primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCGGTCTTACAAACTC	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.069982	4282	5056	1545-1676	0.906977	87.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCGCGGTCTTACAAACTCT	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.069982	4282	5056	1544-1675	0.906977	87.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GTCGCGGTCTTACAAACTCT	TTGAAAAAGGTGCTCAGCCT	20	20	50.0	45.0	57.9	57.9	6.1	0.0	0.133517	4282	5056	873-1014	0.906977	87.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.066364	4282	5056	852-977	0.279070	59.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAGGCGGGTGT	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.066364	4282	5056	851-976	0.279070	59.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6268	7032	548-905	0.976744	89.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6268	7032	552-909	0.976744	84.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6268	7032	540-897	0.976744	84.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.9	58.1	8.1	16.9	0.938588	6268	7032	547-904	0.976744	83.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	59.1	58.1	0.0	16.9	1.134559	6268	7032	543-900	0.976744	82.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.241837	8491	9146	408-759	0.953488	80.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.243225	8491	9146	407-758	0.953488	80.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.248863	8491	9146	408-759	0.953488	80.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.250251	8491	9146	407-758	0.953488	80.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	AACGAATCGCACTTTTACCAC	20	21	50.0	42.9	58.0	57.5	0.0	1.2	1.535845	8491	9146	409-760	0.953488	77.9
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GATACCGGGGCTTTGCAATA	ATTACCATCCCCGATTTGCC	20	20	50.0	50.0	58.0	58.0	6.3	0.0	0.028964	26741	27792	2041-2116	0.186047	54.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AGAGGTTCCCAGGGAATTCA	ATTACCATCCCCGATTTGCC	20	20	50.0	50.0	57.9	58.0	24.7	0.0	0.064380	26741	27792	1984-2059	0.186047	54.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCGGGGCTTTGCAATATTTG	ATTACCATCCCCGATTTGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.065998	26741	27792	2037-2112	0.186047	54.0
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ACACGAACCCATATCCTTGC	ATTACCATCCCCGATTTGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043011	26741	27792	2160-2235	0.186047	53.8
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCCTACTCACACGAACCCAT	ATTACCATCCCCGATTTGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071602	26741	27792	2168-2243	0.186047	53.6
petN-psbM_p1	petN-psbM	1	GGCAAATCGGGGATGGTAAT	AATCATCAAACCGCCCTACC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.057406	28893	29640	1702-1795	0.186047	54.9
petN-psbM_p2	petN-psbM	2	GGCAAATCGGGGATGGTAAT	TATAAACTAGTGCTCGCGCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.134447	28893	29640	1751-1844	0.186047	54.3
petN-psbM_p3	petN-psbM	3	ATATGGCAAATCGGGGATGG	AATCATCAAACCGCCCTACC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273435	28893	29640	1786-1799	0.162791	51.8
petN-psbM_p4	petN-psbM	4	GGCAAATCGGGGATGGTAAT	GCCCTACCGTTGCTTCTAAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.108161	28893	29640	1772-1783	0.139535	46.2
petN-psbM_p5	petN-psbM	5	GGCAAATCGGGGATGGTAAT	TCAAATCATCAAACCGCCCT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.219000	28893	29640	1787-1798	0.139535	45.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	ACGCCTTACCATGACGTTAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.145826	30961	31507	342-629	1.000000	90.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	ACGCCTTACCATGACGTTAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.145826	30961	31507	340-627	1.000000	90.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GATGTCCTGAACCGCTAGAC	ACGCCTTACCATGACGTTAC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.211782	30961	31507	339-626	1.000000	90.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30961	31507	355-642	0.953488	88.9
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30961	31507	353-640	0.953488	88.9
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TCTGGTACAACATTATGGATTGGA	AATCGAACCCGCATCTTCTC	24	20	37.5	50.0	57.7	58.1	0.0	0.0	4.379109	36031	36544	598-664	1.000000	57.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCTGGTACAACATTATGGATTGGA	GAATCGAACCCGCATCTTCT	24	20	37.5	50.0	57.7	58.1	0.0	0.0	4.379109	36031	36544	599-665	1.000000	57.0
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCTGGTACAACATTATGGATTGGA	CATCTTCTCCGTGGCAAAGA	24	20	37.5	50.0	57.7	57.9	0.0	1.4	4.419575	36031	36544	587-653	1.000000	56.6
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCTGGTACAACATTATGGATTGGA	CTTCTCCGTGGCAAAGAGAA	24	20	37.5	50.0	57.7	57.8	0.0	22.2	4.490266	36031	36544	584-650	1.000000	56.1
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCTGGTACAACATTATGGATTGGA	ATCTTCTCCGTGGCAAAGAG	24	20	37.5	50.0	57.7	57.6	0.0	8.4	4.713705	36031	36544	586-652	1.000000	54.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TTTTCCGGCGATATGAACGT	TAGCGTCTACCAATTTCGCC	20	20	45.0	50.0	58.0	58.1	9.3	0.0	0.082369	46898	47507	1345-1502	0.976744	90.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TTTTCCGGCGATATGAACGT	AAATGGGCCTTTTTGGGGAT	20	20	45.0	45.0	58.0	57.9	9.3	11.3	0.071188	46898	47507	1929-2085	0.790698	83.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TTTTCCGGCGATATGAACGT	ACCAATTTCGCCATATCCCC	20	20	45.0	50.0	58.0	58.0	9.3	0.0	0.017561	46898	47507	1337-1494	0.325581	61.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TGCTTTTCCGGCGATATGAA	ACCAATTTCGCCATATCCCC	20	20	45.0	50.0	58.0	58.0	9.3	0.0	0.035882	46898	47507	1340-1497	0.325581	61.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TTGCTTTTCCGGCGATATGA	ACCAATTTCGCCATATCCCC	20	20	45.0	50.0	58.0	58.0	9.3	0.0	0.035882	46898	47507	1341-1498	0.325581	61.8
accD-psaI_p1	accD-psaI	1	TGAACCCGATGCCTACATTG	TCGAGACCCCGCTATAAGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.099928	59084	59774	1486-1592	1.000000	91.2
accD-psaI_p2	accD-psaI	2	TGAACCCGATGCCTACATTG	TTCGAGACCCCGCTATAAGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.099928	59084	59774	1487-1593	1.000000	91.2
accD-psaI_p3	accD-psaI	3	CTACTACAGGTGGGGTGACA	TCGAGACCCCGCTATAAGTT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.129949	59084	59774	1541-1647	1.000000	91.0
accD-psaI_p4	accD-psaI	4	TACTACAGGTGGGGTGACAG	TCGAGACCCCGCTATAAGTT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.129949	59084	59774	1540-1646	1.000000	91.0
accD-psaI_p5	accD-psaI	5	TACTACAGGTGGGGTGACAG	TTCGAGACCCCGCTATAAGT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.129949	59084	59774	1541-1647	1.000000	91.0
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	AGGGAAATAAATGCTTTCTTTTT	20	23	45.0	26.1	58.1	53.3	3.3	0.0	7.760056	60908	61648	449-825	1.000000	52.0
ycf4-cemA_p2	ycf4-cemA	2	TGAACAGAAAGCCGCTGAAT	AGGGAAATAAATGCTTTCTTTTT	20	23	45.0	26.1	58.1	53.3	0.0	0.0	7.768123	60908	61648	468-844	1.000000	52.0
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	AGGGAAATAAATGCTTTCTTTT	20	22	45.0	27.3	58.1	52.5	3.3	0.0	7.632688	60908	61648	449-825	0.976744	51.1
ycf4-cemA_p4	ycf4-cemA	4	ATTGAACAGAAAGCCGCTGA	AGGGAAATAAATGCTTTCTTTT	20	22	45.0	27.3	58.1	52.5	0.0	0.0	7.640755	60908	61648	470-846	0.976744	51.1
ycf4-cemA_p5	ycf4-cemA	5	TGAACAGAAAGCCGCTGAAT	AGGGAAATAAATGCTTTCTTTT	20	22	45.0	27.3	58.1	52.5	0.0	0.0	7.640755	60908	61648	468-844	0.976744	51.1
petA-psbJ_p1	petA-psbJ	1	AGAAACAGTTTGAGAAGGTTCA	TTGTGATCGGTTTAATCGGT	22	20	36.4	40.0	55.8	55.1	2.3	0.0	7.125562	63509	64423	713-1082	1.000000	52.0
petA-psbJ_p2	petA-psbJ	2	AGAAACAGTTTGAGAAGGTTCA	TCTGGATTAGGTTCATCCCT	22	20	36.4	45.0	55.8	54.9	2.3	0.0	7.337896	63509	64423	672-1041	1.000000	52.0
petA-psbJ_p3	petA-psbJ	3	AAGAAACAGTTTGAGAAGGTTCA	TTGTGATCGGTTTAATCGGT	23	20	34.8	40.0	56.5	55.1	2.3	0.0	7.405679	63509	64423	714-1083	1.000000	52.0
petA-psbJ_p4	petA-psbJ	4	AGAAACAGTTTGAGAAGGTTCAA	TTGTGATCGGTTTAATCGGT	23	20	34.8	40.0	56.5	55.1	2.3	0.0	7.405679	63509	64423	713-1082	1.000000	52.0
petA-psbJ_p5	petA-psbJ	5	AGAAACAGTTTGAGAAGGTTCA	TCTGGATTAGGTTCATCCCTT	22	21	36.4	42.9	55.8	55.7	2.3	0.0	7.486463	63509	64423	672-1041	1.000000	52.0
ndhF-rpl32_p1	ndhF-rpl32	1	CCGGGTGAACCAAAAACCTA	CGCTGCCCAATATCCTTTCT	20	20	50.0	50.0	58.0	58.0	1.2	0.0	0.043062	112605	113478	1107-1490	1.000000	91.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCGGTGGGAAAAAGTA	CGCTGCCCAATATCCTTTCT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.081360	112605	113478	702-1085	1.000000	91.3
ndhF-rpl32_p3	ndhF-rpl32	3	CCGGGTGAACCAAAAACCTA	AACGCTGCCCAATATCCTTT	20	20	50.0	45.0	58.0	57.9	1.2	0.0	0.159810	112605	113478	1109-1492	1.000000	90.7
ndhF-rpl32_p4	ndhF-rpl32	4	CTCCAACGCGATTAGTCACA	CGCTGCCCAATATCCTTTCT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.036329	112605	113478	1159-1522	0.837209	85.2
ndhF-rpl32_p5	ndhF-rpl32	5	CTCCAACGCGATTAGTCACA	AACGCTGCCCAATATCCTTT	20	20	50.0	45.0	58.0	57.9	15.9	0.0	0.153076	112605	113478	1161-1524	0.837209	84.3
ccsA-ndhD_p1	ccsA-ndhD	1	ATGTAATAACCGCGCCAAGT	GCGCGAATTGTTTCTTTCGA	20	20	45.0	45.0	58.0	58.1	0.0	12.1	0.087473	115741	116013	1071-1109	0.837209	83.5
ccsA-ndhD_p2	ccsA-ndhD	2	GCTGGGCGAGATTCAATACA	GCGCGAATTGTTTCTTTCGA	20	20	50.0	45.0	58.0	58.1	0.0	12.1	0.101234	115741	116013	815-853	0.813953	83.3
ccsA-ndhD_p3	ccsA-ndhD	3	GCTGGGCGAGATTCAATACA	TCAACGGGGCCATTTTACAA	20	20	50.0	45.0	58.0	58.0	0.0	4.4	0.049643	115741	116013	1209-1247	0.395349	64.5
ccsA-ndhD_p4	ccsA-ndhD	4	ATGTAATAACCGCGCCAAGT	TCAACGGGGCCATTTTACAA	20	20	45.0	45.0	58.0	58.0	0.0	4.4	0.035882	115741	116013	1465-1503	0.372093	63.3
ccsA-ndhD_p5	ccsA-ndhD	5	CTGCAATTGTGGCTTCGATG	TCAACGGGGCCATTTTACAA	20	20	50.0	45.0	58.1	58.0	7.4	4.4	0.099058	115741	116013	907-908	0.325581	55.9
ycf1_p1	ycf1	1	TAGCATACCGAAACGACTGC	AGCTCGTACCACAAGAGAGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.170311	124431	124550	1334-1376	0.232558	59.0
ycf1_p2	ycf1	2	TGCTGTGATTAACGAGCGAA	AGCTCGTACCACAAGAGAGT	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.160700	124431	124550	990-990	0.069767	41.5
ycf1_p3	ycf1	3	TTGCTGTGATTAACGAGCGA	AGCTCGTACCACAAGAGAGT	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.160700	124431	124550	991-991	0.069767	41.5
ycf1_p4	ycf1	4	TGCTGTGATTAACGAGCGAA	TCAGCTCGTACCACAAGAGA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.167479	124431	124550	992-992	0.069767	41.5
ycf1_p5	ycf1	5	TTGCTGTGATTAACGAGCGA	TCAGCTCGTACCACAAGAGA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.167479	124431	124550	993-993	0.069767	41.5