primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	CTTTCGCTTTCCCAGAAGGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.1	57.8	0.0	1.1	0.272272	8556	8703	288-372	1.000000	85.2
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	CTTTCGCTTTCCCAGAAGGT	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.281352	8556	8703	360-444	1.000000	84.9
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	CTTTCGCTTTCCCAGAAGGT	ATGGTTATTTTGTGGGCGGA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.326243	8556	8703	326-410	0.971429	83.5
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	CTTTCGCTTTCCCAGAAGGT	TGGTTATTTTGTGGGCGGAT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.326243	8556	8703	325-409	0.971429	83.5
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	ACCTATCGCTTTCGCTTTCC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	0.0	1.1	0.516681	8556	8703	296-380	1.000000	83.1
ycf3_p1	ycf3	1	CCTGGGGTGGACATAATTGG	CATTCCAAAAGGAACTGAAGCA	20	22	55.0	40.9	57.9	57.4	0.0	0.0	2.660994	14189	15978	1929-1964	1.000000	64.6
ycf3_p2	ycf3	2	CCTGGGGTGGACATAATTGG	TCCAAAAGGAACTGAAGCAA	20	20	55.0	40.0	57.9	55.4	0.0	0.0	2.695196	14189	15978	1926-1961	1.000000	64.4
ycf3_p3	ycf3	3	CCTGGGGTGGACATAATTGG	TTCCAAAAGGAACTGAAGCA	20	20	55.0	40.0	57.9	55.4	0.0	0.0	2.695196	14189	15978	1927-1962	1.000000	64.3
ycf3_p4	ycf3	4	CCTGGGGTGGACATAATTGG	TCCAAAAGGAACTGAAGCAAA	20	21	55.0	38.1	57.9	56.2	0.0	0.0	2.888446	14189	15978	1926-1961	1.000000	62.8
ycf3_p5	ycf3	5	CCTGGGGTGGACATAATTGG	TTCCAAAAGGAACTGAAGCAA	20	21	55.0	38.1	57.9	56.2	0.0	0.0	2.888446	14189	15978	1927-1962	1.000000	62.8
rps4-psbM_p1	rps4-psbM	1	CTGTTGACCCCTGAGCATTT	AAGTGGTAAGGCAGAGGACT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.116354	16877	17204	1044-1075	1.000000	86.1
rps4-psbM_p2	rps4-psbM	2	TGCCGTAATTGTCGTTCTGT	AAGTGGTAAGGCAGAGGACT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.189329	16877	17204	1006-1037	1.000000	85.8
rps4-psbM_p3	rps4-psbM	3	CTGTTGACCCCTGAGCATTT	CAAGTGGTAAGGCAGAGGAC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.185464	16877	17204	1045-1076	1.000000	85.5
rps4-psbM_p4	rps4-psbM	4	CAAATCCCTCTCTTTCCGCA	AAGTGGTAAGGCAGAGGACT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.145355	16877	17204	1492-1528	1.000000	84.3
rps4-psbM_p5	rps4-psbM	5	CTGTTGACCCCTGAGCATTT	CTGGGTGTCGCCTACTTTTT	20	20	50.0	50.0	58.1	58.1	0.0	1.7	0.192650	16877	17204	997-1028	0.971429	83.9
rpoC2-rps2_p1	rpoC2-rps2	1	GCCGGTACTGGAAAATGGAA	TGATGTCCGAGATGAACTGC	20	20	50.0	50.0	58.2	57.7	25.6	0.0	0.471014	27576	27707	197-280	0.971429	86.7
rpoC2-rps2_p2	rpoC2-rps2	2	GCCGGTACTGGAAAATGGAA	GATGTCCGAGATGAACTGCT	20	20	50.0	50.0	58.2	57.4	25.6	0.0	0.764367	27576	27707	196-279	0.971429	84.4
rpoC2-rps2_p3	rpoC2-rps2	3	AGTAGTAAAATACCCGCCGG	TGATGTCCGAGATGAACTGC	20	20	50.0	50.0	57.1	57.7	0.0	0.0	1.196738	27576	27707	194-295	1.000000	82.3
rpoC2-rps2_p4	rpoC2-rps2	4	GCCGGTACTGGAAAATGGAA	TGTCCGAGATGAACTGCTAC	20	20	50.0	50.0	58.2	57.1	25.6	0.0	1.112474	27576	27707	194-277	0.971429	81.7
rpoC2-rps2_p5	rpoC2-rps2	5	GCCGGTACTGGAAAATGGAA	GTCCGAGATGAACTGCTACT	20	20	50.0	50.0	58.2	56.8	25.6	0.0	1.408531	27576	27707	193-276	0.971429	79.3
rps2_p1	rps2	1	AGAAAAGTCATAGATTCCGTAG	GCTTTGCGCTTACTTCGAAA	22	20	36.4	45.0	53.1	57.7	0.0	9.7	7.165990	27708	28427	808-808	1.000000	41.3
rps2_p2	rps2	2	CGTAGGAATGGAAATTCTTTTAGAGA	GCTTTGCGCTTACTTCGAAA	26	20	34.6	45.0	57.3	57.7	0.0	9.7	7.028000	27708	28427	791-791	1.000000	41.2
rps2_p3	rps2	3	CGTAGGAATGGAAATTCTTTT	GCTTTGCGCTTACTTCGAAA	21	20	33.3	45.0	52.1	57.7	0.0	9.7	7.194237	27708	28427	791-791	1.000000	41.2
rps2_p4	rps2	4	TCTTTTAGAGAAGAGAAAAGCA	GCTTTGCGCTTACTTCGAAA	22	20	31.8	45.0	53.1	57.7	0.0	9.7	7.245803	27708	28427	776-776	1.000000	41.2
rps2_p5	rps2	5	CGTAGGAATGGAAATTCTTTTAGAG	GCTTTGCGCTTACTTCGAAA	25	20	36.0	45.0	56.0	57.7	0.0	9.7	7.273948	27708	28427	791-791	1.000000	41.2
trnR-UCU-trnS-GCU_p1	trnR-UCU-trnS-GCU	1	AGAAGACCTCTGTCCTATCCA	CTTTGGTCCTCTCAGCCATC	21	20	47.6	55.0	56.9	58.0	0.0	0.0	2.087135	32665	33429	793-856	1.000000	71.2
trnR-UCU-trnS-GCU_p2	trnR-UCU-trnS-GCU	2	AGGTTTAGAAGACCTCTGTCCT	CTTTGGTCCTCTCAGCCATC	22	20	45.5	55.0	57.9	58.0	0.0	0.0	2.170463	32665	33429	799-862	1.000000	70.5
trnR-UCU-trnS-GCU_p3	trnR-UCU-trnS-GCU	3	AGAAGACCTCTGTCCTATCCA	CGGAGAGAGAGGGATTCGAA	21	20	47.6	55.0	56.9	58.3	0.0	0.0	2.370066	32665	33429	857-920	1.000000	68.8
trnR-UCU-trnS-GCU_p4	trnR-UCU-trnS-GCU	4	AGAAGACCTCTGTCCTATCCA	GAACCCTCGATACGGGATTG	21	20	47.6	55.0	56.9	57.8	0.0	14.8	2.202002	32665	33429	840-903	0.942857	67.7
trnR-UCU-trnS-GCU_p5	trnR-UCU-trnS-GCU	5	AGGTTTAGAAGACCTCTGTCCT	GAACCCTCGATACGGGATTG	22	20	45.5	55.0	57.9	57.8	0.0	14.8	2.285330	32665	33429	846-909	0.942857	67.0
trnF-GAA-trnfM-CAU_p1	trnF-GAA-trnfM-CAU	1	CCGGGATAGCTCAGTTGGTA	ACTCTCGCCTTATGAAAGCG	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.662101	35590	35941	471-671	1.000000	78.4
trnF-GAA-trnfM-CAU_p2	trnF-GAA-trnfM-CAU	2	TCAGTTGGTAGAGCAGAGGA	ACTCTCGCCTTATGAAAGCG	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.677017	35590	35941	461-661	1.000000	78.3
trnF-GAA-trnfM-CAU_p3	trnF-GAA-trnfM-CAU	3	TGGTAGAGCAGAGGACTGAA	ACTCTCGCCTTATGAAAGCG	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.677017	35590	35941	456-656	1.000000	78.3
trnF-GAA-trnfM-CAU_p4	trnF-GAA-trnfM-CAU	4	TTGGTAGAGCAGAGGACTGA	ACTCTCGCCTTATGAAAGCG	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.677017	35590	35941	457-657	1.000000	78.3
trnF-GAA-trnfM-CAU_p5	trnF-GAA-trnfM-CAU	5	AGTTGGTAGAGCAGAGGACT	ACTCTCGCCTTATGAAAGCG	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.686239	35590	35941	459-659	1.000000	78.3
trnfM-CAU-atpE_p1	trnfM-CAU-atpE	1	AAGGCGAGAGTCATTGGTTC	ACGCAAATCGAGTTCTAGGC	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.459028	36015	36078	187-210	0.942857	85.8
trnfM-CAU-atpE_p2	trnfM-CAU-atpE	2	AAGGCGAGAGTCATTGGTTC	CGCAAATCGAGTTCTAGGCA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.460177	36015	36078	186-209	0.942857	85.8
trnfM-CAU-atpE_p3	trnfM-CAU-atpE	3	CGCTTTCATAAGGCGAGAGT	ACGCAAATCGAGTTCTAGGC	20	20	50.0	50.0	58.1	58.4	10.2	0.0	0.430528	36015	36078	196-219	0.942857	85.7
trnfM-CAU-atpE_p4	trnfM-CAU-atpE	4	CGCTTTCATAAGGCGAGAGT	CGCAAATCGAGTTCTAGGCA	20	20	50.0	50.0	58.1	58.4	10.2	0.0	0.431677	36015	36078	195-218	0.942857	85.7
trnfM-CAU-atpE_p5	trnfM-CAU-atpE	5	GGCGAGAGTCATTGGTTCAA	ACGCAAATCGAGTTCTAGGC	20	20	50.0	50.0	58.2	58.4	0.0	0.0	0.550012	36015	36078	185-208	0.942857	85.1
psbJ-psbL_p1	psbJ-psbL	1	TTCTCCCGGTAGTATCAGCC	CAGCCTATACTGGGGATTGT	20	20	55.0	50.0	58.3	56.4	0.0	0.0	1.927909	43036	43160	212-226	1.000000	72.6
psbJ-psbL_p2	psbJ-psbL	2	CTCCCGGTAGTATCAGCCAT	CAGCCTATACTGGGGATTGT	20	20	55.0	50.0	58.4	56.4	0.0	0.0	1.995090	43036	43160	210-224	1.000000	72.1
psbJ-psbL_p3	psbJ-psbL	3	TTCTCCCGGTAGTATCAGCC	TCCGAACGAACAAAGTGTAGA	20	21	55.0	42.9	58.3	57.3	0.0	0.0	2.012903	43036	43160	245-259	1.000000	71.4
psbJ-psbL_p4	psbJ-psbL	4	CTCCCGGTAGTATCAGCCAT	TCCGAACGAACAAAGTGTAGA	20	21	55.0	42.9	58.4	57.3	0.0	0.0	2.080085	43036	43160	243-257	1.000000	70.9
psbJ-psbL_p5	psbJ-psbL	5	TTCTCCCGGTAGTATCAGCC	TGACACAATCAAATCCGAACG	20	21	55.0	42.9	58.3	57.2	0.0	0.0	2.103563	43036	43160	258-272	1.000000	70.4
psaJ-rpl33_p1	psaJ-rpl33	1	TTTCGACAGCACCCGTTTTA	TGCGCCTATGTTTTCTGACA	20	20	45.0	45.0	58.0	57.8	0.0	4.2	0.226566	44910	45033	242-258	1.000000	86.7
psaJ-rpl33_p2	psaJ-rpl33	2	TTTCGACAGCACCCGTTTTA	AAAAATTACCACTCGTGCGC	20	20	45.0	45.0	58.0	57.7	0.0	0.0	0.374681	44910	45033	257-273	1.000000	85.3
psaJ-rpl33_p3	psaJ-rpl33	3	ATATCTTTCGACAGCACCCG	TGCGCCTATGTTTTCTGACA	20	20	50.0	45.0	57.8	57.8	0.0	4.2	0.402462	44910	45033	247-263	1.000000	85.2
psaJ-rpl33_p4	psaJ-rpl33	4	ATATCTTTCGACAGCACCCG	AAAAATTACCACTCGTGCGC	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.550578	44910	45033	262-278	1.000000	83.8
psaJ-rpl33_p5	psaJ-rpl33	5	AGCACCCGTTTTAGCAACTT	TGCGCCTATGTTTTCTGACA	20	20	45.0	45.0	58.3	57.8	0.0	4.2	0.489831	44910	45033	235-251	0.942857	82.1
clpP-psbB_p1	clpP-psbB	1	TCAACGGGTTGCCCTAAAAA	GCTCCGAATCCAAAGCAAAC	20	20	45.0	50.0	57.9	58.0	6.7	0.0	0.086222	47520	48695	1671-1724	1.000000	85.1
clpP-psbB_p2	clpP-psbB	2	TGGTTCCCACATTGGAAGTG	GCTCCGAATCCAAAGCAAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023432	47520	48695	2164-2217	1.000000	84.7
clpP-psbB_p3	clpP-psbB	3	TGGTTCCCACATTGGAAGTG	ATGATGCGACGCTATTCCTC	20	20	50.0	50.0	58.0	57.9	0.0	22.1	0.077732	47520	48695	2306-2359	1.000000	84.0
clpP-psbB_p4	clpP-psbB	4	GGCCAAGACCGATTGTATGT	GCTCCGAATCCAAAGCAAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.055775	47520	48695	1856-1909	0.971429	83.8
clpP-psbB_p5	clpP-psbB	5	AACATGGTTCGGGATTAGCC	GCTCCGAATCCAAAGCAAAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070170	47520	48695	2236-2289	0.971429	83.0
rps11-rpl36_p1	rps11-rpl36	1	TCCACATAGATTTTGGTTTCA	TCGTAGAGGAAAGCGAATTT	21	20	33.3	40.0	53.0	54.8	0.0	0.0	9.172436	54630	54712	169-186	1.000000	47.8
rps11-rpl36_p2	rps11-rpl36	2	TCCACATAGATTTTGGTTTCA	TTCGTAGAGGAAAGCGAATT	21	20	33.3	40.0	53.0	54.8	0.0	0.0	9.172436	54630	54712	170-187	1.000000	47.8
rps11-rpl36_p3	rps11-rpl36	3	TTCCACATAGATTTTGGTTTCA	TCGTAGAGGAAAGCGAATTT	22	20	31.8	40.0	53.9	54.8	0.0	0.0	9.288625	54630	54712	170-187	1.000000	47.8
rps11-rpl36_p4	rps11-rpl36	4	TCCACATAGATTTTGGTTTCA	AATTCGTAGAGGAAAGCGAA	21	20	33.3	40.0	53.0	54.8	0.0	0.0	9.172436	54630	54712	172-189	1.000000	47.7
rps11-rpl36_p5	rps11-rpl36	5	TTCCACATAGATTTTGGTTTCA	TTCGTAGAGGAAAGCGAATT	22	20	31.8	40.0	53.9	54.8	0.0	0.0	9.288625	54630	54712	171-188	1.000000	47.7
rpl2_p1	rpl2	1	GAGTGTGTAGCCGCAAAAAC	ACCAGATACCTACTACGGGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.039877	58726	60001	1552-1639	1.000000	87.0
rpl2_p2	rpl2	2	GAGTGTGTAGCCGCAAAAAC	CAGATACCTACTACGGGCCA	20	20	50.0	55.0	58.0	58.0	0.0	4.4	0.041293	58726	60001	1550-1637	1.000000	87.0
rpl2_p3	rpl2	3	GAGTGTGTAGCCGCAAAAAC	TCCATTCAAGTTGAACGCCA	20	20	50.0	45.0	58.0	58.0	0.0	20.3	0.058149	58726	60001	1596-1683	1.000000	86.8
rpl2_p4	rpl2	4	GAGTGTGTAGCCGCAAAAAC	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	58.0	58.0	0.0	2.3	0.041615	58726	60001	1972-2059	1.000000	86.3
rpl2_p5	rpl2	5	AAGGAACGTCCCTTAGCTCT	ACCAGATACCTACTACGGGC	20	20	50.0	55.0	58.1	58.0	3.3	0.0	0.067482	58726	60001	2166-2253	1.000000	86.1
rps15-psaC_p1	rps15-psaC	1	CAAAGGAGTCTGCGGAAACT	AATTTTGACTGAATGAGCCA	20	20	50.0	35.0	58.1	53.3	0.0	0.0	4.770586	80732	81037	400-454	1.000000	53.8
rps15-psaC_p2	rps15-psaC	2	TGATCAAACGGAAGCGACTT	AATTTTGACTGAATGAGCCA	20	20	45.0	35.0	57.8	53.3	0.0	0.0	4.818639	80732	81037	378-432	1.000000	53.5
rps15-psaC_p3	rps15-psaC	3	TTGATCAAACGGAAGCGACT	AATTTTGACTGAATGAGCCA	20	20	45.0	35.0	57.8	53.3	5.4	0.0	4.818639	80732	81037	379-433	1.000000	53.5
rps15-psaC_p4	rps15-psaC	4	CAAAGGAGTCTGCGGAAACT	GTACAACCAATGCATGTATCA	20	21	50.0	38.1	58.1	54.3	0.0	0.0	4.847557	80732	81037	423-477	1.000000	53.2
rps15-psaC_p5	rps15-psaC	5	CAAAGGAGTCTGCGGAAACT	AAATTTTGACTGAATGAGCCA	20	21	50.0	33.3	58.1	54.2	0.0	0.0	4.868013	80732	81037	401-455	1.000000	53.1
psaC_p1	psaC	1	TAGATCGGGCTACTCTGGTG	TCGTTGGTTGGGTGCAATTA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.034680	81038	81283	1644-1686	1.000000	88.1
psaC_p2	psaC	2	CAATGCAAGAGACGTGCTTG	TCGTTGGTTGGGTGCAATTA	20	20	50.0	45.0	58.0	58.0	18.6	0.0	0.057593	81038	81283	1709-1751	1.000000	87.8
psaC_p3	psaC	3	TAGATCGGGCTACTCTGGTG	CGTTGGTTGGGTGCAATTAC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.076253	81038	81283	1643-1685	1.000000	87.8
psaC_p4	psaC	4	TAGATCGGGCTACTCTGGTG	TATTCGTTGGTTGGGTGCAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.034680	81038	81283	1647-1689	0.971429	86.9
psaC_p5	psaC	5	CAATGCAAGAGACGTGCTTG	TATTCGTTGGTTGGGTGCAA	20	20	50.0	45.0	58.0	58.0	18.6	0.0	0.057593	81038	81283	1712-1754	0.971429	86.5
psaC-ccsA_p1	psaC-ccsA	1	ACCACTCGTAGTATGGGACT	TGTTTGGATATGCTATTTTGGTGT	20	24	50.0	33.3	57.2	57.2	0.0	0.0	5.671793	81284	81389	205-235	1.000000	50.4
psaC-ccsA_p2	psaC-ccsA	2	AACCACTCGTAGTATGGGACT	TGTTTGGATATGCTATTTTGGTGT	21	24	47.6	33.3	57.9	57.2	0.0	0.0	5.946427	81284	81389	206-236	1.000000	50.3
psaC-ccsA_p3	psaC-ccsA	3	CGAAACCACTCGTAGTATGGG	TGTTTGGATATGCTATTTTGGTGT	21	24	52.4	33.3	58.2	57.2	0.0	0.0	6.043241	81284	81389	209-239	1.000000	50.3
psaC-ccsA_p4	psaC-ccsA	4	ACCACTCGTAGTATGGGACT	TTGTTTGGATATGCTATTTTGGTGT	20	25	50.0	32.0	57.2	57.8	0.0	0.0	6.061193	81284	81389	206-236	1.000000	50.3
psaC-ccsA_p5	psaC-ccsA	5	ACCACTCGTAGTATGGGACT	TGTTTGGATATGCTATTTTGGTGTT	20	25	50.0	32.0	57.2	57.8	0.0	0.0	6.061193	81284	81389	215-215	0.085714	4.5
trnL-AUG-ycf1_p1	trnL-AUG-ycf1	1	TTGCACCCAACCAACGAATA	CGGCTGATGGAAGTCGTAAT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.242408	82455	82850	879-914	1.000000	86.1
trnL-AUG-ycf1_p2	trnL-AUG-ycf1	2	TAATTGCACCCAACCAACGA	CGGCTGATGGAAGTCGTAAT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.242408	82455	82850	882-917	1.000000	86.0
trnL-AUG-ycf1_p3	trnL-AUG-ycf1	3	AGAGCAGCGTGTCTACCTAT	CGGCTGATGGAAGTCGTAAT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.285118	82455	82850	468-504	0.971429	85.9
trnL-AUG-ycf1_p4	trnL-AUG-ycf1	4	GTAATTGCACCCAACCAACG	CGGCTGATGGAAGTCGTAAT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.283982	82455	82850	883-918	1.000000	85.7
trnL-AUG-ycf1_p5	trnL-AUG-ycf1	5	CCGTGAGTAAACATAGCGCT	CGGCTGATGGAAGTCGTAAT	20	20	50.0	50.0	58.1	57.8	17.3	0.0	0.302498	82455	82850	910-941	0.771429	75.3