primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	ycf37	1	ACCGCTTTTTGTTTTCATACGA	CGGCTTCATGATAGATCCGG	22	20	36.4	55.0	57.4	58.0	0.0	0.0	2.557160	6585	7055	954-954	0.500000	39.5
ycf37_p2	ycf37	2	ACCGCTTTTTGTTTTCATACGA	ACTCAGTCGGTTGGGTAAGA	22	20	36.4	50.0	57.4	58.0	0.0	0.0	2.560718	6585	7055	1255-1255	0.500000	39.5
ycf37_p3	ycf37	3	ACCGCTTTTTGTTTTCATACGA	TTTGTGACTCAGTCGGTTGG	22	20	36.4	50.0	57.4	58.1	0.0	0.0	2.607244	6585	7055	1261-1261	0.500000	39.1
ycf37_p4	ycf37	4	ACCGCTTTTTGTTTTCATACGA	GTCGGTTGGGTAAGAAGACC	22	20	36.4	55.0	57.4	57.9	0.0	0.0	2.645748	6585	7055	1250-1250	0.500000	38.8
ycf37_p5	ycf37	5	ACCGCTTTTTGTTTTCATACGA	TTCAAGGACAAGGCTTAGGC	22	20	36.4	50.0	57.4	57.8	0.0	0.0	2.755721	6585	7055	1075-1075	0.500000	38.0
trnP-ycf52_p1	trnP-ycf52	1	GCTACCAAGCTGCGCTATAT	CGGCTTCATGATAGATCCGG	20	20	50.0	55.0	57.9	58.0	9.7	0.0	0.087866	7156	7307	231-231	0.500000	59.3
trnP-ycf52_p2	trnP-ycf52	2	GCTACCAAGCTGCGCTATAT	ACTCAGTCGGTTGGGTAAGA	20	20	50.0	50.0	57.9	58.0	9.7	0.0	0.091425	7156	7307	532-532	0.500000	59.3
trnP-ycf52_p3	trnP-ycf52	3	GCTACCAAGCTGCGCTATAT	TTTGTGACTCAGTCGGTTGG	20	20	50.0	50.0	57.9	58.1	9.7	0.0	0.137950	7156	7307	538-538	0.500000	58.9
trnP-ycf52_p4	trnP-ycf52	4	GCTACCAAGCTGCGCTATAT	GTCGGTTGGGTAAGAAGACC	20	20	50.0	55.0	57.9	57.9	9.7	0.0	0.176454	7156	7307	527-527	0.500000	58.6
trnP-ycf52_p5	trnP-ycf52	5	GCTACCAAGCTGCGCTATAT	TTCAAGGACAAGGCTTAGGC	20	20	50.0	50.0	57.9	57.8	9.7	0.0	0.286427	7156	7307	352-352	0.500000	57.7
ycf53_p1	ycf53	1	TCATCCAGAGGCTAGTCCAG	GCGAACGACAAAAAGTATGGA	20	21	55.0	42.9	57.9	57.2	6.1	0.0	1.878307	14265	14987	1175-1175	0.500000	45.0
ycf53_p2	ycf53	2	TCATCCAGAGGCTAGTCCAG	AGCGAACGACAAAAAGTATGG	20	21	55.0	42.9	57.9	57.2	6.1	0.0	1.885485	14265	14987	1176-1176	0.500000	44.9
ycf53_p3	ycf53	3	TCAAAATCACGGTTTTGCGG	GCGAACGACAAAAAGTATGGA	20	21	45.0	42.9	57.9	57.2	10.1	0.0	1.926051	14265	14987	1319-1319	0.500000	44.6
ycf53_p4	ycf53	4	TCAAAATCACGGTTTTGCGG	AGCGAACGACAAAAAGTATGG	20	21	45.0	42.9	57.9	57.2	10.1	0.0	1.933229	14265	14987	1320-1320	0.500000	44.5
ycf53_p5	ycf53	5	CAAAATCACGGTTTTGCGGT	GCGAACGACAAAAAGTATGGA	20	21	45.0	42.9	58.2	57.2	2.8	0.0	1.952596	14265	14987	1318-1318	0.500000	44.4
rnz_p1	rnz	1	AATAGCTGCTGGTTCTTGGG	AGAGTTTGAATCACGGCCAT	20	20	50.0	45.0	57.9	57.5	13.4	2.2	0.631687	18043	18717	1597-1598	1.000000	79.8
rnz_p2	rnz	2	CTGCTGGTTCTTGGGCAATA	AGAGTTTGAATCACGGCCAT	20	20	50.0	45.0	58.2	57.5	0.0	2.2	0.658786	18043	18717	1592-1593	1.000000	79.6
rnz_p3	rnz	3	AATAGCTGCTGGTTCTTGGG	CCACCAACATCAATCACTGC	20	20	50.0	50.0	57.9	57.4	13.4	0.0	0.776927	18043	18717	1213-1214	1.000000	78.9
rnz_p4	rnz	4	CTGCTGGTTCTTGGGCAATA	CCACCAACATCAATCACTGC	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.804026	18043	18717	1208-1209	1.000000	78.7
rnz_p5	rnz	5	TGCTGGTTCTTGGGCAATAT	AGAGTTTGAATCACGGCCAT	20	20	45.0	45.0	57.5	57.5	0.0	2.2	1.027358	18043	18717	1591-1592	1.000000	76.7
dsbD_p1	dsbD	1	AATTGCGGACGGAGAGATTC	CACCCTTAGGAACAATCTCCTG	20	22	50.0	50.0	58.1	58.1	0.0	0.0	2.179897	23227	23964	1700-1700	0.500000	42.6
dsbD_p2	dsbD	2	GAATTGCGGACGGAGAGATT	CACCCTTAGGAACAATCTCCTG	20	22	50.0	50.0	58.1	58.1	0.0	0.0	2.179897	23227	23964	1701-1701	0.500000	42.6
dsbD_p3	dsbD	3	AATCCTTCCTGGGGAGTTCA	CACCCTTAGGAACAATCTCCTG	20	22	50.0	50.0	57.9	58.1	0.0	0.0	2.180979	23227	23964	1431-1431	0.500000	42.6
dsbD_p4	dsbD	4	ATCCTTCCTGGGGAGTTCAA	CACCCTTAGGAACAATCTCCTG	20	22	50.0	50.0	57.9	58.1	0.0	0.0	2.180979	23227	23964	1430-1430	0.500000	42.6
dsbD_p5	dsbD	5	TCAAATCCTTCCTGGGGAGT	CACCCTTAGGAACAATCTCCTG	20	22	50.0	50.0	57.9	58.1	0.0	0.0	2.180979	23227	23964	1434-1434	0.500000	42.6
ccs1_p1	ccs1	1	TCGTTCTGTTTACCTAAGCCA	CCGACACCTATGCATGGAAA	21	20	42.9	50.0	57.0	58.0	0.0	12.3	2.070546	24015	25343	1977-1977	0.500000	43.4
ccs1_p2	ccs1	2	TCGTTCTGTTTACCTAAGCCA	AATATCACCAGGACCGGGAA	21	20	42.9	50.0	57.0	58.1	0.0	18.5	2.145792	24015	25343	2122-2122	0.500000	42.8
ccs1_p3	ccs1	3	TCGTTCTGTTTACCTAAGCCA	CTACCGACACCTATGCATGG	21	20	42.9	55.0	57.0	57.8	0.0	10.2	2.188443	24015	25343	1980-1980	0.500000	42.5
ccs1_p4	ccs1	4	TCGTTCTGTTTACCTAAGCCA	TACCGACACCTATGCATGGA	21	20	42.9	50.0	57.0	58.2	0.0	12.3	2.250518	24015	25343	1979-1979	0.500000	42.0
ccs1_p5	ccs1	5	TCGTTCTGTTTACCTAAGCCA	CCGGGAAGACCTGAAGAATC	21	20	42.9	55.0	57.0	57.7	0.0	0.0	2.351119	24015	25343	2109-2109	0.500000	41.2
trpA_p1	trpA	1	TCTTGACCGCCAGAAATAGC	TGTATATGCCCCCATCGTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048351	33540	34349	1200-1200	0.500000	59.6
trpA_p2	trpA	2	CTTGACCGCCAGAAATAGCT	TGTATATGCCCCCATCGTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	33540	34349	1199-1199	0.500000	59.6
trpA_p3	trpA	3	TCTTGACCGCCAGAAATAGC	TATGCCCCCATCGTCTAGAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.099444	33540	34349	1196-1196	0.500000	59.2
trpA_p4	trpA	4	CTTGACCGCCAGAAATAGCT	TATGCCCCCATCGTCTAGAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.105107	33540	34349	1195-1195	0.500000	59.2
trpA_p5	trpA	5	GAGTCTTGACCGCCAGAAAT	TGTATATGCCCCCATCGTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.124780	33540	34349	1203-1203	0.500000	59.0
psaC-ycf22_p1	psaC-ycf22	1	AGCCGGTCAAATAGCTTCAG	ACAGAACCAACTTTCACACCT	20	21	50.0	42.9	58.0	57.6	1.9	0.0	1.395890	46055	46979	1285-1285	0.500000	48.8
psaC-ycf22_p2	psaC-ycf22	2	AATGTGTTCGTGCTTGTCCT	ACAGAACCAACTTTCACACCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.398733	46055	46979	1343-1343	0.500000	48.8
psaC-ycf22_p3	psaC-ycf22	3	ATGTGTTCGTGCTTGTCCTT	ACAGAACCAACTTTCACACCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.398733	46055	46979	1342-1342	0.500000	48.8
psaC-ycf22_p4	psaC-ycf22	4	CAATGTGTTCGTGCTTGTCC	ACAGAACCAACTTTCACACCT	20	21	50.0	42.9	58.0	57.6	0.0	0.0	1.402608	46055	46979	1344-1344	0.500000	48.8
psaC-ycf22_p5	psaC-ycf22	5	TGGTTGTAAAGCCGGTCAAA	ACAGAACCAACTTTCACACCT	20	21	45.0	42.9	57.9	57.6	1.9	0.0	1.420284	46055	46979	1294-1294	0.500000	48.6
ycf22_p1	ycf22	1	GGAGCAGATTTAGTTGAAGTTGG	AGCCATGCCAAAGTTTCTGA	23	20	43.5	45.0	57.7	58.0	0.0	0.0	3.302709	46980	47651	1725-1725	0.500000	33.6
ycf22_p2	ycf22	2	GGAGCAGATTTAGTTGAAGTTGG	TCACCATGCAATTCAACCCA	23	20	43.5	45.0	57.7	58.0	0.0	0.0	3.309163	46980	47651	1595-1595	0.500000	33.5
ycf22_p3	ycf22	3	GGAGCAGATTTAGTTGAAGTTGG	CAATAGCTTCGCCTTGTCCT	23	20	43.5	50.0	57.7	58.0	0.0	0.0	3.332195	46980	47651	1864-1864	0.500000	33.3
ycf22_p4	ycf22	4	GGAGCAGATTTAGTTGAAGTTGG	AGCTTTGCGATATCCACCTG	23	20	43.5	50.0	57.7	58.0	0.0	0.0	3.343316	46980	47651	1749-1749	0.500000	33.3
ycf22_p5	ycf22	5	GGAGCAGATTTAGTTGAAGTTGG	TTCAGCTTCAATACCAGCCC	23	20	43.5	50.0	57.7	57.9	0.0	0.0	3.431128	46980	47651	1839-1839	0.500000	32.6
trnM_p1	trnM	1	ACCTCAAGGAGCAGCTATGA	ATCTCCCTCTGCTGTTGCTA	20	20	50.0	50.0	58.1	58.1	2.3	0.0	0.257165	64660	66522	2634-2647	1.000000	87.1
trnM_p2	trnM	2	ACCTCAAGGAGCAGCTATGA	TATCTCCCTCTGCTGTTGCT	20	20	50.0	50.0	58.1	58.1	2.3	0.0	0.257165	64660	66522	2635-2648	1.000000	87.1
trnM_p3	trnM	3	CCTCAAGGAGCAGCTATGAC	ATCTCCCTCTGCTGTTGCTA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.365975	64660	66522	2633-2646	1.000000	86.3
trnM_p4	trnM	4	CCTCAAGGAGCAGCTATGAC	TATCTCCCTCTGCTGTTGCT	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.365975	64660	66522	2634-2647	1.000000	86.3
trnM_p5	trnM	5	TTCCAAGCTAATGATGCGGG	ATCTCCCTCTGCTGTTGCTA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.449443	64660	66522	2169-2182	1.000000	85.8
ycf58_p1	ycf58	1	ATCAGCAAAAGCCCTTTCCT	AGCACGCGGTTATAAGACAG	20	20	45.0	50.0	57.7	58.1	0.0	12.5	0.393150	67634	68047	1317-1317	0.500000	56.9
ycf58_p2	ycf58	2	ATCAGCAAAAGCCCTTTCCT	GAGATGTTAGCACGCGGTTA	20	20	45.0	50.0	57.7	58.1	0.0	12.5	0.398447	67634	68047	1325-1325	0.500000	56.8
ycf58_p3	ycf58	3	ATCAGCAAAAGCCCTTTCCT	GCACGCGGTTATAAGACAGA	20	20	45.0	50.0	57.7	58.1	0.0	12.5	0.398447	67634	68047	1316-1316	0.500000	56.8
ycf58_p4	ycf58	4	CAGCAAAAGCCCTTTCCTCT	AGCACGCGGTTATAAGACAG	20	20	50.0	50.0	58.4	58.1	0.0	12.5	0.451919	67634	68047	1315-1315	0.500000	56.4
ycf58_p5	ycf58	5	TCAGCAAAAGCCCTTTCCTC	AGCACGCGGTTATAAGACAG	20	20	50.0	50.0	58.4	58.1	0.0	12.5	0.456607	67634	68047	1316-1316	0.500000	56.3
QQP88_pgp117_p1	QQP88_pgp117	1	CGTTTACCGAATTTCGCCAC	ATCCATCGCTAAATGCTGCA	20	20	50.0	45.0	58.1	58.0	13.3	4.1	0.155224	69559	70356	1537-1546	1.000000	87.3
QQP88_pgp117_p2	QQP88_pgp117	2	ATGCGTTTACCGAATTTCGC	ATCCATCGCTAAATGCTGCA	20	20	45.0	45.0	57.8	58.0	13.3	4.1	0.196720	69559	70356	1540-1549	1.000000	86.9
QQP88_pgp117_p3	QQP88_pgp117	3	ACCGAATTTCGCCACTTACT	ATCCATCGCTAAATGCTGCA	20	20	45.0	45.0	57.5	58.0	13.3	4.1	0.496085	69559	70356	1532-1541	1.000000	84.6
QQP88_pgp117_p4	QQP88_pgp117	4	CGTTTACCGAATTTCGCCAC	GATCCATCGCTAAATGCTGC	20	20	50.0	50.0	58.1	57.4	13.3	0.0	0.735172	69559	70356	1538-1547	1.000000	82.6
QQP88_pgp117_p5	QQP88_pgp117	5	ATGCGTTTACCGAATTTCGC	GATCCATCGCTAAATGCTGC	20	20	45.0	50.0	57.8	57.4	13.3	0.0	0.776668	69559	70356	1541-1550	1.000000	82.3
pbsA-bas1_p1	pbsA-bas1	1	AGTTGTTCCTTCACGCAACT	GTCTGGTGGTGTTGGTGATT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.057130	104244	104388	506-506	0.500000	59.5
pbsA-bas1_p2	pbsA-bas1	2	GTTGTTCCTTCACGCAACTG	GTCTGGTGGTGTTGGTGATT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.124066	104244	104388	505-505	0.500000	59.0
pbsA-bas1_p3	pbsA-bas1	3	AGTTGTTCCTTCACGCAACT	GCAACCTGGTCAATCTGCTA	20	20	45.0	50.0	58.0	57.9	0.0	12.1	0.152247	104244	104388	254-254	0.500000	58.8
pbsA-bas1_p4	pbsA-bas1	4	AGTTGTTCCTTCACGCAACT	AATCCTGATGAAGTGTGCCC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.173176	104244	104388	283-283	0.500000	58.6
pbsA-bas1_p5	pbsA-bas1	5	AGTTGTTCCTTCACGCAACT	TGGTTCAAATCCATTCCCCG	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.192380	104244	104388	928-928	0.500000	58.5
trnS.2-trnM.3_p1	trnS.2-trnM.3	1	TAGCAATCTGCCGCTTTCAA	GACTCATAAGCCCAAGGTCG	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.238747	140281	140302	105-105	1.000000	81.9
trnS.2-trnM.3_p2	trnS.2-trnM.3	2	TTAGCAATCTGCCGCTTTCA	GACTCATAAGCCCAAGGTCG	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.238747	140281	140302	106-106	1.000000	81.9
trnS.2-trnM.3_p3	trnS.2-trnM.3	3	AGATTAGCAATCTGCCGCTT	GACTCATAAGCCCAAGGTCG	20	20	45.0	55.0	57.7	58.1	9.6	0.0	0.397252	140281	140302	109-109	1.000000	80.5
trnS.2-trnM.3_p4	trnS.2-trnM.3	4	TAGCAATCTGCCGCTTTCAA	GGACTCATAAGCCCAAGGTC	20	20	45.0	55.0	58.2	57.7	0.0	0.0	0.513760	140281	140302	106-106	1.000000	79.7
trnS.2-trnM.3_p5	trnS.2-trnM.3	5	TTAGCAATCTGCCGCTTTCA	GGACTCATAAGCCCAAGGTC	20	20	45.0	55.0	58.2	57.7	0.0	0.0	0.513760	140281	140302	107-107	1.000000	79.6
dnaB_p1	dnaB	1	TCAGTCCACTGCTCTACCAA	GCATCACACTTTTTAGCCAACA	20	22	50.0	40.9	58.0	58.0	1.5	0.0	2.033513	146903	148660	2511-2511	0.500000	43.7
dnaB_p2	dnaB	2	TCCACTGCTCTACCAACTGA	GCATCACACTTTTTAGCCAACA	20	22	50.0	40.9	58.0	58.0	0.0	0.0	2.033513	146903	148660	2507-2507	0.500000	43.7
dnaB_p3	dnaB	3	TTCAGTCCACTGCTCTACCA	GCATCACACTTTTTAGCCAACA	20	22	50.0	40.9	58.0	58.0	1.5	0.0	2.033513	146903	148660	2512-2512	0.500000	43.7
dnaB_p4	dnaB	4	GGAGATAGCGCTGTTGTTGA	GCATCACACTTTTTAGCCAACA	20	22	50.0	40.9	58.0	58.0	22.9	0.0	2.033976	146903	148660	2721-2721	0.500000	43.7
dnaB_p5	dnaB	5	AGGAGATAGCGCTGTTGTTG	GCATCACACTTTTTAGCCAACA	20	22	50.0	40.9	58.0	58.0	22.9	0.0	2.039567	146903	148660	2722-2722	0.500000	43.7
moeB_p1	moeB	1	ACCCAAAAATAGTTCTTCTGCT	CTACGTGCTGTGCCACTAAT	22	20	36.4	50.0	55.9	58.0	0.0	0.0	4.121474	149270	150331	2349-2349	0.500000	27.0
moeB_p2	moeB	2	ACCCAAAAATAGTTCTTCTGCT	AACAGAAGCTGGAGGAGTCT	22	20	36.4	50.0	55.9	58.0	0.0	0.0	4.134047	149270	150331	2179-2179	0.500000	26.9
moeB_p3	moeB	3	ACCCAAAAATAGTTCTTCTGCT	ACAGAAGCTGGAGGAGTCTT	22	20	36.4	50.0	55.9	58.0	0.0	0.0	4.134047	149270	150331	2178-2178	0.500000	26.9
moeB_p4	moeB	4	ACCCAAAAATAGTTCTTCTGCT	CTCCATGTTGACCTAACGCA	22	20	36.4	50.0	55.9	57.9	0.0	0.0	4.204635	149270	150331	2045-2045	0.500000	26.4
moeB_p5	moeB	5	ACCCAAAAATAGTTCTTCTGCT	TCCATGTTGACCTAACGCAG	22	20	36.4	50.0	55.9	57.9	0.0	0.0	4.204635	149270	150331	2044-2044	0.500000	26.4
rps1_p1	rps1	1	TCCATTGCCGTAACCTCTTG	GGCGGTATATTTCTGTGGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.146156	155163	155978	1283-1299	1.000000	87.1
rps1_p2	rps1	2	TCCATTGCCGTAACCTCTTG	ATCTGGTACGCTCTTACCGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.156394	155163	155978	1331-1347	1.000000	86.9
rps1_p3	rps1	3	TCCATTGCCGTAACCTCTTG	AAACATAAGCCGGGTTTGGT	20	20	50.0	45.0	57.9	58.0	0.0	26.0	0.111887	155163	155978	1563-1563	0.500000	59.1
rps1_p4	rps1	4	TCCATTGCCGTAACCTCTTG	AACATAAGCCGGGTTTGGTT	20	20	50.0	45.0	57.9	58.0	0.0	26.0	0.111887	155163	155978	1562-1562	0.500000	59.1
rps1_p5	rps1	5	TGGAGTCACAATCCATTGCC	AACATAAGCCGGGTTTGGTT	20	20	50.0	45.0	58.2	58.0	0.0	26.0	0.165825	155163	155978	1573-1573	0.500000	58.7
syfB_p1	syfB	1	TGCCACAGAAATATACCGCC	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.055111	156441	158459	2765-2774	1.000000	87.8
syfB_p2	syfB	2	TCGGTAAGAGCGTACCAGAT	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	58.0	58.0	12.0	0.0	0.065349	156441	158459	2717-2726	1.000000	87.7
syfB_p3	syfB	3	AACCAAACCCGGCTTATGTT	CGAATCCTCCTACCCCAGAT	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.020842	156441	158459	2511-2511	0.500000	59.8
syfB_p4	syfB	4	ACCAAACCCGGCTTATGTTT	CGAATCCTCCTACCCCAGAT	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.020842	156441	158459	2510-2510	0.500000	59.8
syfB_p5	syfB	5	ACCAAACCCGGCTTATGTTT	CGTGATGGTAGTACAGGCAC	20	20	45.0	55.0	58.0	58.1	0.0	2.1	0.081367	156441	158459	2366-2366	0.500000	59.3