primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGGAAACAAATTGCGTCCAA	20	20	50.0	40.0	58.0	56.7	0.0	13.8	1.287186	10835	11003	287-296	1.000000	75.5
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	TGGAAACAAATTGCGTCCAA	20	20	55.0	40.0	58.1	56.7	0.0	13.8	1.316022	10835	11003	290-299	1.000000	75.2
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	CCCTAGCCTTCCAAGCTAAC	TGGAAACAAATTGCGTCCAA	20	20	55.0	40.0	57.7	56.7	0.0	13.8	1.591036	10835	11003	291-300	1.000000	73.0
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CTAACGATGCGGGTTCGATT	TGGAAACAAATTGCGTCCAA	20	20	50.0	40.0	58.4	56.7	0.0	13.8	1.689844	10835	11003	276-285	1.000000	72.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	TGACGTCGACTATAACCCCT	TGGAAACAAATTGCGTCCAA	20	20	50.0	40.0	57.6	56.7	14.7	13.8	1.682491	10835	11003	307-316	1.000000	71.9
rpl14-rpl16_p1	rpl14-rpl16	1	CGCTGTTGTCCGCTACATTT	GCGTCCAAAATGCCTATACG	20	20	50.0	50.0	59.2	57.3	0.0	0.0	1.866967	84491	84632	218-230	1.000000	73.3
rpl14-rpl16_p2	rpl14-rpl16	2	CGCTGTTGTCCGCTACATT	GCGTCCAAAATGCCTATACG	19	20	52.6	50.0	58.5	57.3	0.0	0.0	2.207279	84491	84632	218-230	1.000000	70.6
rpl14-rpl16_p3	rpl14-rpl16	3	CGCTGTTGTCCGCTACATTT	ATAGCTGCGTCCAAAATGCC	20	20	50.0	50.0	59.2	59.3	0.0	0.0	2.462951	84491	84632	224-236	1.000000	68.5
rpl14-rpl16_p4	rpl14-rpl16	4	CGCTGTTGTCCGCTACATTT	GCTGCGTCCAAAATGCCTAT	20	20	50.0	50.0	59.2	59.3	0.0	0.0	2.462951	84491	84632	221-233	1.000000	68.5
rpl14-rpl16_p5	rpl14-rpl16	5	CGCTGTTGTCCGCTACATTT	CTATAGCTGCGTCCAAAATGC	20	21	50.0	47.6	59.2	57.7	0.0	0.0	2.462894	84491	84632	226-238	1.000000	68.4
ycf1_p1	ycf1	1	TGCACCAAACCATAGCTGTT	GACATACTGAAACGACTCCCA	20	21	45.0	47.6	58.0	57.4	0.0	0.0	1.593841	118510	118629	445-445	0.500000	47.2
ycf1_p2	ycf1	2	TGCACCAAACCATAGCTGTT	TGACATACTGAAACGACTCCC	20	21	45.0	47.6	58.0	57.4	0.0	0.0	1.593841	118510	118629	446-446	0.500000	47.2
ycf1_p3	ycf1	3	TGATTATTGAACCAGCCCGG	GACATACTGAAACGACTCCCA	20	21	50.0	47.6	57.9	57.4	0.0	0.0	1.639131	118510	118629	496-496	0.500000	46.9
ycf1_p4	ycf1	4	TGATTATTGAACCAGCCCGG	TGACATACTGAAACGACTCCC	20	21	50.0	47.6	57.9	57.4	0.0	0.0	1.639131	118510	118629	497-497	0.500000	46.9
ycf1_p5	ycf1	5	ACACCATCCAGTGCAAAGAG	GACATACTGAAACGACTCCCA	20	21	50.0	47.6	58.1	57.4	0.0	0.0	1.686206	118510	118629	885-885	0.500000	46.5
rps15-ndhH_p1	rps15-ndhH	1	TCGGGAGCTCAAAATTCGTT	CCTCTCCACCAATAATGCCC	20	20	45.0	55.0	57.8	58.0	17.2	0.0	0.192030	119467	119643	836-836	0.500000	58.5
rps15-ndhH_p2	rps15-ndhH	2	TTCGGGAGCTCAAAATTCGT	CCTCTCCACCAATAATGCCC	20	20	45.0	55.0	57.8	58.0	17.2	0.0	0.192030	119467	119643	837-837	0.500000	58.5
rps15-ndhH_p3	rps15-ndhH	3	TCGGGAGCTCAAAATTCGTT	CCCCTTCAACTCGTTCCAAA	20	20	45.0	50.0	57.8	58.0	17.2	0.0	0.203252	119467	119643	815-815	0.500000	58.4
rps15-ndhH_p4	rps15-ndhH	4	TTCGGGAGCTCAAAATTCGT	CCCCTTCAACTCGTTCCAAA	20	20	45.0	50.0	57.8	58.0	17.2	0.0	0.203252	119467	119643	816-816	0.500000	58.4
rps15-ndhH_p5	rps15-ndhH	5	TCGGGAGCTCAAAATTCGTT	ATCCAGCCATAGGGGAGATC	20	20	45.0	55.0	57.8	58.0	17.2	0.0	0.214927	119467	119643	714-714	0.500000	58.3
ndhH_p1	ndhH	1	TCGGGAGCTCAAAATTCGTT	ACCCCTATACTGAGATCGGC	20	20	45.0	55.0	57.8	58.1	17.2	0.0	0.252804	119644	120825	1774-1774	0.500000	58.0
ndhH_p2	ndhH	2	TTCGGGAGCTCAAAATTCGT	ACCCCTATACTGAGATCGGC	20	20	45.0	55.0	57.8	58.1	17.2	0.0	0.252804	119644	120825	1775-1775	0.500000	58.0
ndhH_p3	ndhH	3	TCGGGAGCTCAAAATTCGTT	ATACGTTGTTGTACCCCTGC	20	20	45.0	50.0	57.8	57.9	17.2	0.0	0.282449	119644	120825	1567-1567	0.500000	57.7
ndhH_p4	ndhH	4	TTCGGGAGCTCAAAATTCGT	ATACGTTGTTGTACCCCTGC	20	20	45.0	50.0	57.8	57.9	17.2	0.0	0.282449	119644	120825	1568-1568	0.500000	57.7
ndhH_p5	ndhH	5	TCGGGAGCTCAAAATTCGTT	CAACGAATCGCACGTAGAGA	20	20	45.0	50.0	57.8	58.1	17.2	1.2	0.287734	119644	120825	1948-1948	0.500000	57.7
ndhA_p1	ndhA	1	ACCTTCGCCCACTTTTGAAT	CGCCCCAAAGCAATTTGATT	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.102303	120827	122820	2745-2745	0.500000	59.2
ndhA_p2	ndhA	2	ACCTTCGCCCACTTTTGAAT	CTCACCACCCCATAGTTTGG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.133394	120827	122820	2941-2941	0.500000	58.9
ndhA_p3	ndhA	3	CAGGCTCTGGAAGGAATTCC	CGCCCCAAAGCAATTTGATT	20	20	55.0	45.0	58.0	57.9	17.3	0.0	0.147568	120827	122820	2858-2858	0.500000	58.8
ndhA_p4	ndhA	4	CGATCGGAGTGTTTTTCCCT	CGCCCCAAAGCAATTTGATT	20	20	50.0	45.0	57.9	57.9	7.4	0.0	0.192442	120827	122820	2658-2658	0.500000	58.5
ndhA_p5	ndhA	5	ACCTTCGCCCACTTTTGAAT	GACCGATATACCTTGCAGCC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.207599	120827	122820	2384-2384	0.500000	58.3
ndhG_p1	ndhG	1	AATCAAATTGCTTTGGGGCG	TAAAGAAAAGAACGGGCCCC	20	20	45.0	50.0	57.9	58.1	0.0	18.7	0.183902	123818	124351	1105-1105	0.500000	58.5
ndhG_p2	ndhG	2	AATCAAATTGCTTTGGGGCG	AAGAACGGGCCCCTATTAGT	20	20	45.0	50.0	57.9	58.1	0.0	18.7	0.208506	123818	124351	1098-1098	0.500000	58.3
ndhG_p3	ndhG	3	CCAAACTATGGGGTGGTGAG	TAAAGAAAAGAACGGGCCCC	20	20	55.0	50.0	57.9	58.1	0.0	18.7	0.214993	123818	124351	909-909	0.500000	58.3
ndhG_p4	ndhG	4	AATCAAATTGCTTTGGGGCG	GATCCGCTCATGAAAGAGGG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.221358	123818	124351	1742-1742	0.500000	58.2
ndhG_p5	ndhG	5	AATCAAATTGCTTTGGGGCG	GGATCCGCTCATGAAAGAGG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.221358	123818	124351	1743-1743	0.500000	58.2
ndhG-ndhE_p1	ndhG-ndhE	1	TGGACTGTTGGAGATGGAGT	TCTTGGGGCAGAAGCAATTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044037	124352	124608	1210-1210	0.500000	59.6
ndhG-ndhE_p2	ndhG-ndhE	2	TGGACTGTTGGAGATGGAGT	TTCTTGGGGCAGAAGCAATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044037	124352	124608	1211-1211	0.500000	59.6
ndhG-ndhE_p3	ndhG-ndhE	3	TGGACTGTTGGAGATGGAGT	CTTAGCTTTACACCCGTCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.049975	124352	124608	1189-1189	0.500000	59.6
ndhG-ndhE_p4	ndhG-ndhE	4	TTCCGTCTTTGGACTGTTGG	TCTTGGGGCAGAAGCAATTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.052216	124352	124608	1219-1219	0.500000	59.6
ndhG-ndhE_p5	ndhG-ndhE	5	TTCCGTCTTTGGACTGTTGG	TTCTTGGGGCAGAAGCAATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.052216	124352	124608	1220-1220	0.500000	59.6
ndhE_p1	ndhE	1	TGGACTGTTGGAGATGGAGT	TGCTACGAGTTGTCTCATGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033736	124609	124914	1307-1307	0.500000	59.7
ndhE_p2	ndhE	2	TTCCGTCTTTGGACTGTTGG	TGCTACGAGTTGTCTCATGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041915	124609	124914	1316-1316	0.500000	59.7
ndhE_p3	ndhE	3	TGGACTGTTGGAGATGGAGT	TCTTGGGGCAGAAGCAATTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044037	124609	124914	1210-1210	0.500000	59.6
ndhE_p4	ndhE	4	TGGACTGTTGGAGATGGAGT	TTCTTGGGGCAGAAGCAATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044037	124609	124914	1211-1211	0.500000	59.6
ndhE_p5	ndhE	5	TGGACTGTTGGAGATGGAGT	CTTAGCTTTACACCCGTCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.049975	124609	124914	1189-1189	0.500000	59.6
ndhE-psaC_p1	ndhE-psaC	1	TTGCTATGGCTCGTCAATCC	TGCTACGAGTTGTCTCATGC	20	20	50.0	50.0	58.0	58.0	1.7	0.0	0.050149	124915	125171	1072-1072	0.500000	59.6
ndhE-psaC_p2	ndhE-psaC	2	TTGCTATGGCTCGTCAATCC	TCTTGGGGCAGAAGCAATTT	20	20	50.0	45.0	58.0	58.0	1.7	0.0	0.060451	124915	125171	975-975	0.500000	59.5
ndhE-psaC_p3	ndhE-psaC	3	TTGCTATGGCTCGTCAATCC	TTCTTGGGGCAGAAGCAATT	20	20	50.0	45.0	58.0	58.0	1.7	0.0	0.060451	124915	125171	976-976	0.500000	59.5
ndhE-psaC_p4	ndhE-psaC	4	TTGCTATGGCTCGTCAATCC	CTTAGCTTTACACCCGTCCC	20	20	50.0	55.0	58.0	58.0	1.7	0.0	0.066389	124915	125171	954-954	0.500000	59.5
ndhE-psaC_p5	ndhE-psaC	5	GGGGCCCGTTCTTTTCTTTA	TGCTACGAGTTGTCTCATGC	20	20	50.0	50.0	58.1	58.0	17.8	0.0	0.089930	124915	125171	1006-1006	0.500000	59.3
ndhD_p1	ndhD	1	GCATGAGACAACTCGTAGCA	CTGCAATTGTGGCTTCCATG	20	20	50.0	50.0	58.0	58.0	0.0	7.4	0.008291	125544	127046	2032-2032	0.500000	59.9
ndhD_p2	ndhD	2	GCATGAGACAACTCGTAGCA	TCCTTTGGCGAAATCCAACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.015415	125544	127046	2336-2336	0.500000	59.9
ndhD_p3	ndhD	3	AAATTGCTTCTGCCCCAAGA	CTGCAATTGTGGCTTCCATG	20	20	45.0	50.0	58.0	58.0	0.0	7.4	0.018592	125544	127046	2129-2129	0.500000	59.9
ndhD_p4	ndhD	4	AATTGCTTCTGCCCCAAGAA	CTGCAATTGTGGCTTCCATG	20	20	45.0	50.0	58.0	58.0	0.0	7.4	0.018592	125544	127046	2128-2128	0.500000	59.9
ndhD_p5	ndhD	5	GGGACGGGTGTAAAGCTAAG	CTGCAATTGTGGCTTCCATG	20	20	55.0	50.0	58.0	58.0	0.0	7.4	0.024530	125544	127046	2150-2150	0.500000	59.8
ndhD-ccsA_p1	ndhD-ccsA	1	TCAACGGGGCCATTTTACAA	CTGCAATTGTGGCTTCCATG	20	20	45.0	50.0	58.0	58.0	4.4	7.4	0.007784	127047	127299	887-887	0.500000	59.9
ndhD-ccsA_p2	ndhD-ccsA	2	TCAACGGGGCCATTTTACAA	TCCTTTGGCGAAATCCAACA	20	20	45.0	45.0	58.0	58.0	4.4	0.0	0.014908	127047	127299	1191-1191	0.500000	59.9
ndhD-ccsA_p3	ndhD-ccsA	3	TCAACGGGGCCATTTTACAA	ATGCAGCTCTTTTGTGTGGA	20	20	45.0	45.0	58.0	58.0	4.4	0.0	0.017728	127047	127299	1307-1307	0.500000	59.9
ndhD-ccsA_p4	ndhD-ccsA	4	TCAACGGGGCCATTTTACAA	TGCAGCTCTTTTGTGTGGAT	20	20	45.0	45.0	58.0	58.0	4.4	0.0	0.017728	127047	127299	1306-1306	0.500000	59.9
ndhD-ccsA_p5	ndhD-ccsA	5	TTCTCGATGGCTTCTCTTGC	CTGCAATTGTGGCTTCCATG	20	20	50.0	50.0	58.0	58.0	0.0	7.4	0.020318	127047	127299	729-729	0.500000	59.8
ccsA_p1	ccsA	1	TTCTCGATGGCTTCTCTTGC	AATCGGTAGACACGCTGTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	127300	128271	1802-1802	0.500000	59.8
ccsA_p2	ccsA	2	TTCTCGATGGCTTCTCTTGC	GAAATCGGTAGACACGCTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	127300	128271	1804-1804	0.500000	59.8
ccsA_p3	ccsA	3	TTCTCGATGGCTTCTCTTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	127300	128271	1806-1806	0.500000	59.8
ccsA_p4	ccsA	4	TTCTCGATGGCTTCTCTTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024937	127300	128271	1805-1805	0.500000	59.8
ccsA_p5	ccsA	5	TTCTCGATGGCTTCTCTTGC	CACCATCCCACACGACTAAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.025881	127300	128271	1964-1964	0.500000	59.8
trnL-UAG-ndhF_p1	trnL-UAG-ndhF	1	ACAGCGTGTCTACCGATTTC	AGGAACTGGAAGGGGAATGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.067587	128499	129658	1248-1248	0.500000	59.5
trnL-UAG-ndhF_p2	trnL-UAG-ndhF	2	AGCGTGTCTACCGATTTCAC	AGGAACTGGAAGGGGAATGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.067587	128499	129658	1246-1246	0.500000	59.5
trnL-UAG-ndhF_p3	trnL-UAG-ndhF	3	GAACAGCGTGTCTACCGATT	AGGAACTGGAAGGGGAATGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.067587	128499	129658	1250-1250	0.500000	59.5
trnL-UAG-ndhF_p4	trnL-UAG-ndhF	4	CAGCGTGTCTACCGATTTCA	AGGAACTGGAAGGGGAATGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.068957	128499	129658	1247-1247	0.500000	59.4
trnL-UAG-ndhF_p5	trnL-UAG-ndhF	5	TCCACACAAAAGAGCTGCAT	AGGAACTGGAAGGGGAATGA	20	20	45.0	50.0	58.0	57.9	1.0	0.0	0.074283	128499	129658	1903-1903	0.500000	59.4
ndhF_p1	ndhF	1	CGGGTTGAGTTTTTGGTGGA	CCGAGTGGTACGAGGATTTG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.682811	129659	131890	2984-2984	0.500000	54.5
ndhF_p2	ndhF	2	CGGGTTGAGTTTTTGGTGGA	CGAGTGGTACGAGGATTTGG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.682811	129659	131890	2983-2983	0.500000	54.5
ndhF_p3	ndhF	3	TCGGGTTGAGTTTTTGGTGG	CCGAGTGGTACGAGGATTTG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.682811	129659	131890	2985-2985	0.500000	54.5
ndhF_p4	ndhF	4	TCGGGTTGAGTTTTTGGTGG	CGAGTGGTACGAGGATTTGG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.682811	129659	131890	2984-2984	0.500000	54.5
ndhF_p5	ndhF	5	CGGGTTGAGTTTTTGGTGGA	GAAACCGTGGCACAGATCTA	20	20	50.0	50.0	58.6	57.6	0.0	19.1	0.985465	129659	131890	2839-2839	0.500000	52.1