primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ndhF	1	CTAGCACTGGGTAGACCTCA	AGGTAAAATCCGGTGAGTCG	20	20	55.0	50.0	57.9	57.3	0.0	0.0	0.812870	108748	110970	2642-2642	0.500000	53.5
ndhF_p2	ndhF	2	TCGTTGTGGTCGGACTCTAT	AGGTAAAATCCGGTGAGTCG	20	20	50.0	50.0	58.2	57.3	8.2	0.0	0.837194	108748	110970	2821-2821	0.500000	53.3
ndhF_p3	ndhF	3	ATCAATTCGGTCGTTGTGGT	AGGTAAAATCCGGTGAGTCG	20	20	45.0	50.0	57.8	57.3	0.0	0.0	0.846385	108748	110970	2831-2831	0.500000	53.2
ndhF_p4	ndhF	4	TCTCTTCCTTCTCCGAGCTC	AGGTAAAATCCGGTGAGTCG	20	20	55.0	50.0	58.2	57.3	2.7	0.0	0.909791	108748	110970	2763-2763	0.500000	52.7
ndhF_p5	ndhF	5	TGTTTCGATGCAACAGCAAG	AGGTAAAATCCGGTGAGTCG	20	20	45.0	50.0	57.6	57.3	0.0	0.0	1.071738	108748	110970	2416-2416	0.500000	51.4
ndhF-rpl32_p1	ndhF-rpl32	1	CGCCCACACGATTAGTTACA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.008782	110971	112131	2258-2258	0.500000	59.9
ndhF-rpl32_p2	ndhF-rpl32	2	CGCCCACACGATTAGTTACA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010153	110971	112131	2259-2259	0.500000	59.9
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGTCGGAAAAAGAAG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	17.5	0.0	0.040244	110971	112131	1800-1800	0.500000	59.7
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	17.5	0.0	0.041615	110971	112131	1801-1801	0.500000	59.7
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.0	58.0	17.5	0.0	0.051534	110971	112131	1798-1798	0.500000	59.6
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	112830	113801	1578-1578	0.500000	59.6
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	112830	113801	1577-1577	0.500000	59.6
ccsA_p3	ccsA	3	GTGTGGTGGGGAGTCTTTTT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103619	112830	113801	1704-1704	0.500000	59.2
ccsA_p4	ccsA	4	GTGAAATCGGTAGACACGCT	GCATTACCGGGTATGAGTGG	20	20	50.0	55.0	58.0	58.1	0.0	18.9	0.128877	112830	113801	1779-1779	0.500000	59.0
ccsA_p5	ccsA	5	TGAAATCGGTAGACACGCTG	GCATTACCGGGTATGAGTGG	20	20	50.0	55.0	58.0	58.1	0.0	18.9	0.130248	112830	113801	1778-1778	0.500000	59.0
ndhD_p1	ndhD	1	GGGCTCATATTGGAGTTGGG	GGGACGGATGTAAAGCCAAA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.184138	114087	115607	2282-2282	0.500000	58.5
ndhD_p2	ndhD	2	GGGGCTCATATTGGAGTTGG	GGGACGGATGTAAAGCCAAA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.184138	114087	115607	2283-2283	0.500000	58.5
ndhD_p3	ndhD	3	GGGCTCATATTGGAGTTGGG	TGGCACTGTCAATCCAGAAG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.202711	114087	115607	2513-2513	0.500000	58.4
ndhD_p4	ndhD	4	GGGGCTCATATTGGAGTTGG	TGGCACTGTCAATCCAGAAG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.202711	114087	115607	2514-2514	0.500000	58.4
ndhD_p5	ndhD	5	GGGCTCATATTGGAGTTGGG	GGCACTGTCAATCCAGAAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.204245	114087	115607	2512-2512	0.500000	58.4
psaC_p1	psaC	1	TTTCTTTCCTCCCCACATGC	TGGCACTGTCAATCCAGAAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.266238	115703	115948	976-976	0.500000	57.9
psaC_p2	psaC	2	TTTCTTTCCTCCCCACATGC	GGCACTGTCAATCCAGAAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.267772	115703	115948	975-975	0.500000	57.9
psaC_p3	psaC	3	GAGACGTTTCTTTCCTCCCC	TGGCACTGTCAATCCAGAAG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.285814	115703	115948	982-982	0.500000	57.7
psaC_p4	psaC	4	GAGACGTTTCTTTCCTCCCC	GGCACTGTCAATCCAGAAGT	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.287348	115703	115948	981-981	0.500000	57.7
psaC_p5	psaC	5	TCCTCCCCACATGCATAGAA	TGGCACTGTCAATCCAGAAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.297661	115703	115948	970-970	0.500000	57.6
ndhE-ndhG_p1	ndhE-ndhG	1	TTTGGCTTTACATCCGTCCC	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.176391	116503	116734	1138-1138	0.500000	58.6
ndhE-ndhG_p2	ndhE-ndhG	2	CTTCTGGATTGACAGTGCCA	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.194964	116503	116734	907-907	0.500000	58.4
ndhE-ndhG_p3	ndhE-ndhG	3	ACTTCTGGATTGACAGTGCC	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.196498	116503	116734	908-908	0.500000	58.4
ndhE-ndhG_p4	ndhE-ndhG	4	ACATCCGTCCCATGGTATCA	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.2	58.0	11.7	0.0	0.204128	116503	116734	1129-1129	0.500000	58.4
ndhE-ndhG_p5	ndhE-ndhG	5	GGACACATTGAGTACACCCG	TGGAGTAACTTCCGTGGTCT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.283359	116503	116734	1082-1082	0.500000	57.7
ndhG_p1	ndhG	1	CTTCTGGATTGACAGTGCCA	ATTTGCCCGTTGTTGATTGG	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.643577	116735	117265	1919-1919	0.500000	54.9
ndhG_p2	ndhG	2	ACTTCTGGATTGACAGTGCC	ATTTGCCCGTTGTTGATTGG	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.645111	116735	117265	1920-1920	0.500000	54.8
ndhG_p3	ndhG	3	CTTCTGGATTGACAGTGCCA	TGCCCGTTGTTGATTGGAAA	20	20	50.0	45.0	57.8	58.6	0.0	0.0	0.785780	116735	117265	1916-1916	0.500000	53.7
ndhG_p4	ndhG	4	CTTCTGGATTGACAGTGCCA	TTGCCCGTTGTTGATTGGAA	20	20	50.0	45.0	57.8	58.6	0.0	0.0	0.785780	116735	117265	1917-1917	0.500000	53.7
ndhG_p5	ndhG	5	CTTCTGGATTGACAGTGCCA	TTTGCCCGTTGTTGATTGGA	20	20	50.0	45.0	57.8	58.6	0.0	0.0	0.785780	116735	117265	1918-1918	0.500000	53.7
ndhG-ndhI_p1	ndhG-ndhI	1	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064045	117266	117715	1458-1458	0.500000	59.5
ndhG-ndhI_p2	ndhG-ndhI	2	AGACCACGGAAGTTACTCCA	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.098676	117266	117715	1123-1123	0.500000	59.2
ndhG-ndhI_p3	ndhG-ndhI	3	AGACCACGGAAGTTACTCCA	AGATGGACTTTACCGAGGCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138007	117266	117715	1459-1459	0.500000	58.9
ndhG-ndhI_p4	ndhG-ndhI	4	AGACCACGGAAGTTACTCCA	TTACCGAGGCTGAGAATGGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144729	117266	117715	1450-1450	0.500000	58.8
ndhG-ndhI_p5	ndhG-ndhI	5	AGACCACGGAAGTTACTCCA	ATTGATCACATCCGAGCGTT	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.321295	117266	117715	1124-1124	0.500000	57.4
ndhI_p1	ndhI	1	AGTTACTCCATCTCCCACGG	AGCCGAGTGTGAACGATTAC	20	20	55.0	50.0	58.5	58.0	0.0	0.0	0.521619	117716	118216	1753-1753	0.500000	55.8
ndhI_p2	ndhI	2	TTACTCCATCTCCCACGGTC	AGCCGAGTGTGAACGATTAC	20	20	55.0	50.0	58.5	58.0	0.0	0.0	0.525959	117716	118216	1751-1751	0.500000	55.8
ndhI_p3	ndhI	3	AGTTACTCCATCTCCCACGG	GGTTCTGCAATAGGGATGGG	20	20	55.0	55.0	58.5	58.0	0.0	0.0	0.526907	117716	118216	1915-1915	0.500000	55.8
ndhI_p4	ndhI	4	TTACTCCATCTCCCACGGTC	GGTTCTGCAATAGGGATGGG	20	20	55.0	55.0	58.5	58.0	0.0	0.0	0.531247	117716	118216	1913-1913	0.500000	55.8
ndhI_p5	ndhI	5	AGTTACTCCATCTCCCACGG	TTTGTGACCTTTGAGGAGCC	20	20	55.0	50.0	58.5	58.0	0.0	0.1	0.532532	117716	118216	1959-1959	0.500000	55.7
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	AGAGTTGAAGCACCAAAGGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051324	118295	120492	2546-2546	0.500000	59.6
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	AGTTGAAGCACCAAAGGGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051324	118295	120492	2544-2544	0.500000	59.6
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	ACCAATGCTACGAGCTTCTG	20	20	50.0	50.0	58.0	58.0	0.0	8.1	0.053942	118295	120492	2868-2868	0.500000	59.6
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	GAGTTGAAGCACCAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056950	118295	120492	2545-2545	0.500000	59.5
ndhA_p5	ndhA	5	TGACCAATATACCTTGCGGC	GTTGAAGCACCAAAGGGAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056950	118295	120492	2543-2543	0.500000	59.5
ndhH_p1	ndhH	1	CGTTGTTGTATACCCGCAGA	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.100714	120494	121672	1543-1543	0.500000	59.2
ndhH_p2	ndhH	2	CTGCAAATTTCGAAGCCGTT	CGCTTGCTGGCTTATTTGTC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.150240	120494	121672	2074-2074	0.500000	58.8
ndhH_p3	ndhH	3	CCAAAGGTCCTGCGTATTCA	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.207713	120494	121672	1572-1572	0.500000	58.3
ndhH_p4	ndhH	4	CGTTGTTGTATACCCGCAGA	AATAATGGGAGCCGTTTGGG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.225567	120494	121672	1977-1977	0.500000	58.2
ndhH_p5	ndhH	5	ACCATCTGCTAGAGCTTGGA	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.1	58.1	19.1	0.0	0.228345	120494	121672	1598-1598	0.500000	58.2
ndhH-rps15_p1	ndhH-rps15	1	TGAGGTAAATCAGCAGCGAC	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109908	121673	121763	684-684	0.500000	59.1
ndhH-rps15_p2	ndhH-rps15	2	CAGAAGCTCGTAGCATTGGT	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.0	58.1	9.1	0.0	0.115499	121673	121763	860-860	0.500000	59.1
ndhH-rps15_p3	ndhH-rps15	3	AACACCATGCATTGATGGGT	CGCTTGCTGGCTTATTTGTC	20	20	45.0	50.0	58.1	58.1	8.9	0.0	0.158386	121673	121763	253-253	0.500000	58.7
ndhH-rps15_p4	ndhH-rps15	4	CCTAATTGCTCTGGGCCATT	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	57.9	58.1	6.7	0.0	0.177450	121673	121763	447-447	0.500000	58.6
ndhH-rps15_p5	ndhH-rps15	5	TGGGATTCCGCGTAATAAGC	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.1	58.1	4.9	0.0	0.226065	121673	121763	770-770	0.500000	58.2
rps15_p1	rps15	1	TGAGGTAAATCAGCAGCGAC	AATAATGGGAGCCGTTTGGG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.234760	121764	122033	1118-1118	0.500000	58.1
rps15_p2	rps15	2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGTTTGGG	20	20	45.0	50.0	58.1	58.2	8.9	0.0	0.283238	121764	122033	687-687	0.500000	57.7
rps15_p3	rps15	3	CCTAATTGCTCTGGGCCATT	AATAATGGGAGCCGTTTGGG	20	20	50.0	50.0	57.9	58.2	6.7	0.0	0.302302	121764	122033	881-881	0.500000	57.6
rps15_p4	rps15	4	TGGGATTCCGCGTAATAAGC	AATAATGGGAGCCGTTTGGG	20	20	50.0	50.0	58.1	58.2	4.9	0.0	0.350917	121764	122033	1204-1204	0.500000	57.2
rps15_p5	rps15	5	GCTCTGGGCCATTTACAGTT	AATAATGGGAGCCGTTTGGG	20	20	50.0	50.0	58.2	58.2	6.7	0.0	0.385191	121764	122033	874-874	0.500000	56.9
ycf1_p1	ycf1	1	TCGAGGAGGACCAAAAGGAT	TATTTGGCGACCGTCTCAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051961	124779	124898	604-604	0.500000	59.6
ycf1_p2	ycf1	2	TCGAGGAGGACCAAAAGGAT	AGAACCGGATTTTCGTCGAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.055209	124779	124898	667-667	0.500000	59.6
ycf1_p3	ycf1	3	TCGAGGAGGACCAAAAGGAT	GACCGTCTCAAGGAAATCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.072967	124779	124898	596-596	0.500000	59.4
ycf1_p4	ycf1	4	TCGAGGAGGACCAAAAGGAT	TAATGCAAGGGCTCGATCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.093820	124779	124898	821-821	0.500000	59.2
ycf1_p5	ycf1	5	TCGAGGAGGACCAAAAGGAT	TTAACTCGTTCCAGCCGAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.121926	124779	124898	765-765	0.500000	59.0