primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	85	412	880-1029	0.968750	89.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	85	412	894-1043	0.968750	89.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	85	412	893-1042	0.968750	89.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	85	412	895-1044	0.968750	89.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	AAGCTCCAACAAACGGCTAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.153260	85	412	228-377	0.750000	80.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAGGTAGAAGATTCGGGGGT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	57.7	57.9	0.0	18.8	0.341505	4286	4800	1506-1549	0.968750	87.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGGTAGAAGATTCGGGGGTT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	57.7	57.9	0.0	18.8	0.341505	4286	4800	1505-1548	0.968750	87.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.3	57.9	0.0	18.8	0.341816	4286	4800	854-875	0.968750	86.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGGTAGAAGATTCGGGGGT	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.353631	4286	4800	1450-1493	0.781250	79.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGGTAGAAGATTCGGGGGTT	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.353631	4286	4800	1449-1492	0.781250	79.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5948	6763	769-1075	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	20	20	45.0	55.0	58.1	57.9	8.1	0.0	0.261887	5948	6763	782-1088	1.000000	89.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCTCGCTATT	20	20	45.0	50.0	58.1	58.3	8.1	0.0	0.405161	5948	6763	791-1004	0.906250	85.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAAAATCCACAACGGGTCGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.3	58.1	0.0	16.9	0.393361	5948	6763	796-1009	0.875000	83.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAAAATCCACAACGGGTCGT	GTCTCGCTATTCGGAGGTTC	20	20	45.0	55.0	58.3	57.9	0.0	0.0	0.439823	5948	6763	809-1022	0.875000	83.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034421	8127	8797	1298-1482	1.000000	91.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035809	8127	8797	1297-1481	1.000000	91.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	TAGTCGACGCCAATTCAGTG	20	20	50.0	50.0	58.0	58.0	0.0	10.5	0.013783	8127	8797	1270-1430	0.687500	79.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	TAGTCGACGCCAATTCAGTG	20	20	50.0	50.0	58.0	58.0	0.0	10.5	0.015171	8127	8797	1269-1429	0.687500	79.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CCTCGCCTTTGAGCCTTTAT	TAGTCGACGCCAATTCAGTG	20	20	50.0	50.0	58.0	58.0	0.0	10.5	0.053324	8127	8797	1812-1972	0.687500	78.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGAGCCCATATCCTTGCTTT	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032498	26303	27446	1833-2010	0.750000	81.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032498	26303	27446	1831-2008	0.750000	81.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTGTTTCCTACTCACGCGAG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	57.9	58.0	15.9	0.0	0.065696	26303	27446	1847-2024	0.750000	81.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCGCCTACACAAGCAAATTG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024117	26303	27446	2025-2202	0.750000	80.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGCCTACACAAGCAAATTG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024117	26303	27446	2023-2200	0.750000	80.9
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	CCCTTATTTCCCCGGGATTG	20	20	50.0	55.0	58.0	58.0	0.0	26.3	0.009994	27770	28769	1250-1806	1.000000	91.9
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	CCCTTATTTCCCCGGGATTG	20	20	50.0	55.0	58.0	58.0	0.0	26.3	0.009994	27770	28769	1248-1804	1.000000	91.9
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTACCGACCGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053814	27770	28769	593-1154	1.000000	91.6
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTACCGACCGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053814	27770	28769	591-1152	1.000000	91.6
petN-psbM_p5	petN-psbM	5	TCCGGGAAAAATTGCATCCA	CCCTTATTTCCCCGGGATTG	20	20	45.0	55.0	58.1	58.0	0.0	26.3	0.074373	27770	28769	1576-2132	0.906250	87.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	30285	31401	867-1443	1.000000	90.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	30285	31401	870-1446	1.000000	90.4
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	30285	31401	857-1433	1.000000	89.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	30285	31401	860-1436	1.000000	89.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	30285	31401	844-1420	1.000000	88.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	45277	45948	702-786	1.000000	89.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	45277	45948	734-818	1.000000	88.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	45277	45948	696-780	1.000000	87.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	45277	45948	734-818	1.000000	86.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	45277	45948	728-812	1.000000	86.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGGGGCTAAAATCCCAGAAA	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.1	0.0	0.821159	49261	50195	959-1080	1.000000	85.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGGGCTAAAATCCCAGAAA	TTCGAGTCCGTATAGCCCTA	20	20	50.0	50.0	57.9	57.1	0.1	0.0	1.074729	49261	50195	958-1079	1.000000	83.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCCGGCCCTTTTCTAATCG	GTTCGAGTCCGTATAGCCCT	20	20	55.0	55.0	58.4	58.7	0.0	0.0	1.100296	49261	50195	977-1098	1.000000	83.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AATCGGGGCTAAAATCCCAG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.6	58.7	0.1	0.0	1.048321	49261	50195	962-1083	0.937500	81.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AATCGGGGCTAAAATCCCAG	TTCGAGTCCGTATAGCCCTA	20	20	50.0	50.0	57.6	57.1	0.1	0.0	1.301892	49261	50195	961-1082	0.937500	79.1
ycf4-cemA_p1	ycf4-cemA	1	TTAGCCTATTTCTTGCGCGT	GAAATCCACCAGGGCAGAAA	20	20	45.0	50.0	58.0	58.1	8.4	0.0	0.095186	59458	60140	768-802	1.000000	91.2
ycf4-cemA_p2	ycf4-cemA	2	TGCGCGTACCAATTGAAGTA	GAAATCCACCAGGGCAGAAA	20	20	45.0	50.0	57.9	58.1	8.4	0.0	0.158370	59458	60140	755-789	1.000000	90.7
ycf4-cemA_p3	ycf4-cemA	3	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	59458	60140	757-791	1.000000	90.4
ycf4-cemA_p4	ycf4-cemA	4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	20	20	45.0	50.0	57.7	58.1	8.4	0.0	0.400584	59458	60140	760-794	0.968750	87.1
ycf4-cemA_p5	ycf4-cemA	5	CCTATTTCTTGCGCGTACCA	GAAATCCACCAGGGCAGAAA	20	20	50.0	50.0	58.4	58.1	8.4	0.0	0.429298	59458	60140	764-798	0.968750	86.8
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	ACCGAACTGAACTAAGAGCG	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.7	57.9	0.0	8.4	0.463208	65291	65453	204-267	1.000000	88.3
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	ACCGAACTGAACTAAGAGCG	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.7	58.2	0.0	8.4	0.520466	65291	65453	203-266	1.000000	87.8
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	ACCGAACTGAACTAAGAGCG	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.7	57.8	0.0	0.0	0.539622	65291	65453	196-259	1.000000	87.7
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCGAACTGAACTAAGAGCG	TAGCGCGTTTGTTTTGGGTA	20	20	50.0	45.0	57.7	58.4	0.0	8.4	0.758365	65291	65453	199-262	1.000000	85.9
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	CCGAACTGAACTAAGAGCGC	CTTGGTAGCGCGTTTGTTTT	20	20	55.0	45.0	59.0	57.9	0.0	8.4	1.121109	65291	65453	203-266	1.000000	83.0
ndhF-rpl32_p1	ndhF-rpl32	1	CACCCATATTGATATGTCTGTTGC	TGACCCAGAAGTACGTTTTT	24	20	41.7	40.0	58.0	54.8	9.5	0.0	7.173726	110488	110888	448-479	0.906250	48.3
ndhF-rpl32_p2	ndhF-rpl32	2	CACCCATATTGATATGTCTGTTGC	TTGACCCAGAAGTACGTTTT	24	20	41.7	40.0	58.0	54.8	9.5	0.0	7.173726	110488	110888	449-480	0.906250	48.3
ndhF-rpl32_p3	ndhF-rpl32	3	CACCCATATTGATATGTCTGTTGC	TTTGACCCAGAAGTACGTTT	24	20	41.7	40.0	58.0	54.8	9.5	0.0	7.173726	110488	110888	450-481	0.906250	48.3
ndhF-rpl32_p4	ndhF-rpl32	4	CACCCATATTGATATGTCTGTTGC	TTTTGACCCAGAAGTACGTT	24	20	41.7	40.0	58.0	54.8	9.5	0.0	7.173726	110488	110888	451-482	0.906250	48.3
ndhF-rpl32_p5	ndhF-rpl32	5	CACCCATATTGATATGTCTGTTGC	TTTTTGACCCAGAAGTACGT	24	20	41.7	40.0	58.0	54.8	9.5	0.0	7.173726	110488	110888	452-483	0.906250	48.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGAAAGGCTATTCGGCAG	GGTAGCAAAACCCTGGGAAA	20	20	50.0	50.0	58.0	58.0	0.0	22.2	0.048060	111063	111752	1261-1321	0.656250	77.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGGAAAGGCTATTCGGCAG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.168400	111063	111752	791-842	0.656250	76.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGAAAGGCTATTCGGCAG	AAGACCCGAGGTAGCAAAAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.224555	111063	111752	1270-1330	0.656250	76.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGAAAGGCTATTCGGCAG	ATGCCCCTTTCGGATGAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057240	111063	111752	1053-1113	0.593750	75.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGAAAGGCTATTCGGCAG	GCCCCTTTCGGATGAATCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057240	111063	111752	1051-1111	0.593750	75.3
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAACGGTCCAAAGATGAA	ACAAACAATACGAGCTGCCA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.204837	116334	116685	1081-1118	1.000000	90.4
ndhG-ndhI_p2	ndhG-ndhI	2	CCCAACGGTCCAAAGATGAA	ATTAGAACGTTTCCGAGGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.5	0.115661	116334	116685	973-1002	0.843750	84.6
ndhG-ndhI_p3	ndhG-ndhI	3	AGACCCGGTCCTAGAAAGAC	ATTAGAACGTTTCCGAGGCC	20	20	55.0	50.0	58.2	58.0	14.3	0.5	0.169971	116334	116685	741-770	0.843750	84.4
ndhG-ndhI_p4	ndhG-ndhI	4	GACCCGGTCCTAGAAAGACT	ATTAGAACGTTTCCGAGGCC	20	20	55.0	50.0	58.2	58.0	6.3	0.5	0.169971	116334	116685	740-769	0.843750	84.4
ndhG-ndhI_p5	ndhG-ndhI	5	ATGATCCCATACCAGGCTGT	ATTAGAACGTTTCCGAGGCC	20	20	50.0	50.0	58.2	58.0	14.4	0.5	0.194843	116334	116685	1081-1085	0.187500	52.2
ycf1_p1	ycf1	1	TGCGCATTTGCTTGAAACAA	CCTCCCGGTTTAGCCTTTTT	20	20	40.0	50.0	58.1	58.1	5.7	0.0	0.148854	124771	124890	965-965	0.250000	56.6
ycf1_p2	ycf1	2	GCAAGTGGAATGCTATCCCA	CCTCCCGGTTTAGCCTTTTT	20	20	50.0	50.0	57.9	58.1	6.0	0.0	0.137897	124771	124890	1190-1190	0.250000	55.9
ycf1_p3	ycf1	3	GCAATTGGAGTATACGGCCA	CCTCCCGGTTTAGCCTTTTT	20	20	50.0	50.0	58.0	58.1	14.2	0.0	0.118966	124771	124890	1297-1297	0.218750	52.4
ycf1_p4	ycf1	4	TGCAATTGGAGTATACGGCC	CCTCCCGGTTTAGCCTTTTT	20	20	50.0	50.0	58.0	58.1	7.7	0.0	0.118966	124771	124890	1298-1298	0.218750	52.4
ycf1_p5	ycf1	5	GGACCAAAAGGAAGTTCCGT	CCTCCCGGTTTAGCCTTTTT	20	20	50.0	50.0	58.0	58.1	16.3	0.0	0.103704	124771	124890	1571-1571	0.062500	45.2