.000000	42.3
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	106606	107460	943-963	1.000000	42.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.421775	107641	108174	630-633	1.000000	77.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.476741	107641	108174	633-636	1.000000	77.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.542343	107641	108174	603-606	1.000000	76.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.4	57.8	0.0	0.0	0.603070	107641	108174	596-599	1.000000	76.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	20	20	50.0	50.0	58.4	58.2	0.0	4.7	0.604553	107641	108174	617-620	1.000000	76.1
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	TTGAGAACCCCTTGAACGTC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.253552	108332	109297	1159-1159	1.000000	78.5
ccsA_p2	ccsA	2	AAGCATCTCGGTTCGAATCC	TTGAGAACCCCTTGAACGTC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.308519	108332	109297	1156-1156	1.000000	78.0
ccsA_p3	ccsA	3	CTCAAGCATCTCGGTTCGAA	GAGAACCCCTTGAACGTCTT	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.549246	108332	109297	1157-1157	1.000000	76.1
ccsA_p4	ccsA	4	CTCAAGCATCTCGGTTCGAA	TGAGAACCCCTTGAACGTCT	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.583279	108332	109297	1158-1158	1.000000	75.8
ccsA_p5	ccsA	5	AAGCATCTCGGTTCGAATCC	GAGAACCCCTTGAACGTCTT	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.604212	108332	109297	1154-1154	1.000000	75.7