primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhB-matK_p1	ndhB-matK	1	TGACAAATTACTAAGTAAAACGA	ACTGCTCTGTTTCATTAACA	23	20	26.1	35.0	52.1	52.7	0.0	0.0	14.125265	81913	82147	291-303	1.000000	45.9
ndhB-matK_p2	ndhB-matK	2	TGACAAATTACTAAGTAAAACGA	AACTGCTCTGTTTCATTAACA	23	21	26.1	33.3	52.1	53.7	0.0	0.0	14.198088	81913	82147	292-304	1.000000	45.9
ndhB-matK_p3	ndhB-matK	3	TGACAAATTACTAAGTAAAACGAA	ACTGCTCTGTTTCATTAACA	24	20	25.0	35.0	53.0	52.7	0.0	0.0	14.291127	81913	82147	291-303	1.000000	45.9
ndhB-matK_p4	ndhB-matK	4	TTGACAAATTACTAAGTAAAACGA	ACTGCTCTGTTTCATTAACA	24	20	25.0	35.0	53.0	52.7	0.0	0.0	14.291127	81913	82147	292-304	1.000000	45.9
ndhB-matK_p5	ndhB-matK	5	TGACAAATTACTAAGTAAAACGA	AAACTGCTCTGTTTCATTAACA	23	22	26.1	31.8	52.1	54.5	0.0	0.0	14.354602	81913	82147	293-305	1.000000	45.8
rps16_p1	rps16	1	AAGTAGGGCAGCAATAGCAA	AGAAATTCATTCTCGGTTTTTAGA	20	24	45.0	29.2	57.2	54.8	0.0	0.0	8.006679	84426	85240	873-976	1.000000	43.8
rps16_p2	rps16	2	AGTAGGGCAGCAATAGCAAA	AGAAATTCATTCTCGGTTTTTAGA	20	24	45.0	29.2	57.2	54.8	0.0	0.0	8.006679	84426	85240	872-975	1.000000	43.8
rps16_p3	rps16	3	AAGTAGGGCAGCAATAGCAAA	AGAAATTCATTCTCGGTTTTTAGA	21	24	42.9	29.2	57.9	54.8	0.0	0.0	8.285687	84426	85240	873-976	1.000000	43.8
rps16_p4	rps16	4	AAGTAGGGCAGCAATAGCAA	GAAATTCATTCTCGGTTTTTAGA	20	23	45.0	30.4	57.2	53.4	0.0	0.0	8.424334	84426	85240	872-975	1.000000	43.8
rps16_p5	rps16	5	AGTAGGGCAGCAATAGCAAA	GAAATTCATTCTCGGTTTTTAGA	20	23	45.0	30.4	57.2	53.4	0.0	0.0	8.424334	84426	85240	871-974	1.000000	43.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	TGTTCGGTTCTGGATTAGCG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	89958	90103	273-283	1.000000	68.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	TGTTCGGTTCTGGATTAGCG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	89958	90103	279-289	1.000000	67.6
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	TGTTCGGTTCTGGATTAGCG	ACCACGTTCAATTCATCTACGT	20	22	50.0	40.9	58.0	58.1	0.0	0.0	2.082906	89958	90103	357-367	1.000000	67.1
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	ACTTCCATGTTCGGTTCTGG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	57.8	56.9	0.0	0.0	2.236609	89958	90103	280-290	1.000000	66.9
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	TGTTCGGTTCTGGATTAGCG	ACGCTCAATAGGATTTGAACCT	20	22	50.0	40.9	58.0	57.8	0.0	0.0	2.226706	89958	90103	308-318	1.000000	66.5
atpH-atpI_p1	atpH-atpI	1	GCAGCAATAACAGAAGCAGC	CTGCGGCTTATATTGGGGAA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.364903	93759	94545	842-857	1.000000	81.4
atpH-atpI_p2	atpH-atpI	2	CGAGACCTACAGCTAACCCA	CTGCGGCTTATATTGGGGAA	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.799233	93759	94545	862-877	1.000000	77.8
atpH-atpI_p3	atpH-atpI	3	CCAGCAGCAATAACAGAAGC	CTGCGGCTTATATTGGGGAA	20	20	50.0	50.0	57.4	57.7	0.0	0.0	0.826886	93759	94545	845-860	1.000000	77.7
atpH-atpI_p4	atpH-atpI	4	AACCCAGCAGCAATAACAGA	CTGCGGCTTATATTGGGGAA	20	20	45.0	50.0	57.4	57.7	0.0	0.0	0.855187	93759	94545	848-863	1.000000	77.5
atpH-atpI_p5	atpH-atpI	5	ACCCAGCAGCAATAACAGAA	CTGCGGCTTATATTGGGGAA	20	20	45.0	50.0	57.4	57.7	0.0	0.0	0.855187	93759	94545	847-862	1.000000	77.5
psbM-trnC-GCA_p1	psbM-trnC-GCA	1	CTCCCGTAAGTTTGCCTACC	CAGACATACCGTTGAGGGTG	20	20	55.0	55.0	58.0	58.0	0.0	5.7	0.030093	111141	111260	861-861	0.333333	53.1
psbM-trnC-GCA_p2	psbM-trnC-GCA	2	CTCCCGTAAGTTTGCCTACC	CTCACCGGTTTGAGATGAGG	20	20	55.0	55.0	58.0	58.0	0.0	15.3	0.035683	111141	111260	912-912	0.333333	53.0
psbM-trnC-GCA_p3	psbM-trnC-GCA	3	CTCCCGTAAGTTTGCCTACC	CATTCTCAGGCTGACTCACC	20	20	55.0	55.0	58.0	58.0	0.0	14.4	0.041303	111141	111260	926-926	0.333333	53.0
psbM-trnC-GCA_p4	psbM-trnC-GCA	4	CTCCCGTAAGTTTGCCTACC	TCAATAGTTGCGATGCTGCT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.048368	111141	111260	754-754	0.333333	52.9
psbM-trnC-GCA_p5	psbM-trnC-GCA	5	CTCCCGTAAGTTTGCCTACC	TTCTCACCCTGCCAGACATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.068738	111141	111260	873-873	0.333333	52.8
trnC-GCA-trnG-GCC_p1	trnC-GCA-trnG-GCC	1	TGCAGTCCTACGTCTTACCA	TCTCTTTGCCAAGGAGATGG	20	20	50.0	50.0	58.1	57.2	0.0	14.6	0.881453	112947	113427	562-589	1.000000	78.1
trnC-GCA-trnG-GCC_p2	trnC-GCA-trnG-GCC	2	TTAGGCGACACCCAGATTTG	TCTCTTTGCCAAGGAGATGG	20	20	50.0	50.0	57.9	57.2	0.0	14.6	0.903181	112947	113427	601-628	1.000000	77.6
trnC-GCA-trnG-GCC_p3	trnC-GCA-trnG-GCC	3	GTTAGGCGACACCCAGATTT	TCTCTTTGCCAAGGAGATGG	20	20	50.0	50.0	57.9	57.2	0.0	14.6	0.904656	112947	113427	602-629	1.000000	77.6
trnC-GCA-trnG-GCC_p4	trnC-GCA-trnG-GCC	4	GCAGTCCTACGTCTTACCAC	TCTCTTTGCCAAGGAGATGG	20	20	55.0	50.0	57.7	57.2	0.0	14.6	1.070151	112947	113427	561-588	1.000000	76.6
trnC-GCA-trnG-GCC_p5	trnC-GCA-trnG-GCC	5	AAGAGTCTTGTCGTTAGGCG	TCTCTTTGCCAAGGAGATGG	20	20	50.0	50.0	57.7	57.2	4.1	14.6	1.144528	112947	113427	614-641	1.000000	75.6
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CCACGAAGACGAAACGATCT	CGTAAGTCGTCGGTTCAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063562	117120	118467	1475-1490	1.000000	83.1
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CCACGAAGACGAAACGATCT	TCTGAAGCCCCCTTAACTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060321	117120	118467	1520-1535	1.000000	83.0
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CGAAACGATCTCTCCTGAGC	CGTAAGTCGTCGGTTCAAGT	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.208244	117120	118467	1466-1481	1.000000	81.9
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CGAAACGATCTCTCCTGAGC	TCTGAAGCCCCCTTAACTCA	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.205003	117120	118467	1511-1526	1.000000	81.8
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	GAAACGATCTCTCCTGAGCC	TCTGAAGCCCCCTTAACTCA	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.256292	117120	118467	1510-1525	1.000000	81.4
trnT-GGU-trnfM-CAU_p1	trnT-GGU-trnfM-CAU	1	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	20	20	50.0	55.0	57.7	58.2	0.0	4.1	0.449537	118540	118962	496-594	1.000000	81.1
trnT-GGU-trnfM-CAU_p2	trnT-GGU-trnfM-CAU	2	ACGACTTACGCCTTACCATG	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.514029	118540	118962	527-625	1.000000	80.3
trnT-GGU-trnfM-CAU_p3	trnT-GGU-trnfM-CAU	3	ACGACTTACGCCTTACCATG	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.514029	118540	118962	526-624	1.000000	80.3
trnT-GGU-trnfM-CAU_p4	trnT-GGU-trnfM-CAU	4	CGACTTACGCCTTACCATGG	CAGGTTCAAATCCTGTCCCC	20	20	55.0	55.0	58.4	58.2	11.2	4.1	0.593072	118540	118962	495-593	1.000000	79.9
trnT-GGU-trnfM-CAU_p5	trnT-GGU-trnfM-CAU	5	ACGACTTACGCCTTACCATG	TGAAGGCATCAACTAAGCGG	20	20	50.0	50.0	57.7	58.3	0.0	0.0	0.551123	118540	118962	561-659	1.000000	79.8
trnS-GGA-trnL-UAA_p1	trnS-GGA-trnL-UAA	1	GTTTAAGGCATAGCACCGGA	CTACCATTCCGCCATATCCC	20	20	50.0	55.0	58.0	57.6	9.5	0.0	0.435932	126728	128184	1548-1731	1.000000	79.1
trnS-GGA-trnL-UAA_p2	trnS-GGA-trnL-UAA	2	CTACCGAGGGTTCAAATCCC	CTACCATTCCGCCATATCCC	20	20	55.0	55.0	57.7	57.6	0.0	0.0	0.730452	126728	128184	1508-1691	1.000000	76.8
trnS-GGA-trnL-UAA_p3	trnS-GGA-trnL-UAA	3	GCTACCGAGGGTTCAAATCC	CTACCATTCCGCCATATCCC	20	20	55.0	55.0	58.3	57.6	0.0	0.0	0.738378	126728	128184	1509-1692	1.000000	76.7
trnS-GGA-trnL-UAA_p4	trnS-GGA-trnL-UAA	4	GAGCGGTTTAAGGCATAGCA	CTACCATTCCGCCATATCCC	20	20	50.0	55.0	58.3	57.6	0.0	0.0	0.739557	126728	128184	1553-1736	1.000000	76.6
trnS-GGA-trnL-UAA_p5	trnS-GGA-trnL-UAA	5	GTTTAAGGCATAGCACCGGA	CGTCTACCATTCCGCCATAT	20	20	50.0	50.0	58.0	57.3	9.5	0.0	0.776316	126728	128184	1551-1734	1.000000	76.4
ndhC-trnM-CAU_p1	ndhC-trnM-CAU	1	GGAGTCATATTGGTGGAGCG	GCGATACTCTAACCGCTGAG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.168247	131147	131537	439-454	1.000000	84.4
ndhC-trnM-CAU_p2	ndhC-trnM-CAU	2	GGAGTCATATTGGTGGAGCG	TTCGAACCAATGACTCTCGC	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.418907	131147	131537	468-483	1.000000	82.0
ndhC-trnM-CAU_p3	ndhC-trnM-CAU	3	GGAGTCATATTGGTGGAGCG	CAATGACTCTCGCCGTATGA	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.620939	131147	131537	461-476	1.000000	80.5
ndhC-trnM-CAU_p4	ndhC-trnM-CAU	4	GGAGTCATATTGGTGGAGCG	CCAATGACTCTCGCCGTATG	20	20	55.0	55.0	58.1	58.5	0.0	0.0	0.637250	131147	131537	462-477	1.000000	80.4
ndhC-trnM-CAU_p5	ndhC-trnM-CAU	5	GGAGTCATATTGGTGGAGCG	TGACTCTCGCCGTATGAAAG	20	20	55.0	50.0	58.1	57.4	0.0	0.0	0.690653	131147	131537	458-473	1.000000	80.0
atpB-rbcL_p1	atpB-rbcL	1	CACATCCAACACTGGTCCAA	GCTTTGAATCCAACACCTGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090407	133652	134299	780-786	1.000000	81.3
atpB-rbcL_p2	atpB-rbcL	2	CACATCCAACACTGGTCCAA	GTCTCCGTTTGTGGTGACAT	20	20	50.0	50.0	58.0	58.1	0.0	9.4	0.141192	133652	134299	756-762	1.000000	81.0
atpB-rbcL_p3	atpB-rbcL	3	CACATCCAACACTGGTCCAA	TTGGTCTTGTACTCAGGGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132424	133652	134299	831-837	1.000000	80.9
atpB-rbcL_p4	atpB-rbcL	4	CACATCCAACACTGGTCCAA	AACACCTGCTTTAGTCTCCG	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.477511	133652	134299	769-775	1.000000	78.3
atpB-rbcL_p5	atpB-rbcL	5	CACATCCAACACTGGTCCAA	CACCTGCTTTAGTCTCCGTT	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.477511	133652	134299	767-773	1.000000	78.3
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	ACCTACGACATCGGGTTTTG	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	57.9	57.9	6.2	0.0	0.217317	146161	146334	285-292	1.000000	81.6
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	ACCTACGACATCGGGTTTTG	CGGTAGCGTGTTTGTTTTGG	20	20	50.0	50.0	57.9	58.3	6.2	6.1	0.339797	146161	146334	286-293	1.000000	80.6
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GAAATAAACACGCCCTGCAG	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	57.7	57.9	6.8	0.0	0.390939	146161	146334	312-319	1.000000	79.9
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	CTCGGAAATAAACACGCCCT	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.394205	146161	146334	316-323	1.000000	79.8
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	TCCTCGGAAATAAACACGCC	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.399084	146161	146334	318-325	1.000000	79.8