primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	479	517-538	1.000000	79.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	479	531-552	1.000000	78.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	479	530-551	1.000000	78.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	479	532-553	1.000000	78.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	479	518-539	1.000000	78.5
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	57.8	57.9	0.0	0.0	0.376342	1542	1759	420-420	1.000000	78.1
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	57.8	57.8	0.0	18.0	0.464081	1542	1759	335-335	1.000000	77.7
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.437992	1542	1759	421-421	1.000000	77.6
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	20	20	50.0	55.0	57.7	57.8	0.0	18.0	0.525731	1542	1759	336-336	1.000000	77.2
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TTTTCGGTGCTGGTTATCCA	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	57.4	57.9	0.0	0.0	0.703155	1542	1759	411-411	1.000000	75.5
trnK-UUU_p1	trnK-UUU	1	GCCCGTGTCAACCAATATCT	TTCGTCGGATTCGCTTTGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.042969	1760	4385	2975-2998	1.000000	80.8
trnK-UUU_p2	trnK-UUU	2	TGCCCGTGTCAACCAATATC	TTCGTCGGATTCGCTTTGAT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.259351	1760	4385	2976-2999	1.000000	79.1
trnK-UUU_p3	trnK-UUU	3	GCCCGTGTCAACCAATATCT	GGATTCGCTTTGATACCCGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.272687	1760	4385	2969-2992	1.000000	79.0
trnK-UUU_p4	trnK-UUU	4	GCCCGTGTCAACCAATATCT	TCGGATTCGCTTTGATACCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.272687	1760	4385	2971-2994	1.000000	79.0
trnK-UUU_p5	trnK-UUU	5	ATGCCCGTGTCAACCAATAT	TTCGTCGGATTCGCTTTGAT	20	20	45.0	45.0	57.6	58.0	0.0	0.0	0.439955	1760	4385	2977-3000	1.000000	77.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4386	5198	1010-1010	1.000000	77.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4386	5198	1009-1009	1.000000	77.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4386	5198	949-949	1.000000	76.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4386	5198	948-948	1.000000	76.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4386	5198	1034-1034	1.000000	76.3
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	20	20	50.0	50.0	57.9	57.1	1.4	0.0	0.989589	7880	8288	546-546	1.000000	72.7
psbK-psbI_p2	psbK-psbI	2	TATGCCAGTCATACCCCTGT	TCTTCACGTCCAGGATTACG	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.123894	7880	8288	586-586	1.000000	71.6
psbK-psbI_p3	psbK-psbI	3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	7880	8288	543-543	1.000000	70.7
psbK-psbI_p4	psbK-psbI	4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	7880	8288	544-544	1.000000	70.6
psbK-psbI_p5	psbK-psbI	5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	7880	8288	545-545	1.000000	70.6
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.042943	8400	8533	249-250	1.000000	73.3
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	20	20	50.0	55.0	57.1	57.6	0.0	0.0	1.329655	8400	8533	197-198	1.000000	71.4
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.335793	8400	8533	249-250	1.000000	70.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	21	20	52.4	50.0	57.7	58.1	0.0	0.0	1.434740	8400	8533	268-269	1.000000	70.0
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	20	20	50.0	55.0	57.1	58.6	0.0	0.0	1.569752	8400	8533	240-241	1.000000	69.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9458	10263	862-864	1.000000	78.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9458	10263	880-882	1.000000	76.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9458	10263	861-863	1.000000	72.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9458	10263	870-872	1.000000	72.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9458	10263	919-921	1.000000	71.8
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	CTTGAACCCTCGTTGTCTCC	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.2	56.8	0.0	0.0	2.417098	12127	12984	998-998	1.000000	63.5
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	CTTGAACCCTCGTTGTCTCC	TCAAACCCTGATCAATGAACCT	20	22	55.0	40.9	58.2	57.6	0.0	0.0	2.590002	12127	12984	988-988	1.000000	62.2
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	AAACCTCTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.4	56.8	25.5	0.0	2.617627	12127	12984	959-959	1.000000	62.1
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	CTTGAACCCTCGTTGTCTCC	TCTTCTGTCAAACCCTGATCA	20	21	55.0	42.9	58.2	56.6	0.0	0.0	2.628580	12127	12984	995-995	1.000000	61.9
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	CGTTGTCTCCGTGAAAGAGA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	57.6	56.8	0.0	0.0	2.631461	12127	12984	988-988	1.000000	61.9
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	25166	26293	1264-1264	1.000000	77.8
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	25166	26293	1320-1320	1.000000	77.2
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	25166	26293	1280-1280	1.000000	76.9
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	25166	26293	1265-1265	1.000000	76.6
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	25166	26293	1324-1324	1.000000	76.6
psaA-ycf3_p1	psaA-ycf3	1	GAGAAATGACCCGGTCTAGC	GGAAACAGGCTATAGCGCTT	20	20	55.0	50.0	57.8	58.0	18.5	18.7	0.271675	41400	42147	904-926	1.000000	79.8
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.487535	41400	42147	918-940	1.000000	78.1
psaA-ycf3_p3	psaA-ycf3	3	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.488764	41400	42147	920-942	1.000000	78.1
psaA-ycf3_p4	psaA-ycf3	4	GAGAAATGACCCGGTCTAGC	AGGCTATAGCGCTTACTCCT	20	20	55.0	50.0	57.8	57.7	18.5	18.7	0.522675	41400	42147	898-920	1.000000	77.9
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.529992	41400	42147	839-861	1.000000	77.9
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	60483	61327	1025-1031	1.000000	79.5
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	60483	61327	957-963	1.000000	79.0
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	60483	61327	989-995	1.000000	78.9
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	60483	61327	973-979	1.000000	78.5
ycf4-cemA_p5	ycf4-cemA	5	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.426882	60483	61327	1024-1030	1.000000	76.9
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	63207	64001	910-933	1.000000	79.1
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	63207	64001	900-923	1.000000	79.0
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	63207	64001	948-971	1.000000	77.3
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	63207	64001	947-970	1.000000	77.3
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	63207	64001	938-961	1.000000	77.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	64793	66067	1467-1469	1.000000	72.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	64793	66067	1390-1392	1.000000	71.5
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	64793	66067	1476-1478	1.000000	71.4
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	64793	66067	1481-1483	1.000000	71.3
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	64793	66067	1475-1477	1.000000	71.2
petD-rpoA_p1	petD-rpoA	1	TTGGTTGGGTATTGGTGCAA	TGGAGCATTTTCGCATAGAAGA	20	22	45.0	40.9	57.9	58.1	0.0	0.0	2.215408	77858	78053	344-346	1.000000	63.9
petD-rpoA_p2	petD-rpoA	2	TTTGGTTGGGTATTGGTGCA	TGGAGCATTTTCGCATAGAAGA	20	22	45.0	40.9	57.9	58.1	0.0	0.0	2.215408	77858	78053	345-347	1.000000	63.9
petD-rpoA_p3	petD-rpoA	3	TTGGTTGGGTATTGGTGCAA	TGGAGCATTTTCGCATAGAAG	20	21	45.0	42.9	57.9	56.7	0.0	0.0	2.403831	77858	78053	344-346	1.000000	62.4
petD-rpoA_p4	petD-rpoA	4	TTTGGTTGGGTATTGGTGCA	TGGAGCATTTTCGCATAGAAG	20	21	45.0	42.9	57.9	56.7	0.0	0.0	2.403831	77858	78053	345-347	1.000000	62.4
petD-rpoA_p5	petD-rpoA	5	CCAGTAGCGACAACCGTATT	TGGAGCATTTTCGCATAGAAGA	20	22	50.0	40.9	57.7	58.1	0.0	0.0	2.416264	77858	78053	388-390	1.000000	62.1
ycf1_p1	ycf1	1	ACGACGAAAAGAACAAGGCA	TCGAGGAGGACCAAAAGGAT	20	20	45.0	50.0	58.3	58.0	0.0	0.0	0.388385	110900	111019	829-829	1.000000	77.9
ycf1_p2	ycf1	2	ACGACGAAAAGAACAAGGCA	GCGGAATGCTATCCCAGATT	20	20	45.0	50.0	58.3	57.8	0.0	3.2	0.532134	110900	111019	443-443	1.000000	77.4
ycf1_p3	ycf1	3	ACGAAAAGAACAAGGCATGC	TCGAGGAGGACCAAAAGGAT	20	20	45.0	50.0	57.6	58.0	9.9	0.0	0.469654	110900	111019	826-826	1.000000	77.2
ycf1_p4	ycf1	4	ACGACGAAAAGAACAAGGCA	TCGTCCAACCTGTAGATCGA	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.459319	110900	111019	1121-1121	1.000000	77.1
ycf1_p5	ycf1	5	AACAAGGCATGCTACAAGGG	TCGAGGAGGACCAAAAGGAT	20	20	50.0	50.0	58.5	58.0	18.9	0.0	0.498175	110900	111019	818-818	1.000000	77.0
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	123722	124597	1008-1024	1.000000	77.8
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	123722	124597	1009-1025	1.000000	77.8
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	123722	124597	991-1007	1.000000	77.6
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	123722	124597	992-1008	1.000000	77.6
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.489250	123722	124597	1018-1034	1.000000	76.9
rpl32-ndhF_p1	rpl32-ndhF	1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.6	58.0	0.0	17.5	0.397386	124763	125800	1218-1242	1.000000	77.5
rpl32-ndhF_p2	rpl32-ndhF	2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	124763	125800	1216-1240	1.000000	77.4
rpl32-ndhF_p3	rpl32-ndhF	3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	124763	125800	1217-1241	1.000000	77.4
rpl32-ndhF_p4	rpl32-ndhF	4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.6	58.0	9.5	17.5	0.425347	124763	125800	1235-1259	1.000000	77.3
rpl32-ndhF_p5	rpl32-ndhF	5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	20	20	45.0	45.0	57.6	58.0	9.5	17.5	0.436636	124763	125800	1233-1257	1.000000	77.2