primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TTTTTACTTTAGTATGGGTTGA	TCGTATATAACTGGATTCTTTT	22	22	27.3	27.3	51.0	50.4	0.0	0.0	18.558072	1447	4346	2978-2978	0.166667	6.7
trnK-UUU_p2	trnK-UUU	2	TTTTTACTTTAGTATGGGTTGA	TCGTATATAACTGGATTCTTTTT	22	23	27.3	26.1	51.0	51.4	0.0	0.0	18.602743	1447	4346	2978-2978	0.166667	6.7
trnK-UUU_p3	trnK-UUU	3	TTTTTACTTTAGTATGGGTTGA	ATCGTATATAACTGGATTCTTT	22	22	27.3	27.3	51.0	50.2	0.0	0.0	18.793380	1447	4346	2979-2979	0.166667	6.7
trnK-UUU_p4	trnK-UUU	4	TTTTTACTTTAGTATGGGTTGA	ATCGTATATAACTGGATTCTTTT	22	23	27.3	26.1	51.0	51.2	0.0	0.0	18.824456	1447	4346	2979-2979	0.166667	6.7
trnK-UUU_p5	trnK-UUU	5	TTTTTACTTTAGTATGGGTTGA	ATCGTATATAACTGGATTCTTTTT	22	24	27.3	25.0	51.0	52.1	0.0	0.0	18.936811	1447	4346	2979-2979	0.166667	6.7
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	20	20	45.0	50.0	58.0	57.9	3.7	0.0	0.114007	3313	4309	1349-1485	1.000000	88.9
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCGCGACGAGAATGGAATTT	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	58.0	58.2	3.7	17.1	0.173002	3313	4309	1332-1468	1.000000	88.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCGCGACGAGAATGGAATTT	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	58.0	58.2	3.7	17.1	0.173002	3313	4309	1331-1467	1.000000	88.5
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCGCGACGAGAATGGAATTT	TCTATCCCAATGGGCCATCT	20	20	45.0	50.0	58.0	57.9	3.7	21.5	0.143117	3313	4309	2078-2159	1.000000	88.3
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCGCGACGAGAATGGAATTT	TCGATCCCGAAGAGAAGGAA	20	20	45.0	50.0	58.0	57.9	3.7	0.0	0.143906	3313	4309	2036-2117	1.000000	88.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5989	6892	815-1033	1.000000	88.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5989	6892	819-1037	1.000000	87.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5989	6892	821-1039	1.000000	85.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	ACTCGGAGGTTCGAATCCTT	20	20	45.0	50.0	58.1	58.4	8.1	16.9	0.581091	5989	6892	820-1038	1.000000	85.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5989	6892	807-1025	1.000000	83.5
psbK-psbI_p1	psbK-psbI	1	CTTTGTTTGGCAAGCTGCTT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.122947	7524	7949	508-589	1.000000	88.9
psbK-psbI_p2	psbK-psbI	2	CTTTGTTTGGCAAGCTGCTT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.122947	7524	7949	509-590	1.000000	88.9
psbK-psbI_p3	psbK-psbI	3	CTTTGTTTGGCAAGCTGCTT	TTACGCCCCGGATCATTAGA	20	20	45.0	50.0	58.1	58.3	15.2	0.7	0.348862	7524	7949	510-591	1.000000	87.0
psbK-psbI_p4	psbK-psbI	4	CTTTGTTTGGCAAGCTGCTT	TTCTTCACGCCCAGGATTAC	20	20	45.0	50.0	58.1	57.6	15.2	0.0	0.452558	7524	7949	526-607	1.000000	86.1
psbK-psbI_p5	psbK-psbI	5	TTTGTTTGGCAAGCTGCTTT	ACGCCCCGGATCATTAGATA	20	20	40.0	50.0	57.6	58.1	13.1	0.7	0.466895	7524	7949	507-588	1.000000	86.1
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8315	9388	1039-1178	1.000000	61.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8315	9388	1038-1177	1.000000	61.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8315	9388	1042-1181	1.000000	60.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8315	9388	1041-1180	1.000000	60.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8315	9388	1040-1179	1.000000	60.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCCCTACACAGGCAAATTGT	AGTATCGACAGACCTAGCCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.092578	27070	28488	2280-2329	0.500000	62.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCCCTACACAGGCAAATTGT	GTATCGACAGACCTAGCCCT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.092578	27070	28488	2279-2328	0.500000	62.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GGGCCCATATCCTTGCTTTT	AGTATCGACAGACCTAGCCC	20	20	50.0	55.0	58.2	58.0	19.9	0.0	0.222520	27070	28488	2088-2137	0.500000	61.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GGGCCCATATCCTTGCTTTT	GTATCGACAGACCTAGCCCT	20	20	50.0	55.0	58.2	58.0	19.9	0.0	0.222520	27070	28488	2087-2136	0.500000	61.7
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CAGAAAATACCGGATCCCCC	GTATCGACAGACCTAGCCCT	20	20	55.0	55.0	57.7	58.0	9.5	0.0	0.287878	27070	28488	2297-2346	0.500000	60.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	32035	33118	1163-1232	1.000000	90.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	32035	33118	1142-1211	1.000000	89.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	32035	33118	1139-1208	1.000000	89.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32035	33118	1141-1210	1.000000	89.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	32035	33118	1160-1229	1.000000	89.1
psaJ-rpl33_p1	psaJ-rpl33	1	AGGGGCGGTAAAATGGAATC	AATGCCCGGCGATTCTTTAT	20	20	50.0	45.0	57.9	58.0	0.0	9.3	0.086109	68162	68687	996-1056	0.500000	63.8
psaJ-rpl33_p2	psaJ-rpl33	2	AGGGGCGGTAAAATGGAATC	ATGCCCGGCGATTCTTTATT	20	20	50.0	45.0	57.9	58.0	0.0	9.3	0.086109	68162	68687	995-1055	0.500000	63.8
psaJ-rpl33_p3	psaJ-rpl33	3	GGGGCGGTAAAATGGAATCT	AATGCCCGGCGATTCTTTAT	20	20	50.0	45.0	57.9	58.0	0.0	9.3	0.086109	68162	68687	995-1055	0.500000	63.8
psaJ-rpl33_p4	psaJ-rpl33	4	GGGGCGGTAAAATGGAATCT	ATGCCCGGCGATTCTTTATT	20	20	50.0	45.0	57.9	58.0	0.0	9.3	0.086109	68162	68687	994-1054	0.500000	63.8
psaJ-rpl33_p5	psaJ-rpl33	5	GGGGCGGTAAAATGGAATCT	GATCGACTCCACTCTCTCCA	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.112927	68162	68687	1558-1624	0.500000	62.5
psbT-psbN_p1	psbT-psbN	1	CGGGCTACTTTGAAATCCGA	CACACGCTCATATTCCGGAA	20	20	50.0	50.0	58.0	58.1	0.0	21.4	0.083953	75622	75684	1138-1151	1.000000	83.3
psbT-psbN_p2	psbT-psbN	2	GGGATGGAATAGCTGTTGGG	CACACGCTCATATTCCGGAA	20	20	55.0	50.0	58.0	58.1	0.0	21.4	0.078529	75622	75684	1440-1453	1.000000	82.9
psbT-psbN_p3	psbT-psbN	3	GGGGATGGAATAGCTGTTGG	CACACGCTCATATTCCGGAA	20	20	55.0	50.0	58.0	58.1	0.0	21.4	0.078529	75622	75684	1441-1454	1.000000	82.9
psbT-psbN_p4	psbT-psbN	4	GGGATGGAATAGCTGTTGGG	CCCTAGTCGCCATCTCCATA	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.088386	75622	75684	907-909	1.000000	82.5
psbT-psbN_p5	psbT-psbN	5	GGGGATGGAATAGCTGTTGG	CCCTAGTCGCCATCTCCATA	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.088386	75622	75684	908-910	1.000000	82.5
petD-rpoA_p1	petD-rpoA	1	AGGTATTGGAGCAACTTTACCT	CACTTTGCAATCAATTTACCT	22	21	40.9	33.3	57.1	53.0	0.0	0.0	8.973238	79253	79433	282-292	1.000000	46.7
petD-rpoA_p2	petD-rpoA	2	TGGTTAGGTATTGGAGCAACT	CACTTTGCAATCAATTTACCT	21	21	42.9	33.3	56.6	53.0	0.0	0.0	8.416289	79253	79433	287-297	1.000000	46.6
petD-rpoA_p3	petD-rpoA	3	TGGTTAGGTATTGGAGCAACTT	CACTTTGCAATCAATTTACCT	22	21	40.9	33.3	57.3	53.0	0.0	0.0	8.695064	79253	79433	287-297	1.000000	46.6
petD-rpoA_p4	petD-rpoA	4	TTGGTTAGGTATTGGAGCAACT	CACTTTGCAATCAATTTACCT	22	21	40.9	33.3	57.3	53.0	0.0	0.0	8.695064	79253	79433	288-298	1.000000	46.6
petD-rpoA_p5	petD-rpoA	5	TCTTTGGTTAGGTATTGGAGCA	CACTTTGCAATCAATTTACCT	22	21	40.9	33.3	57.1	53.0	0.0	0.0	8.963865	79253	79433	291-301	1.000000	46.5
rps8-rpl14_p1	rps8-rpl14	1	CGGCAATAATGTCCCTACCC	AGTTTCATTAGCACCCGAAGT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526474	82109	82327	229-316	1.000000	79.8
rps8-rpl14_p2	rps8-rpl14	2	CGGCAATAATGTCCCTACCC	TCATTAGCACCCGAAGTCTT	20	20	55.0	45.0	58.1	56.6	0.0	0.0	1.551161	82109	82327	225-312	1.000000	79.6
rps8-rpl14_p3	rps8-rpl14	3	CGGCAATAATGTCCCTACCC	TTCATTAGCACCCGAAGTCT	20	20	55.0	45.0	58.1	56.6	0.0	0.0	1.551161	82109	82327	226-313	1.000000	79.6
rps8-rpl14_p4	rps8-rpl14	4	CGGCAATAATGTCCCTACCC	TTCATTAGCACCCGAAGTCTT	20	21	55.0	42.9	58.1	57.3	0.0	0.0	1.803620	82109	82327	226-313	1.000000	77.6
rps8-rpl14_p5	rps8-rpl14	5	CGGCAATAATGTCCCTACCC	TTTCATTAGCACCCGAAGTCT	20	21	55.0	42.9	58.1	57.3	0.0	0.0	1.803620	82109	82327	227-314	1.000000	77.6
rpl14_p1	rpl14	1	GTAGACCGGGTCGACTGATA	AAACTGTAGCCAAAGCAGCT	20	20	55.0	45.0	58.0	58.0	26.9	0.0	0.054076	82328	82696	1008-1098	1.000000	88.1
rpl14_p2	rpl14	2	TAGACCGGGTCGACTGATAC	AAACTGTAGCCAAAGCAGCT	20	20	55.0	45.0	58.0	58.0	20.9	0.0	0.054076	82328	82696	1007-1097	1.000000	88.1
rpl14_p3	rpl14	3	TAGACCGGGTCGACTGATAC	GACAAATAGAAGCAGGGCGA	20	20	55.0	50.0	58.0	58.0	20.9	0.0	0.065134	82328	82696	1205-1295	1.000000	86.8
rpl14_p4	rpl14	4	GTAGACCGGGTCGACTGATA	TGGCGGAATGAACCAAGAAA	20	20	55.0	45.0	58.0	58.0	26.9	0.0	0.052665	82328	82696	1573-1856	1.000000	85.4
rpl14_p5	rpl14	5	TAGACCGGGTCGACTGATAC	TGGCGGAATGAACCAAGAAA	20	20	55.0	45.0	58.0	58.0	20.9	0.0	0.052665	82328	82696	1572-1855	1.000000	85.4
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGTCAGTTAACAGCCAACCG	ACTACTGTTGGAATCATGGT	20	20	50.0	40.0	58.1	53.9	8.6	0.0	4.186992	110586	111511	1005-1011	0.333333	27.8
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	ACGACCAGTCAGTTAACAGC	ACTACTGTTGGAATCATGGT	20	20	50.0	40.0	57.8	53.9	8.6	0.0	4.216342	110586	111511	1011-1017	0.333333	27.5
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	AGGATTCGAACCTACGACCA	ACTACTGTTGGAATCATGGT	20	20	50.0	40.0	58.2	53.9	15.0	0.0	4.222576	110586	111511	1024-1030	0.333333	27.4
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GGATTCGAACCTACGACCAG	ACTACTGTTGGAATCATGGT	20	20	55.0	40.0	57.8	53.9	0.0	0.0	4.271251	110586	111511	1023-1029	0.333333	27.0
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	AGTCAGTTAACAGCCAACCG	ACTACTGTTGGAATCATGGTT	20	21	50.0	38.1	58.1	54.8	8.6	0.0	4.303582	110586	111511	1005-1011	0.333333	26.9
ndhF-rpl32_p1	ndhF-rpl32	1	AACCGTCCTAGTTCTACCGT	AAGTTTCGTTCTCAGTCGGG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.356973	111851	112757	1864-2273	0.500000	67.0
ndhF-rpl32_p2	ndhF-rpl32	2	AACCGTCCTAGTTCTACCGT	TTCGTTCTCAGTCGGGGTAT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.363208	111851	112757	1860-2269	0.500000	67.0
ndhF-rpl32_p3	ndhF-rpl32	3	TTTTATTGCGGGACAGCTCA	AAGTTTCGTTCTCAGTCGGG	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.343061	111851	112757	1945-2354	0.500000	66.9
ndhF-rpl32_p4	ndhF-rpl32	4	TTTTATTGCGGGACAGCTCA	TTCGTTCTCAGTCGGGGTAT	20	20	45.0	50.0	57.8	58.2	0.0	0.0	0.349295	111851	112757	1941-2350	0.500000	66.9
ndhF-rpl32_p5	ndhF-rpl32	5	TTTTCACTTGTTGCAACGGG	AAGTTTCGTTCTCAGTCGGG	20	20	45.0	50.0	57.7	57.8	23.8	0.0	0.436224	111851	112757	2126-2126	0.166667	43.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACCTTTTGAATGGCAGTTCC	TGGCGAAACCTTGGGTAAAA	20	20	45.0	45.0	56.5	57.9	0.0	0.0	1.567049	112932	113669	1394-1499	1.000000	77.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACCTTTTGAATGGCAGTTCC	ATCCGCATAAAAGAGCTGCA	20	20	45.0	45.0	56.5	58.0	0.0	1.0	1.540900	112932	113669	1514-1619	1.000000	76.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACCTTTTGAATGGCAGTTCC	TCCGCATAAAAGAGCTGCAT	20	20	45.0	45.0	56.5	58.0	0.0	1.0	1.540900	112932	113669	1513-1618	1.000000	76.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCACCTACCTTTTGAATGGCA	ATCCGCATAAAAGAGCTGCA	21	20	42.9	45.0	57.5	58.0	0.0	1.0	1.559982	112932	113669	1520-1625	1.000000	76.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCACCTACCTTTTGAATGGCA	TCCGCATAAAAGAGCTGCAT	21	20	42.9	45.0	57.5	58.0	0.0	1.0	1.559982	112932	113669	1519-1624	1.000000	76.7
ndhE_p1	ndhE	1	AGCTCGAACACATTGGGTAC	ATGGTGTTCTTCGGCTGATC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.124600	117566	117748	1280-1321	0.666667	72.8
ndhE_p2	ndhE	2	AGCTCGAACACATTGGGTAC	ACCCATCAATGCATGGTGTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.153385	117566	117748	1292-1333	0.666667	72.5
ndhE_p3	ndhE	3	AGCTCGAACACATTGGGTAC	TGACTGTACCGGCTACAAGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.180984	117566	117748	1346-1387	0.666667	72.5
ndhE_p4	ndhE	4	ACATCCGTCCCAAGGTATCA	ATGGTGTTCTTCGGCTGATC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.155709	117566	117748	1322-1363	0.666667	72.4
ndhE_p5	ndhE	5	ACATCCGTCCCAAGGTATCA	ACCCATCAATGCATGGTGTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.184493	117566	117748	1334-1375	0.666667	72.2
ycf1_p1	ycf1	1	AAAAAGGTCGTTTTCGGGGA	GGGCCTCTTTCAAGCAAATG	20	20	45.0	50.0	57.9	57.6	3.9	0.8	0.430857	121937	122056	647-662	1.000000	85.1
ycf1_p2	ycf1	2	TTTTCGGGGAGAACCAAAGG	GGGCCTCTTTCAAGCAAATG	20	20	50.0	50.0	58.0	57.6	0.0	0.8	0.385467	121937	122056	637-652	0.833333	78.5
ycf1_p3	ycf1	3	TTTTCGGGGAGAACCAAAGG	CGCGCTCAAAGACGTAAAAC	20	20	50.0	50.0	58.0	58.4	0.0	5.6	0.433072	121937	122056	666-681	0.833333	77.9
ycf1_p4	ycf1	4	TTTTCGGGGAGAACCAAAGG	TCCGTAGAACTTCACCGTCA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.402239	121937	122056	1161-1167	0.666667	68.8
ycf1_p5	ycf1	5	TTTTCGGGGAGAACCAAAGG	CCGTAGAACTTCACCGTCAA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.437525	121937	122056	1160-1166	0.666667	68.5