primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	101	501	519-519	1.000000	76.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	101	501	533-533	1.000000	76.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	101	501	532-532	1.000000	76.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	101	501	534-534	1.000000	76.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	101	501	520-520	1.000000	75.8
psbA_p1	psbA	1	GAGCAATAACGCCCTCTTGA	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.0	58.2	0.0	9.7	0.259325	502	1563	1203-1203	1.000000	77.9
psbA_p2	psbA	2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	57.8	58.2	0.0	9.7	0.451091	502	1563	1208-1208	1.000000	76.4
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTGA	ATATTGGTTGACACGGGCAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	502	1563	1268-1268	1.000000	76.3
psbA_p4	psbA	4	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	502	1563	1266-1266	1.000000	76.3
psbA_p5	psbA	5	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.2	58.2	0.0	9.7	0.483712	502	1563	1207-1207	1.000000	76.1
matK_p1	matK	1	TTCCAAGGTTTCCTAACAAA	ACCCCCAAAATCCTCTACCT	20	20	35.0	50.0	52.5	57.6	0.0	0.0	5.915335	2092	3612	1582-1582	1.000000	40.0
matK_p2	matK	2	TTTCCAAGGTTTCCTAACAA	ACCCCCAAAATCCTCTACCT	20	20	35.0	50.0	52.5	57.6	0.0	0.0	5.915335	2092	3612	1583-1583	1.000000	40.0
matK_p3	matK	3	TTTCCAAGGTTTCCTAACAAA	ACCCCCAAAATCCTCTACCT	21	20	33.3	50.0	53.4	57.6	0.0	0.0	5.961143	2092	3612	1583-1583	1.000000	40.0
matK_p4	matK	4	ATTTCCAAGGTTTCCTAACA	ACCCCCAAAATCCTCTACCT	20	20	35.0	50.0	52.2	57.6	0.0	0.0	6.167427	2092	3612	1584-1584	1.000000	40.0
matK_p5	matK	5	ATTTCCAAGGTTTCCTAACAA	ACCCCCAAAATCCTCTACCT	21	20	33.3	50.0	53.2	57.6	0.0	0.0	6.197473	2092	3612	1584-1584	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3613	4344	864-864	1.000000	58.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3613	4344	863-863	1.000000	58.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3613	4344	879-879	1.000000	56.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	22	20	40.9	50.0	57.5	57.5	32.7	0.0	2.953251	3613	4344	877-877	1.000000	56.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	55.0	58.2	32.7	17.1	3.163567	3613	4344	864-864	1.000000	54.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	CCTTGAAAAAGGTGCTCAGC	20	20	50.0	50.0	58.2	57.6	20.3	0.0	0.597307	4382	5098	863-863	1.000000	75.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	CTTGAAAAAGGTGCTCAGCC	20	20	50.0	50.0	58.2	57.6	20.3	0.0	0.597307	4382	5098	862-862	1.000000	75.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	CCTTGAAAAAGGTGCTCAGC	20	20	50.0	50.0	58.2	57.6	20.3	0.0	0.597307	4382	5098	862-862	1.000000	75.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGTGCTCAGCC	20	20	50.0	50.0	58.2	57.6	20.3	0.0	0.597307	4382	5098	861-861	1.000000	75.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	CTTGAAAAAGGTGCTCAGCC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.663187	4382	5098	947-947	1.000000	74.7
rps16_p1	rps16	1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.9	57.7	0.0	0.0	4.403459	5099	6231	1310-1310	1.000000	44.8
rps16_p2	rps16	2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	58.2	57.7	0.0	0.0	4.486645	5099	6231	1310-1310	1.000000	44.1
rps16_p3	rps16	3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.6	57.7	0.0	0.0	4.654102	5099	6231	1309-1309	1.000000	42.8
rps16_p4	rps16	4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	23	20	39.1	55.0	56.9	57.4	0.0	0.0	4.755119	5099	6231	1311-1311	1.000000	42.0
rps16_p5	rps16	5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	24	20	37.5	55.0	58.2	57.4	0.0	0.0	4.838305	5099	6231	1311-1311	1.000000	41.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6232	7159	1001-1001	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	6232	7159	1031-1031	1.000000	77.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6232	7159	1016-1016	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6232	7159	1015-1015	1.000000	76.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	TAAGGCATCGGGTTTTGGTC	20	20	55.0	50.0	58.4	58.2	2.9	0.0	0.540873	6232	7159	1045-1045	1.000000	75.7
trnS-GGA-trnC-GCA_p1	trnS-GGA-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8492	9252	912-912	1.000000	78.1
trnS-GGA-trnC-GCA_p2	trnS-GGA-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8492	9252	894-894	1.000000	75.9
trnS-GGA-trnC-GCA_p3	trnS-GGA-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8492	9252	848-848	1.000000	74.3
trnS-GGA-trnC-GCA_p4	trnS-GGA-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8492	9252	913-913	1.000000	73.9
trnS-GGA-trnC-GCA_p5	trnS-GGA-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8492	9252	830-830	1.000000	72.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9334	10146	879-879	1.000000	78.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9334	10146	897-897	1.000000	75.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9334	10146	878-878	1.000000	72.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9334	10146	887-887	1.000000	72.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9334	10146	936-936	1.000000	71.5
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	45.0	50.0	55.8	58.3	0.0	0.0	2.491460	10834	11487	765-765	1.000000	60.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	20	20	45.0	50.0	55.8	58.3	0.0	26.3	2.570277	10834	11487	780-780	1.000000	59.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	TTCCCCGGGATTGTAGTTCA	20	20	45.0	50.0	55.8	58.3	0.0	26.3	2.570277	10834	11487	781-781	1.000000	59.4
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	22	20	40.9	50.0	57.4	58.3	0.0	0.0	2.886094	10834	11487	772-772	1.000000	56.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAGAATGAACAGTGCA	TCCCCGGGATTGTAGTTCAA	22	20	40.9	50.0	57.4	58.3	0.0	26.3	2.964910	10834	11487	787-787	1.000000	56.3
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.0	56.8	0.0	0.0	2.237066	11994	12813	962-962	1.000000	62.1
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.1	56.8	14.8	0.0	2.326325	11994	12813	921-921	1.000000	61.4
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	11994	12813	952-952	1.000000	61.3
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	11994	12813	957-957	1.000000	61.3
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.0	57.6	0.0	0.0	2.409970	11994	12813	952-952	1.000000	60.7
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62383	63179	935-935	1.000000	76.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62383	63179	925-925	1.000000	76.8
petA-psbJ_p3	petA-psbJ	3	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.754934	62383	63179	941-941	1.000000	74.0
petA-psbJ_p4	petA-psbJ	4	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.761562	62383	63179	940-940	1.000000	74.0
petA-psbJ_p5	petA-psbJ	5	GCCTTTTGTTCTTCTTCGCA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.2	57.6	0.0	4.1	1.139411	62383	63179	924-924	1.000000	71.0